Sangdun Choi

Publications (3)36.51 Total impact

• Source

  Available from: Rod A Wing

  Article: The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome.

  Fred S Dietrich, Sylvia Voegeli, Sophie Brachat, Anita Lerch, Krista Gates, Sabine Steiner, Christine Mohr, Rainer Pöhlmann, Philippe Luedi, Sangdun Choi, Rod A Wing, Albert Flavier, Thomas D Gaffney, Peter Philippsen
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  ABSTRACT: We have sequenced and annotated the genome of the filamentous ascomycete Ashbya gossypii. With a size of only 9.2 megabases, encoding 4718 protein-coding genes, it is the smallest genome of a free-living eukaryote yet characterized. More than 90% of A. gossypii genes show both homology and a particular pattern of synteny with Saccharomyces cerevisiae. Analysis of this pattern revealed 300 inversions and translocations that have occurred since divergence of these two species. It also provided compelling evidence that the evolution of S. cerevisiae included a whole genome duplication or fusion of two related species and showed, through inferred ancient gene orders, which of the duplicated genes lost one copy and which retained both copies.
  Science 05/2004; 304(5668):304-7. · 31.20 Impact Factor
• Article: Construction and characterization of two rice bacterial artificial chromosome libraries from the parents of a permanent recombinant inbred mapping population

  Hong-Bin Zhang, Sangdun Choi, Sung-Sick Woo, Zhikang Li, Rod A. Wing
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  ABSTRACT: Rice is a leading grain crop and the staple food for over half of the world population. Rice is also an ideal species for genetic and biological studies of cereal crops and other monocotyledonous plants because of its small genome and well developed genetic system. To facilitate rice genome analysis leading to physical mapping, the identification of molecular markers closely linked to economic traits, and map-based cloning, we have constructed two rice bacterial artificial chromosome (BAC) libraries from the parents of a permanent mapping population (Lemont and Teqing) consisting of 400 F9 recombinant inbred lines (RILs). Lemont (japonica) and Teqing (indica) represent the two major genomes of cultivated rice, both are leading commercial varieties and widely used germplasm in rice breeding programs. The Lemont library contains 7296 clones with an average insert size of 150 kb, which represents 2.6 rice haploid genome equivalents. The Teqing library contains 14208 clones with an average insert size of 130 kb, which represents 4.4. rice haploid genome equivalents. Three single-copy DNA probes were used to screen the libraries and at least two overlapping BAC clones were isolated with each probe from each library, ranging from 45 to 260 kb in insert size. Hybridization of BAC clones with chloroplast DNA probes and fluorescent in situ hybridization using BAC DNA as probes demonstrated that both libraries contain very few clones of chloroplast DNA origin and are likely free of chimeric clones. These data indicate that both BAC libraries should be suitable for map-based cloning of rice genes and physical mapping of the rice genome.
  Molecular Breeding 01/1996; 2(1):11-24. · 2.85 Impact Factor
• Article: Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

  Sangdun Choi, Robert A. Creelman, John E. Mullet, Rod A. Wing
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  ABSTRACT: We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.
  Plant Molecular Biology Reporter 05/1995; 13(2):124-128. · 2.45 Impact Factor