S Kobayashi

National Livestock Breeding Center, Hukusima, Fukushima, Japan

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Publications (802)1108.63 Total impact

  • Review of Scientific Instruments 02/2015; 86(2):023502. · 1.58 Impact Factor
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    ABSTRACT: We demonstrated that in vivo-matured oocytes (mOC) collected by ovum-pick up (OPU) from cows after stimulation of follicular growth (FG) are suitable for producing good quality blastocysts (BL). However, it is not known whether duration of FG affects developmental competence of mOC. The purpose of this study was to examine development of mOC after stimulation with different duration of FG. Japanese black donor cows (n=4 per each group), were treated with a CIDR at Day 0. Follicle of diameter >8mm were removed on Day 5. A total 20 AU of FSH was administrated to cows twice daily with decreasing doses from the evening of Day 6 to the morning of Day 10. In the conventional group (48PG), a administration of PGF2α (0.75mg of cloprostenol), CIDR withdrawal, and administration of GnRH (0.2mg of fertirelin acetate) were performed on the evening of Day 8, morning of Day 9, and morning of Day 10, respectively. In the experimental group (72PG), administration of PGF2a, CIDR withdrawal, and administration of GnRH were performed on the evening of Day 9, the morning of Day 10, and the morning of Day 11, respectively. The mOC were collected from follicles >5mm by OPU at 25 to 26h following GnRH administration. Collected mOC were inseminated with 3×10(6) spermmL(-1) in BO solution on 30h after GnRH. After 6h of IVF, presumptive zygotes were cultured for 168h in 5% CS+CR1aa, using a micro-well culture dish (Dai-Nippon-Print) and time-lapse cinematography (CCM-1.4MZS; Astec) for individual embryo observation. The kinetics of early embryo was analysis by CCM-1.4 software. To assess the quality of BL, prognostic factors were used as follows: (1) less than 27 hpi (hours post-insemination) at the first cleavage (1st CD), (2) 2 blastomeres at the end of 1st CD, and (3) absence of multiple fragments at the end of the 1st CD (Sugimura et al. 2012 PLoS ONE 7, e36627; Imai et al. 2014 Reprod. Fertil. Dev. 26, 182). Data were analysed by Student's t-test or chi-square test. The number of mOC were 12.5±4.7 and 10.3±2.7 (means ± s.e.) oocytes per session in 48PG and 72PG. There was no significant difference in cleavage rate or BL formation rate (97.5±1.5 v. 98.2±1.8%, 66.3±8.2 v. 66.8±3.5%, respectively). The time for 1st CD was shorter in 48PG (26.1±0.3 v. 27.8±0.4; P<0.01), and the rate of 1st CD less than 27 hpi was superior in 48PG compared with 72PG (74.3 v. 42.9%; P<0.05). However, the rate of 2 blastomeres and absence of multiple fragments were not different between 48PG and 72PG. The number of BL tended to decrease in 72PG compared with 48PG (28.6 v. 48.6%; P=0.087). These results indicate that duration of FG did not affect the rate of cleavage and BL formation. However, extension of duration of FG might reduce the quality of BL.
    Reproduction Fertility and Development 12/2014; 27(1):207. · 2.58 Impact Factor
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    ABSTRACT: In order to evaluate the electron cyclotron (EC) heating power inside the Large Helical Device vacuum vessel and to investigate the physics of the interaction between the EC beam and the plasma, a direct measurement system for the EC beam transmitted through the plasma column was developed. The system consists of an EC beam target plate, which is made of isotropic graphite and faces against the EC beam through the plasma, and an IR camera for measuring the target plate temperature increase by the transmitted EC beam. This system is applicable to the high magnetic field (up to 2.75 T) and plasma density (up to 0.8 × 10(19) m(-3)). This system successfully evaluated the transmitted EC beam profile and the refraction.
    Review of Scientific Instruments 11/2014; 85(11):11E822. · 1.58 Impact Factor
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    ABSTRACT: A new type monitor of power and polarization states of millimeter-waves has been developed to be installed at a miter-bend, which is a part of transmission lines of millimeter-waves, for electron cyclotron resonance heating on the Large Helical Device. The monitor measures amplitudes and phase difference of the electric field of the two orthogonal polarizations which are needed for calculation of the power and polarization states of waves. The power and phase differences of two orthogonal polarizations were successfully detected simultaneously.
    Review of Scientific Instruments 11/2014; 85(11):11D831. · 1.58 Impact Factor
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    ABSTRACT: A fluctuation analysis technique using analytic signals is proposed. Analytic signals are suitable to characterize a single mode with time-dependent amplitude and frequency, such as an MHD mode observed in fusion plasmas since the technique can evaluate amplitude and frequency at a specific moment without limitations of temporal and frequency resolutions, which is problematic in Fourier-based analyses. Moreover, a concept of instantaneous phase difference is newly introduced, and error of the evaluated phase difference and its error reduction techniques using conditional/ensemble averaging are discussed. These techniques are applied to experimental data of the beam emission spectroscopic measurement in the Heliotron J device, which demonstrates that the technique can describe nonlinear evolution of MHD instabilities. This technique is widely applicable to other diagnostics having necessity to evaluate phase difference.
    Review of Scientific Instruments 10/2014; 85(11). · 1.58 Impact Factor
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    ABSTRACT: A control system for a fast steering mirror has been newly developed for the electron cyclotron heating (ECH) launchers in the large helical device. This system enables two-dimensional scan during a plasma discharge and provides a simple feedback control function. A board mounted with a field programmable gate array chip has been designed to realize feedback control of the ECH beam position to maintain higher electron temperature by ECH. The heating position is determined by a plasma diagnostic signal related to the electron temperature such as electron cyclotron emission and Thomson scattering.
    Review of Scientific Instruments 10/2014; 85(11). · 1.58 Impact Factor
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    ABSTRACT: A Nd:YAG Thomson scattering system has been developed for Heliotron J. The system consists of two 550 mJ 50 Hz lasers, large collection optics, and 25 radial channel (̃1 cm spatial resolution) interference polychromators. This measurement system achieves a S/N ratio of ̃50 for low-density plasma (ne ̃ 0.5 × 1019 m-3). A time evolution of electron temperature profiles was measured with this system for a high-intensity gas-puff (HIGP) fueling neutral-beam-injection plasma. The peripheral temperature of the higher-density phase after HIGP recovers to the low-density pre-HIGP level, suggesting that improving particle transport in the HIGP plasma may be possible.
    Review of Scientific Instruments 10/2014; 85(11). · 1.58 Impact Factor
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    ABSTRACT: Enhancement of the output power per gyrotron has been planned in the Large Helical Device (LHD). Three 77-GHz gyrotrons with an output power of more than 1 MW have been operated. In addition, a high power gyrotron with the frequency of 154 GHz (1 MW/5 s, 0.5 MW/CW) was newly installed in 2012, and the total injection power of Electron cyclotron resonance heating (ECRH) reached 4.6 MW. The operational regime of ECRH plasma on the LHD has been extended due to the upgraded ECRH system such as the central electron temperature of 13.5 keV with the line-averaged electron density n e_fir = 1 × 1019 m−3. The electron thermal confinement clearly improved inside the electron internal transport barrier, and the electron thermal diffusivity reached neoclassical level. The global energy confinement time increased with increase of n e_fir. The plasma stored energy of 530 kJ with n e_fir = 3.2 × 1019 m−3, which is 1.7 times larger than the previous record in the ECRH plasma in the LHD, has been successfully achieved.
    Physics of Plasmas 06/2014; 21(6):061506. · 2.25 Impact Factor
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    ABSTRACT: A novel reconstruction method is developed for acquiring the electron density profile from multi-channel interferometric measurements of strongly asymmetrical toroidal plasmas. It is based on a regularization technique, and a generalized cross-validation function is used to optimize the regularization parameter with the aid of singular value decomposition. The feasibility of method could be testified by simulated measurements based on a magnetic configuration of the flexible helical-axis heliotron device, Heliotron J, which has an asymmetrical poloidal cross section. And the successful reconstruction makes possible to construct a multi-channel Far-infrared laser interferometry on this device. The advantages of this method are demonstrated by comparison with a conventional method. The factors which may affect the accuracy of the results are investigated, and an error analysis is carried out. Based on the obtained results, the proposed method is highly promising for accurately reconstructing the electron density in the asymmetrical toroidal plasma.
    Review of Scientific Instruments 05/2014; 85(5):053506-053506-7. · 1.58 Impact Factor
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    ABSTRACT: Nanostructured tungsten (W)-based materials offer many advantages for use as plasma facing materials and components exposed to heavy thermal loads combined with irradiation with high-energy neutron and low-energy ion. This paper first presents the recent progress in nanostructured toughened, fine grained, recrystallized W materials. Thermal desorption spectrometry apparatus equipped with an ion gun has been installed in the radiation controlled area in our Center at Tohoku University to systematically investigate the effects of displacement damage due to high-energy neutron irradiation on hydrogen isotope retention in connection with the nano- or micro-structures in W-based materials. In this paper, the effects of high-energy heavy ion irradiation on deuterium retention in W with different microstructures are described as a preliminary work with the prospective view of neutron irradiation effects.
    Physica Scripta 04/2014; 2014(T159):014032. · 1.03 Impact Factor
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    ABSTRACT: Edge fluctuation in a supersonic molecular-beam injection (SMBI) fueled plasma has been measured using an electrostatic probe array. After SMBI, the plasma stored energy (Wp) temporarily decreased then started to increase. The local plasma fluctuation and fluctuation induced particle transport before and after SMBI have been analyzed. In a short duration (̃4 ms) just after SMBI, the density fluctuation of broad-band low frequency increased, and the probability density function (PDF) changed from a nearly Gaussian to a positively skewed non-Gaussian one. This suggests that intermittent structures were produced due to SMBI. Also the fluctuation induced particle transport was greatly enhanced during this short duration. About 4 ms after SMBI, the low frequency broad-band density fluctuation decreased, and the PDF returned to a nearly Gaussian shape. Also the fluctuation induced particle transport was reduced. Compared with conventional gas puff, Wp degradation window is very short due to the short injection period of SMBI. After this short degradation window, fluctuation induced particle transport was reduced and Wp started the climbing phase. Therefore, the short period of the influence to the edge fluctuation might be an advantage of this novel fueling technique. On the other hand, although their roles are not identified at present, coherent MHD modes are also suppressed as well by the application of SMBI. These MHD modes are thought to be de-exited due to a sudden change of the edge density and/or excitation conditions.
    Physics of Plasmas 03/2014; 21(4). · 2.25 Impact Factor
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    ABSTRACT: This study investigated correlation of Mg# and Th abundance on the lunar highland to understand solidification and composition of the lunar magma ocean.
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    ABSTRACT: The interleukin (IL)-1 receptor antagonist (Ra) binds to IL-1 receptors and inhibits IL-1 activity. However, it is unclear whether IL-1Ra plays a protective role in periodontal disease. This study was undertaken to compare experimental periodontitis induced by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) in IL-1Ra knockout (KO) mice vs wild type (WT) mice. Computed tomography (CT) analysis, and hematoxylin eosin (H&E) and tartrate resistant acid phosphatase (TRAP) staining were performed. In addition, osteoblasts were isolated and related gene mRNA expression was assessed by real-time quantitative PCR (qPCR), calcification was detected by Alizarin red staining. Infected IL-1Ra KO mice exhibited elevated (P<0.05): antibody levels against A. actinomycetemcomitans, bone loss in furcation areas and alveolar fenestrations. Moreover, mRNA for tumor necrosis factor α (TNF-α), IL-6, macrophage colony-stimulating factor (M-CSF), and receptor activator of NF-kappaB ligand (RANKL) in IL-1Ra KO mice osteoblasts stimulated with A. actinomycetemcomitans were increased (P<0.05) compared to WT mice. Alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), and runt-related gene 2 (Runx2) mRNA were decreased (P<0.05). IL-1α mRNA expression was increased and calcification was not observed in IL-1 Ra KO mice osteoblasts. In brief, IL-1Ra deficiency promoted inflammatory cytokines beyond IL-1, and altered expression of genes involved in bone resorption in A. actinomycetemcomitans infected osteoblasts. Alterations consistent with rapid bone loss in infected IL-Ra KO mice were also observed in genes expressed in bone formation and calcification. In short, these data suggest that an IL-1Ra may serve as a potential therapeutic drug for periodontal disease.
    Infection and immunity 02/2014; · 4.16 Impact Factor
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    ABSTRACT: A collective Thomson scattering (CTS) diagnostic was developed and used to measure the bulk and fast ions originating from 180 keV neutral beams in the Large Helical Device (LHD). Electromagnetic waves from a gyrotron at 77 GHz with 1 MW power output function as both the probe and electron cyclotron heating beam. To clarify the diagnostic applicability of the gyrotron in the 77 GHz frequency band, we investigated the dependence of the probe and receiver beam trajectories in plasmas with high electron densities of (4–5) × 10 19 m −3 and low electron densities of (1–2) × 10 19 m −3 . At high density, a stray radiation component was observed in the CTS spectrum whereas it was negligibly small at low density. The CTS spectrum was measured and analysed after the in situ beam alignment using a beam scan. Qualitatively, the CTS spectrogram shows consistent response to ion temperatures of 1–2 keV for electron densities of (1–2) × 10 19 m −3 and electron temperatures of 2–4 keV. The measured CTS spectrum shows an asymmetric shape at the foot of the bulk-ion region during the injection of 180 keV fast ions. This shape is explained by the fast-ion distribution in the velocity space (v || , v ⊥) based on Monte Carlo simulation results. The analysis method of the CTS spectra is used to evaluate the ion temperature and fast-ion velocity distribution from the measured CTS data.
    01/2014; 54(02):023006.
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    ABSTRACT: The effect of neutron flux on magnetic minor hysteresis loops has been investigated on nuclear reactor pressure vessel steels, which were irradiated to a fluence of $3.3times 10^{19}~{rm n}/{rm cm}^{2}$ . A minor-loop coefficient, which is an indicator of internal stress, exhibits a local maximum at a fluence of ${sim} 1times 10^{19}~{rm n}/{rm cm}^{2}$ , whose position shifts to a low-fluence regime with decreasing neutron flux. Introducing an effective fluence, used to correct the flux effect of irradiation hardening, the data obtained by different flux were found to almost fall on single curve for some alloys. This implies that the flux effect on magnetic property is dominated by efficiency of radiation-enhanced diffusion of solute atoms, such as Cu, as in the case of irradiation hardening.
    IEEE Transactions on Magnetics 01/2014; 50(4):1-4. · 1.21 Impact Factor
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    ABSTRACT: Recently, we reported on a promising system for selecting healthy IVF embryos in cattle using kinetics of early embryo development and oxygen consumption of blastocyst [Sugimura et al. 2012 PLoS ONE 7, e36627]. The present study was conducted to examine the differences in embryo quality of bovine blastocysts obtained after IVF of in vivo-matured oocytes with X-sorted and unsorted sperm. Holstein dry cows (n=8) were reared under the same feeding and environmental conditions. Two ovum pickup (OPU) sessions were conducted in each cow to fertilize with or without X-sorted sperm. In vivo-matured oocytes were collected by OPU just before ovulation after superstimulation treatment. The oocytes were inseminated with 5×10(6) spermmL(-1) of each sperm, and presumptive zygotes were cultured in CR1aa supplemented with 5% newborn calf serum and 0.25mgmL(-1) of linolenic acid albumin at 38.5 C in 5% CO2, 5% O2, and 90% N2 for 168h. Embryo kinetics were observed individually using a microwell culture dish (Dai-Nippon Print) and time-lapse cinematography (CCM-1.4MZS; Astec, Fukuoka, Japan; Sugimura et al. 2010 Biol. Reprod. 83, 970-978). Photographs of each embryo were taken every 15min during the in vitro culture period and images were analysed by CCM-1.4 software (Astec). By assessing the quality of blastocysts, a combination of identified prognostic factors were used: (1) timing of the first cleavage (less than 27h post-insemination); (2) two blastomeres at the end of the first cleavage; (3) absence of fragments at the end of the first cleavage; and (4) six or more blastomeres at the onset of the lag-phase. Data were analysed by ANOVA. In total, 34.1±18.4 oocytes per session per donor were collected by OPU, and 23.7±13.4 oocytes had an expanded cumulus cell. Oocyte recovery rates were recorded at 77.1±15.1%. After IVF and in vitro culture, 10.6±7.7 blastocysts per session per donor were produced in this study. There was no significantly difference in cleavage rates and blastocyst formation rates between X-sorted sperm and unsorted sperm (87.1±10.8 and 82.6±12.1% and 38.4±23.6 and 57.1±23.4%, respectively). However, blastocysts derived from X-sorted sperm showed significantly (P<0.05) lower quality in the prognostic factor (1) and combined (1) to (4) than that in unsorted sperm (35.3 v. 54.0 and 14.7 v. 42.9%, respectively). Pregnancy rates were higher for the blastocysts that had a high score in the prognostic factors (1) to (4) compared to those that had a low score (75.0%, n=8 v. 36.4%, n=22). These results suggest that quality of blastocysts, based on the prognostic factors studied, derived from X-sorted sperm is lower than that from unsorted sperm.
    Reproduction Fertility and Development 12/2013; 26(1):182. · 2.58 Impact Factor
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    ABSTRACT: Previously, it was reported that a high rate of good quality blastocysts were produced by IVF of in vivo-matured oocytes, obtained by ovum pick up (OPU) after superstimulation in Holstein cows, using X-sorted sperm (Matoba et al. 2012 Reprod. Domest. Anim. 47, 515). In this system, an early first cleavage within 28h after IVF was found to be a potent marker for the selection of embryos with high developmental competence (Matoba et al. 2013 Reprod. Fertil. Dev. 25, 266). However, we have limited knowledge on the timing and normality of embryonic cleavages in in vitro-matured oocytes after IVF. The purpose of the present study was to compare the kinetics and patterns of the first cleavage of in vivo- and in vitro-matured bovine oocytes after IVF with X-sorted sperm. In vivo-matured oocytes (Group A) were collected by OPU from non-lactating Holstein cows just before ovulation after superstimulation. Immature oocytes were either collected by OPU without hormonal treatment or by aspiration of ovaries at the local abattoir and matured in vitro (Group B or C). All the oocytes were inseminated with 5×10(6)spermmL(-1) of X-sorted sperm, except half of oocytes in Group C inseminated by non-sorted sperm (Group D) and cultured in CR1aa supplemented with 5% calf serum and 0.25mgmL(-1) of linoleic acid albumin at 38.5°C in 5% CO2, 5% O2, and 90% N2 for 216h. Embryo kinetics were observed individually using a microwell culture dish and time-lapse cinematography (Sugimura et al. 2010 Biol. Reprod. 83, 970-978). Photographs of each embryo were taken in every 15min during the IVC period and analysed by time-lapse cinematography software. Cleavage pattern was categorized as normal (2 even blastomeres without fragment or protrusion) or abnormal (2 uneven blastomeres, with fragment or protrusion and those dividing into 3-5 blastomeres) at the first cleavage. Data were analysed by ANOVA, chi-squared, or discriminant function. A total of 268 cleaved embryos were used. The blastocyst rate in Group A was higher than in Groups B and C (61.3 v. 40.0 and 25.0%, respectively; P<0.05). The timing of first cleavage was longer in Group A compared with Groups C and D (28.3±3.8 v. 27.6±3.8 and 26.7±1.9h, respectively) and in Group B (28.1±4.0h) compared with in Group D (P<0.05). Higher rates of normal cleavage were observed in Groups A, B, and D than in Group C (53.5, 44.4, and 54.8 v. 16.7%, respectively; P<0.01). The frequency of blastocysts derived from the early (28.3h) and normal pattern cleaving oocytes were greater in Groups A and B than in Group C (29.0 and 20.0 v. 8.3%, respectively; P<0.05) and similar in Group D (22.6%). Our results reveal that IVF embryos produced from in vivo-matured oocytes with sex-sorted sperm had superior normality than those produced from in vitro-matured oocytes and similar normality to embryos inseminated with non-sorted sperm.
    Reproduction Fertility and Development 12/2013; 26(1):182-3. · 2.58 Impact Factor
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    ABSTRACT: Results of three magnetic nondestructive methods, Magnetic Barkhausen Emission (MBE), magnetic minor loops Power Scaling Laws (PSL) and Magnetic Adaptive Testing (MAT), and of one reference mechanical measurement, Vickers Hardness (HV), applied on the same series of neutron heavily irradiated nuclear reactor pressure vessel steel materials, were normalized and presented here for the purpose of their straightforward quantitative mutual comparison. It is uncommon to carry out different round-robin testing on irradiated materials, and if not answering all open questions, the comparison alone justifies this paper. The assessment methods were all based on ferromagnetism, although each of them used a different aspect of it. The presented comparison yielded a justified recommendation of the most reliable nondestructive method for indication of the reactor steel irradiation hardening and embrittlement effects. The A533 type B Class 1 steel (JRQ), and the base (15Kh2MFA) and welding (10KhMFT) steels for the WWER 440-type Russian reactors were used for the investigations. The samples were irradiated by high-energy neutrons (>1 MeV) with up to 11.9 × 1019 n/cm2 fluences. From all the applied measurements, the results of MAT produced the most satisfactory correlation with independently measured ductile-brittle-transition temperature (DBTT) values of the steel. The other two magnetic methods showed a weaker correlation with DBTT, but some other aspects and information could be assessed by them. As MAT and MBE were sensitive to uncontrolled fluctuation of surface quality of the steel, contact-less ways of testing and more conveniently shaped irradiated nuclear pressure vessel steel samples were suggested for future measurements.
    Nuclear Engineering and Design 12/2013; 265:201–209. · 0.97 Impact Factor
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    ABSTRACT: Second harmonic electron cyclotron current drive (ECCD) has been applied in the stellarator/heliotron (S/H) device, Heliotron J, to stabilize magnetohydrodynamic (MHD) modes. The energetic particle mode (EPM) of 60-90 kHz frequency, one of the energetic-ion-driven MHD modes, is excited in a plasma heated by co- and counter-neutral beam injection and electron cyclotron heating (ECH). The EPM has been stabilized by counter-ECCD which decreases the rotational transform. Localized EC current driven by a few kA at the central region modifies the rotational transform profile, ι/2π, leading to the formation of a high magnetic shear at the radius where the mode is excited. An experiment scanning the EC-driven current shows that there is a threshold in magnetic shear and/or rotational transform to stabilize the EPM.
    Nuclear Fusion 11/2013; 53(11):3041-. · 3.24 Impact Factor
  • International Journal of Cardiology 10/2013; · 6.18 Impact Factor

Publication Stats

5k Citations
1,108.63 Total Impact Points


  • 2009–2014
    • National Livestock Breeding Center
      Hukusima, Fukushima, Japan
    • University of Toulouse
      Tolosa de Llenguadoc, Midi-Pyrénées, France
  • 2004–2014
    • Ehime University
      • Department of Materials Science and Engineering
      Matuyama, Ehime, Japan
    • Saitama Prefectural University
      Saitama, Saitama, Japan
  • 2001–2014
    • National Institute for Fusion Science
      • Department of Helical Plasma Research
      Tokitsu-chō, Gifu, Japan
    • Chiba University Hospital
      Tiba, Chiba, Japan
  • 1989–2014
    • Kyoto University
      • • Institute of Advanced Energy
      • • Graduate School of Energy Science
      Kioto, Kyōto, Japan
  • 2013
    • Aichi Gakuin University
      • Department of Periodontology
      Nagoya, Aichi, Japan
  • 2005–2013
    • Iwate University
      • Faculty of Engineering
      Morioka, Iwate, Japan
    • Barrow Neurological Institute
      • Department of Neurosurgery
      Phoenix, Arizona, United States
  • 2010–2012
    • Japan Aerospace Exploration Agency
      • Institute of Space and Astronautical Science (ISAS)
      Chōfu, Tōkyō, Japan
    • National Institute of Radiological Sciences
      Tiba, Chiba, Japan
    • Gunma University
      • Department of Chemistry
      Maebashi, Gunma Prefecture, Japan
  • 2006–2012
    • Ashikaga Institute of Technology
      Asikaga, Tochigi, Japan
  • 2004–2012
    • Saitama University
      • • Graduate School of Science and Engineering
      • • Faculty of Engineering
      Saitama, Saitama, Japan
  • 1998–2012
    • Nippon Medical School
      • Department of Neurosurgery
      Tokyo, Tokyo-to, Japan
    • Kokura Memorial Hospital
      Kitakyūshū, Fukuoka, Japan
  • 1989–2011
    • Saga University
      • Department of Physics
      Сага Япония, Saga, Japan
  • 1988–2011
    • Shimane University
      • • Department of Internal Medicine
      • • Faculty of Medicine
      Matsu, Shimane Prefecture, Japan
  • 2006–2010
    • Waseda University
      • Advanced Research Institute for Science and Engineering
      Edo, Tōkyō, Japan
  • 2008–2009
    • University of Fukui
      • Research Center for Development of Far-Infrared Region (FIR FU)
      Hukui, Fukui, Japan
    • Fukui University
      Hukui, Fukui, Japan
    • Kyushu Sangyo University
      Hukuoka, Fukuoka, Japan
    • Tokyo University of Science
      Edo, Tōkyō, Japan
  • 2002–2009
    • Science University of Tokyo, Yamaguchi
      Yamaguti, Yamaguchi, Japan
  • 1999–2009
    • University of Tsukuba
      • Applied Physics
      Tsukuba, Ibaraki, Japan
  • 1973–2007
    • The University of Tokyo
      • • Department of Physics
      • • Institute of Medical Science
      Tokyo, Tokyo-to, Japan
  • 2003
    • Tokyo Institute of Technology
      • Department of Physics
      Edo, Tōkyō, Japan
    • National Taiwan University
      • Department of Physics
      Taipei, Taipei, Taiwan
  • 2001–2003
    • High Energy Accelerator Research Organization
      • Institute of Particle and Nuclear Studies
      Tsukuba, Ibaraki, Japan
  • 1992–2003
    • Tohoku University
      • • Institute of Development, Aging and Cancer
      • • Department of Medical Genetics
  • 1969–2001
    • Shinshu University
      • Department of Neurosurgery
      Shonai, Nagano, Japan
  • 2000
    • National Institute for Agro-Environmental Sciences in Japan
      Tsukuba, Ibaraki, Japan
    • National Cancer Center, Japan
      Edo, Tōkyō, Japan
  • 1990–1999
    • North Internal Medicine
      Bartlett, Tennessee, United States
  • 1995–1997
    • Nara Hospital
      Ikuma, Nara, Japan
  • 1991–1994
    • Nippon Telegraph and Telephone
      Edo, Tōkyō, Japan
    • Tokyo University of Agriculture and Technology
      Edo, Tōkyō, Japan
    • Toshiba Corporation
      Edo, Tōkyō, Japan
    • National Defense Medical College
      • Division of Microbiology
      Tokorozawa, Saitama-ken, Japan
  • 1986–1992
    • Tokyo Junshin Women's College
      • • Institute of Gastroenterology
      • • Department of Microbiology
      Edo, Tōkyō, Japan
  • 1983
    • Rikkyo University
      • Department of Physics
      Edo, Tōkyō, Japan
  • 1981
    • Japan Red Cross Fukuoka Hospital
      Hukuoka, Fukuoka, Japan
  • 1980
    • Aichi Medical University
      • Department of Pharmacology
      Okazaki, Aichi, Japan
  • 1978–1980
    • Josai University
      Saitama, Saitama, Japan
  • 1966
    • Nagoya University
      • Division of of Internal Medicine
      Nagoya-shi, Aichi-ken, Japan