Publications (2)0 Total impact
Article: Approach to the large-scale preparation of highly pure phosphatidylserine from bovine brain.[show abstract] [hide abstract]
ABSTRACT: An approach to the large-scale preparation of highly pure phosphatidylserine from bovine brain is described in this paper. The method is based on (i) the separation of phosphatidylserine from phosphatidylinositol in bovine brain extract by preparative aminopropyl normal-phase high-performance liquid chromatography using methanol-1 M phosphoric acid (90:10, v/v) as mobile phase and (ii) further purification of phosphatidylserine by anion-exchange chromatography. The main advantage of this approach is that polyunsaturated acid-containing molecular species of brain phosphatidylserine are not lost in the preparation procedure.Journal of chromatography. B, Biomedical applications 05/1995; 666(1):178-82.
Article: Partial characterization of the molecular species of phosphatidylserine from human plasma by high-performance liquid chromatography and fast atom bombardment mass spectrometry.[show abstract] [hide abstract]
ABSTRACT: Phosphatidylserine from human plasma was purified and identified by amino normal-phase and octadecylsilyl reversed-phase high-performance liquid chromatography, respectively. The two major molecular species within the human plasma phosphatidylserine, qualitatively determined by negative-ion fast atom bombardment mass spectrometry, are 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine and 1-stearoyl-2-arachidonyl-sn-glycero-3-phosphoserine.Journal of chromatography. B, Biomedical applications 12/1994; 661(1):1-5.