ABSTRACT: The present study aims to investigate the anti-proliferative, apoptotic properties of prodigiosin,
using a human oral squamous cell carcinoma HSC-2 cell line as a model system. HSC- 2 cells
were cultured in the presence of prodigiosin at various concentrations (1–30μg/ml) for 48 h and
the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide (MTT) assay. The results showed that prodigiosin inhibited the cells viability
in time and concentration dependent characteristics at different concentrations. We found that
anti-proliferative effect of prodigiosin was associated with apoptosis on HSC-2 cells by
determinations of DNA fragmentation, Hoechst 33258 staining, caspases activity, and TNF-α was
significantly changed when compare DMSO control group. In addition, activity of lactate
dehydrogenase (LDH) release increased when the cells incubated with prodigiosin at various
concentrations and times. These results suggested that prodigiosin treatment inhibited
proliferation and induction of apoptosis in human oral squamous cell carcinoma HSC-2.
INTERNATIONAL JOURNAL OF CURRENT RESEARCH. 01/2011; 3(4-ISSN: 0975-833X):151.