Shuo Zhang

Shanghai Institute of Applied Physics, Shanghai, Shanghai Shi, China

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Publications (50)224.02 Total impact

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    ABSTRACT: Cell cycle processes play a vital role in vascular endothelial proliferation and dysfunction. Cell division cycle protein 14 (Cdc14) is an important cell cycle regulatory phosphatase. Previous studies in budding yeast demonstrated that Cdc14 could trigger the inactivation of mitotic cyclin-dependent kinases (Cdks), which are required for mitotic exit and cytokinesis. However, the exact function of human Cdc14 (hCdc14) in cell cycle regulation during vascular diseases is yet to be elucidated. There are two HCdc14 homologs: hCdc14A and hCdc14B. In the current study, we investigated the potential role of hCdc14A in high glucose-, free fatty acids (FFAs)-, and hypoxia-induced injury in cultured human brain vascular endothelial cells (HBVECs). Data revealed that high glucose, FFA, and hypoxia down-regulated hCdc14A expression remarkably, and also affected the expression of other cell cycle-related proteins such as cyclin B, cyclin D, cyclin E, and p53. Furthermore, the combined addition of the three stimuli largely blocked cell cycle progression, decreased cell proliferation, and increased apoptosis. We also determined that hCdc14A was localized mainly to centrosomes during interphase and spindles during mitosis using confocal microscopy, and that it could affect the expression of other cycle-related proteins. More importantly, the overexpression of hCdc14A accelerated cell cycle progression, enhanced cell proliferation, and promoted neoplastic transformation, whereas the knockdown of hCdc14A using small interfering RNA produced the opposite effects. Therefore, these findings provide novel evidence that hCdc14A might be involved in cell cycle regulation in cultured HBVECs during high glucose-, FFA-, and hypoxia-induced injury.
    Cellular Signalling 05/2015; 27(1). DOI:10.1016/j.cellsig.2014.10.003 · 4.47 Impact Factor
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    ABSTRACT: The purpose of this work is to examine the serum proteomic profiles associated with the subsequent development of diabetic nephropathy (DN) in patients with type 2 diabetes and to develop and validate a decision tree based on the profiles to predict the risk of DN in advance by albuminuria. Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry was used to obtain the proteomic profiles from baseline serum samples of 84 patients with type 2 diabetes with normal albuminuria, including 42 case subjects who developed DN after 4 years and 42 control subjects who remained normoalbuminuric over the same 4 years. From signatures of protein mass, a decision tree was established for predicting DN. At baseline, urinary albumin/creatinine ratio was similar between the case and control groups. The intensities of 5 peaks detected by CM10 chips appeared up-regulated, whereas 18 peaks were down-regulated more than twofold in the case group than compared with the control group in the training set. An optimum discriminatory decision tree for case subjects created with four nodes using four distinct masses was challenged with testing set. The positive predictive value was 77.8 % (7/9), and the negative predictive value was 72.7 % (8/11). We developed and validated a decision tree to predict DN in patients with type 2 diabetes.
    Wiener klinische Wochenschrift 05/2015; DOI:10.1007/s00508-014-0679-1 · 0.79 Impact Factor
  • PLoS ONE 04/2015; 10(4):e0120707. DOI:10.1371/journal.pone.0120707 · 3.53 Impact Factor
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    ABSTRACT: Existing methods could not discriminate between inflammation and other diseases, which might occur in hypothalamus, such as neurogliocytoma, germinoma, lymphoma, and so on. Given its location in the brain, it was not practical to obtain tissue using standard surgical methods.We reported the first case of a patient with hypothalamus lesion, who was diagnosed as hypothalamitis by stereotactic biopsy. This precise diagnosis allowed proper medical treatments.We reported a case of a patient with hypothalamus lesion. To confirm the diagnosis, with informed consent from the family, a successful stereotactic hypothalamic biopsy was performed by neurosurgeons.Immunohistochemical results of biopsy specimens from the hypothalamus lesion revealed inflammatory infiltrates, which were composed mainly of lymphocytes, plasma cells, and histiocytes, and were stained with leucocyte common antigen (LCA), κ 1, and cluster of differentiation 18. Final pathological diagnosis was lymphoplasmacytic proliferative, granuloma-like inflammatory pseudotumor, with immunoglobulin G deposition. Based on the pathological diagnosis, we treated the patient with glucocorticoid and azathioprine. Remarkable improvements were observed in both magnetic resonance imaging (MRI) and patient's symptoms.Stereotactic biopsy for intracranial lesions was a reliable and relatively safe procedure, even for hypothalamus. It was an effective method with high diagnostic yield. With correct diagnosis, it was much easier to choose correct treatment.
    Medicine 02/2015; 94(5):e447. DOI:10.1097/MD.0000000000000447 · 4.87 Impact Factor
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    ABSTRACT: To tailor the catalytic activities of metal catalysts at will to achieve efficient conversion in chemical processes remains a challenge, particularly for noble metals, such as Pt. We demonstrate herein that encapsulation within the carbon nanotube (CNT) channels with a diameter of 1.0−1.5 nm not only allows restriction of the size of Pt nanoclusters around 1.0 nm but also enables modulating of the Pt species at the active reduced states through host−guest interaction. The encapsulated Pt is protected from oxygen under reaction conditions in toluene oxidation up to 200 °C, as unveiled by in situ X-ray absorption spectroscopy and density functional theory calculations. As a result, the encapsulated Pt clusters deliver a remarkably higher activity and stability than the clusters located on the open surfaces of the CNT exterior walls and carbon black support, although the latter are much more accessible to reactants. This characteristic of the CNT channels can be explored to tune the properties of other metal catalysts for oxidation reactions.
    ACS Catalysis 01/2015; DOI:10.1021/cs501763k · 7.57 Impact Factor
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    ABSTRACT: A series of ceria supported perovskite-based lean-burn NOx trap (LNT) catalysts 10 wt.% LaCoO3/xK2CO3/CeO2 (denoted as L/xK/C, x = 1, 3, 5, 8 wt.%) were prepared by successive impregnation. They display excellent performance for NOx storage and reduction, especially the one with 3% K2CO3. The formation of supported perovskite LaCoO3 on CeO2 is confirmed by XAFS characterization. The results of XPS and O2-TPD reveal that the supported perovskite LaCoO3 contains considerable amounts of surface adsorbed oxygen, which are responsible for NO to NO2 oxidation during NOx storage. The catalyst L/3K/C exhibits fast and complete NOx trapping in lean condition, showing little NOx leak for long time, over which a particularly high NOx reduction percentage (97.7%) and NOx to N2 selectivity (98.5%) are simultaneously achieved in cyclic lean/rich atmospheres. FT-IR and CO2-TPD results demonstrate the diversity of K species including –OK groups, dispersed surface K2CO3 species and bulk or bulk-like K2CO3 species. The dispersion and states of K species show big impact on NOx storage pathways. In situ DRIFTS results indicate that on L/xK/C NOx is stored as diverse nitrates without evident nitrite species detected during NOx adsorption and storage, which further verifies their excellent oxidation ability.
    Chemical Engineering Journal 01/2015; 260:357–367. DOI:10.1016/j.cej.2014.09.016 · 4.06 Impact Factor
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    ABSTRACT: Zipper‑interacting protein kinase (ZIPK) is a novel serine/threonine protein kinase and a member of a large family of protein kinases, known as the death‑associated protein kinases. However, the function of ZIPK has yet to be fully elucidated, as few physiological substrates have currently been identified. In the present study, a yeast two‑hybrid screen was used and the human cell division cycle 14A (HsCdc14A) phosphatase was identified as a novel ZIPK binding protein. To the best of our knowledge, this is the first study to report the interaction between these proteins. The interaction between ZIPK and HsCdc14A was confirmed by in vitro experiments. In addition, ZIPK‑mediated phosphorylation was shown to activate the phosphatase activity of HsCdc14A. These findings indicated that ZIPK may also be involved in the regulation of the cell cycle in human cells, by interacting with HsCdc14A.
    Molecular Medicine Reports 12/2014; 11(4). DOI:10.3892/mmr.2014.3067 · 1.48 Impact Factor
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    ABSTRACT: Cu2 MoS4 nanosheets are synthesized by a solvothermal method in which the Cu2 O starting material acts as a sacrificial template. The microstructure of the Cu2 MoS4 nanosheets is characterized at the atomic level, and the growth mechanism is monitored at the nanoscale through systematic time-dependent experiments. As a result, the unprecedented observation of the allotropic phase change in Cu2 MoS4 that occurs during the solvothermal process is possible.
    Small 11/2014; 10(22):4637–4644. DOI:10.1002/smll.201400752 · 7.51 Impact Factor
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    ABSTRACT: Transition-metal-doped Bi2Se3 crystals, X0.05Bi2Se3 (X = Cr, Co, Ni, and Cu), are prepared and studied by powder x-ray diffraction and extended x-ray absorption fine structure (EXAFS) measurements to get the local structures around the dopants. Cr atoms substitute Bi atoms and Co atoms may substitute Bi atoms or form Co3Se4 (C2/m) clusters. Cu is also found taking the Bi substitutional site, which differs from the reported superconductor phase of Cu-doped Bi2Se3, suggesting the dependence of site occupancy of Cu in Bi2Se3 on the process of sample growing. For Ni0.05Bi2Se3, Ni atoms form Ni3Se4 (C2/m) clusters. The nearest neighbors of X (X = Cr, Co, and Cu) are Se atoms, and the X-Se bond lengths are extracted from EXAFS as 2.50 angstrom for Cr-Se, 2.40 angstrom for Co-Se, and 2.38 angstrom for Cu-Se, which show the direct evidences of dramatic structural relaxations around 3d dopants. The bond information of local structures around dopants is valuable for subsequent theoretical studies, and can hardly be obtained from other techniques.
    Physical review. B, Condensed matter 09/2014; 90(9):094107. DOI:10.1103/PhysRevB.90.094107 · 3.66 Impact Factor
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    ABSTRACT: Prolactin may reduce false-negative results in diagnosing Cushing's disease (CD) during inferior petrosal sinus sampling (IPSS). Prolactin normalization could improve the accuracy of IPSS in predicting adenoma lateralization in CD. However, none of the previous studies had involved the use of desmopressin during IPSS. Our objective was to examine the utility of prolactin measurement during IPSS with desmopressin stimulation. We conducted a retrospective analysis of 40 patients (including 31 females) with ACTH-dependent Cushing's syndrome who underwent IPSS between 2010 and 2013. Thirty-eight CD patients were partitioned into true positive (n = 35) and false negative (n = 3). The proportion of improper IPSS venous sampling defined by corresponding IPS:P (inferior petrosal sinus to peripheral) prolactin ratio <1.8 was significantly different between two groups (P = 0.004). Applying a prolactin-normalized ACTH IPS:P ratio >0.8 cutoff could increase the sensitivity of IPSS to 38/38 (100 %). Among the 31 patients with histopathologically proven adenoma localization, correct prediction of adenoma lateralization was obtained in 14/31 (45 %) patients by a peak intersinus ACTH gradient of ≥1.4 in baseline and was not improved by desmopressin stimulation. Left-right intersinus gradients of unilateral prolactin-adjusted ACTH IPS:P ratios could increase the correct prediction of adenoma lateralization to 20/31 (65 %) in baseline and 24/31 (77 %) (P = 0.006) after desmopressin stimulation, respectively. Prolactin is helpful to adjust negative results of IPSS with desmopressin stimulation. It may improve the accuracy in predicting adenoma lateralization in CD as well.
    Endocrine 07/2014; 48(2). DOI:10.1007/s12020-014-0338-3 · 3.53 Impact Factor
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    ABSTRACT: We report an alternative mechanism for the optical response of an iodine-modified anatase TiO2 photocatalyst material. Unlike the general realization that the iodine atom provides impurity levels within the optical band gap, we suggest that a distorted surface structure plays a dominant role. Anatase pure TiO2 and iodine-modified TiO2 (I-TiO2) nanocrystals were synthesized by a simple solvothermal method, and I-TiO2 exhibited an extended absorption edge up to 550 nm. Employing iodine K-edge X-ray absorption fine structure (XAFS), we demonstrate that iodine is not incorporated into the nanoparticle interior but exists in the form of IO3– groups at the surface. Furthermore, this IO3– cluster adsorption largely induces a disordered structure, as revealed by Ti 2p3/2 X-ray photoelectron spectroscopy, high-resolution transmission electron microscopy, and Ti K-edge XAFS data fitting. Density functional theory calculation indicates that this distorted structure can form a midgap state, which should be responsible for its excellent optical property. This finding represents a promising route for improving the optical response of the nanophotocatalyst system via surface treatment.
    The Journal of Physical Chemistry C 06/2014; 118(25):13726–13732. DOI:10.1021/jp503966r · 4.84 Impact Factor
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    ABSTRACT: The efficient use of natural gas will require catalysts that can activate the first C-H bond of methane while suppressing complete dehydrogenation and avoiding overoxidation. We report that single iron sites embedded in a silica matrix enable direct, nonoxidative conversion of methane, exclusively to ethylene and aromatics. The reaction is initiated by catalytic generation of methyl radicals, followed by a series of gas-phase reactions. The absence of adjacent iron sites prevents catalytic C-C coupling, further oligomerization, and hence, coke deposition. At 1363 kelvin, methane conversion reached a maximum at 48.1% and ethylene selectivity peaked at 48.4%, whereas the total hydrocarbon selectivity exceeded 99%, representing an atom-economical transformation process of methane. The lattice-confined single iron sites delivered stable performance, with no deactivation observed during a 60-hour test.
    Science 05/2014; 344(6184):616-9. DOI:10.1126/science.1253150 · 31.48 Impact Factor
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    ABSTRACT: A non-platinic lean NOx trap catalyst MnOx-K2CO3/K2Ti8O17 (K2CO3 loading: 25 wt %) was prepared via successive impregnation, which exhibits a large NOx storage capacity (3.21 mmol/g), a high NOx reduction percentage (98.5%) and an ultralow selectivity of NOx to N2O (0.3%). The catalyst was characterized by multiple techniques including XRD, SEM/HRTEM, EXAFS, FT-IR, CO2-TPD and in situ DRIFTS. Except for K2O, -OK groups, surface K2CO3 and bulk or bulk-like K2CO3, an unknown titanate phase with a K/Ti atomic ratio higher than 2/8 is identified, which is also active and regenerative for NOx storage and reduction. In-situ DRIFTS results reveal that NOx is mainly stored as bidentate nitrates and bidentate nitrite species in the catalyst. The appearance of negative bands around 1555 and 1575 cm–1 (C═O stretching vibration in bidentate carbonates) suggests the involvement of carbonates in NOx storage. Based upon the characterization results, a carbonate-involved NOx storage/reduction mechanism was proposed.
    Industrial & Engineering Chemistry Research 05/2014; 53(20):8416–8425. DOI:10.1021/ie501034u · 2.24 Impact Factor
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    ABSTRACT: We found a direct evidence for the existence of the local chemical Bi–In bonds in the BiIn2 melt. These bonds are strong and prevail, dominating the structure evolution of the intermetallic clusters. From the local structure of the melt-quenched BiIn2 ribbon, the chemical Bi–In bonds strengthen compared with those in the equilibrium solidified alloy. The chemical bonds in BiIn2 melt remain solid during a rapid quenching process. The results suggest that the intermetallic clusters in the melt evolve into the as-quenched intermetallic phase, and the intermetallic phase originates from the chemical bonds between unlike atoms in the melt. The chemical bonds preserve the chemical ordered clusters and dominate the clusters evolution.
    Physics Letters A 05/2014; 378(24-25). DOI:10.1016/j.physleta.2014.04.037 · 1.63 Impact Factor
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    ABSTRACT: Biologic abnormalities in vascular smooth muscle cells (VSMC) may perform a crucial role in the pathogenesis of diabetic vascular disease. The principal aim of this study was to determine the effects of zipper‑interacting protein kinase (ZIPK) on human aortic smooth muscle cells (HASMCs) stimulated by high glucose (HG). To elucidate the role of ZIPK in HG‑treated HASMCs, we overexpressed ZIPK by lentivirus infection and knocked down ZIPK by gene deletion using ZIPK shRNA. Flow cytometry and Cell Counting kit‑8 (CCK‑8) were separately used to analyze cell apoptosis and proliferation. Migratory activity was examined using transwell migration chamber assays. The results showed that ZIPK overexpression inhibited cell growth and migration, enhanced cell apoptosis, and reversed cell cycle disturbance by regulating the related proteins of cellular physiological process, such as human cell division cycle 14A phosphatase (Hcdc14A) and intercellular adhesion molecule 1 (ICAM‑1). In conclusion, the results suggested that ZIPK plays a role in HG‑treated HASMCs, indicating ZIPK is a potential therapeutic target for the treatment of diabetic vascular complications.
    International Journal of Molecular Medicine 03/2014; 33(5). DOI:10.3892/ijmm.2014.1697 · 1.88 Impact Factor
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    ABSTRACT: Two CoSiB metallic glasses of low Co contents, which consist of different clusters, have recently been developed by addition of solute atoms. In this work, the atomic structure and the magnetic properties of the two CoBSi metallic glasses were elucidated by state-of-the-art extended X-ray absorption fine structure spectroscopy (EXAFS) combining with ab initio molecular-dynamics (AIMD) computational techniques. Besides, the origin of these magnetic behaviors was discussed in view of the EXAFS results and atomic structures of the metallic glasses.
    Journal of Magnetism and Magnetic Materials 02/2014; 352:49-55. DOI:10.1016/j.jmmm.2013.10.011 · 2.00 Impact Factor
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    ABSTRACT: Local structure inheritance signatures were observed during the alloying process of the Ag50Sn50 melt, using high-temperature X-ray diffraction and ab initio molecular dynamics simulations. The coordination number Nm around Ag atom is similar in the alloy and in pure Ag melts (Nm ∼ 10), while, during the alloying process, the local structure around Sn atoms rearranges. Sn-Sn covalent bonds were substituted by Ag-Sn chemical bonds, and the total coordination number around Sn increases by about 70% as compared with those in the pure Sn melt. Changes in the electronic structure of the alloy have been studied by Ag and Sn K-edge X-ray absorption spectroscopy, as well as by calculations of the partial density of states. We propose that a leading mechanism for local structure inheritance in Ag50Sn50 is due to s-p dehybridization of Sn and to the interplay between Sn-s and Ag-d electrons.
    Journal of Applied Physics 01/2014; 115(4):043506-043506-5. DOI:10.1063/1.4863125 · 2.19 Impact Factor
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    ABSTRACT: Several novel circulating adipokines are associated with insulin resistance and inflammation. Little information exists in NAFLD about three recently recognized adipokines lipocalin-2, cathepsin S and chemerin. To assess the relationship between serum lipocalin-2, cathepsin S and chemerin levels and the development of non-alcoholic fatty liver in Chinese subjects, we measured serum lipocalin-2, cathepsin S and chemerin levels in 903 Chinese subjects by ELISA. Among the study population, 436 patients are with B-mode ultrasound-proven NAFLD and 467 controls. Levels of lipocalin-2, but not cathepsin S and chemerin, were significantly elevated in NAFLD versus control [lipocalin-2, 89.67 ± 4.47 vs. 68.70 ± 3.65 ng/mL (p < 0.001)]. After stepwise linear regression analysis adjusting for potential cofounders, further revealed that serum lipocalcin-2 was an independent predictor of NAFLD in whole cohort (standardized β = 0.114, t = 2.347, p = 0.02). Lipocalin-2 levels correlated with insulin resistance (homeostasis model assessment of insulin resistance) and inflammation (CRP) in whole cohorts and NAFLD, whereas cathepsin S and chemerin only correlated positively with insulin resistance and inflammation in whole cohorts. Our results indicated that circulating lipocalin-2, produced by adipocytes, are elevated and may contribute to the development of NAFLD. Serum lipocalin-2, which correlates with inflammation and insulin resistance, may have a direct pathogenic link to disease progression.
    Molecular Biology Reports 01/2014; 41(3). DOI:10.1007/s11033-013-2977-5 · 1.96 Impact Factor
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    ABSTRACT: There are 2 different types of fat present in mammals: white adipose tissue, the primary site of energy storage, and brown adipose tissue, which is specialized in energy expenditure. Factors that specify the developmental fate and function of brown fat are poorly understood. Bone morphogenic proteins (BMPs) play an important role in adipogenesis. While BMP4 is capable of triggering commitment of stem cells to the white adipocyte lineage, BMP7 triggers commitment of progenitor cells to a brown adipocyte lineage and activates brown adipogenesis. To investigate the differential effects of BMPs on the development of adipocytes, C3H10T1/2 pluripotent cells were pretreated with BMP4 and BMP7, followed by different adipogenic induction cocktails. Both BMP4 and BMP7 unexpectedly activated a full program of brown adipogenesis, including induction of the brown-fat-defining marker uncoupling protein 1 (UCP1), increasing the expression of early regulators of brown fat fate PRDM16 (PR-domain-containing 16), and induction of mitochondrial biogenesis and function. Implantation of BMP4-pretreated C3H10T1/2 cells into nude mice resulted in the development of adipose tissue depots containing UCP1-positive brown adipocytes. Interestingly, BMP4 could also induce brown fat-like adipocytes in both white and brown preadipocytes, thereby decreasing the classical brown adipocyte marker, Zic1, and increasing the recently identified beige adipocyte marker, TMEM26. The data indicate an important role of BMP4 in promoting brown adipocyte differentiation and thermogenesis in vivo and in vitro, and offers a potentially new therapeutic approach for the treatment of obesity.
    AJP Endocrinology and Metabolism 12/2013; 306(4). DOI:10.1152/ajpendo.00119.2013 · 4.09 Impact Factor
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    ABSTRACT: Previous studies have shown active brown adipose tissue is present in adults and may play important roles in regulating energy homeostasis. However, nearly every study was done with patients undergoing scanning for cancer surveillance whose metabolism and BAT activity may not reflect that seen in healthy individuals. The objective of this study was to investigate the prevalence and predictors of active BAT in Chinese adults, particularly in healthy individuals. In this study, a total of 31,088 consecutive subjects with age≥18 years old performed PET/CT scans in the site. We measured BAT activity via 18F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography in these subjects who underwent scanning for either routine medical check-up (MC) or cancer surveillance (CS) in Shanghai. Then we investigated the predictors of active BAT, particularly in healthy individuals. In both groups, the prevalence of BAT was higher in women than men. Using a multivariate logistic analysis, we found in the MC group, age, sex, BMI, and thyroid high glucose uptake were significant predictors of BAT activity. Similarly, in the CS group, age, sex, and BMI were significant predictors of BAT activity, but not thyroid high glucose uptake. In Chinese adults, BAT activity correlates inversely with BMI and thyroid high glucose uptake, which reinforces the central role of brown fat in adult metabolism and clues to a potential means for treating metabolic syndrome.
    European Journal of Endocrinology 11/2013; DOI:10.1530/EJE-13-0712 · 3.69 Impact Factor