S L Yang

Publications (2)3.66 Total impact

• Article: Sequence characterization and promoter identification of porcine APC10 gene.

  W B He, Z W Wang, Y Li, Z L Tang, S L Yang, Y L Mu, K M Peng, K Li
  [show abstract] [hide abstract]
  ABSTRACT: APC10 protein, a subunit of the anaphase-promoting complex (APC), plays an essential role in the progression of cells from mitosis to G1. In this study, we cloned and sequenced partial cDNA, intron 1 and 5'-flanking sequences of porcine APC10. The partial cDNA is 595 bp long and has an open reading frame of 558 bp which encodes 185 putative amino acids. Real-time PCR analysis revealed that the porcine APC10 mRNA expression shows a wide distribution and expression levels varies within a small different range in detected tissues. The deduced protein has a high identity with other eight species and comprises a conserved DOC domain. The phylogenetic tree indicated that porcine APC10 has the closest genetic relationship with human, monkey and dog. Promoter activity was demonstrated by transient transfection of 5'-deletion promoter/luciferase constructs into PK15 cells, and indicated that the proximal region from -2,052 to -1,764 is necessary for basal promoter activity. Positive cis-regulatory elements are present from -2,544 to -2,052 and from -3,114 to -2,774, while negative cis-regulatory elements may be present from -2,774 to -2,544. Yeast one-hybrid assay revealed Sp1 can interact with proximal promoter region of porcine APC10.
  Molecular Biology Reports 03/2010; 37(8):3841-9. · 2.93 Impact Factor
• Article: New mutations in growth hormone and receptor genes from Chinese Wuzhishan miniature pig

  J.T. Li, Y.L. Mu, L. Zhang, S.L. Yang, K. Li, S.T. Feng
  Acta Agriculturae Scandinavica A 06/2007; 57(2):97-100. · 0.73 Impact Factor