Publications (2)0 Total impact
Article: Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova.[show abstract] [hide abstract]
ABSTRACT: To investigate the physiological importance of somatotrophin release-inhibiting factor (SRIF), effects in vitro of synthetic SRIF 14 on germinal vesicle breakdown (GVB) of cultured porcine follicular ova were studied. The proportion of ova with GVB decreased gradually and significantly with increasing concentrations of SRIF 14 in the range from 6 X 10(-12) to 6 X 10(-7) M during a 22 h period of culture. The inhibitory effect was apparent for the period between 14 and 22 h in the course of culture but was reversed by a concomitant addition of anti-SRIF to the medium. Neither synthetic oxytocin, vasoactive intestinal polypeptide nor substance P exerted any inhibitory or stimulatory action on GVB. These results suggest a limited but definite inhibitory action of SRIF on GVB of porcine ova.Acta endocrinologica 12/1985; 110(3):408-12.
Article: Evidence for existence of somatostatin-like immunoreactivity with molecular heterogeneity in porcine ovaries.[show abstract] [hide abstract]
ABSTRACT: Measurement of somatostatin (SRIF) by RIA in porcine ovarian tissues after removal of the follicular fluid revealed the existence of immunoreactive (IR) SRIF at concentrations of 2.2 +/- 0.6 ng/g wet weight (mean +/- SD). On gel chromatographic analysis of ovarian extracts one major and two minor components with SRIF-like immunoreactivities have been detected. The major component comprised 59.5 +/- 15.1% of the total IR-SRIF and was coeluted with synthetic tetradecapeptide (SRIF 14). The two other components seemed to have almost the same molecular weights as synthetic octacosapeptide (SRIF 28) (25.1 +/- 10%) and pro-somatostatin (15.4 +/- 5.5%), respectively. It is concluded that SRIF in porcine ovaries is heterogeneous, comprising SRIF 14 as a major component, as well as larger forms of SRIF which probably represent SRIF precursors.Acta endocrinologica 07/1984; 106(2):254-9.