Shu Zhang

Second Military Medical University, Shanghai, Shanghai, Shanghai Shi, China

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Publications (4)9.36 Total impact

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    ABSTRACT: A novel annexin gene was isolated from the Taenia solium cDNA library by degenerate PCR. The purified protein was generated by hydrolysis with thrombin in glutathione S-transferase (GST) affinity chromatography following expression in GST tagged pGEX-5T vector. It shares the common structural features of the annexin family and specially possesses two unique fragments between repeating domains II and III which do not exist in other annexin family members revealed by aligning and homology modeling, hence it was designated as Tso ANXB2 according to the new nomenclature of annexins. According to the parasitic behaviors of the origin, a series of coagulation assays was performed, indicating that Tso ANXB2 inhibits extrinsic blood coagulation pathway and platelet aggregation also has platelet binding activity. Nevertheless, the mutant protein deleting the consensus coagulation-related KGD motif of Tso ANXB2 showed significant decreasing platelet binding and anticoagulation activity.
    Acta Tropica 11/2006; 99(2-3):165-72. DOI:10.1016/j.actatropica.2006.07.006 · 2.27 Impact Factor
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    ABSTRACT: The development and widespread use of DNA-based vaccination against infectious pathogens have been a great triumph of medical science. Quality control of DNA vaccines as biopharmaceutical productions is a problem to solve. Residual genomic DNA of engineering bacteria has been identified as a potential risk factor, so whose level must be controlled under the regulatory standards. We report a dot-blot hybridization method to detect residual host cell DNA in purified DNA vaccines. The assay utilizes PCR amplified and digoxigenin-labeled Escherichia coli 16S rRNA gene as probe. The sensitivity of the dot-blot hybridization assay with E. coli 16S rRNA gene probe was evaluated in comparison with single copy UidR gene probe. The optimized dot-blot hybridization assay had both low background and a suitable sensitivity, detecting 10 pg of residual E. coli DNA. The method is suitable in the routine use of measuring the levels of residual E. coli DNA in the pharmaceutical-grade DNA vaccine.
    Vaccine 04/2006; 24(14):2656-61. DOI:10.1016/j.vaccine.2005.11.066 · 3.62 Impact Factor
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    ABSTRACT: The S2 domain of the severe acute respiratory syndrome coronavirus (SARS-CoV) spike (S) protein is responsible for fusion between virus and target cell membranes, and is expected to be immungenic. In this study, we investigated the immune responses against the S2 subunit in BALB/c mice, which were vaccinated either with plasmid DNA encoding the S2 domain (residues 681-1120), the recombinant S2 fragment (residues 681-980) in incomplete Freund's adjuvant, or with inactivated SARS-CoV. The increased number of specific cytotoxic cells (CTLs) and the high titer of specific antibody showed stimulation of both arms of the immune system in these groups. The shift in cytokines suggested that Th1-polarized immune response was induced by plasmid pCoVS2, meanwhile the Th2-dominant response was induced by recombinant S2 fragment and inactivated vaccine. However, the titer of neutralizing antibodies was only detectable in mice immunized with inactivated virus, but not with pCoVS2 plasmid. Taken together, the S2 domain could induce specific cellular immune response and a high level of total IgG but little neutralizing antibodies against infection by SARSCoV.
    DNA and Cell Biology 09/2005; 24(8):510-5. DOI:10.1089/dna.2005.24.510 · 2.06 Impact Factor
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    ABSTRACT: Annexin B1 has been cloned in Escherichia coli (E. coli) K802 in previous works. After temperature shift induction the protein was expressed as soluble form. Then a separation method for annexin B1 from E. coli cells by ion-exchange chromatography has been developed and validated. The method exhibited a good reproducibility and is easy to operate. It has shown that annexin B1 isolated by chromatography had high anticoagulant activity.
    Chromatographia 11/2003; 58(11):713-716. DOI:10.1365/s10337-003-0107-6 · 1.41 Impact Factor