S. Soler

Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana, Valenza, Valencia, Spain

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Publications (21)32.73 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Tomato spotted wilt virus (TSWV) causes serious diseases of many economically important crops. Disease control has been achieved by breeding tomato and pepper cultivars with the resistance genes Sw-5 and Tsw, respectively. However, TSWV isolates overcoming these genetic resistances have appeared in several countries. To evaluate the risk of spread of these resistance-breaking isolates, we tested their ability of transmission by the main vector of TSWV, the thrips Frankliniella occidentalis. We compared the transmission rate by thrips of six TSWV isolates of different biotype (able or unable to overcome this resistance in pepper and tomato), and with divergent genotype (A and B). Our results indicate that the transmission rate was related to the amount of virus accumulated in thrips but not to virus accumulation in the source plants on which thrips acquired the virus. No correlation was found between transmission efficiency by thrips and the genotype or between transmission efficiency and the ability of overcoming both resistances. This result suggests that resistance-breaking isolates have the same potential to be transmitted as the isolates unable to infect resistant tomato and pepper cultivars.
    Annals of Applied Biology 11/2013; · 2.15 Impact Factor
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    ABSTRACT: A quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) procedure using a general primer set and three TaqMan(®)MGB probes was developed for general and genotype-specific detection and quantitation of the genomic M segment of Tomato spotted wilt virus (TSWV). Standard curves using RNA transcripts homologous to the three probes allowed reproducible quantitative assays with a wide dynamic range (10(3)-10(10) TSWV M segment RNA copies/ng of total RNA) and high sensitivity. This protocol was assayed with a battery of TSWV isolates, covering the range of the present known genetic variation, in single and/or mix infections in three plant hosts, as well as in the thrips vector Frankliniella occidentalis. This quantitative detection assay will be a valuable tool for molecular biology and epidemiology studies, diagnosis and disease control.
    Journal of virological methods 05/2011; 176(1-2):32-7. · 2.13 Impact Factor
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    ABSTRACT: Viral diseases are a serious limitation to the tomato crop in the region of València, Spain. A survey of tomato viruses in open field cultivation plots was made in the three provinces of this region. A total of 228 plots classified according to the origin of the seed (farmer seed plots or commercial seed plots) were surveyed, from which 1300 individual plants were sampled and tested for Cucumber mosaic virus (CMV), Pepino mosaic virus (PepMV), Parietaria mottle virus (PMoV), Potato virus Y (PVY), Tomato mosaic virus (ToMV), Tomato spotted wilt virus (TSWV) and for the tomato yellow leaf curl disease (TYLCD). Virus infection was detected in 58.9% of the plants sampled and in 86.0% of the plots surveyed. All these viruses were detected, and the most prevalent were ToMV and PVY (34.1% and 27.1% of infected plants, respectively), but PMoV and TYLCD were the less prevalent (1.2% and 1.3% of infected plants, respectively). Differences among provinces and seed origin were found for most of the viruses studied. In particular, both ToMV and PVY had a higher level of infection in plants from farmer seed plots than in commercial seed plots, which accounts for the higher percentage of virus-infected plants in the former (64.2%) when compared to the latter (49.1%). Single and multiple infections were found in 42.38% and 16.54% of the samples, respectively. The most common multiple infection was of ToMV, PVY or both. These results show that the percentage of infected plants and plots in open field cultivation is very high in this region and the origin of the seed is an important factor in the incidence of virus infection. In this respect, preventive measures, including virus-free seed, resistant cultivars and improved cultural practices, could reduce the incidence of virus infection.
    Journal of Phytopathology 11/2010; 158(11‐12):797 - 805. · 1.00 Impact Factor
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    ABSTRACT: Tomato spotted wilt virus (TSWV) causes severe economic losses in many crops worldwide and often overcomes resistant cultivars used for disease control. Comparison of nucleotide and amino acid sequences suggested that tomato resistance conferred by the gene Sw-5 can be overcome by the amino acid substitution C to Y at position 118 (C118Y) or T120N in the TSWV movement protein, NSm. Phylogenetic analysis revealed that substitution C118Y has occurred independently three times in the studied isolates by convergent evolution, whereas the substitution T120N was a unique event. Analysis of rates of non-synonymous and synonymous changes at individual codons showed that substitution C118Y was positively selected.
    Journal of General Virology 09/2010; 92(Pt 1):210-5. · 3.13 Impact Factor
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    ABSTRACT: Resistant Capsicum chinense accessions PI-152225 and PI-159236 and the susceptible cultivar ‘Negral’ of Capsicum annuum were used in three experiments to characterize the resistance to tomato spotted wilt virus (TSWV) in these materials. In the first experiment viral movement in the whole plant was studied at two growth stages (2- and 4-leaf stage). In the second experiment the movement within the inoculated leaf was analysed at three growth stages (2-, 3-, and 4-leaf stage). Two techniques were used in this assay: double antibody sandwich – enzyme-linked immunosorbent assay (DAS–ELISA) and direct tissue blotting (DTB). In the latter experiment viral accumulation in different types of samples was evaluated by DAS–ELISA. The DTB technique showed that viral movement within the inoculated leaf is restricted in the resistant plants. The inoculated area was not totally infected in resistant accessions and slower viral movement within the inoculated area was observed. Detection of weak ELISA positives in inoculated and adjacent areas in resistant plants might not be caused by a lower viral replication, but may result from a low foliar area affected by the virus, as viral movement was restricted in certain areas around the necrotic lesions. However, ELISA absorbance values of the necrotic lesions were similar to the values of infected tissue of ‘Negral’. These observations point to a restricted viral cell-to-cell movement in the resistant accessions.
    Canadian Journal of Plant Pathology 12/2009; December 1999(Vol. 21):317-325. · 1.12 Impact Factor
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    ABSTRACT: The genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/ helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with α-helix and β-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome.
    Virus Genes 08/2009; 39(2):256-60. · 1.84 Impact Factor
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    ABSTRACT: Recombinant DNA technology was used to raise a polyclonal antiserum against the coat protein (CP) of Parietaria mottle virus (PMoV). The CP gene was expressed in Escherichia coli as a fusion to a 6xHis tag and purified by affinity chromatography. Recombinant purified protein was used as antigen to raise a polyclonal antiserum. This polyclonal antiserum consistently detected PMoV specifically infected tomato plants from different commercial tomato crops by indirect enzyme-linked immunosorbent assay (I-ELISA) and direct tissue-printing immunoassay (DTBIA).
    Journal of Phytopathology 02/2009; 157(7‐8):511 - 513. · 1.00 Impact Factor
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    ABSTRACT: A polyprobe for the simultaneous detection by non-isotopic molecular hybridisation has been developed to detect any of the following six viruses causing important economic losses in tomato crops: Tomato spotted wilt virus, Tomato mosaic virus, Pepino mosaic virus, Cucumber mosaic virus, Potato Y virus and Parietaria mottle virus. The polyprobe detected all six viruses with similar sensitivity to that obtained using individual riboprobes. In addition, we evaluated the possible use of the tissue-printing as a sample preparation technique applied to routine diagnosis of tomato plants with the polyprobe.
    European Journal of Plant Pathology 12/2008; 123(1):117-123. · 1.71 Impact Factor
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    ABSTRACT: Ever since the arrival of the tomato to Spain in the 16 th century, great diversification of the crop has taken place, giving rise to a rich collection of varietal types. The 'Comunidad Valenciana', with its deep-rooted agricultural tradition, is one of the Spanish regions with the greatest diversity in traditional tomato varieties, characterised by their local adaptation and high fruit quality. Nevertheless, traditional varieties of tomato have been progressively abandoned over recent decades. A survey was carried out in the 'Huerta de Valencia' area of the 'Comunidad Valenciana' in order to evaluate the factors involved in the genetic erosion of traditional tomato varieties, as a model of the process affecting vegetable crops in Europe. The growth of urban areas that absorb horticultural land, the change in agricultural techniques, the low profitability of farms and their small size, the advanced age of farmers, the conversion of vegetable gardens to other crops and the incidence of viral diseases, have been identified as the major factors affecting genetic erosion in this analysis. The development of resistant traditional varieties through a formal plant breeding programme, and the consolidation of the specialised markets that efficiently exploit the organoleptic quality of these varieties, would help to assure the profitability of these varieties, and hence their active conservation on an on-farm basis. Consequently, the loss of these materials that make up gene combinations of an outstanding value could be prevented, and farmers would obtain a profitable alternative in a highly competitive agriculture.
    International Journal of Plant Production 10/2007; 1. · 1.15 Impact Factor
  • C López, S Soler, F Nuez
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    ABSTRACT: The complete nucleotide sequence of the genomes of two Spanish isolates (LE-2000 and LE-2002) from tomato and one Peruvian isolate (LP-2001) from Lycopersicon peruvianum of the Pepino mosaic virus (PepMV) were determined. The tomato isolates share identities higher than 99%, while the genome of LP-2001 had mean nucleotide identities of 95.6% to 96.0% with tomato isolates. The predicted amino acid sequences showed similarities ranging between 95.2% and 100% with TGBp3 and TGBp2 and CP proteins, respectively. In LP-2001 two main differences were found with respect to the tomato isolates; (i) the 5' untranslated region (UTR) was 2 nt shorter by deletion at position 12-13 and it had some polymorphims at the putative promoter sequence reported for PepMV tomato isolates and other potexviruses, which could be functionally significant for RNA replication, and (ii) the TGBp3 protein had two extra amino acids in the C-terminal region.
    Archives of Virology 04/2005; 150(3):619-27. · 2.28 Impact Factor
  • Agronomy for Sustainable Development - AGRON SUSTAIN DEV. 01/2005; 25(2):237-241.
  • S. Soler, C. López, F. Nuez
    Plant Disease - PLANT DIS. 01/2005; 89(11):1244-1244.
  • Source
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    ABSTRACT: Resistance conferred by the Tsw locus from Capsicum chinense against Tomato spotted wilt virus (TSWV) has been widely used in breeding programmes. Nevertheless, this resistance depends on inoculation conditions, and isolates able to overcome it have already been detected. In this work 29 accessions of several Capsicum species have been mechanically inoculated with TSWV to identify new sources of resistance. Five accessions showed variable percentages of resistant plants, two of which did not show local lesions on inoculated leaves, suggesting that the response was not mediated through hypersensitivity. Two of these accessions also had a remarkable reduced viral accumulation compared to susceptible control. ECU-973., a C. chinense accession, showed the best performance against TSWV, with 100% resistant plants. This response was confirmed after mechanical inoculation with three different TSWV isolates. The resistance was maintained when the accession was inoculated with TSWV using a high pressure of viruliferous thrips. These results open new possibilities in the development of a durable resistance to TSWV in pepper.
    Annals of Applied Biology 09/2003; 143(2):143 - 152. · 2.15 Impact Factor
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    ABSTRACT: Pepino mosaic virus (PepMV), a potexvirus first described in 1980 from pepino (Solanum muricatum) plants cultivated in Peru, was isolated from diseased tomato plants in the Netherlands in 1999, and is now the cause of an emerging tomato disease in Europe. In a survey of central and southern Peru, 65 wild and four cultivated populations of Lycopersicon, as well as six populations of other species of Solanaceae, were tested for the presence of PepMV and six other viruses. Of the Lycopersicon population sampled, 23 (35.4%) reacted positively in double antibody sandwich (DAS)-enzyme-linked immunosorbent assay (ELISA) with antisera to PepMV. DAS-ELISA tests for PepMV of other solanaceous species were negative, except for one sample of pepino (Solanum muricatum). Mechanical inoculation of susceptible Lycopersicon esculentum cv. NE-1 plants with crude sap extracts of 20 of these samples confirmed that 15 of them (from the Departments of Apurimac, Arequipa and Moquegua) were infected with PepMV; these inoculated plants were also DAS-ELISA positive and, in most cases, developed symptoms. Thirteen of the infective extracts were obtained from plants of wild Lycopersicon species (three L. chilense, three L. chmielewskii, two L. parviflorum and five L. peruvianum) and one each from the cultivated species L. esculentum and S. muricatum. The wild Lycopersicon species are newly reported natural hosts of PepMV. Tests for the other six viruses were negative, except that two samples contained Tomato mosaic virus. Thus, PepMV occurs in Lycopersicon species in central and southern Peru, even in isolated wild populations. These results indicate that the virus is not new to the region and has an efficient mechanism of natural transmission.
    Journal of Phytopathology 03/2002; 150(2):49 - 53. · 1.00 Impact Factor
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    ABSTRACT: Tomato mosaic virus (ToMV) is a limiting factor for the success of pepino (Solarium muricatum) as a new crop. The effects of ToMV infection on total and marketable yield, fruit weight, length/width ratio and soluble solids content (SSC) have been studied in two commercial clones (`Sweet Long' and `Sweet Round'). ToMV infection depressed total yield in infected plants of `Sweet Long' (43.1%), while it had no effect on this trait in `Sweet Round' . Marketable yield was dramatically reduced by ToMV infection in both clones, 94% in `Sweet Long' and 100% in `Sweet Round'. Infected plants of clone `Sweet Long' had a lower weight than healthy plants. Although no differences in fruit weight were detected in `Sweet Round' between ToMV infected and healthy plants, many fruits from infected plants showed deformities. Changes in fruit length/width ratio and SSC as a result of ToMV infection were not relevant, but fruit quality was lower in infected fruits, most of which had corky-like flesh. Forty-two clones from cultivated (S. muricatum), wild (S. caripense and S. tabanoense) and interspecific hybrids were tested for ToMV resistance. All but seven clones (four from S. muricatum and three from interspecific hybrids S. muricatum × S. caripense) were susceptible. Non-susceptible clones showed variable degrees of resistance and developed hypersensitive local lesions. Among these clones the most promising as sources of variation for resistance to ToMV are those belonging to the cultivated species. Although no immunity was found, plants from these clones remained asymptomatic and absorbance values resulting from the DAS-ELISA tests in these plants were always lower than those of the susceptible control (cv. `Sweet Round'). These sources of resistance may be of great utility in developing commercial clones resistant to this severe disease affecting pepino.
    Euphytica 01/2001; 120(2):247-256. · 1.64 Impact Factor
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    ABSTRACT: Mechanical inoculation and transmission by thrips in a growth chamber were used in order to screen Lycopersicon peruvianumand Lycopersicon chilense germplasm for tomato spotted wilt virus (TSWV) resistance. Two highly aggressive Spanish TSWV isolates (HA-931100 and T-941117), having different restrictotypes were used. L. peruvianum accessions PI-126935, PI-126944, CIAPAN 16, PE-18 and CIAPAN 17 showed high resistance to both isolates in mechanical and thrips transmission. Their resistance appears useful in breeding programmes.
    Plant Breeding 09/1999; 118(5):425 - 429. · 1.18 Impact Factor
  • J. PROHENS, S. SOLER, F. NUEZ
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    ABSTRACT: We evaluated the effects of treatments with thermotherapy (80°C for 24 h) and dipping in sodium hypochlorite (NaOCl; 0.8% for 10 min) on the germination percentage, germination rate, vigour index and ToMV inactivation of seeds from three pepino (Solarium muricatum) accessions (96–5, B–2 and OV–8) and two wild relatives (S. tabanoense EC–26 and S. caripense EC–40). Thermotherapy decreased the germination percentage (especially in the wild species), germination rate in wild species but not in cultivated pepino, and vigour index in all cases. Sodium hypochlorite increased germination, except for nonthermotreated seeds of OV–8, improved the germination rate in wild species and the vigour index in all cases except for non thermotreated OV–8. Seed coats were altered by both treatments, especially by NaOCl. Thermotherapy was totally effective in the inactivation of seed-borne ToMV, while NaOCl was not. Thermotherapy combined with NaOCl allows effective seed disinfection of S. muricatum seeds without negatively affecting seed germination. For the wild species, although thermotherapy reduces germination, the higher number of seeds per fruit in these species reduces the magnitude of the problem. Even when no disinfection is necessary, treatments with NaOCl are always advisable as they improve germination.
    Annals of Applied Biology 05/1999; 134(3):299 - 305. · 2.15 Impact Factor
  • Source
    Salvador Soler, M. José Díez, Fernando Nuez
    Plant Disease 11/1998; 82(11):1199-1204. · 2.74 Impact Factor
  • Plant Disease 11/1998; 82(11):1281-1281. · 2.74 Impact Factor
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    ABSTRACT: Resistance to Tomato yellow leaf curl virus (TYLCV) and Tomato spotted wilt virus (TSWV), among other diseases, has been reported in Solanum peruvianum PI 126944. Introgression lines (ILs) from S. peruvianum PI 126944 into the genetic background of cultivated tomato (S. lycopersicum) are being developed. Several generations were derived from three interspecific hybrids previously obtained. A lot of crosses and embryo rescue were required until the third backcross, due to the high degree of incompatibility existing between tomato and PI 126944. Crosses between F1 plants were made to obtain a pseudo-F2 generation. The same procedure was followed up to the pseudo-F6 generation. Additional crosses between plants of different generations were made in order to increase progeny. Of 263 molecular markers tested, 105 were polymorphic between tomato and PI 126944. This set of polymorphic markers consisted of 90 simple sequence repeats (SSR) and 15 cleaved amplified polymorphic sequences (CAPS). The amount of the S. peruvianum genome was reduced in advancing generations and this was coupled in some cases with a reduction of incompatibility. However, the S. peruvianum genome was almost completely represented among the different plants of the most advanced generations. ILs will be basically developed from them. Some of the generations developed were resistant to TYLCV and TSWV.
    Euphytica · 1.64 Impact Factor

Publication Stats

132 Citations
32.73 Total Impact Points

Institutions

  • 2002–2013
    • Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana
      Valenza, Valencia, Spain
  • 1999–2009
    • Polytechnical University of Valencia
      • • Institute for the Preservation and Improvement of Valencian Agro-diversity (COMAV)
      • • Department of Biotechnology
      Valenza, Valencia, Spain
  • 2001
    • University of Valencia
      Valenza, Valencia, Spain