L V Bonamin

Publications (11)11.34 Total impact

• Article: Ultra high dilution of triiodothyronine modifies cellular apoptosis in Rana catesbeiana tadpole tail in vitro.

  J R P Guedes, S Carrasco, C M Ferreira, L V Bonamin, W Souza, C Goldenstein-Schainberg, E R Parra, V L Capelozzi
  [show abstract] [hide abstract]
  ABSTRACT: Ultra High Dilutions (UHD) are diluted beyond the Avogadro limit with dynamization (dilution with succussion). The process of anuran amphibian metamorphosis is controlled by thyroid hormones, including the resorption of the tadpole tail. A randomized and blinded study was performed to investigate the influence of triiodothyronine (T3) 5·10(-24)M (10cH) on apoptosis induced by T3 100 nM in Rana catesbeiana tadpoles' tail tips, in vitro. Explants were randomized to three groups: control: no T3 in pharmacological or UHD dose; test: T3 100 nM and challenged with T3 10cH (UHD); positive control: T3 100 nM, treated with unsuccussed ethanol. The apoptotic index and the area of explants of test and control groups at the first and final day of the experiment were compared by t-test. There was no difference in tail tip area between test and control groups, but a significantly higher (p<0.01) index of apoptosis in explants of the test group. This data suggest that T3 10cH modifies the effect of T3 at pharmacological dose, opening new perspectives for further studies and investigation of the dose-effect curve.
  Homeopathy: the journal of the Faculty of Homeopathy 10/2011; 100(4):220-7. · 1.13 Impact Factor
• Article: Elimination of Escherichia coli from piglet carcases using an on-farm composting system.

  F R Martins Soto, C P Coelho, N R Benites, L V Bonamin, P A Melville
  The Veterinary record. 05/2010; 166(18):564-5.
• Article: A randomized controlled trial of homeopathic treatment of weaned piglets in a commercial swine herd.

  F R M Soto, E R Vuaden, C dP Coelho, N R Benites, L V Bonamin, S S de Azevedo
  [show abstract] [hide abstract]
  ABSTRACT: To evaluate the zootechnic performance and occurrence of diarrhea in piglets in the week post-weaning comparing supplementation with sucrose saline which contained or did not contain added homeopathic medicine. Animals were randomly divided into three groups of 24 piglets each. The control group did not receive any treatment. Another group received sucrose saline, and the third group received sucrose saline with homeopathic medicine added, in the period of zero to seven days post-weaning. The homeopathic treatment consisted of Echinacea angustifolia, Avena sativa, Ignatia amara, Calcarea carbonica, all 6cH. Piglets were weighed daily for weight gain or loss, and observed for diarrhea and feed intake. Animals receiving sucrose saline alone and sucrose saline with homeopathy had less weight loss than control (p=0.017, p=0.0001 respectively). There was no statistical difference in relation to overall incidence of diarrhea or food consumption. These data suggest that the supplementation with sucrose saline with added homeopathic medicine in the first seven days post-weaning may be an useful option to reduce weight loss in weaned piglets.
  Homeopathy: the journal of the Faculty of Homeopathy 11/2008; 97(4):202-5. · 1.13 Impact Factor
• Article: Therapeutic and pathogenetic animal models for Dolichos pruriens.

  C de Paula Coelho, V D'Almeida, M Pedrazzolli-Neto, C Duran-Filho, J C Florio, L M C Zincaglia, L V Bonamin
  [show abstract] [hide abstract]
  ABSTRACT: The therapeutic and pathogenetic effects of Dolichos pruriens were evaluated using experimental models in rats. In the therapeutic experiment Wistar rats were housed in a heated environment (25+/-3 degrees C) to induce itch, and treated with ascending potencies D. pruriens (6 cH, 9 cH, 12 cH and 30 cH), each for 10 days. The positive control group received vehicle (ethanol 30% in water). The negative control group received no treatment and were kept at a standard temperature. In the pathogenetic experiment, all animals were kept at a temperature of 20+/-3 degrees C and treated for 30 consecutive days with D. pruriens 6 or 30 cH, or ethanol vehicle, or no treatment. The experiments were performed blind. The statistical analysis used Bartlett's test, followed by ANOVA/Tuckey-Krammer or Kruskal-Wallis/Dunn. The results point to the existence of therapeutic effects, with inhibition of the itching, skin lesions and fur thinning produced by heat, more evident in later observations, with the 9 12, and 30 cH potencies (Kruskal-Wallis/Dunn; P=0.001). No changes were observed in the other parameters, such as open field activity and laterality of the itching. In the pathogenetic experiment, no changes were observed in any parameters examined. We conclude that the proposed experimental model demonstrates the therapeutic effect of D. pruriens, but not its pathogenetic effects.
  Homeopathy 08/2006; 95(3):136-43. · 1.14 Impact Factor
• Source

  Available from: Fabio Perazzo

  Article: Effect of Atropa belladonna and Echinacea angustifolia in homeopathic dilution on experimental peritonitis.

  C M V Pedalino, F F Perazzo, J C T Carvalho, K S Martinho, C de O Massoco, L V Bonamin
  [show abstract] [hide abstract]
  ABSTRACT: Atropa belladonna and Echinacea angustifolia have been used in homeopathy as modulators of inflammatory processes, in simple potency or 'accord of potencies', as recommended by homotoxicology. We evaluated their effects on leukocyte migration and macrophage activity induced by experimental peritonitis in vivo. Mice were injected (i.p.) with LPS (1.0mg/kg) and treated (0.3ml/10g/day, s.c.) with different commercial forms of these medicines. Echinacea angustifolia D4--a simple potency preparation--and Belladonna Homaccord, Belladonna Injeel, Belladonna Injeel Forte, Echinacea Injeel and Echinacea Injeel Forte--all in 'accord of potencies'--were tested. The association of A. belladonna and E. angustifolia in 'accord of potencies' produced an increase of polymorphonuclear cell migration (Kruskal-Wallis, P = 0.03) and a decrease of mononuclear cell percentages (Kruskal-Wallis, P < or = 0.04), when compared with control, mainly in preparations containing low potencies. The proportion of degenerate leukocytes was lower in the treated groups, compared to a control group (P < or = 0.05). The treated groups showed increased phagocytosis (P < or = 0.05), mainly in preparations containing high potencies. Our results suggest that A. belladonna and E. angustifolia, when prepared in 'accord of potencies', modulate peritoneal inflammatory reaction and have a cytoprotective action on leukocytes.
  Homeopathy 10/2004; 93(4):193-8. · 1.14 Impact Factor
• Article: Long-term accumulation and microdistribution of uranium in the bone and marrow of beagle dog.

  J D T Arruda-Neto, M V Manso Guevara, G P Nogueira, I D Taricano, M Saiki, C B Zamboni, L V Bonamin, S P Camargo, A C Cestari, A Deppman, F Garcia, A N Gouveia, F Guzman, O A M Helene, S A C Jorge, V P Likhachev, M N Martins, J Mesa, O Rodriguez, V R Vanin
  [show abstract] [hide abstract]
  ABSTRACT: The accumulation and microdistribution of uranium in the bone and marrow of Beagle dogs were determined by both neutron activation and neutron-fission analysis. The experiment started immediately after the weaning period, lasting till maturity. Two animal groups were fed daily with uranyl nitrate at concentrations of 20 and 100 microg g(-1) food. Of the two measuring techniques, uranium accumulated along the marrow as much as in the bone, contrary to the results obtained with single, acute doses. The role played by this finding for the evaluation of radiobiological long-term risks is discussed. It was demonstrated, by means of a biokinetical approach, that the long-term accumulation of uranium in bone and marrow could be described by a piling up of single dose daily incorporation.
  International Journal of Radiation Biology 09/2004; 80(8):567-75. · 2.28 Impact Factor
• Source

  Available from: Fabio Perazzo

  Article: Action of Causticum in inflammatory models.

  J de Araújo Prado Neto, F F Perazzo, L G V Cardoso, L V Bonamin, J C Tavares Carvalho
  [show abstract] [hide abstract]
  ABSTRACT: The anti-inflammatory effect of Causticum was evaluated using acute and chronic inflammatory models in vivo. The administration of concentrated Causticum solution into the hind paw of rats produced an inflammatory reaction with oedema formation within the first hour, showing that Causticum acts as an oedematogenic agent. Carrageenin induced rat paw oedema was significantly inhibited (P<0.05) in the group treated with Causticum 30cH solution compared to control. Groups treated with potentized Causticum (6cH, 12cH, 30cH and 200cH), showed significant inhibition (P<0.05) of the inflammation pre-induced by carrageenin. However pre-treatment with Causticum 30cH for 6 days (0.5 ml, daily) did not significantly inhibit granulation using an implantation method.
  Homeopathy 01/2004; 93(1):12-6. · 1.14 Impact Factor
• Source

  Available from: altcancerweb.com

  Article: Very high dilutions of dexamethasone inhibit its pharmacological effects in vivo.

  L V Bonamin, K S Martinho, A L Nina, F Caviglia, R G Do Rio
  [show abstract] [hide abstract]
  ABSTRACT: We evaluated the interaction of dexamethasone 10(-17) and 10(-33) M (equivalent to 7cH and 15cH) with dexamethasone in pharmacological concentrations, using as experimental models: acute inflammation induced by carrageenan, Ehrlich ascitic tumour, and migration of tumour infiltrating leukocytes (TIL). Male adult BALB/c mice (n=7 per group) were used in all experiments. Carrageenan (1%) was injected into the footpad for oedema evaluation and into the peritoneal cavity (i.p.), for differential counting of inflammatory cells. Ehrlich ascitic tumour cells (10(7) viable cells/ml) were injected i.p. and tumour cells were counted after 6 days, by the Trypan blue exclusion method. The differential TIL was counted using smears stained by hematoxylin-eosin. Treatments were made immediately after carrageenan inoculation or once a day, during Ehrlich tumour development, until the animals were killed. Animals were treated with the following preparations: (1) phosphate buffer saline (PBS) solution; (2) dexamethasone (0.5 mg/kg for inflammation model or 4mg/kg for tumour model) mixed with dexamethasone 7cH or 15cH; (3) dexamethasone (same doses) mixed in PBS. Homeopathic dexamethasone partially blocked the anti-inflammatory effect of pharmacological dexamethasone with regard to paw oedema (two-way ANOVA, P < 0.0008) and polymorphonuclear cell migration (chi2, P=0.0001). No important differences were observed between experimental and control groups, in relation to Ehrlich tumour cells viability or count, or bodyweight, but potentised dexamethasone restored control levels of TIL viability, compared to mice treated with pharmacological doses of dexamethasone (chi2, P< or = 0.001). The results demonstrate that a potentised substance may change its own pharmacological effects and suggest that ultradilutions effects act mostly on host response.
  British Homoeopathic journal 11/2001; 90(4):198-203.
• Article: Effect of a submaxillary gland extract on Ehrlich tumor growth in mice.

  P Weill, R Frussa-Filho, L V Bonamin
  [show abstract] [hide abstract]
  ABSTRACT: Ablation of host submaxillary glands modifies Ehrlich tumor growth and tumor-infiltrating leukocytes, possibly by modifications in the serum level of growth factors produced by this gland. To extend this research, 7-month-old male EPM-1 mice (N = 30) were divided into two groups: 1) inoculated with tumor cells previously incubated with submaxillary salivary gland extract (SGE) in PBS for 30 min at 37%; 2) inoculated with tumor cells previously incubated with PBS, under the same conditions. Animals were inoculated into the footpad with 40 microl of a suspension containing 4.5 x 10(7) tumor cells/ml, and footpad thickness was measured daily for 10 days. Sections and smears of tumor cells were prepared from the tumor mass to determine mitosis frequency, percent of tumor cells immunopositive to nerve (NGF) and epidermal (EGF) growth factors and percent of tumor-infiltrating leukocytes. The incubation of tumor cells with SGE produced a tumor reduction of about 30% in size (P<0.01). This effect was not related to loss of cell viability during incubation, but a 33% increase (P<0.05) in the percentage of dead or dying tumor cells and a 15% increase in the percent of NGF/EGF-positive tumor cells (P<0.01) were observed in vivo at the end of experiment. Tumor-infiltrating lymphocytes and mitosis frequency did not differ between groups. These data suggest a direct effect of factors present in SGE on tumor cells, which induce degeneration of tumor cells.
  Brazilian Journal of Medical and Biological Research 11/1999; 32(10):1205-9. · 1.13 Impact Factor
• Article: Substance P does not modify mononuclear cell migration into Ehrlich tumor mass.

  L V Bonamin, B E Malucelli
  [show abstract] [hide abstract]
  ABSTRACT: In the present study, seven adult male mice were inoculated with Ehrlich tumor into the footpad after local substance P release was blocked by neurectomy of the sciatic and saphenous nerves. The contralateral footpad was also inoculated but sham-operated, and used as control. This procedure did not modify the percent of CD4+ (about 1-2%), CD8+ (about 1-3%), macrophages (about 21-22%), lymphocyte B (about 0-1%) and NK (about 1-2%) mononuclear cells present among tumor cells. These data suggest that chemotactic activity of substance P may not be relevant in this situation because the lack of this neurotransmitter (checked by immunohistochemistry) secondary to neurectomy did not change the cell migration profile into tumor mass.
  Brazilian Journal of Medical and Biological Research 04/1996; 29(3):359-62. · 1.13 Impact Factor
• Article: The influence of social isolation and peripheral innervation on Ehrlich tumor growth in mice.

  L V Bonamin, B E Malucelli
  [show abstract] [hide abstract]
  ABSTRACT: The relationship between social isolation and Ehrlich tumor growth was investigated in seven male NIH mice about 2 months old living separately in small cages for 28 days. Fifteen control animals were kept grouped in conventional cages (10 animals in one and 5 in another) for the same period. After this period, 40 to 50 microliters of a cell tumor suspension at a concentration of 1 to 5 x 10(7) tumor cells/ml was inoculated into the footpad and footpad size was measured for 10 days. Isolated mice presented a 30% reduction in tumor growth. Sciatic and saphenous neurectomy in one leg of 5 isolated mice (experimental) and 5 grouped mice (control) performed 7 days before tumor inoculation abolished this difference, and more tumor growth was observed in the neurectomized paw compared to the non-neurectomized paw. The subordinate x dominant social relationship established between mice living in groups of two per cage (seven cages) did not modify the growth of tumor inoculated under the same conditions compared to the first experiment. We conclude that social isolation and an intact peripheral innervation are associated with reduced tumor growth, but dominance behavior has no effect.
  Brazilian Journal of Medical and Biological Research 06/1995; 28(5):557-62. · 1.13 Impact Factor