S S Kang

Gyeongsang National University, Shinshū, Gyeongsangnam-do, South Korea

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Publications (55)119.26 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The study aimed to evaluate the efficacy of recombinant adenovirus expressing αA-crystallin (Ad-αAc-Gfp) in reducing pericyte loss within retinal vasculature in early diabetes. Diabetes was induced by streptozotocin injection into C57BL/6 mice. Ad-αAc-Gfp was delivered by intravitreous injection to the right eyes of mice 2 weeks before induction of diabetes. Vascular leakage was determined by fluorescent angiography, Evans Blue leakage assay and leucocyte adhesion test. Production of αA-crystallin was analysed by immunoblotting and double immunostaining and pericyte loss was analysed by pericyte count. Vessel leakage and pericyte loss were observed in the streptozotocin-induced diabetic retina. Decreased abundance of αA-crystallin in retinas 2 and 6 months after the induction of diabetes was confirmed by two-dimensional electrophoretic analysis, immunoblotting and RT-PCR. Double immunofluorescence staining for αA-crystallin and NG2 chondroitin sulphate proteoglycan revealed that αA-crystallin was predominantly produced in the retinal pericyte and that the number of αA-crystallin-producing pericytes decreased in the diabetic retina. Retinal infection with Ad-αAc-Gfp led to decreased pericyte loss and vascular leakage compared with control. Intravitreal delivery of Ad-αAc-Gfp protects against vascular leakage in the streptozotocin-induced model of diabetes. This effect is associated with the inhibition of diabetic retinal pericyte loss in early diabetes, suggesting that αA-crystallin has a role in preventing the pathogenesis of early diabetic retinopathy.
    Diabetologia 07/2012; 55(10):2835-44. DOI:10.1007/s00125-012-2625-y · 6.88 Impact Factor
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    ABSTRACT: In recent, a digital x-ray detector attracted worldwide attention and there are many studies to commercialize. There are two methods in digital x-ray detector. This method is an Indirect method and Direct method. This study is to see the differences between the digital x-ray detector based on a-Se used in the existing indirect conversion method and an x-ray conversion material that has better SNR(Signal-to-noise ratio) and property than the a-Se. To solve the problem that is difficult to make a large area film using Screen-Print method, we used a Screen-Print method. In this study, we used a polyclystal as x-ray conversion material and a sample thickness is and an area is . ITO(Indium-Tin-Oxide) electrode was used as top electrode using a Magnetron Sputtering System and each area is , and and then we evaluated darkcurrent, sensitivity and SNR of the film are measured, then we evaluated the electrical properties. And we used a current integration mode when I-V test. This experiment shows that the sensitivity increases in accordance with the area of the electrode but the SNR is decreased because of the high darkcurrent. Through fabricating of various thicknesses and optimal electrodes, we will optimize SNR in the future work.
    01/2010; 4(1).
  • S S Kang, J S Kim, E B Lee
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    ABSTRACT: In addition to three known saponins, araloside A, stipuleanosides R 1 and R 2, two new saponins, named durupcosides A and B, have been isolated from the root bark of Aralia elata as their methylesters. Their structures have been established as 3-O-ß-D-glucopyranosyl (1?2)-[a-L-arabinofuranosyl (1?4)]-ß-D-glucuronopyranosyl oleanolic acid and 3-O-a-L-arabinopyranosyl (1?2)-[ß-D-glucopyranosyl (1?3)]-ß-D-glucuronopyranosyl oleanolic acid, respectively, on the basis of spectroscopic data and chemical reactions.
    Pharmaceutical Biology 09/2008; 34(2):119-123. DOI:10.1076/phbi.34.2.119.13202 · 1.34 Impact Factor
  • Planta Medica 07/2008; 74(09). DOI:10.1055/s-0028-1084028 · 2.34 Impact Factor
  • Planta Medica 07/2008; 74(09). DOI:10.1055/s-0028-1084051 · 2.34 Impact Factor
  • Planta Medica 07/2008; 74(09). DOI:10.1055/s-0028-1084099 · 2.34 Impact Factor
  • Planta Medica 07/2008; 74(09). DOI:10.1055/s-0028-1084100 · 2.34 Impact Factor
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    ABSTRACT: Paeony root has long been used for its anti-inflammatory effects. In this study, the effects of albiflorin, paeoniflorin, and paeonol, compounds from paeony root, on gene expression profiles were examined in macrophages challenged with the inflammation inducer lipopolysaccharide (LPS). The RAW264.7 macrophages were treated with LPS in the presence or absence of albiflorin, paeoniflorin, or paeonol. Global mRNA expression levels were detected by using an oligonucleotide microarray platform covering the mouse whole genome. Treatment with LPS caused expression level changes in 1,270 genes by 2 folds or more. Paeonol attenuated the induction level of 355 LPS-responsive genes. Classification of the genes targeted by paeonol according to the Panther group analysis revealed 20 biological processes, 24 molecular functions, and 22 signaling pathways. The Panther signaling pathways highly affected by paeonol included the 'inflammation mediated by chemokine and cytokine signaling', 'interleukin signaling', and 'Toll receptor signaling'. Our results demonstrate that paeonol has extensive inhibitory effects on the regulation of inflammation associated gene expression by LPS in macrophages. In addition, the predominant effect of paeonol among the tested compounds suggests that paeonol may be a major ingredient for the anti-inflammatory effect of paeony root.
    Inflammation Research 04/2008; 57(4):189-98. DOI:10.1007/s00011-007-7190-3 · 2.14 Impact Factor
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    ABSTRACT: We investigated the relationship between phosphorylation of alphaB-crystallin (αBC) and retinal apoptosis in type 2 diabetes. The retinas of male Otsuka Long–Evans Tokushima fatty (OLETF) rats at 24 and 35 weeks were used as an animal model for type 2 diabetes and sex- and age-matched Long–Evans Tokushima Otsuka (LETO) rats were used as controls. In the retinas of 35-week OLETF rats, the interaction between αBC and protein kinase C delta (PKC δ) among the PKC isozymes, αBC phosphorylation at Ser45 (S45p-αBC), TUNEL-positive apoptotic ganglion cells, several apoptotic signs, and co-localization of S45p-αBC and TUNEL significantly increased as compared with other groups while the αBC–Bax interaction greatly decreased. These changes were abolished by rottlerin treatment, a highly specific PKC δ inhibitor. These results suggest that PKC δ is involved in regulation of anti-apoptotic function of αBC in the retina of type 2 diabetes.
    Neurobiology of Disease 01/2008; 28(3-28):293-303. DOI:10.1016/j.nbd.2007.07.017 · 5.20 Impact Factor
  • EJC Supplements 09/2007; 5(4):104-104. DOI:10.1016/S1359-6349(07)70489-5 · 9.39 Impact Factor
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    ABSTRACT: Streptozotocin (STZ) has been commonly used to induce in vivo and in vitro hyperglycemic diabetes and its toxicity leads to inflammation and vascular injury. Triamcinolone acetonide (TA), as an anti-angiogenic/anti-inflammatory drug, is clinically used to improve the visual acuity in neovascular and edematous ocular diseases. The aim of this study was to investigate the effect of TA on early inflammation and vascular leakage in the retina of STZ-induced hyperglycemic rats. Hyperglycemia was induced in 8-week-old male Sprague–Dawley (SD) rats by a single intraperitoneal injection of STZ (65 mg/kg); only rats with blood glucose levels > 13.9 mmol/l 1 day after STZ injection were included in STZ-hyperglycemic group. Sex- and age-matched SD rats injected with buffer were used as the control group. One day before STZ and buffer injection, 2 μl TA (4 mg/ml in saline) and 2 μl saline were intravitreal-injected into the right and the left eyes of rats, respectively. Retinal vascular leakage was measured using the Evans-blue method. Changes in pro-inflammatory target genes, such as tumor necrotic factor (TNF)-α, intracellular adhesion molecule (ICAM)-1, and vascular endothelial growth factor (VEGF) were assessed by immunoblottings, immunostaining, and ELISA analyses. Vascular hyperleakage and up-regulation of most pro-inflammatory genes peaked within a few days after STZ injection and had recovered. However, these changes were blocked by TA pretreatment. Our data suggest that TA controls STZ-induced early vascular leakage and temporary pro-inflammatory signals in the rat retina.
    Life Sciences 09/2007; DOI:10.1016/j.lfs.2007.08.024 · 2.30 Impact Factor
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    ABSTRACT: We examined the effect of triamcinolone acetonide (TA), a corticosteroid, on the relationship between vascular pathophysiology and vascular endothelial growth factor (VEGF) activation in the retina of a rat model of oxygen-induced retinopathy (OIR). OIR was induced by exposure of hyperoxia (80% oxygen) to Sprague-Dawley (SD) rats from P2 to P14 and then returned to normoxic conditions. TA was intravitreal-injected once into the right eye of OIR rats at P15. Effects of TA on vascular pathophysiology or changes of various genes in response to hypoxia and/or proinflammation under hypoxic retina were assessed by the Evans-blue method, fluorescein isothiocyanate-dextran (FITC-D) infusion, immunoblotting, and ELIZA. TA not only reduced retinal neovascularization and vascular leakage in the OIR-rat retina, but also blocked the induction of hypoxia-response proinflammatory genes before it negatively controlled VEGF activation. These findings suggest a potential that TA suppresses retinal neovascular pathophysiology via proinflammation-mediated activation of VEGF during hypoxia.
    Neurobiology of Disease 07/2007; 26(3):569-76. DOI:10.1016/j.nbd.2007.02.002 · 5.20 Impact Factor
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    ABSTRACT: We present our experience with the neuronavigation system as used for surgery of distal anterior cerebral artery (DACA) aneurysm. Between 2001 and 2004, 12 patients with a DACA aneurysm were consecutively treated with direct clipping assisted by the neuronavigation system. We used the BrainLAB Vector Vision neuronavigation system (BrainLAB, Heimstetten, Germany). Seven out of 12 patients presented with subarachnoid hemorrhage. Aneurysms were located at distal A2 in 10 patients and distal A3 in two patients. The size of the aneurysms ranged from 3-10 mm. There were no procedure-related complications or technical problems during application of the neuronavigation system. The registration accuracy ranged from 0.5-1.5 mm (mean: 0.88 mm). The neuronavigation system provided real-time presentation of the DACA and the aneurysm, and allowed for identification of the DACA aneurysm in all patients. No surgical complications developed, and all 12 patients had a good recovery after direct clipping. Although current neuronavigation systems are not available for all intracranial aneurysms, we believe that the DACA aneurysm is a good candidate for its use. The additional benefits of a small craniotomy and precise intraoperative orientation during surgery result in a minimally invasive aneurysm procedure.
    min - Minimally Invasive Neurosurgery 07/2007; 50(3):140-4. DOI:10.1055/s-2007-985151 · 1.14 Impact Factor
  • Materials Science Forum 01/2007; DOI:10.4028/www.scientific.net/MSF.544-545.331
  • Korean Journal of Materials Research 09/2006; 16(9):584-591. DOI:10.3740/MRSK.2006.16.9.584
  • S H Shim, J S Kim, S S Kang, K H Son, K Bae
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    ABSTRACT: A new diterpenoid alkaloid, jaluenine (1), has been isolated from the roots of Aconitum jaluense. The structure of jaluenine was determined by spectroscopic methods including two dimensional NMR (1H-1H COSY, HMQC, HMBC, NOESY).
    Journal of Asian Natural Products Research 07/2006; 8(5):451-5. DOI:10.1080/10286020500173275 · 0.97 Impact Factor
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    ABSTRACT: To investigate how and to what extent platycodin saponin (PS) from Platycodi Radix exerts a favorable influence on obesity and hyperlipidemia. Sprague-Dawley rats were fed with a high fat (HF) diet for 4 weeks and then the animals were treated with 35 or 70 mg / kg of PS for another 4 weeks. Changes in body weight and daily calorie intake were measured regularly during the experimental period and the degree of linear correlation for the above two variables was further analyzed. The in vitro lipase inhibition of each PS compound and the in vivo fecal lipid excretion were examined in hope of revealing their relationship. The concentrations of hepatic triglyceride and cholesterol in serum. The body weight reduction (13+/-4% vs HF control, P<0.05) by PS administration was highly correlated to the food intake restriction (Pearson's linear coefficient r=0.752, P<0.005). The in vitro inhibition of lipase by each isolated compound and mixture of PS were virtually identical. Consequently, the fecal TG excretion was increased by 2.1-3.2 folds depending on the dose of PS. The serum TG and LDL-cholesterol concentrations were decreased without noticeable changes in HDL-cholesterol levels. Concomitantly, the contents of the hepatic TG, cholesterol, and the liver surface fat pads were decreased in ubiquity, but no noticeable biochemical abnormalities or histological tissue damages were observed. The administration of PS produced profound effects on the control of obesity and lipid metabolism, which resulted in LDL-cholesterol reduction. PS also caused a remarkable reduction in calorie intake, which was highly correlated to the body weight loss. These results suggest that PS has a greater role in anti-obesity, hypolipidemia, and liver protection than previously thought. Hence, PS could be a potential therapeutic alternative in the treatment of obesity and hyperlipidemia.
    International Journal of Obesity 09/2005; 29(8):983-90. DOI:10.1038/sj.ijo.0802948 · 5.39 Impact Factor
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    ABSTRACT: The present study aimed to investigate the expression levels of and the relationship between 14-3-3 zeta and protein kinase C (PKC) in the retina of early diabetes. Changes in the expression levels of, and interaction between, 14-3-3 zeta and PKC were investigated by Northern and Western blot analyses, immunoprecipitation and double immunostaining in the retina of diabetic rats after 6 weeks of diabetes. PKC activity was examined using a PKC assay. In the diabetic retina, the molecular levels of 14-3-3 zeta were reduced, while those of PKC beta and zeta were increased. Direct interaction between 14-3-3 zeta and PKC was markedly decreased in the retina after 6 weeks of diabetes, while PKC activity was increased. These findings show that a reduction in 14-3-3 zeta can induce PKC activation, suggesting that this is a main cause of visual dysfunction in the retina during diabetes.
    Diabetologia 08/2005; 48(7):1411-5. DOI:10.1007/s00125-005-1774-7 · 6.88 Impact Factor
  • IY Kim, S Jung, K S Moon, TY Jung, S S Kang
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    ABSTRACT: We have applied the neuronavigation system to endoscopic biopsy and third ventriculostomy in the management of patients with a pineal tumor with hydrocephalus. With the guidance of neuronavigation, the two optimal sites of burr hole and trajectories were planned preoperatively, and the advancing endoscopic device was monitored in real time during the procedure. In our five patients, the diameters of the tumors were 2-3 cm, and the mean systemic accuracy of registration with neuronavigation was 1.2 mm. The biopsy and third ventriculostomy were performed successfully via the respective optimal burr hole and the trajectory determined using preoperative neuronavigation. There were no procedure-related complications, and none of the patients needed another procedure for CSF diversion during the follow-up periods. We present our technique which includes the application of the neuronavigation system to the biopsy and third ventriculostomy in pineal tumor with associated hydrocephalus. This technique can be performed using a simple rigid endoscope via the determined optimal entries and trajectories. The optimal preoperative planning and the intraoperative guidance by neuronavigation are thought to be able to give more chances to minimize the brain injury related to movements or deviation of endoscopic device.
    min - Minimally Invasive Neurosurgery 01/2005; 47(6):365-8. DOI:10.1055/s-2004-830150 · 1.14 Impact Factor

Publication Stats

992 Citations
119.26 Total Impact Points

Institutions

  • 1999–2012
    • Gyeongsang National University
      • College of Veterinary Medicine
      Shinshū, Gyeongsangnam-do, South Korea
    • Sangji University
      Genshū, Gangwon, South Korea
  • 1994–2008
    • Seoul National University
      • • Department of Dentistry
      • • College of Pharmacy
      • • College of Natural Sciences
      • • Department of Pharmacology
      Sŏul, Seoul, South Korea
  • 2007
    • Kwandong University
      Gangneung, Gangwon-do, South Korea
    • Chonnam National University
      • Department of Neurosurgery
      Gwangju, Gwangju, South Korea
  • 2002
    • Korea Institute of Nuclear Safety
      Sŏul, Seoul, South Korea
  • 1998–2001
    • Chonnam National University Hospital
      Sŏul, Seoul, South Korea
  • 1994–1999
    • Andong National University
      • Department of Food and Nutrition
      Antō, Gyeongsangbuk-do, South Korea
  • 1995
    • University of Seoul
      Sŏul, Seoul, South Korea