[Show abstract][Hide abstract] ABSTRACT: Amiodarone is an anti-arrhythmic agent used for various types of tachyarrhythmia both ventricular and supra ventricular arrhythmias (fast forms of irregular heart beat). Present study is aimed to develop simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Amiodarone hydrochloride. Amiodarone was identified and quantitated on a C2 reversed phase column (4.6 × 250mm, 5.0µ), using a mobile phase composed of acetate buffer-Acetonitrile (15:85v/v) delivered at a flow rate of 1.0 mL/min, and with UV detection (lamda excitation= 240 nm). The method was proven to be linear over Amiodarone hydrochloride concentration range of 12.5 to 75µg/mL with a mean correlation coefficient of 0.9999. The present study is the development of HPLC method for the Pharmaceutical Formulation of Amiodarone Hydrochloride.
[Show abstract][Hide abstract] ABSTRACT: The aim of this study is to development and validation of a stability indicating RP-HPLC method for the determination of dissolution and assay of Sildenafil citrate tablets 50mg and 100mg using RP-HPLC with UV detector. Sildenafil citrate and related substances were baseline separated and quantitated on a C18 reverse phase column (4.6mm × 250mm, 5.0µm), using a mobile phase composed of a phosphate buffer-acetonitrile (40:60v/v,) delivered at a flow rate of 1.0mL/min, and with UV detection (lexcitation = 225nm). The method was proven to be linear over a Sildenafil citrate concentration range of 13 to 83µg/mL with a mean correlation coefficient of 0.9999.
[Show abstract][Hide abstract] ABSTRACT: In this present study forty nine different plants
used in traditional Indian medicine were examined
against Aspergillus niger using agar well diffusion
method. The methanolic extracts of 43 plants exhibited
varying degrees of inhibition activity against the fungi.
Among the forty nine plants studied 86% of the plants had
antifungal activity while the remaining 14% had no
antifungal activity. The extract from Grewia arborea
showed maximum activity. Emblica officinales,
Heldigordia populipolia, Hyptis sueolences, Moringa
heterophylla, Strychnos nuxvomica and Vitex negundo
did not exhibit antifungal activity at the condition studied.
Indian Journal of Science and Technology. 04/2009; 2:87-90.
[Show abstract][Hide abstract] ABSTRACT: Vitex agnus castus extracts in different solvent ratios were analyzed using High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in Vitexagnus castus are Agnuside, Casticin and Vitexyl acetone. To correlate the retention time of the molecular marker compounds with Vitex agnus castus extract were compared with standard molecular markers of Agnuside, Casticin and Vitexyl acetone using same conditions of that of extract and were analyzed statistically. Apart from the results ELS Detector is regarded as a valuable alternative to UV detection system for liquid chromatographic analysis of substances that doesnot contain a strong chromophore. As the markers do not contain strong chromophore, it is difficult to identify it UV detection system in High performance liquid chromatography. So an alternative is the Evaporative Light scattering Detector which is an effective tool for the identification of the compounds which do not contain chromophores. Present study concludes that ELS Detection system is more efficient than the UV/Vis detection system.
[Show abstract][Hide abstract] ABSTRACT: The antifungal activity of forty nine medicinal plants belonging to different families was tested in vitro on phytopathogenic fungusColletotrichum graminicola. In which methanolic extracts of forty two plants exhibited varying degrees of inhibition activity against C.graminicola. The results revealed that extract of Terminalia chebula was highly effective in inhibiting the mycelial growth of C. graminicola at 75µg/50µL. The following six plants Acalypha indica, Eichhornia crassipes, Gyanandropsis gyanandra, Suaeda maritime, Tephrosia pumila and Tinospora cordifolia did not exhibit antifungal activity.
[Show abstract][Hide abstract] ABSTRACT: Excoecaria agallocha L. leaves were extracted by various extracting procedures, using different solvents for testing the antimicrobial activities against important microorganisms using agar well diffusion method. Chloroform and methanolic extracts were found to be effective against these organisms, whereas hexane extracts were inactive. The purpose of this study was to find preliminary data for the development of alternative treatments to chemical microbicides for the control of plant diseases from natural plant extracts.
[Show abstract][Hide abstract] ABSTRACT: Asparagus racemosus is known as Shatavari in Sanskrit. Dried roots of A. racemosus were extracted with organic solvents in different ratios and were analyzed using high performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in A. racemosus are Shatavari I and Shatavari IV. As both the markers do not contain strong chromophore, it is difficult to identify it in the UV detection system of High performance liquid chromatography. Our research reports suggested that High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) is used as an alternative and effective tool for the identification of the compounds which do not contain chromophores. Present study concludes that ELS Detection system is more efficient than the UV/Vis detection system. INTRODUCTION Asparagus racemosus called shatavari in Sanskrit is a multi-branched, spinous under-shrub, bearing numerous succulent and tuberous roots. The plant contains triterpene saponins, which are phytoestrogen compounds. When domesticated, Asparagus in the vegetable aisle contains a measurable level of ecdysteroids and some of the 'wild' species of Asparagus have even more. The active portion for shatavari is the root, which is usually dried. It works not only as an aphrodisiac but also to increase the flow of milk in nursing women. It is prescribed for menopause and some type of menstrual cycle problems. The plant is demulcent, aphrodisiac and galactagogue (promotes secretion of milk). The result of a study suggests an oestrogenic effect of the herb on the female mammary gland and genital organs . A. racemosus is often used for infertility, threatened miscarriage, leucorrhoea and menopausal problems. It nourishes the ovum and increases fertility. Additionally, it is used for recuperation from a variety of diseases, as it is considered nutritive and anti-inflammatory. Markers of our interest are Shatavari I and Shatavari IV. As both the markers do not contain chromophore, it is difficult to identify it in the UV detection system of High performance liquid chromatography.