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ABSTRACT: A Gram-negative, non-spore-forming, aerobic, non-flagellated and rod- or oval-shaped bacterial strain, BB-MW24T, was isolated from coastal sediment in South Korea, and subjected to a polyphasic study. Strain BB-MW24T grew optimally at 30-37 °C, at pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain BB-MW24T clustered with the type strains of Ponticoccus litoralis and Roseivivax lentus. Strain BB-MW24T exhibited 16S rRNA gene sequence similarity values of 96.0 and 96.2 % to P. litoralis CL-GR66T and R. lentus S5-5T, respectively. Strain BB-MW24T exhibited the highest gyrB sequence similarity value (85.2 %) to Sagittula stellate DSM 11524T, and gyrB sequence similarity values of 83.3 and 83.2 % to P. litoralis DSM 18986T and R. lentus S5-5T, respectively. Strain BB-MW24T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipid profile strain BB-MW24T was similar with those of members of the genera Ponticoccus and Roseivivax, but one unidentified phospholipid as a major polar lipid was found only in strain BB-MW24T. The DNA G+C content was 68.9 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain BB-MW24T constitutes a new genus and species within the class Alphaproteobacteria, for which the name Litorisediminicola beolgyonensis gen. nov., sp. nov. is proposed. The type strain of Litorisediminicola beolgyonensis is BB-MW24T (= KCTC 32139T = CCUG 62953T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated DPS-8T, was isolated from coastal sediment of Geoje island in the South Sea, South Korea, and subjected to a polyphasic study. Strain DPS-8T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DPS-8T joined the clade comprising the type strains of Winogradskyella species by a high bootstrap resampling value of 93.5 %. Phylogenetic trees constructed using maximum-likelihood and maximum-parsimony algorithms revealed that strain DPS-8T belonged to the genus Winogradskyella. Strain DPS-8T exhibited 94.1-96.5 % 16S rRNA gene sequence similarity to the type strains of Winogradskyella species. Strain DPS-8T contained MK-6 as the predominant menaquinone and iso-C15:1 G, iso-C15:0, iso-C17:0 3-OH and C16:1 ω7c and/or iso-C15:0 2-OH as the major fatty acids. The major polar lipids of strain DPS-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain DPS-8T was 34.7 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain DPS-8T is separate from recognized Winogradskyella species. On the basis of the data presented, strain DPS-8T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella litorisediminis sp. nov. is proposed. The type strain is DPS-8T (= KCTC 32110T = CCUG 62215T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, designated WT-MW11T, was isolated from wood falls in the South Sea, Korea, and its taxonomic position was investigated by using a polyphasic study. Strain WT-MW11T grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2.0-3.0 % (w/v) NaCl. Bacteriochlorophyll a was not produced. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WT-MW11T clustered with Pseudorhodobacter ferrugineus IAM 12616T and Pseudorhodobacter aquimaris HDW-19T, with which it exhibited 16S rRNA gene sequence similarity values of 97.9 and 95.5 %, respectively. Strain WT-MW11T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The polar lipid profile of strain WT-MW11T was similar with those of P. ferrugineus JCM 20687T and P. aquimaris HDW-19T. The DNA G+C content was 61.6 mol% and its mean DNA-DNA relatedness value with P. ferrugineus JCM 20687T was 12.3 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WT-MW11T is distinguishable from the two recognized Pseudorhodobacter species. On the basis of the data presented, strain WT-MW11T is considered to represent a novel species of the genus Pseudorhodobacter, for which the name Pseudorhodobacter wandonensis sp. nov. is proposed. The type strain of Pseudorhodobacter wandonensis is WT-MW11T (= KCTC 23672T = CCUG 61506T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-negative, non-motile, non-spore-forming and rod-shaped bacterial strain, BB-Mw22T, was isolated from a tidal flat sediment of the South Sea in South Korea. It grew optimally at 30-37 °, pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-Mw22T belonged to the genus Kangiella and the cluster comprising Kangiella species and strain BB-Mw22T was clearly separated from other taxa. Strain BB-Mw22T exhibited 95.3-97.7 % 16S rRNA gene sequence similarity to the type strains of the five recognized Kangiella species. Strain BB-Mw22T contained Q-8 as the predominant ubiquionone and iso-C15:0 and iso-C11:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified aminolipid. The DNA G+C content of strain BB-Mw22T was 48.9 mol% and its mean DNA-DNA hybridization values with K. geojedonensis YCS-5T, K. japonica JCM 16211T and 'K. taiwanensis' JCM 17727T were 14-28 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain BB-Mw22T is distinguishable from the five recognized Kangiella species and 'K. taiwanensis'. On the basis of the data presented, strain BB-Mw22T is considered to represent a novel species of the genus Kangiella, for which the name Kangiella sediminilitoris sp. nov. is proposed. The type strain is BB-Mw22T (= KCTC 23892T = CCUG 62217T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, D1-y4T, was isolated from a tidal flat sediment of the South Sea in South Korea and subjected to a polyphasic study. Strain D1-y4T grew optimally at 25°C, pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain D1-y4T belonged to the genus Geatbulibacter, joining the type strain of Geatbulibacter marinus, with which it exhibited 97.8 % 16S rRNA gene sequence similarity. Sequence similarities to Gaetbulibacter saemankumensis SMK-12T and Gaetbulibacter aestuarii KYW382T were 96.5 and 96.2 %, respectively. Strain D1-y4T contained MK-6 as the predominant menaquionone and iso-C15:0, iso-C15:1 G and anteiso-C15:0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain D1-y4T was 34.6 mol% and its mean DNA-DNA relatedness value with G. marinus KCTC 23046T was 7 %. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain D1-y4T is distinguishable from the three recognized Gaetbulibacter species. On the basis of the data presented here, strain D1-y4T is considered to represent a novel species of the genus Geatbulibacter, for which the name Geatbulibacter lutimaris sp. nov. is proposed. The type strain is D1-y4T (= KCTC 23716T = CCUG 61504T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2012; · 2.11 Impact Factor
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ABSTRACT: A novel bacterial strain, designated T-Y1(T), capable of degrading a variety of polysaccharides was isolated from seawater of an oyster farm in the South Sea, Korea. It was found to be aerobic, Gram-negative, non-flagellated, non-gliding and rod-shaped. Strain T-Y1(T) grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain T-Y1(T) belonged to the genus Winogradskyella. Strain T-Y1(T) exhibited 16S rRNA gene sequence similarity values of 95.0-96.8 % to the type strains of recognized Winogradskyella species and less than 94.5 % to other validly named species. The chemotaxonomic data concurred with the phylogenetic inference. Strain T-Y1(T) contained MK-6 as the predominant menaquinone and anteiso-C(15:0), iso-C(15:0), iso-C(15:1) G and iso-C(16:0) 3-OH as the major fatty acids. The major polar lipids of strain T-Y1(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 36.2 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, enabled strain T-Y1(T) to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain T-Y1(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella multivorans sp. nov. is proposed. The type strain is T-Y1(T) (=KCTC 23891(T) = CCUG 62216(T)).
Antonie van Leeuwenhoek 04/2012; 102(2):231-8. · 2.09 Impact Factor
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ABSTRACT: A Gram-negative, non-motile, non-endospore-forming bacterial strain, designated DPSR-4(T), was isolated from a tidal flat sediment on the southern coast of Korea. Strain DPSR-4(T) grew optimally at 25-30°C, at pH 7.0-7.5 and in the presence of 2% (w/v) NaCl. A Neighbour-Joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DPSR-4(T) clustered with Salinimonas chungwhensis BH030046(T) by a high bootstrap resampling value of 99.7%. Strain DPSR-4(T) exhibited 96.2% 16S rRNA gene sequence similarity to that of S. chungwhensis BH030046(T) and 93.7-96.6% sequence similarity to the sequences of type strains of Alteromonas species. Strain DPSR-4(T) contained Q-8 as the predominant ubiquinone and iso-C(15:0) 2-OH and/or C(16:1) ω7c, C(16:0) and C(18:1) ω7c as the major fatty acids. The major polar lipids detected in strain DPSR-4(T) and S. chungwhensis KCTC 12239(T) were phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The DNA G+C content was 53.4 mol%. Differential phenotypic properties and phylogenetic distinctiveness of strain DPSR-4(T) demonstrated that this strain is distinguishable from the sole recognized species of the genus Salinimonas, S. chungwhensis. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPSR-4(T) is considered to represent a novel species of the genus Salinimonas, for which the name Salinimonas lutimaris sp. nov. is proposed. The type strain is DPSR-4(T) (KCTC 23464(T), CCUG 60743(T)).
Antonie van Leeuwenhoek 01/2012; 101(4):803-10. · 2.09 Impact Factor
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ABSTRACT: A Gram-negative, aerobic, non-flagellated, non-gliding rod, designated T-y2(T), was isolated from seawater of an oyster farm in the South Sea, Korea. Strain T-y2(T) grew optimally at 25 °C, at pH 7.0-7.5 and with 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T-y2(T) belonged to the genus Mesonia and exhibited 94.3-96.4% 16S rRNA gene sequence similarity with the type strains of species of the genus Mesonia. The DNA G+C content of strain T-y2(T) was 42.1 mol%. Strain T-y2(T) contained MK-6 as the predominant menaquinone and anteiso-C(15:0) and iso-C(15:0) as the major fatty acids. The only major phospholipid identified was phosphatidylethanolamine. The differential phenotypic properties and phylogenetic distinctiveness of strain T-y2(T) revealed that it is distinguishable from recognized members of the genus Mesonia. On the basis of the data presented here, strain T-y2(T) is considered to represent a novel species of the genus Mesonia, for which the name Mesonia ostreae sp. nov. is proposed. The type strain is T-y2(T) ( = KCTC 23500(T) = CCUG 60802(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2011; 62(Pt 8):1804-8. · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, non-flagellated, motile by gliding, aerobic rod, designated DPG-24(T), was isolated from seawater of Geoje Island in the South Sea, Korea. Strain DPG-24(T) grew optimally at 30-37 °C, at pH 7.0-7.5 and with 2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DPG-24(T) belonged to the genus Winogradskyella and clustered with Winogradskyella poriferorum UST030701-295(T) and Winogradskyella exilis 022-2-26(T). Strain DPG-24(T) exhibited 97.6 and 95.8% 16S rRNA gene sequence similarities with W. poriferorum UST030701-295(T) and W. exilis 022-2-26(T), respectively, and 92.4-95.7% with other members of the genus Winogradskyella. Strain DPG-24(T) contained MK-6 as the predominant menaquinone and iso-C(15:1) G, iso-C(15:0) and iso-C(17:0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The DNA G+C content was 36.7 mol%. DNA-DNA relatedness between the isolate and W. poriferorum JCM 12885(T) was 10.8%. The differential phenotypic properties and phylogenetic and genetic distinctiveness enabled strain DPG-24(T) to be differentiated from recognized members of the genus Winogradskyella. On the basis of the data presented, strain DPG-24(T) is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella aquimaris sp. nov. is proposed; the type strain is DPG-24(T) ( = KCTC 23502(T) = CCUG 60798(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2011; 62(Pt 8):1814-8. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-negative, aerobic, non-flagellated, non-spore-forming, motile (by gliding) bacterial strain, designated M-M6(T), was isolated from marine sand of Geoje island, Korea. Strain M-M6(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain M-M6(T) fell within the clade comprising Cellulophaga species, forming a coherent cluster with Cellulophaga lytica ATCC 23178(T) and Cellulophaga fucicola NN015860(T), with which it shared 16S rRNA gene sequence similarities of 98.1 and 98.2 %, respectively. Sequence similarities between strain M-M6(T) and the type strains of other recognized Cellulophaga species were in the range 92.4-93.8 %. Strain M-M6(T) contained MK-6 as the predominant menaquinone and iso-C(15:0), iso-C(15:1) G, iso-C(17:0) 3-OH, and C(16:1)ω7c and/or iso-C(15:0) 2-OH as the major fatty acids. The major polar lipids detected in strain M-M6(T) and the type strains of C. lytica and C. fucicola were two unidentified lipids, one unidentified aminolipid and one unidentified aminophospholipid. The DNA G+C content of strain M-M6(T) was 35.4 mol%. Levels of DNA-DNA relatedness between strain M-M6(T) and C. lytica JCM 8516(T) and C. fucicola JCM 21778(T) were 33 and 35 %, respectively. Differential phenotypic properties and phylogenetic and genetic distinctiveness distinguished strain M-M6(T) from all recognized Cellulophaga species. On the basis of the data presented, strain M-M6(T) is considered to represent a novel species of the genus Cellulophaga, for which the name Cellulophaga geojensis sp. nov. is proposed. The type strain is M-M6(T) ( = KCTC 23498(T) = CCUG 60801(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 62(Pt 6):1354-8. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-negative, non-motile, ovoid or rod-shaped bacterial strain, designated L-6(T), was isolated from seawater of Baekdo harbour of the East Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain L-6(T) grew optimally at 30 °C, at pH 7.5-8.0 and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain L-6(T) formed a cluster with the type strain of Celeribacter neptunius at a bootstrap resampling value of 100 %. Strain L-6(T) exhibited 16S rRNA gene sequence similarity values of 97.7 % to C. neptunius H 14(T) and of less than 96.2 % to the type strains of other species used in the phylogenetic analysis. The G+C content of the chromosomal DNA of strain L-6(T) was 60.9 mol%. The predominant ubiquinone found in strain L-6(T) and C. neptunius CIP 109922(T) was ubiquinone-10 (Q-10). The predominant fatty acid of strain L-6(T) and C. neptunius CIP 109922(T) was C(18:1)ω7c. The major polar lipids of strain L-6(T) were phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The mean level of DNA-DNA relatedness between strain L-6(T) and C. neptunius CIP 109922(T) was 17 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain L-6(T) is distinguishable from C. neptunius. On the basis of the data presented, strain L-6(T) is considered to represent a novel species of the genus Celeribacter, for which the name Celeribacter baekdonensis sp. nov. is proposed. The type strain is L-6(T) ( = KCTC 23497(T) = CCUG 60799(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 62(Pt 6):1359-64. · 2.11 Impact Factor
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ABSTRACT: The taxonomic position of a Gram-staining-negative, non-motile, rod-shaped bacterium, strain BB-My12(T), which was isolated from a sediment sample collected from a tidal flat in Korea, was investigated by using a polyphasic approach. Strain BB-My12(T) grew optimally at 37 °C, at pH 7.0-7.5 and in the presence of 2-3 % (w/v) NaCl. In phylogenetic analyses based on 16S rRNA gene sequences, strain BB-My12(T) fell within the cluster comprising species of the genus Muricauda and appeared most similar to the type strains of Muricauda aquimarina, Muricauda lutimaris and Muricauda ruestringensis (97.5-97.6 % sequence similarity). The DNA G+C content was 45.0 mol%. Strain BB-My12(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 1), iso-C(17 : 0) 3-OH and iso-C(15 : 0) as the major cellular fatty acids. The polar lipids of strain BB-My12(T) were phosphatidylethanolamine and four unidentified lipids. The DNA-DNA relatedness values between strain BB-My12(T) and the type strains of the three species of the genus Muricauda that appeared most closely related were in the range 5-7 %. The genetic distinctiveness and some phenotypic properties indicated that strain BB-My12(T) did not belong to any established species of the genus Muricauda. Strain BB-My12(T) is therefore considered to represent a novel species of the genus Muricauda, for which the name Muricauda beolgyonensis sp. nov. is proposed. The type strain is BB-My12(T) ( = KCTC 23501(T) = CCUG 60800(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2011; 62(Pt 5):1134-9. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-negative, aerobic, non-flagellated, non-gliding and short rod- or rod-shaped bacterial strain, designated BB-My20(T), was isolated from tidal flat sediment taken from the southern coast of Korea. Strain BB-My20(T) grew optimally at 37 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain BB-My20(T) fell within the clade comprising Salinimicrobium species, joining Salinimicrobium catena HY1(T), with which it had a 16S rRNA gene sequence similarity value of 97.4 %. It exhibited 95.4-96.9 % sequence similarity to the type strains of other members of the genus Salinimicrobium. Strain BB-My20(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), anteiso-C(15 : 0) and iso-C(17 : 0) 3-OH as the major fatty acids. The major polar lipids detected in strain BB-My20(T) and S. catena JCM 14015(T) were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain BB-My20(T) was 45.1 mol% and its mean DNA-DNA relatedness value with S. catena JCM 14015(T) was 4.5 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain BB-My20(T) can be distinguished from the four recognized species of the genus Salinimicrobium. On the basis of the data presented, strain BB-My20(T) is considered to represent a novel species of the genus Salinimicrobium, for which the name Salinimicrobium gaetbulicola sp. nov. is proposed; the type strain is BB-My20(T) ( = KCTC 23579(T) = CCUG 60898(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 62(Pt 5):1027-31. · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, non-spore-forming, rod-shaped bacterial strain, RL-2(T), was isolated from seawater of the East Sea in Korea and was subjected to a polyphasic taxonomic study. Strain RL-2(T) grew optimally at pH 7.5-8.0, at 20 °C and in the absence of NaCl. Phylogenetic trees based on 16S rRNA gene sequence analysis showed that strain RL-2(T) forms a cluster with Perlucidibaca piscinae IMCC1704(T) and various uncultured and unidentified gammaproteobacteria. Strain RL-2(T) exhibited 16S rRNA gene sequence similarity values of 96.1 % to Perlucidibaca piscinae IMCC1704(T) and 93.7-99.7 % to the uncultured bacterial clones belonging to the cluster and an unidentified gammaproteobacterium. The fatty acid profile of strain RL-2(T) was similar to that of Perlucidibaca piscinae IMCC1704(T), but the predominant ubiquinone type (Q-11) of strain RL-2(T) was different from that (Q-8) of Perlucidibaca piscinae IMCC1704(T). The DNA G+C content of strain RL-2(T) was 61.3 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain RL-2(T) is considered to represent a novel species of a new genus in the family Moraxellaceae, for which the name Paraperlucidibaca baekdonensis gen. nov., sp. nov. is proposed. The type strain of Paraperlucidibaca baekdonensis is RL-2(T) ( = KCTC 23145(T) = CCUG 59307(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2011; 61(Pt 6):1382-5. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-negative, non-motile, non-spore-forming, aerobic, rod-shaped bacterial strain, designated DPG-28T, was isolated from seawater on the southern coast of Korea. Strain DPG-28T grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DPG-28T formed a coherent cluster with members of the genera Marivita and Gaetbulicola, with which it exhibited sequence similarity values of 97.8-98.5 %. The DNA G+C content of strain DPG-28T was 65.1 mol%. The predominant ubiquinone of strain DPG-28T was ubiquinone-10 (Q-10), consistent with data for the genera Marivita and Gaetbulicola. The cellular fatty acid profiles of strain DPG-28T and the type strains of Marivita cryptomonadis, Marivita litorea and Gaetbulicola byunsanensis were essentially similar in that the common predominant fatty acid was C18:1ω7c. Major polar lipids found in strain DPG-28T and the type strains of M. cryptomonadis, M. litorea and G. byunsanensis were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. From these data, it is proposed that Gaetbulicola byunsanensis be reclassified as a member of the genus Marivita, for which the name Marivita byunsanensis comb. nov. is proposed, with the type strain SMK-114T (=CCUG 57612T=KCTC 22632T), and that strain DPG-28T be classified in the genus Marivita. Differential phenotypic properties and genetic distinctiveness of strain DPG-28T demonstrated that this strain is distinguishable from M. cryptomonadis, M. litorea and G. byunsanensis. On the basis of the data presented, strain DPG-28T is considered to represent a novel species of the genus Marivita, for which the name Marivita hallyeonensis sp. nov. is proposed. The type strain is DPG-28T (=KCTC 23421T=CCUG 60522T). An emended description of the genus Marivita is also provided.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2011; 62(Pt 4):839-43. · 2.11 Impact Factor
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ABSTRACT: Two Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterial strains, designated 3B-2(T) and 10AO(T), were isolated from a sand sample collected from the west coast of the Korean peninsula by using low-nutrient media, and their taxonomic positions were investigated in a polyphasic study. The strains did not grow on marine agar. They grew optimally at 30 °C and pH 6.5-7.5. Strains 3B-2(T) and 10AO(T) shared 97.5 % 16S rRNA gene sequence similarity and mean level of DNA-DNA relatedness of 12 %. In phylogenetic trees based on 16S rRNA gene sequences, strains 3B-2(T) and 10AO(T), together with several uncultured bacterial clones, formed independent lineages within the evolutionary radiation encompassed by the phylum Bacteroidetes. Strains 3B-2(T) and 10AO(T) contained MK-7 as the predominant menaquinone and iso-C(15 : 0) and C(16 : 1)ω5c as the major fatty acids. The DNA G+C contents of strains 3B-2(T) and 10AO(T) were 42.8 and 44.6 mol%, respectively. Strains 3B-2(T) and 10AO(T) exhibited very low levels of 16S rRNA gene sequence similarity (<85.0 %) to the type strains of recognized bacterial species. These data were sufficient to support the proposal that the novel strains should be differentiated from previously known genera of the phylum Bacteroidetes. On the basis of the data presented, we suggest that strains 3B-2(T) and 10AO(T) represent two distinct novel species of a new genus, for which the names Ohtaekwangia koreensis gen. nov., sp. nov. (the type species; type strain 3B-2(T) = KCTC 23018(T) = CCUG 58939(T)) and Ohtaekwangia kribbensis sp. nov. (type strain 10AO(T) = KCTC 23019(T) = CCUG 58938(T)) are proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2011; 61(Pt 5):1066-72. · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-negative, non-motile, non-spore-forming bacterial strain, DPG-3(T), was isolated from seawater from the South Sea in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain DPG-3(T) grew optimally at 30 °C and in the presence of 2% (w/v) NaCl. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain DPG-3(T) fell within a clade comprising Algoriphagus species and appeared most closely related to Algoriphagus halophilus JC 2051(T) (96.1%16S rRNA gene sequence similarity) and Algoriphagus lutimaris S1-3(T) (96.4%). The type strains of other Algoriphagus species showed 16S rRNA gene sequence similarities of 92.9-96.0% with strain DPG-3(T). The predominant menaquinone of strain DPG-3(T) was MK-7. The major fatty acids were iso-C(15:0) and iso-C(15:0) 2-OH and/or C(16:1)ω7c (summed feature 3). The major polar lipids detected in strain DPG-3(T) were phosphatidylcholine, phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content was 44.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPG-3(T) is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus namhaensis sp. nov. is proposed. The type strain is DPG-3(T) (=KCTC 23419(T)=CCUG 60523(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 3):575-9. · 2.11 Impact Factor
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ABSTRACT: A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, YCS-5(T), was isolated from seawater off the southern coast of Korea. Strain YCS-5(T) grew optimally at 30 °C and in the presence of 2% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YCS-5(T) fell within the clade comprising Kangiella species. Strain YCS-5(T) exhibited 16S rRNA gene sequence similarity values of 96.6, 95.7 and 97.9% to the type strains of Kangiella koreensis, Kangiella aquimarina and Kangiella japonica, respectively, and less than 89.8% to strains of other species used in the phylogenetic analysis. Strain YCS-5(T) contained Q-8 as the predominant ubiquinone and iso-C(17:0), iso-C(15:0), iso-C(11:0) 3-OH and iso-C(17:1)ω9c as the major fatty acids. The polar lipid profile of strain YCS-5(T) was similar to that of K. koreensis SW-125(T), with phosphatidylglycerol and an unidentified aminolipid as major polar lipids. The DNA G+C content was 47 mol%. The mean DNA-DNA relatedness value between strain YCS-5(T) and K. japonica JCM 16211(T) was 12%. Differential phenotypic properties and the phylogenetic and genetic distinctiveness of strain YCS-5(T) demonstrated that this strain is distinguishable from other Kangiella species. On the basis of the data presented, strain YCS-5(T) is considered to represent a novel species of the genus Kangiella, for which the name Kangiella geojedonensis sp. nov. is proposed; the type strain is YCS-5(T) (=KCTC 23420(T)=CCUG 60526(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 3):511-4. · 2.11 Impact Factor
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ABSTRACT: A gram-negative, non-spore-forming, non-motile rod, designated MA-My1(T), was isolated from a tidal flat sediment of the South Sea, Korea. Strain MA-My1(T) grew optimally at pH 7.0-7.5, at 30 °C and with 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MA-My1(T) clustered with Lutibacter litoralis CL-TF09(T) and Lutibacter maritimus S7-2(T), with which it exhibited 97.3 and 95.3 % 16S rRNA gene sequence similarity, respectively. Strain MA-My1(T) contained MK-6 as the predominant menaquinone and iso-C(15 : 0), iso-C(15 : 0) 3-OH and iso-C(16 : 0) 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 30.6 mol% and DNA-DNA relatedness between strain MA-My1(T) and L. litoralis JCM 13034(T) was 6.3±0.8 %. The differential phenotypic properties, together with phylogenetic and genotypic distinctiveness, distinguished strain MA-My1(T) from the members of the genus Lutibacter. On the basis of the data presented, strain MA-My1(T) is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter aestuarii sp. nov. is proposed. The type strain is MA-My1(T) ( = KCTC 23499(T) = CCUG 60022(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 2):420-4. · 2.11 Impact Factor
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ABSTRACT: A Gram-staining-variable, motile, endospore-forming and rod-shaped bacterial strain, IDS-20(T), was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic investigation. Strain IDS-20(T) grew optimally at 37 °C, at pH 7.5-8.0 and in the presence of 4-5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IDS-20(T) belongs to the genus Virgibacillus. Strain IDS-20(T) exhibited 93.4-96.6 % 16S rRNA gene sequence similarity to the type strains of species of the genus Virgibacillus. Strain IDS-20(T) had MK-7 as the predominant menaquinone and a cell-wall peptidoglycan based on meso-diaminopimelic acid. The major fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 39.5 mol%. The phylogenetic distinctiveness and differential phenotypic characteristics of strain IDS-20(T) demonstrated that this strain can be distinguished from recognized species of the genus Virgibacillus. On the basis of the data presented, strain IDS-20(T) represents a novel species of the genus Virgibacillus, for which the name Virgibacillus campisalis sp. nov. is proposed. The type strain is IDS-20(T) ( = KCTC 13727(T) = CCUG 59308(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2011; 62(Pt 2):347-51. · 2.11 Impact Factor
