Takashi Harada

Tokyo Women's Medical University, Edo, Tōkyō, Japan

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Publications (18)34.83 Total impact

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    ABSTRACT: A 63-year-old healthy female patient presented with well defined itchy erythematous lesions on the area of her eyebrows. Her eyebrows had been tattooed two months before her visit to us. The lesions had previously been treated by application of steroid ointment and anti-histamine and steroid tablets by mouth without success. We suspected the lesions to be contact dermatitis caused by some metal element contained in the dye used for tattooing. Treatment was continued for two weeks, but the lesions spread to her cheeks and forehead. No fungal element was found from the lesions by direct microscopy at this stage. The patch-testing to 20 metal substances on her skin showed no allergic reaction. After one more week of treatment, we reexamined the scale taken from the lesions by direct microscopy, and fungal elements were found at that time. Microsporum (M.) gypseum was isolated from the scale taken from the lesions. The lesions cleared after treatment of 11 weeks' oral intake of itraconazole 100mg daily.It was found that the patient was accustomed to sleep with her dog, a Chihuahua. On examination by a veterinarian, no skin lesions were found on the dog. We speculate that the paws of the dog might have carried soil contaminated by M. gypseum, a geophilic fungus, to the area of her eyebrows which had minor trauma after being tattooed.
    Nippon Ishinkin Gakkai Zasshi 01/2012; 53(4):263-6.
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    ABSTRACT: We describe the simultaneous initial coexistence of mucous membrane pemphigoid (MMP) with blepharosynechia and anti-bullous pemphigoid (BP)180 antibodies and pemphigus vulgaris (PV) with lesions limited to the oral mucosa, with the presence of anti-desmoglein (Dsg)3 antibodies. A 75-year-old woman had severe oropharyngeal erosions and ulcers with blisters, and blepharoconjunctivitis and blepharosynechia of the left eye. Histopathological examination of the oral mucosa found acantholysis. Indirect immunofluorescence revealed IgG antikeratinocyte cell surface antibodies, and ELISA disclosed anti-Dsg3 antibodies. Immunoblotting found positive reactivity with recombinant proteins of both the BP180-NC16a domain and the BP180 C-terminal domain. To our knowledge, the simultaneous initial occurrence of MMP and PV has never been reported previously. We present a rare case of concurrent PV and anti-BP180 MMP, the diagnosis of which was confirmed by ELISA and immunoblotting assays.
    Clinical and Experimental Dermatology 09/2009; 34(8):e785-8. · 1.23 Impact Factor
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    ABSTRACT: We report two cases of candidal onychomycosis with severe nail deformities. Case 1: The patient was an 81-year-old man who complained of onycholysis and nail deformity of the right forefinger nail which had occurred over a period of a year. He had no obvious previous illness. Case 2: The patient was an 81-year-old woman who complained of nail deformity with periungual erythema which had occurred over a period of several months. She had been treated with oral corticosteroid for bronchial asthma and with Ca blocker for hypertension for a long period. The initial KOH-prepared direct microscopy in each case failed to detect any spores or pseudohyphae. Therefore, an incisional biopsy was performed in both cases. Histopathological findings demonstrated numerous fungal elements with similar appearance of dermatophytes in the middle to lower level of the horny cell layer by PAS and Grocott staining in each case. Candida albicans was isolated and identified by cultivation on ATG agar. In case 1, oral itraconazole (100 mg/day) was administered for 14 weeks, which was effective clinically and mycologically. In case 2, however, a coadministered drug (Ca blocker), oral terbinafine (125 mg/day) was not effective mycologically. Therefore, after having changed the antihypertensive agent, oral itraconazole (100 mg/day) was administered for 16 weeks, which was effective clinically and mycologically.
    Nippon Ishinkin Gakkai Zasshi 01/2009; 50(4):253-7.
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    ABSTRACT: We report a case of inflammatory tinea corporis by Epidermophyton ( E. ) floccosum. A 73-year-old man visited our hospital with an eruption on his left arm where he wore his wristwatch. He had treated himself with a topical steroid. On KOH preparation, many hyphal elements were observed in the scale. Histological findings revealed granulomatous inflammation around hair follicles and positive fungal elements by PAS and Grocott staining in these follicles. Topical luliconazole and oral terbinafin were effective clinically and mycologically. Epidemiological survey in Japan has shown the number of cases of tinea caused by E. floccosum to be on the decrease recently. The anthropophilic dermatophyte, E. floccosum is known usually not to invade the hair follicle, and no case of inflammatory tinea corporis by this species has been reported in Japan. We consider this case of inflammatory tinea corporis due to E. floccosum to be very rare.
    Nippon Ishinkin Gakkai Zasshi 02/2008; 49(3):211-5.
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    ABSTRACT: We herein report a case of tinea capitis initially showing a couple of nodular lesions. The patient was a 66-year-old woman who had seen a nearby dermatologist for itching on her head and had been treated with a topical steroid followed by tacrolimus application for one month. Because pseudolymphoma-like erythematous nodules developed at two sites, she visited us. Two weeks after stopping all medication, some slight scaling was found around these nodules. On KOH direct microscopic examination, many filamentous elements around hair shafts were observed. Biopsy of the nodules confirmed the destruction of hair follicles surrounded by granulomatous inflammation histologically. Grocott staining of the same specimen revealed a few short fungal hyphae as well as spores. She was also diagnosed as tinea pedis by direct microscopic examination of her feet. Trichophyton rubrum was isolated from scales of both her head and feet on Sabouraud's dextrose agar at 25 degrees C. Kerion celsi (KC) is usually clinically preceded by a gray patch or black dots. Such a typical course of KC, however, was not observed in our patient. Tacrolimus was thought to have possibly played an important role in modifying tinea capitis.
    Nippon Ishinkin Gakkai Zasshi 02/2004; 45(3):177-80.
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    ABSTRACT: Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation, and fragility of the skin. Linkage and homozygosity analysis in an isolated Panamanian cohort and in additional inbred families mapped the gene to 20p12.3. Loss-of-function mutations were identified in the FLJ20116 gene (renamed "KIND1" [encoding kindlin-1]). Kindlin-1 is a human homolog of the Caenorhabditis elegans protein UNC-112, a membrane-associated structural/signaling protein that has been implicated in linking the actin cytoskeleton to the extracellular matrix (ECM). Thus, Kindler syndrome is, to our knowledge, the first skin fragility disorder caused by a defect in actin-ECM linkage, rather than keratin-ECM linkage.
    The American Journal of Human Genetics 08/2003; 73(1):174-87. · 10.99 Impact Factor
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    ABSTRACT: We report two cases of tinea capitis by Microsporum (M) ferrugineum. A two year old boy (case 1) visited our hospital 3 months after his return from Myanmar. About a year later, we observed the same symptom on the scalp of his mother (case 2). In both cases itraconazole was effective clinically and mycologically. In Japan, although M. ferrugineum was the most common organism of tinea capitis before the 1960s, no cases of tinea are found caused by this species nowdays. We believe these cases to have been infected in Myanmar, and suggest the possibility that tinea caused by M. ferrugineum may become a re-emergent infection or an imported fungal disease in Japan.
    Nippon Ishinkin Gakkai Zasshi 02/2003; 44(3):203-7.
  • Nishi Nihon Hifuka 01/2003; 65(2):134-137.
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    ABSTRACT: The fungal elements observed by direct microscopic examination sometimes fail to grow on subsequent culture. To elucidate the cause of this discrepancy, the viability of dermatophytes and Candida in skin scales was evaluated by neutral red staining. Autoradiographic study using 3H-thymidine confirmed that grain-positive cells (viable cells) were stained with neutral red, whereas negative cells (non-viable cells) were not stained. Taking this as a baseline, the correlation between neutral red-positive fungal elements in scales taken from 211 patients with tinea and 27 patients with cutaneous candidosis and cultures on Sabouraud glucose agar was studied. Strong positive correlations were found in both mycoses. These findings suggest that neutral red staining provides a useful method for evaluating the viability of dermatophytes and Candida in human skin scales.
    Journal of medical and veterinary mycology: bi-monthly publication of the International Society for Human and Animal Mycology 02/1994; 32(1):31-5.
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    ABSTRACT: We describe an 82-year-old man with CD8+ lymphoepithelioid lymphoma localized to the skin. The skin lesion had been present for 18 years. Histologically there was a dense infiltrate that consisted of atypical lymphoid cells and large epithelioid cells, thereby resembling the so-called Lennert's lymphoma. Local radiotherapy proved effective in reducing the size of the tumor. To the best of our knowledge, this is the first report of primary lymphoepithelioid lymphoma of the skin.
    Journal of the American Academy of Dermatology 12/1993; 29(5 Pt 2):871-5. · 5.00 Impact Factor
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    ABSTRACT: We report a patient with vesicular pemphigoid who showed clinically numerous small vesicles and figurate erythema over the trunk and extremities. Although large blisters were absent, immunofluorescent study revealed IgG and C3 deposition at the basement membrane zone of the lesional skin. The patient responded well to the combined use of corticosteroid and dapsone. Immunoelectron microscopy showed IgG and C3 deposition at the undersurface membrane of the basal cells and the lamina lucida. Electron microscopy of the nonvesiculated early erythematous skin showed small vacuoles and lacunae along the uppermost portion of the dermis. Although the clinical features of this patient and the good therapeutic response to dapsone were atypical, the results of immunopathology and immunoelectron microscopy were consistent with typical pemphigoid.
    Dermatology 01/1989; 178(4):213-216. · 1.69 Impact Factor
  • Wataru Naka, Takashi Harada, Takeji Nishikawa
    Nishi Nihon Hifuka 01/1987; 49(4):632-636.
  • Japanese Journal of Medical Mycology. 01/1986; 27(4):265-267.
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    ABSTRACT: Prurigo pigmentosa is an inflammatory dermatosis characterized by pruritic, reddish, papular lesions and gross reticular pigmentation that occurs mainly on the trunk. Nearly 100 cases have been reported in Japan to date. We describe a patient with this condition who responded well to dapsone. An electron microscopic study of the reddish papular lesion showed marked intercellular edema and evidence of cellular injury of the basal cells. Direct immunofluorescence was negative. We stress that this condition is a distinct clinical entity, histologically characterized by a lichenoid tissue reaction as proposed by Pinkus, although it is little known outside Japan.
    Journal of the American Academy of Dermatology 02/1985; 12(1 Pt 2):165-9. · 5.00 Impact Factor
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    ABSTRACT: Eight out of 12 serum samples from patients with bullous pemphigoid having basement membrane zone antibodies gave positive binding not only to the basement membrane zone but also to the basal cell membrane and/or cytoplasm as observed by complement immunofluorescence. Reaction of fluorescein labeled pemphigus vulgaris -globulins, binding mainly to the lower inter-cellular spaces of the epidermis, was greatly reduced by the prior incubation of high-titered bullous pemphigoid sera having the reactivity to the basal cells, while that of fluorescein labeled pemphigus foliaceus -globulins binding mainly to the upper and middle intercellular spaces, was not influenced by the prior application of these bullous pemphigoid sera. These results indicate that bullous pemphigoid antibodies are heterogenous and can be classified into 2 types and that some cross reaction is present between pemphigus antibodies and bullous pemphigoid antibodies having the reactivity to the basal cells.
    Journal of Investigative Dermatology 08/1981; 77(2):251-251. · 6.37 Impact Factor
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    ABSTRACT: The capability of complement fixation of pemphigus antibodies was tested using combined in vitro complement immunofluorescent (IF) staining methods. Three sera out of 25 serum samples from 22 pemphigus patients revealed positive reactions, while all other sera gave negative results. Specificity control tests confirmed the positive reactions to be specific for complement staining. Complement fixing pemphigus antibodies were titrated lower than corresponding IgG antibodies and were demonstrable only in the extensive stage of the disease. Thus, the present work supplied evidence that pemphigus antibodies fix complement in vitro. However, the discrepancy still remains between the in vivo deposition of complement in most cases of pemphigus and in vitro capability of complement fixation in only few cases. More investigations should be needed to explain the exact role of complement in pemphigus acantholysis.Mit Hilfe von dem kombinierten in vitro-Immunofluores-cenzkomplementfixationstest wurde in vitro-Bindung von Komplement durch Pemphigusantikrper untersucht. Drei Seren aus den 25 Seren von 22 Patienten mit Pemphigus zeigten positive Bindung von Komplement (K3), whrend die brigen Seren negative Befunde zeigten. Die Spezifittskontrolltests besttigten, da die positive Reaktionen spezifisch fr Komplementfixation waren. Diese Komplementbindungsantikrper zeigten einen niedrigeren Titer als die korrespondierenden IgG-Antikrper und konnten nur im ausgedehnten Krankheitszustand gefunden werden. Diese Ergebnisse belegten, da Pemphigusantikrper das Komplement in vitro fixieren. Jedoch gibt es Diskrepanzen zwischen in vivo-Komplementsvorkommen und in vitro-Komplementsbindung. Die genaue Rolle des Komplements bei der Pemphigusacantholyse mu noch weiter untersucht werden.
    Archives for Dermatological Research 04/1979; 264(2):261-2. · 2.27 Impact Factor
  • Archives for Dermatological Research 01/1979; 264(2):261-262. · 2.27 Impact Factor
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    ABSTRACT: We applied the freeze-substituion method to the detailed observation of the ascosporogenesis in Arthroderma simii. The ascospore formation of A. simii underwent the same processes as did that of other dermatophytes, namely an enveloping membrane system (EMS) composed of two invaginated unit membra-nes that delimit the daughter nuclei in the cytoplasm of the ascus. The inner lea-flet of the EMS was found to have changed into an ascospore plasma membrane, whereas the outer leaflet and intercisternal space became the ascos-pore cell wall. In the freeze-substituted asci, we could not observe the lomasomal structure thought to be the origin of the EMS in dermatophytes.