Sun Young Kim

Samsung Medical Center, Sŏul, Seoul, South Korea

Are you Sun Young Kim?

Claim your profile

Publications (389)1026.96 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the therapeutic effect of oral steroids given to patients younger than 3 years with epidemic keratoconjunctivitis (EKC) accompanied by severe eyelid edema and inflammatory ptosis, in whom eye drops were not feasible. This study included 9 patients treated for EKC in local clinics whose condition failed to improve due to severe eyelid swelling together with difficulties in application of eye drops and pseudomembrane removal. We analyzed the extent of eyelid swelling, corneal damage, follicles, chemosis, and pseudomembrane formation in these patients before and after oral corticosteroid therapy in collaboration with the pediatrics department. After a mean of 1.8 ± 0.7 days of oral steroid treatment, eyelid edema, corneal damage, conjunctival injection, follicles, and chemosis improved in all patients. Oral steroids are an effective adjuvant treatment for EKC in patients younger than 3 years in whom eye drops could not be administered frequently due to severe eyelid edema.
    Cornea 12/2014; · 1.75 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: We report updated progression-free survival (PFS) and overall survival (OS) data from a trial that compared capecitabine plus oxaliplatin (CapeOX) versus S-1 plus oxaliplatin (SOX) for the first-line treatment of metastatic colorectal cancer. This trial was a randomized, two-armed, non-inferiority phase 3 comparison of CapeOX (capecitabine 1000 mg/m2 twice daily on days 1-14 and oxaliplatin 130 mg/m2 on day 1) versus SOX (S-1 40mg/m2 twice daily on days 1-14 and oxaliplatin 130 mg/m2 on day 1). The primary end point was to show non-inferiority of SOX relative to CapeOX in terms of PFS. Thus, a follow-up exploratory analysis of PFS and OS was performed. The intention to treat (ITT) population was comprised of 340 patients (SOX arm: 168 and CapeOX arm: 172). The updated median PFS was 7.1 months (95% CI 6.4-8.0) in the SOX group and 6.3 months (95% CI 4.9-6.7) in the CapeOX group (hazard ratio [HR], 0.83 [0.66-1.04], p = .10). The median OS was 19.0 months (95% CI 15.3-23.0) in the SOX group and 18.4 months (95% CI 14.1-20.7) in the CapeOX group (HR, 0.86 [0.68-1.08], p = .19). Subgroup analyses according to principal demographic factors such as sex, age, ECOG (Eastern Cooperative Oncology Group) performance status, primary tumor location, measurability, previous adjuvant therapy, number of metastatic organs, and liver metastases showed no interaction between any of these characteristics and the treatment. Updated survival analysis shows that SOX is similar to CapeOX, confirming the initial PFS analysis. Therefore, the SOX regimen could be an alternative first-line doublet chemotherapy strategy for patients with metastatic colorectal cancer.Trial registration: NCT00677443 and May 12 2008.
    BMC Cancer 11/2014; 14(1):883. · 3.33 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: ABSTRACT A 3.5 yr old spayed female Staffordshire terrier weighing 25.5 kg was presented with a 7 wk history of bilateral plantigrade stance in the pelvic limbs directly following an ovariohysterectomy procedure. Upon presentation, the dog had bilateral atrophy of the distal pelvic limb muscles, enlarged popliteal lymph nodes, and ulcerative wounds on the dorsa of her rear paws. Orthopedic examination revealed intact calcaneal tendons bilaterally and neurologic examination localized the lesion to the distal sciatic nerve. A diagnosis of compressive and stretch neuropathy was made affecting the distal sciatic nerve branches. Physical therapy modalities included neuromuscular electrical stimulation, ultrasound, and low-level laser therapy. Other therapeutic modalities included the use of orthotics and progressive wound care. The dog had increased muscle mass, return of segmental reflexes, return of nociception, and the ability to walk on pelvic limbs with higher carriage of the hock 15 mo following presentation. The use of custom orthotics greatly increased the quality of life and other physical therapy modalities may have improved the prognosis in this dog with severe bilateral plantigrade stance due to neuropathy.
    Journal of the American Animal Hospital Association 11/2014; · 0.76 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Protein tyrosine phosphatases (PTPs) have been recognized as critical components of multiple signaling regulators of fundamental cellular processes, including differentiation, cell death and migration. Here, we show that dual-specificity phosphatase 4 (DUSP4) is crucial for neuronal differentiation and functions in the neurogenesis of embryonic stem cells. The endogenous mRNA and protein expression levels of DUSP4 gradually increased during mouse development from embryonic stem cells to postnatal stages. Neurite outgrowth and the expression of neuron-specific markers were markedly reduced by genetic ablation of DUSP4 in differentiated neurons, and these effects were rescued by the reintroduction of DUSP4. In addition, DUSP4 knockdown dramatically enhanced extracellular signal-regulated kinase (ERK) activation during neuronal differentiation. Furthermore, the DUSP4-ERK pathway functioned to balance calcium signaling, not only by regulating Ca2+/calmodulin-dependent kinase I (CaMKI) phosphorylation but also by facilitating Cav1.2 expression and plasma membrane localization. These data are the first to suggest a molecular link between the MAPK-ERK cascade and calcium signaling, which provides insight into the mechanism by which DUSP4 modulates neuronal differentiation.
    Stem cells and development. 11/2014;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to compare the clinical outcomes of laparoscopic surgery with those of open surgery in patients with colorectal cancer and unresectable metastasis.
    Surgery Today 11/2014; · 0.96 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To perform chemoradiotherapy (CRT) effectively, it is clinically beneficial to identify predictors of tumor response after CRT. This study examined the association between plasma fibrinogen level before preoperative CRT and tumor response in advanced rectal cancer.
    Annals of Surgical Oncology 11/2014; · 4.12 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background The role of adjuvant chemotherapy for patients with rectal cancer is controversial, especially when used after preoperative chemoradiotherapy. Fluoropyrimidine-based adjuvant chemotherapy, including fluorouracil and leucovorin, has been widely used; however, the addition of oxaliplatin to fluorouracil and leucovorin (FOLFOX), a standard adjuvant regimen for colon cancer, has not been tested in rectal cancer. We aimed to compare the efficacy and safety of adjuvant fluorouracil and leucovorin with that of FOLFOX in patients with locally advanced rectal cancer after preoperative chemoradiotherapy. Methods In this open-label, multicentre, phase 2, randomised trial, patients with postoperative pathological stage II (ypT3–4N0) or III (ypTanyN1–2) rectal cancer after preoperative fluoropyrimidine-based chemoradiotherapy and total mesorectal excision were recruited and randomly assigned (1:1) via a web-based software platform to receive adjuvant chemotherapy with either four cycles of fluorouracil and leucovorin (fluorouracil 380 mg/m2 and leucovorin 20 mg/m2 on days 1–5, every 4 weeks) or eight cycles of FOLFOX (oxaliplatin 85 mg/m2, leucovorin 200 mg/m2, and fluorouracil bolus 400 mg/m2 on day 1, and fluorouracil infusion 2400 mg/m2 for 46 h, every 2 weeks). Stratification factors were pathological stage (II vs III) and centre. Neither patients nor investigators were masked to group assignment. The primary endpoint was 3-year disease-free survival, analysed by intention to treat. This study is fully enrolled, is in long-term follow-up, and is registered with, number NCT00807911. Findings Between Nov 19, 2008, and June 12, 2012, 321 patients were randomly assigned to fluorouracil and leucovorin (n=161) and FOLFOX (n=160). 141 (95%) of 149 patients in the fluorouracil plus leucovorin group and 141 (97%) of 146 in the FOLFOX group completed all planned cycles of adjuvant treatment. Median follow-up was 38·2 months (IQR 26·4–50·6). 3-year disease-free survival was 71·6% (95% CI 64·6–78·6) in the FOLFOX group and 62·9% (55·4–70·4) in the fluorouracil plus leucovorin group (hazard ratio 0·657, 95% CI 0·434–0·994; p=0·047). Any grade neutropenia, thrombocytopenia, fatigue, nausea, and sensory neuropathy were significantly more common in the FOLFOX group than in the fluorouracil plus leucovorin group; however, we noted no significant difference in the frequency of these events at grade 3 or 4. The most common grade 3 or worse adverse events were neutropenia (38 [26%] of 149 patients in the fluorouracil plus leucovorin group vs 52 [36%] of 146 patients in the FOLFOX group), leucopenia (eight [5%] vs 12 [8%]), febrile neutropenia (four [3%] vs one [<1%]), diarrhoea (four [3%] vs two [1%]), and nausea (one [<1%] vs two [1%]). Interpretation Adjuvant FOLFOX improves disease-free survival compared with fluorouracil plus leucovorin in patients with locally advanced rectal cancer after preoperative chemoradiotherapy and total mesorectal excision, and warrants further investigation. Funding Korea Healthcare Technology R&D Project (South Korean Ministry of Health and Welfare).
    The Lancet Oncology 10/2014; · 25.12 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases. Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginseng extract, and ginsenoside contents, skin permeability, and chemical compositions including total sugar, acidic polysaccharide, and polyphenols were determined on the hydrolyzed ginseng extract. Rapidase treatment significantly elevated total ginsenoside contents compared with a control (p<0.05). Specially, deglycosylated ginsenosides including Rg3, which are known as bioactive compounds, were significantly increased after Rapidase treatment (p<0.05). Rapidase-treated group also increased the skin permeability of polyphenols compared to the control, showing the highest level of total sugar content among enzyme treatment groups. This result showed that Rapidase induced the conversion of ginsenoside glycosides to algycones. Meanwhile, Cytolase PCL5 and Econitase treatments led to a significant increase of uronic acid (acidic polysaccharide) level. Taken together, our data showed that the treatments of enzymes including Rapidase are useful for the conversion and increase of ginsenosides in ginseng extracts or products.
    Journal of ginseng research 10/2014; · 2.26 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.
    Molecules and cells. 09/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background & Aims: Patients with Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) have a better prognosis than those with gastric cancer not associated with EBV infection (EBVnGC). This is partly because EBV infection recruits lymphocytes, which infiltrate the tumor. A high degree of tumor heterogeneity is likely to be associated with poor response. We investigated differences in gene expression patterns between EBVaGC and EBVnGC. Methods We used gene expression profile analysis to compare tumor and non-tumor gastric tissues from 12 patients with EBVaGC and 14 patients with EBVnGC. Findings were validated by whole transcriptome RNAseq and real-time quantitative PCR analyses. CD3+ primary T cells were isolated from human blood samples; migration of these cells and of Jurkat cells were measured in culture with EBV-infected and uninfected gastric cancer cells. Results Based on Pearson correlation matrix analysis, EBVaGCs had a higher degree of homogeneity than EBVnGCs. Although 4550 genes were differentially expressed between tumor and non-tumor gastric tissues of patients with EBVnGC, only 186 genes were differentially expressed between tumor and non-tumor gastric tissues of patients with EBVaGC (P<.001). This finding supports the concept that EBVaGCs have fewer genetic and epigenetic alterations than EBVnGCs. Expression of MHC-class II genes and genes that regulate chemokine activity were more often deregulated in EBVaGCs, compared with non-tumor tissues. In culture, more T cells migrated to EBV-infected gastric cancer cells than to uninfected cells; migration was blocked with a neutralizing antibody against CXCR3 (a receptor for many chemokines). Conclusions Fewer genes are deregulated in EBVaGC than in EBVnGC. Most changes in EBVaGCs occur in immune response genes. These changes might allow EBVaGC to recruit reactive immune cells; this might contribute to the better outcomes of these patients, compared to those with EBVnGC.
    Gastroenterology 09/2014; · 12.82 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background and purpose The reported overall accuracy of MRI in predicting the pathologic stage of nonirradiated rectal cancer is high. However, the role of MRI in restaging rectal tumors after neoadjuvant CRT is contentious. Thus, we evaluate the accuracy of restaging magnetic resonance imaging (MRI) for rectal cancer patients who receive preoperative chemoradiotherapy (CRT). Methods and materials We analyzed 150 patients with locally advanced rectal cancer (T3–4N0–2) who had received preoperative CRT. Pre-CRT MRI was performed for local tumor and nodal staging. All patients underwent restaging MRI followed by total mesorectal excision after the end of radiotherapy. The primary endpoint of the present study was to estimate the accuracy of post-CRT MRI as compared with pathologic staging. Results Pathologic T classification matched the post-CRT MRI findings in 97 (64.7%) of 150 patients. 36 (24.0%) of 150 patients were overstaged in T classification, and the concordance degree was moderate (k = 0.33, p < 0.01). Pathologic N classification matched the post-CRI MRI findings in 85 (56.6%) of 150 patients. 54 (36.0%) of 150 patients were overstaged in N classification. 26 patients achieved downstaging (ycT0–2N0) on restaging MRI after CRT. 23 (88.5%) of 26 patients who had been downstaged on MRI after CRT were confirmed on the pathological staging, and the concordance degree was good (k = 0.72, p < 0.01). Conclusions Restaging MRI has low accuracy for the prediction of the pathologic T and N classifications in rectal cancer patients who received preoperative CRT. The diagnostic accuracy of restaging MRI is relatively high in rectal cancer patients who achieved clinical downstaging after CRT.
    Radiotherapy and Oncology. 09/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: The aim of this study was to evaluate the association between the efficacy of first-line cytotoxic chemotherapy plus bevacizumab and single-nucleotide polymorphisms (SNPs) of angiogenic genes in patients with advanced colorectal cancer (CRC). Methods: DNA was extracted from blood samples of 125 patients, and 12 SNPs were evaluated for association with the objective response rate (ORR), progression-free survival (PFS), and overall survival (OS). Results: The vascular endothelial growth factor A (VEGFA) rs833061 T/T was associated with superior ORR compared to its alternative genotypes (75.9 vs. 50.8%; p = 0.008), and the interleukin 8 rs4073 A/A genotype tended to be associated with poor ORR (45.0 vs. 66.0%; p = 0.067). The median PFS and OS were superior in patients with the fms-related tyrosine kinase 1 (FLT1) rs9513070 A/A genotype (8.7 vs. 6.6 months; p = 0.001 and 26.4 vs. 16.1 months; p = 0.038, respectively). The kinase insert domain receptor rs1531289 G/G genotype tended to be associated with improved PFS (8.0 vs. 7.1 months; p = 0.069). In haplotype analysis, the FLT1 rs9513070/rs9554320/rs9582036 GCA haplotype was associated with inferior PFS and OS (p = 0.004 and p = 0.041, respectively). Conclusion: The VEGFA rs833061 SNP is associated with the ORR, and the FLT1 rs9513070 SNP and FLT1 GCA haplotypes are associated with PFS and OS in advanced CRC patients treated with cytotoxic chemotherapy plus bevacizumab. © 2014 S. Karger AG, Basel.
    Oncology 08/2014; 87(5):280-292. · 2.17 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The kidney is an important site of xenobiotic-induced toxicity. Because the traditional markers of renal injury indicate only severe renal damage, new biomarkers are needed for a more sensitive and reliable evaluation of renal toxicity. This study was designed to identify in vitro noninvasive biomarkers for efficient assessment of nephrotoxicity by using cisplatin as a model of nephrotoxic compounds. To this end, a comparative proteomic analysis of conditioned media from HK-2 human kidney epithelial cells treated with cisplatin was performed. Here, we identified pyruvate kinase M1/M2 isoform M2 (PKM2) and eukaryotic translation elongation factor 1 gamma (EF-1γ) as potential biomarker candidates for evaluation of nephrotoxicity. PKM2 and EF-1γ were increased by cisplatin in a kidney cell-specific manner, most likely due to cisplatin-induced apoptosis. The increase of PKM2 and EF-1γ levels in conditioned media was also observed in the presence of other nephrotoxic agents with different cytotoxic mechanisms such as CdCl2, HgCl2, and cyclosporine A. Rats treated with cisplatin, CdCl2, or HgCl2 presented increased levels of PKM2 and EF-1γ in the urine and kidney tissue. Taken together, this study identified two noninvasive biomarker candidates, PKM2 and EF-1γ, by comparative proteomic analysis. These new biomarkers may offer an alternative to traditional renal markers for efficient evaluation of nephrotoxicity.
    Toxicological sciences : an official journal of the Society of Toxicology. 06/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: UVA is responsible for numerous biological effects on the skin, including premature aging characterized by wrinkles, leathery texture, and mottled pigmentation. The objective of this study was evaluating the protective effect of ginseng leaf extract prepared by Ultraflo L on skin from photodamage. Anti-wrinkle effect of ginseng leaf extract with or without Ultraflo L treatment were tested on human keratinocyte cells (HaCaT) irradiated with ultraviolet (UV) A. Ginseng leaves inhibited ROS generation, GHS depletion, and expression of MMP-2 and MMP-9 induced by UVA irradiation. The glutathione (GSH) content of the cells was significantly increased by over 25 μg mL(-1) of Ultraflo-treated extract (UTGL) as well as by over 100 μg mL(-1) of nonenzyme-treated extract (NEGL) compared to control. UTGL and NEGL treatments significantly decreased expression of metalloproteinase (MMP)-2 and 9 compared with control, but inhibitory effects of two groups on expression of MMPs were not significantly different. Overall, ULtraflo L-treated ginseng leaves inhibited ROS generation, GHS depletion, and expression of MMP-2 and MMP-9 in UVA photodamaged HaCat cells. From these results, enzyme-treated ginseng leaf extract has advantages over untreated ginseng leaves and have potential as a skin protective ingredient against UVA-induced photodamage.
    Applied biochemistry and biotechnology 04/2014; · 1.94 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: No previous studies have investigated the relationship between various anti-ganglioside antibodies and the clinical characteristics of Guillain-Barré syndrome (GBS) in Korea. The aim of this study was to determine the prevalence and types of anti-ganglioside antibodies in Korean GBS patients, and to identify their clinical significance. Serum was collected from patients during the acute phase of GBS at 20 university-based hospitals in Korea. The clinical and laboratory findings were reviewed and compared with the detected types of anti-ganglioside antibody. Among 119 patients, 60 were positive for immunoglobulin G (IgG) or immunoglobulin M antibodies against any type of ganglioside (50%). The most frequent type was IgG anti-GM1 antibody (47%), followed by IgG anti-GT1a (38%), IgG anti-GD1a (25%), and IgG anti-GQ1b (8%) antibodies. Anti-GM1-antibody positivity was strongly correlated with the presence of preceding gastrointestinal infection, absence of sensory symptoms or signs, and absence of cranial nerve involvement. Patients with anti-GD1a antibody were younger, predominantly male, and had more facial nerve involvement than the antibody-negative group. Anti-GT1a-antibody positivity was more frequently associated with bulbar weakness and was highly associated with ophthalmoplegia when coupled with the coexisting anti-GQ1b antibody. Despite the presence of clinical features of acute motor axonal neuropathy (AMAN), 68% of anti-GM1- or anti-GD1a-antibody-positive cases of GBS were diagnosed with acute inflammatory demyelinating polyradiculoneuropathy (AIDP) by a single electrophysiological study. Anti-ganglioside antibodies were frequently found in the serum of Korean GBS patients, and each antibody was correlated strongly with the various clinical manifestations. Nevertheless, without an anti-ganglioside antibody assay, in Korea AMAN is frequently misdiagnosed as AIDP by single electrophysiological studies.
    Journal of Clinical Neurology 04/2014; 10(2):94-100. · 1.89 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Longer needle and complicated insulin injection technique such as injecting at a 45-degree angle and making skinfolds may decrease patient compliance to insulin injection therapy. In this light, shorter insulin needles have been recently developed. However, it is necessary to ascertain that such shorter needles are appropriate for Korean patients with diabetes as well.
    Diabetes & metabolism journal 04/2014; 38(2):120-33.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the treatment outcomes of local excision following preoperative chemoradiotherapy in patients with locally advanced rectal cancer who have not undergone radical surgery for any reason.
    Cancer research and treatment : official journal of Korean Cancer Association. 04/2014; 46(2):158-64.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective Early detection and endoscopic removal of metachronous neoplasm is an important preventive strategy, especially for patients with colorectal cancer (CRC). We aimed to determine risk factors for metachronous colorectal neoplasms developing after curative resection of CRC.Methods We retrospectively reviewed clinical data for patients who underwent curative resection for CRC at the National Cancer Center, Korea, between July 2004 and July 2007. We analyzed the associations of risk factors with metachronous colorectal neoplasms.ResultsA total of 1049 patients were included in this study (647 men and 402 females). Follow-up colonoscopy showed that 454 (43.3%) patients had developed metachronous neoplasms, including 46 (4.3%) patients with advanced adenoma or cancer. Univariate analyses revealed that age >60 years (old age), male gender, diabetes mellitus, hypertension, synchronous adenoma, synchronous multiple adenoma, and synchronous advanced adenoma were associated with the development of metachronous adenoma. Baseline risk factors associated with metachronous advanced neoplasm were old age, synchronous multiple adenoma, and synchronous advanced adenoma. Multivariate analysis showed that old age, synchronous adenoma, and diabetes mellitus were risk factors for the development of metachronous neoplasms. The cumulative incidence of metachronous neoplasms was higher in patients with these risk factors than in those without.Conclusions Old age, synchronous adenoma and diabetes mellitus were risk factors for metachronous adenoma. Therefore, careful surveillance colonoscopy is necessary in these patients.
    Journal of Digestive Diseases 04/2014; · 1.85 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we performed 2-dimensional electrophoresis with protein extracts from the lizard tails, and analyzed the protein expression profiles during the tissue regeneration to identify the dedifferentiation factor. As a result, we identified 18 protein spots among total of 292 spots, of which proteins expression were specifically expressed during blastema formation. We selected lactoferrin as a candidate because it is the mammalian homologue of leech-derived tryptase inhibitor, which showed the highest frequency among the 18 proteins. Lactoferrin was specifically expressed in various stem cell lines, and enhanced the efficiency of iPSC generation upto approximately 7-fold relative to the control. Furthermore, lactoferrin increased the efficiency by 2-fold without enforced expression of Klf4. These results suggest that lactoferrin may induce dedifferentiation at least partly by increasing the expression of Klf4.
    Journal of Microbiology and Biotechnology 03/2014; · 1.40 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Breast cancer is the most common type of cancer in women in many areas and is increasing found in developing countries, where the majority of cases are diagnosed in late stages. Retinoic acids, through their associated nuclear receptors, exert intoxicating effects on cell growth, differentiation and apoptosis, and hold significant promise in relation to cancer therapy and chemoprevention. To enhance our understanding of the molecular mechanisms associated with retinoic acids in the breast cancer cell line MCF-7 in a time-dependent manner, we conducted a proteomic analysis of MCF-7 cells using the 2-DE couple with high-throughput mass spectrometry and bioinformatics tools. In the 2-DE patterns of MCF-7 cells treated with retinoic acid in a time-dependent manner, 35 protein spots were found to be differentially expressed. These were 17 increased, 4 decreased, and 14 unevenly expressed protein spots, all of which were analyzed using LTQ-FTICR mass spectrometry. Furthermore, five candidate proteins, up-regulated, were validated by western blotting. These were nucleoredoxin, latexin, aminomethyltransferase, translationally controlled one tumor protein, and rab GDP dissociation inhibitor β. These observations represent novel findings leading to new insight into the exact mechanism behind the effect of retinoic acids in MCF-7 cells while also identifying possible therapeutic targets for breast cancer diagnosis and novel drug development paths for the treatment of this disease.
    Molecular Biology Reports 03/2014; · 2.51 Impact Factor

Publication Stats

3k Citations
1,026.96 Total Impact Points


  • 2014
    • Samsung Medical Center
      Sŏul, Seoul, South Korea
  • 2012–2014
    • Duksung Women's University
      • College of Pharmacy
      Sŏul, Seoul, South Korea
    • Samsung Advanced Institute of Technology
      Usan-ri, Gyeonggi Province, South Korea
    • Inje University
      Kŭmhae, South Gyeongsang, South Korea
  • 2011–2014
    • Sungkyunkwan University
      • • Samsung Medical Center
      • • Department of Genetic Engineering
      Sŏul, Seoul, South Korea
    • Hankyong National University
      • Department of Food and Biotechnology
      Anseong, Gyeonggi, South Korea
    • Pusan National University
      • Department of Earth Science Education
      Tsau-liang-hai, Busan, South Korea
    • University of Wisconsin–Madison
      Madison, Wisconsin, United States
    • Konkuk University Medical Center
      Changnyeong, South Gyeongsang, South Korea
    • Kyungsung University
      • College of Pharmacy
      Pusan, Busan, South Korea
  • 2009–2014
    • National Cancer Center Korea
      • Colorectal Cancer Branch
      Kōyō, Gyeonggi Province, South Korea
    • The Ohio State University
      • School of Teaching and Learning
      Columbus, Ohio, United States
    • Yanbian University
      Yang-chi-t'eng, Jilin Sheng, China
  • 2013
    • Korea National University of Transportation
      • Department of Computer Engineering
      Sŏul, Seoul, South Korea
    • Indiana University Bloomington
      Bloomington, Indiana, United States
  • 2011–2013
    • Yonsei University Hospital
      • Department of Internal Medicine
      Sŏul, Seoul, South Korea
    • Ulsan University Hospital
      Urusan, Ulsan, South Korea
  • 2009–2013
    • Inha University
      • • Department of Neurosurgery
      • • Department of Biology and Oceanography
      Seoul, Seoul, South Korea
  • 2008–2013
    • Inje University Paik Hospital
      • Department of Internal Medicine
      Goyang, Gyeonggi, South Korea
      Seikan-ri, South Chungcheong, South Korea
    • Wayne State University
      • Department of Pharmaceutical Sciences
      Detroit, MI, United States
    • Korea Electrotechnology Research Institute-KERI
      • Advanced Medical Device Research Center
      Tsau-liang-hai, Busan, South Korea
  • 2007–2013
    • Chungnam National University Hospital
      Sŏul, Seoul, South Korea
  • 2003–2013
    • Asan Medical Center
      • • Asan Institute of Life Sciences
      • • Department of Pulmonary and Critical Care Medicine
      • • Department of Gastroenterology
      Seoul, Seoul, South Korea
  • 2000–2013
    • Catholic University of Korea
      • • College of Medicine
      • • Department of Pediatrics
      Sŏul, Seoul, South Korea
    • Myongji University
      • Department of Chemical Engineering
      Seoul, Seoul, South Korea
  • 2009–2012
    • Sookmyung Women's University
      • Department of Biological Science
      Seoul, Seoul, South Korea
  • 2008–2012
    • Seoul National University Hospital
      • • Department of Pathology
      • • Department of Internal Medicine
      Seoul, Seoul, South Korea
  • 2007–2012
    • University of Regina
      • Department of Chemistry and Biochemistry
      Regina, Saskatchewan, Canada
    • Korea University
      • • Department of Food and Nutrition
      • • Department of Chemistry
      Seoul, Seoul, South Korea
    • Seoul National University
      • • College of Natural Sciences
      • • College of Pharmacy
      • • Department of Chemistry
      Sŏul, Seoul, South Korea
  • 2004–2012
    • Gyeongsang National University
      • • Division of Applied Life Science
      • • Department of Biochemistry
      Chinju, South Gyeongsang, South Korea
  • 2010–2011
    • University of California, Davis
      • J.D. Wheat Veterinary Orthopedic Laboratory
      Davis, CA, United States
    • Seoul National University Bundang Hospital
      • Department of Surgery
      Seoul, Seoul, South Korea
    • Kongju National University
      • Division of Chemical Engineering
      Gongju, South Chungcheong, South Korea
    • Hanyang University Medical Center
      Sŏul, Seoul, South Korea
    • CUNY Graduate Center
      New York City, New York, United States
    • RML Specialty Hospital
      Hinsdale, Illinois, United States
    • Dongguk University
      • Department of Chemistry
      Seoul, Seoul, South Korea
  • 2006–2011
    • Chonbuk National University Hospital
      Sŏul, Seoul, South Korea
    • Stanford Medicine
      • Department of Chemical and Systems Biology
      Stanford, California, United States
  • 2005–2011
    • Stanford University
      • Department of Chemical and Systems Biology
      Stanford, CA, United States
    • Kangwon National University
      • Department of Mathematics
      Gangneung, Gangwon, South Korea
  • 2008–2010
    • Kyung Hee University
      • • Department of Chemistry
      • • Department of Information Display
      • • Advanced Display Research Center
      Seoul, Seoul, South Korea
  • 2007–2010
    • University of Seoul
      • Department of Chemical Engineering
      Sŏul, Seoul, South Korea
  • 2001–2010
    • Hanyang University
      • • College of Medicine
      • • Division of Materials Science and Engineering (MSE)
      Ansan, Gyeonggi, South Korea
  • 2007–2009
    • Yungjin Pharm Co., Ltd.
      Sŏul, Seoul, South Korea
  • 1999–2009
    • Korea Research Institute of Chemical Technology
      • Division of Drug Discovery Research
      Daiden, Daejeon, South Korea
    • University of Ulsan
      • Department of Chemical Engineering
      Urusan, Ulsan, South Korea
  • 2007–2008
    • Chonbuk National University
      • School of Medicine
      Seoul, Seoul, South Korea
  • 2002–2006
    • Ewha Womans University
      • • Center for Cell Signaling Research (CCSR)
      • • College of Pharmacy
      Sŏul, Seoul, South Korea
  • 1993–2003
    • Sogang University
      • Department of Chemical and Biomolecular Engineering
      Sŏul, Seoul, South Korea