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ABSTRACT: Trichothecene mycotoxins are commonly distributed in crop fields in the world, and zearalenone has been found in cereals. Rapid and accurate methods for the determination of these mycotoxins is required to prevent the intoxication of humans, and to contribute to the supply of safe foods and feeds for human and livestock. Gas chromatography-mass spectrometry (GC-MS) is a useful method for the determination of these toxins. We describe here our current GC-MS analysis for the simultaneous determination of trichothecenes and zearalenone contaminants in cereals.
Journal of Chromatography 07/2000; 882(1-2):23-8. · 4.53 Impact Factor
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ABSTRACT: A survey for the natural occurrence of Fusarium mycotoxins, deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEN), in Dutch cereals (totaling 29 samples) harvested in 1984/1985, showed that 90%, 79% and 62% of samples were contaminated with DON, NIV and ZEN, respectively. Average contents (ng/g) in the total of positive samples were 221 (DON), 123 (NIV) and 61 (ZEN). Among the cereals examined, the highest concentrations (ng/g) was 3198 (DON), 1875 (NIV) and 677 (ZEN) in a yellow corn sample for animal feed. The results of this survey show that Dutch cereals were relatively significantly contaminated with Fusarium mycotoxins.
Mycopathologia 05/1990; 110(1):19-22. · 1.65 Impact Factor
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ABSTRACT: An investigation for the occurrence of nivalenol (NIV), deoxynivalenol (DON) and zearalenone (ZEN) in cereals (ten wheat, one rye and one corn) harvested in Canada have been carried out using a procedure, which is rapid and sensitive for Fusarium mycotoxins. NIV, DON and ZEN were detected in 4, 9 and 9 out of ten wheat samples, and their average levels in the positives were 23 ng/g, 1257 ng/g and 9 ng/g, respectively. One rye and one corn were also contaminated with a minor amount of NIV. This is the first evidence for the natural occurrence of NIV in cereals grown in Canada, though its level was far less than DON.
Mycopathologia 04/1988; 101(3):157-60. · 1.65 Impact Factor