Roberte Aubert

Paris Diderot University, Lutetia Parisorum, Île-de-France, France

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Publications (35)160.42 Total impact

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    ABSTRACT: Aims/IntroductionThe aim of the present study was to examine the associations of rs2241766 (+45T>G), rs1501299 (+276G>T), rs17300539 (−11391G>A) and rs182052 (−10069G>A) in the adiponectin (Ad) gene with adiponectin concentrations, and concomitantly the association of these variants with cardiometabolic risk in type 2 diabetic patients of African ancestry.Materials and MethodsA cross-sectional study of 200 patients was carried out. Concentrations of total, high (HMW), middle (MMW) and low (LMW) molecular weight adiponectin isoforms were measured. The four polymorphisms were genotyped.ResultsDecreased values were noted for total Ad in overweight, dyslipidemia and coronary artery disease (CAD), for HMW in overweight and dyslipidemia, for MMW in CAD, for LMW in dyslipidemia and CAD, for the percentage HMW/total in overweight, and for MMW:HMW ratio in patients without hypertriglyceridemic waist (HTGW). Significant associations were noted between total Ad, HMW, and HMW/total Ad and rs182052 under a dominant model (P = 0.04, P = 0.03 and P = 0.04, respectively), and between MMW and rs17300539 (P = 0.006). No significant difference in adiponectin concentrations was noted according to rs2241766 and rs1501299 genotypes. Patients carrying the rs2241766 G allele (TG+GG) had an increased risk of HTGW (odds ratio [OR] 3.1; P = 0.04) and of CAD (OR 3.3; P = 0.01). The odds of having low total adiponectin concentrations (<25th percentile: 3.49 ng/mL) for carrying the rs182052A allele (AA+GA) was: OR 0.40; P = 0.009. The single-nucleotide polymorphism associated with adiponectin levels was not concomitantly associated with cardiometabolic risk factors.Conclusions Adiponectin concentrations and ADIPOQ variants are implicated in the pathophysiological process leading to cardiovascular diseases, but the genetic effects seem to be independent of adiponectin concentrations in our Afro–Caribbean diabetic patients.
    03/2014; 5(2). DOI:10.1111/jdi.12133
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    ABSTRACT: Introduction L’adiponectine est associée à la sensibilité à l’insuline. Dans les études pangénomiques, les polymorphismes du gène de la t-cadhérine, CDH13, sont associés à l’adiponectinémie. La t-cadhérine est un récepteur pour les formes actives de l’adiponectine. Le but de notre étude est d’approfondir les relations entre variations génétiques de CDH13 et diabète de type 2 et paramètres associés. Patients et méthodes Quatre polymorphismes de CDH13 (rs11646213, rs12051272, rs3865188, rs4783244) ont été génotypés par méthode KASPAR dans une cohorte issue de la population générale, DESIR (N = 5 212), et une cohorte de sujets diabétiques de type 2, DIABHYCAR (N = 3 123). Les relations entre polymorphismes et variables quantitatives continues ont été estimées par ANCOVA, avec ajustement sur les facteurs confondants. Les relations des polymorphismes avec le risque de diabète de type 2 ont été estimés par Khi2, test de tendance (Cochrane) et régression logistique avec ajustement multiple. Résultats La distribution des génotypes CDH13 est différente entre sujets non diabétiques et diabétiques : les odds-ratios (95 % IC) de diabète de type 2 pour les variants mineurs sont 1,11 (1,04–1,19), 1,59 (1,06–2,38), 0,92 (0,87–0,99) et 0,91 (0,86–0,98) pour les SNP rs11646213, rs12051272, rs3865188 et rs4783244 respectivement. Le variant rs11646213, associé à une augmentation du risque de diabète de type 2, est associé également à un IMC augmenté (p = 0,04), une HbA1c augmentée (p = 0,006) et une adiponectinémie diminuée (p = 0,03) dans la population générale. Inversement, les variants rs3865188 et rs4783244, associés à une diminution du risque de diabète, sont également associés à un IMC diminué (p = 0,04), une HbA1c diminuée (p = 0,03) et une adiponectinémie augmentée (p = 0,002 et 0,003). Conclusion Les polymorphismes de CDH13 sont associés au diabète de type 2 dans la population française. Cette association pourrait être due à des effets sur l’IMC et l’adiponectinémie. Ces résultats peu décrits dans les populations européennes, doivent être répliqués.
    Diabetes & Metabolism 03/2014; 40:A9. DOI:10.1016/S1262-3636(14)72209-8 · 2.85 Impact Factor
  • Diabetes & Metabolism 03/2011; 37(1). DOI:10.1016/S1262-3636(11)70805-9 · 2.85 Impact Factor
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    ABSTRACT: High total adiponectin (ADPN) levels were reported in type 1 diabetes (T1D) and related to long diabetes duration and nephropathy. We studied whether ADPN and its specific isoforms were elevated in T1D without microangiopathy and whether they were related to kidney function. Total, high, medium, and low molecular weight ADPN and insulin levels were measured in 47 consecutive normoalbuminuric, normotensive T1D patients without retinopathy and in 47 age-, sex-, and body mass index-matched controls. Glomerular filtration rate was estimated by (51)Cr-EDTA plasma clearance. Total and high molecular weight ADPN ratio were higher in T1D patients than in controls. ADPN levels were not related to anthropometric measures, whereas they were in controls. In T1D, ADPN levels were not related to glycosylated hemoglobin, diabetes duration, or glomerular filtration rate. Peripheral insulin levels were higher in T1D patients than in controls, but they were not related to ADPN levels. In controls, insulin levels were positively related to total ADPN. In T1D without microangiopathy, high ADPN levels could not be related to anthropometric diabetes parameters, kidney function, or high insulin levels. The nature of this elevation remains unknown.
    The Journal of Clinical Endocrinology and Metabolism 03/2011; 96(3):E485-7. DOI:10.1210/jc.2010-1835 · 6.31 Impact Factor
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    ABSTRACT: As the impact of diabetes control was not tested on adiponectin (ADPN) levels, this study was designed to assess whether or not controlling hyperglycaemia can affect ADPN. A total of 15 T1D and 48 T2D patients with HbA(1c) greater than 10% were studied at the time of hospitalization for uncontrolled diabetes. Total, and high-, medium- and low-molecular-weight (HMW, MMW, LMW) ADPN were measured at the time of study inclusion, on days 1 and 8, and at 1, 3 and 6 months after insulin treatment. While diabetes control improved, total and HMW APDN decreased on days 1 and 8, but remained steady thereafter in T2D patients. In T1D patients, ADPN levels remained unchanged throughout the study. Glycaemic control with insulin reduces ADPN in T2D patients in the short-term, but was ineffective in T1D.
    Diabetes & Metabolism 02/2011; 37(3):259-61. DOI:10.1016/j.diabet.2010.12.001 · 2.85 Impact Factor
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    ABSTRACT: Adiponectin levels are high in cases of diabetic nephropathy, but it remains unclear whether these high levels are a cause or a consequence of the disease. We investigated the possible association of polymorphisms in the adiponectin gene and baseline adiponectin levels with the incidence of renal events in subjects with type 2 diabetes. We studied three adiponectin polymorphisms (-11391G > A, +45T > G and +276G > T) in 3086 subjects with type 2 diabetes and high levels of albumin excretion from the diabetes, hypertension, microalbuminuria or proteinuria, cardiovascular events and ramipril (DIABHYCAR) trial. Baseline concentrations of total adiponectin and of adiponectin isoforms were determined in cases with incident renal events and in controls matched for sex, age, body mass index (BMI) and adiponectin genotype. We used another cohort of type 2 diabetes patients-the survie, diabète de type 2 et génétique(SURDIAGENE) study (n = 1004)-for the replication of genetic data. In DIABHYCAR, the -11391A and +45G alleles were associated with a higher incidence of renal events [hazard ratio (HR) = 1.73; 95% confidence interval (CI), 1.10-2.71; and HR = 1.68; 95% CI, 1.14-2.47, respectively]. The haplotype containing susceptibility alleles, -11391A/+45G/+276G, was more frequent in cases with renal events (5.1% vs. 1.9% in those without, P = 0.005). In SURDIAGENE, the -11391A/+45G/+276G haplotype was also associated with renal events (5.6% vs. 1.9% in those without, P = 0.03). In DIABHYCAR, all isoforms were more abundant in subjects carrying the -11391A or +45G alleles. Medium- (MMW) and low-molecular weight (LMW) isoforms were more abundant in cases with renal events. In subjects with type 2 diabetes and early renal dysfunction, adiponectin gene variants are determinants of the renal risk. The -11391A and +45G alleles may affect renal risk by leading to high circulating adiponectin concentrations, at least those of MMW and LMW isoforms.
    Nephrology Dialysis Transplantation 07/2010; 25(7):2231-7. DOI:10.1093/ndt/gfp771 · 3.49 Impact Factor
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    ABSTRACT: The polymorphisms rs198358, rs5068 and rs632793 in the natriuretic peptide precursor A-B gene region [encoding atrial natriuretic peptides (ANP) and brain natriuretic peptides (BNP)] have been recently associated with ANP and BNP plasma concentrations and blood pressure (BP) in a large cohort study. We observed that GCG, the haplotype based on these polymorphisms and combining the three rare alleles associated with higher natriuretic peptides and lower BP in a recent report, was associated with BNP plasma levels and BP in a French study of 5212 middle-aged participants, Epidemiological Data on Insulin Resistance Syndrome study. With the 9-year follow-up of Epidemiological Data on Insulin Resistance Syndrome study, we were able to analyze the association of incident microalbuminuria (576 patients) and low estimated glomerular filtration rate (<60 ml/min; 246 incident patients) with the tested haplotypes. No haplotype, including GCG, the one combining the three rare alleles, was associated with incident patients of either microalbuminuria [odds ratio 1.27 (0.91-1.78), P = 0.15] or low estimated glomerular filtration rate [odds ratio 0.88 (0.54-1.46), P = 0.63]. This was consistent with a lack of effect on clinical renal outcomes found in previous studies and showed that even replicated and biologically plausible genetic association studies based on surrogate markers do not easily translate into clinically meaningful prognosis.
    Journal of Hypertension 06/2010; 28(6):1230-3. DOI:10.1097/HJH.0b013e328338a901 · 4.22 Impact Factor
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    ABSTRACT: Ethnic differences may affect the association of adiponectin (Ad) multimers with coronary artery disease (CAD). We analyzed the associations of total Ad, Ad multimers, and T45G polymorphism of ADIPOQ gene with pre-existing CAD. We carried out a cross-sectional study of 216 Afro-Caribbean type 2 diabetic (T2D) subjects. Levels of total Ad, high molecular weight (HMW), middle molecular weight (MMW), and low molecular weight (LMW) isoforms were measured. Subjects were genotyped. Of the subjects studied, 57 had pre-existing CAD, 77% of whom have had myocardial infarction. Subjects with CAD had lower Ad levels (total and multimers) and a higher frequency carried the minor allele 45G, GG/TG, (18% vs. 8%, P = 0.03) than subjects without CAD. In logistic regression analysis, the models used evaluate Ad in the context of adjustment for metabolic syndrome characteristics. The adjusted odds ratio (OR) of CAD was increased significantly (by factors of 1.05-3.27) for males, older subjects, low high-density lipoprotein cholesterol (HDL-C), high triglycerides (TGs), and carriers of the 45 G allele. For Ad, in model 1 (including only total Ad) the adjusted OR was 2.30; P = 0.03 and, in model 2 (including the three multimers, but not total Ad), the adjusted ORs were 0.73; P = 0.52 (HMW), 2.90; P = 0.01 (MMW), and 2.08; P = 0.09 (LMW). The T45G polymorphism in the ADIPOQ gene and hypoadiponectinemia were associated with CAD in our T2D subjects of predominantly African background. This effect of Ad level was mainly related to the MMW Ad form.
    Obesity 12/2009; 18(7):1466-8. DOI:10.1038/oby.2009.441 · 4.39 Impact Factor
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    ABSTRACT: Previous evidence has suggested that a low sex hormone-binding globulin (SHBG) concentration is associated with insulin-resistance and a low adiponectin concentration. We investigated the association between SHBG and the risk of hyperglycemia in each sex and we determined potential interactions between SHBG and adiponectin levels in the development of dysglycemia. We used a nested case-control design in the large prospective study, Data from an Epidemiological Study on the Insulin Resistance Syndrome (DESIR). We studied 227 men and women who were normoglycemic at baseline but hyperglycemic at 3 years (glycemia > or = 6.1 mmol/l or type 2 diabetes). They were matched for sex, age, and body mass index with 227 subjects who remained normoglycemic at 3 years. At baseline, the concentration of SHBG was significantly lower in women who subsequently developed hyperglycemia than in those who remained normoglycemic, with no difference for men. In multiple regression, SHBG at baseline was as an independent determinant of plasma adiponectin levels, in both women (P<0.0001) and men (P=0.002). In multivariate conditional logistic regression taking into account physical activity and changes in waist circumference over the follow-up, plasma SHBG remained significantly associated with the development of hyperglycemia in women but not in men. These associations persisted after adjustment for fasting insulinemia, high fasting glucose, and adiponectin levels. These findings suggest that a low SHBG level is a strong risk marker for dysglycemia in women, independently of both adiponectinemia and insulinemia. SHBG may therefore improve the identification of women at risk of diabetes.
    European Journal of Endocrinology 06/2009; 161(1):81-5. DOI:10.1530/EJE-09-0202 · 3.69 Impact Factor
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    ABSTRACT: Serum leptin has been reported to be associated in a sex-dependent manner with C-reactive protein (CRP), independently of adiposity. We tested the hypothesis that leptin is associated, independently of anthropometry indexes and in a sex-dependent way, with other inflammatory markers and variables related to metabolic syndrome (MS). In 384 healthy middle-aged adults (192 men and 192 women) total fat mass (FM), waist circumference (WC), serum leptin and 15 MS-related parameters (systolic and diastolic blood pressure, triglycerides, cholesterol, high density lipoprotein (HDL)-cholesterol, apo AI and B, fasting glucose, uric acid, CRP, orosomucoid and haptoglobin levels and aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT) and gamma-glutamyl transferase (GGT) activities) were measured. After adjustment for age, alcohol and cigarette consumption, WC, and total FM, leptin concentration was significantly associated with serum triglycerides, total cholesterol, apo B, uric acid and haptoglobin concentrations and liver enzyme activity only in men, and with apo AI, HDL-cholesterol (only borderline) and CRP only in women. Sex interaction terms were significant for total cholesterol, apo B, HDL cholesterol, uric acid, ALAT and GGT, and borderline significant for triglycerides, apo AI and ASAT. In this healthy population, leptin is significantly associated with various MS factors, independently of WC and total FM, depending on gender. Our study provides further evidence of sex-related differences mediated by leptin in inflammatory mechanisms and other MS-related metabolic pathways.
    Obesity 06/2009; 18(1):196-201. DOI:10.1038/oby.2009.156 · 4.39 Impact Factor
  • Diabetes & Metabolism 03/2009; 35. DOI:10.1016/S1262-3636(09)71914-7 · 2.85 Impact Factor
  • Diabetes & Metabolism 03/2009; 35. DOI:10.1016/S1262-3636(09)71805-1 · 2.85 Impact Factor
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    ABSTRACT: The role of NEFA in eating behaviour is still poorly known. Our objective was to examine whether etomoxir (ETO), an inhibitor of NEFA oxidation, or ( - )-hydroxycitrate (HCA), an inhibitor of lipogenesis which may indirectly stimulate NEFA oxidation, alters satiety. Post-lunch satiety was measured in eight normal-weight male subjects who were deprived of time cues and received on three occasions either ETO (320 mg), HCA (2 g) or placebo (PLA) in random order. Between lunch and dinner, blood was withdrawn continuously and collected every 10 min for measures of plasma concentrations of glucose, insulin, lactate, TAG, NEFA, beta-hydroxybutyrate (BHB), leptin and ghrelin. Results showed that HCA began to decrease hunger and desire to eat compared to PLA and ETO 210 min after lunch and increased satiety duration compared to PLA by 70 (se 23) min (P < 0.05), but did not modify energy intake at dinner. ETO did not affect any variable of satiety. HCA increased NEFA concentrations during the pre-dinner period, whereas ETO increased and decreased plasma concentrations of NEFA and BHB, respectively. Mean differences in plasma NEFA concentrations between HCA and PLA were predictive of the differences in satiety duration between treatments (r2 0.71, P < 0.01). Among treatments, plasma leptin concentration at dinner onset was the only blood variable correlated with energy intake at this meal (r - 0.75, P < 0.0005). In healthy, normal-weight men, acute HCA increased the intensity and duration of satiety possibly via increased NEFA disposal for oxidation.
    The British journal of nutrition 12/2008; 101(12):1867-77. DOI:10.1017/S0007114508143604 · 3.34 Impact Factor
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    ABSTRACT: Introduction Les taux d’adiponectine sont bas dans le diabète de type 2 et la maladie coronarienne mais élevés dans la maladie rénale. L’adiponectine circule sous 3 isoformes de poids moléculaire haut (HMW), moyen (MMW) et bas (LMW). Notre but est d’étudier la relation de l’adiponectine (totale et isoformes) et des polymorphismes (SNP) de son gène avec l’incidence d’infarctus du myocarde et/ou d’événements rénaux chez les diabétiques de type 2. Patients et méthodes Les génotypes pour 3 SNP (−11391G>A, +45T>G, +276G>T) ont pu être déterminés chez 3 086 diabétiques, micro- ou macroalbuminuriques (DIABHYCAR). L’adiponectine totale et ses isoformes ont été mesurées à l’entrée dans l’étude par ELISA chez les patients ayant subi un infarctus (n = 95) ou un événement rénal (n = 75) au cours du suivi et leurs témoins appariés pour le sexe, l’âge, l’IMC et le génotype. Résultats Les formes MMW et LMW sont plus élevées chez les patients avec événement rénal que chez leurs témoins : respectivement 1,66 vs 1,37 μg/ml, p = 0,02 et 2,20 vs 1,94 μg/ml, p = 0,05. Chez les sujets avec infarctus, on observe une tendance à une adiponectine HMW basale basse (2,16 vs 2,62 μg/mL, p = 0,07). Les porteurs de -11391A ont des concentrations plus élevées d’adiponectine totale (7,42 vs 5,72 μg/ml, p < 0,001), HMW (3,55 vs 2,33 μg/mL, p < 0,001) et MMW (1,69 vs 1,36 μg/ml, p = 0,03) que les homozygotes GG. Chez les homozygotes +45GG, toutes les concentrations sont plus élevées que chez les porteurs de +45T (totale : 8,89 vs 5,90 μg/ml, p = 0,01 ; HMW : 3,10 vs 2,48 μg/ml, p = 0,008 ; MMW : 2,05 vs 1,40 μg/ml, p = 0,01 ; LMW : 2,52 vs 1,95 μg/ml, p = 0,02). Discussion Chez les diabétiques de type 2 micro- ou macroalbuminuriques, une élévation des isoformes de poids moléculaire moyen et bas précède la survenue d’un événement rénal. De plus, les génotypes associés à des concentrations basales élevées sont également prédictifs de l’événement rénal. Conclusion Ces résultats sont en faveur d’un rôle causal d’une élévation d’adiponectine dans la néphropathie diabétique.
    Diabetes & Metabolism 03/2008; 34. DOI:10.1016/S1262-3636(08)72907-0 · 2.85 Impact Factor
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    ABSTRACT: Introduction L’ANP (peptide atrial natriurétique) a des propriétés hémodynamiques, mais aussi métaboliques, induisant la lipolyse chez l’Homme. Des études transversales ont montré que ses taux circulants étaient diminués chez l’obèse ou dans le syndrome métabolique, sans établir de lien de causalité. Nous avons étudié l’effet de variants de NPPA, le gène de l’ANP, sur les facteurs de risque cardiométaboliques dans DESIR (Données Épidémiologiques sur le Syndrome d’Insulino-Résistance), une cohorte issue de la population générale. Matériels et méthodes DESIR a inclus 2 576 hommes et 2 636 femmes, suivis 9 ans. Trois polymorphismes (rs5063, 664G>A ; rs5064, 708C>T et rs5065, 2238T>C), sélectionnés dans HapMap, ont été génotypés. L’association des caractéristiques à 0, 3, 6 et 9 ans des sujets avec les génotypes et les haplotypes de NPPA a été testée, chez tous les sujets et ceux en surpoids (Indice de Masse Corporelle, IMC>25 kg/m2). Le syndrome métabolique a été défini selon l’International Diabetes Federation. Résultats Le polymorphisme 708C>T était associé avec le HDL-cholesterol (1,63 ± 0,01 mM (n = 4190), 1,63 ± 0,01 (n = 867) et 1,77 ± 0,06 (n = 50) pour les génotypes 708CC, CT et TT respectivement, p = 0,04 ajusté pour le sexe, l’âge et l’IMC). Chez les sujets en surpoids, le tour de taille, les triglycérides et les ALAT étaient associés avec 708C>T et/ou 2238T>C, après ajustement sur le sexe et l’âge. Comparé à l’haplotype de référence, GCT (664G-708C-2238T, fréquence : 79 %), GTC (fréquence : 10 %) était associé à un tour de taille inférieur (94,2 cm, IC95 % [91,6-97,0] vs 96,8, IC95 % [94,8-99,0] p = 0,005), des triglycerides (1,24 mM, IC95 % [0,80-1,68] vs 1,50, IC95 % [1,42-1,56] p = 0,02) et des ALAT plus bas (24,0 UI/l, IC95 % [20,7-27,3] vs 27,5, IC95 % [25,2-29,8] p = 0,008) à l’inclusion, ainsi qu’à un risque réduit de développer un syndrome métabolique au cours du suivi (Rapport de Risque 0,77, IC 95 % [0,62-0,96], p = 0,02). Conclusion Les polymorphismes et haplotypes de NPPA sont associés dans l’étude DESIR, avec plusieurs composants du syndrome métabolique à l’entrée dans l’étude et avec l’incidence du syndrome métabolique au cours du suivi, chez les sujets en surpoids. Ceci pourrait être la conséquence des propriétés lipolytiques de l’ANP.
    Diabetes & Metabolism 03/2008; 34. DOI:10.1016/S1262-3636(08)72901-X · 2.85 Impact Factor
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    ABSTRACT: Introduction Les déterminants génétiques du DT2 sont mal identifiés et souvent les études initiales non répliquées. Au-delà de leurs propriétés hémodynamiques, les peptides natriurétiques, inducteurs de la lipolyse, ont un rôle métabolique. Un SNP du promoteur du gène du BNP rs198389, a été associé au DT2 dans une étude cas-contrôle. Nous avons étudié l’association de plusieurs SNPs du BNP et des haplotypes qu’ils définissent avec la glycémie, la prévalence et l’incidence du diabète au cours du suivi de DESIR. Matériels et méthodes DESIR a inclus 5 212 sujets, suivis 9 ans. Cinq SNPs (rs198389, rs198388, rs198381, rs12406383, rs12406089), sélectionnés dans Hap-Map, ont été génotypés. L’association des caractéristiques des sujets, dont la glycémie, de la prévalence du diabète à l’inclusion, et de l’incidence des nouveaux cas de diabète au cours des 9 ans de suivi, avec les génotypes et les haplotypes de NPPA a été testée (logiciel THESIAS). Résultats Cinq haplotypes décrivaient 99,9 % des haplotypes théoriques : TCACC, 55 %, est la référence haplotype, CTAAG, 28 %, CTGCC, 8 %, CTGCG, 6 % et TTACC, 2 %. La glycémie initiale était marginalement supérieure chez les porteurs de CTGCC et CTGCG (Tableau : effet haplotyhaplotypique estimé). 336 sujets étaient diabétiques à l’inclusion. 203 sont devenus diabétiques au cours du suivi. Les haplotypes CTGCC, CTGCG et TTACC étaient associés au risque de diabète (Tableau 1). L’analyse génotypique du SNP du promoteur, rs198389, retrouvait une association marginale avec l’incidence du diabète. Conclusion Certains haplotypes du gène du BNP sont associés au risque de diabète de type 2, en analyse transversale mais aussi longitudinale. Ces résultats étendent l’étude d’association antérieure et suggèrent un rôle causal du système des peptides natriurétiques dans la physiopathologie du diabète. Tableau 1. Odds RatioView Within Article
    Diabetes & Metabolism 03/2008; 34. DOI:10.1016/S1262-3636(08)72902-1 · 2.85 Impact Factor
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    ABSTRACT: Although cross-sectional studies have associated the Pro12Ala polymorphism of PPARG with type 2 diabetes, prospective studies offer more opportunities to investigate genetic variants. Associations between PPARG polymorphisms with insulin resistance parameters and with the 6-year incidence of impaired fasting glucose or type 2 diabetes were tested in 3,914 French Caucasians from the DESIR (Data From an Epidemiological Study on the Insulin Resistance Syndrome) cohort. In subjects normoglycemic at baseline (n = 3,498), the 6-year risk of hyperglycemia was lower in PPARG Ala carriers (odds ratio [OR] vs. ProPro = 0.66 [95% CI 0.44-0.99], P = 0.046 adjusted for sex, age, and BMI). Similar results were found with the PPARG C1431T single nucleotide polymorphism (SNP; adjusted OR = 0.65 [0.44-0.96], P = 0.036). Both alleles are in strong linkage disequilibrium (D' = 0.669, P < 0.001). The baseline mean fasting insulin and homeostasis model assessment of insulin resistance (HOMA-IR) were lower in Ala carriers compared with ProPro homozygotes (P = 0.001 for both), with smaller increases in mean insulin and HOMA-IR during follow-up (P = 0.007 and 0.018, respectively). No association with insulin levels or HOMA-IR was found with C1431T. In this cohort, the APM1 G-11391A SNP is associated with the development of hyperglycemia. The combined effects of PPARG Pro12Ala and APM1 G-11391A SNPs showed no interaction on the risk of 6-year hyperglycemia. The PPARG Ala allele showed a relatively high protective effect in developing hyperglycemia and hyperinsulinemia during a 6-year period. Cumulative rather than synergistic effects of PPARG Pro12Ala and APM1 SNPs on diabetes risk are suggested.
    Diabetes 04/2006; 55(4):1157-62. DOI:10.2337/diabetes.55.04.06.db05-0676 · 8.47 Impact Factor
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    ABSTRACT: To investigate in man the consequence on body composition and related biological and metabolic parameters of omitting or adding a meal. Twenty-four young normal-weight male subjects were recruited, 12 usual four-meal and 12 usual three-meal eaters, differing only in the consumption of an afternoon meal. They omitted or added a fourth meal during a 28-day habituation period and were asked to report their intake on three 3-day occasions. Before and after this habituation period, subjects participated in a session with a time-blinded procedure, and blood was collected continuously from lunch to the spontaneously requested dinner. Body composition, respiratory quotient, and biochemical parameters were measured in the late evening preceding each session. Omitting a meal was followed by increases in fat mass (360 +/- 115 grams, p < 0.05), late evening leptin concentration (20.7 +/- 11.0%, p < 0.05), and respiratory quotient (3.7 +/- 1.4%, p < 0.05). Increase in the percentage of dietary fat during the habituation period (+4.1 +/- 2.0%, p < 0.05) was correlated with fat mass (r = 0.66, p < 0.05). Adding a meal had no effect, but, in both groups, the change in energy content at this fourth eating occasion was correlated with the change in adiposity. Our results suggest that adiposity may increase when young lean male subjects switch from a four- to a three-meal pattern by removing their usual afternoon meal. This effect could be partly mediated by a change in the macronutrient composition of the diet.
    Obesity 03/2006; 14(2):215-27. DOI:10.1038/oby.2006.28 · 4.39 Impact Factor
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    ABSTRACT: The aim of this study was to find out whether high altitude (HA)-induced hypophagia was macronutrient-specific using a self-selection procedure. Body composition was assessed by dual X-ray absorptiometry before and after exposure and by dissection at the end of the experiment. Energy intake, macronutrient selection, body composition, plasma insulin and leptin concentrations were measured in rats (FHx) exposed 16 h daily for 10 days to hypobaric hypoxia (HH) simulating an altitude of 5500 m. Rats were fasted during the exposure to HH and had access to food only during the 8 h of normoxia in their active period. This group was compared to control group (C) with ad libitum access to food and a group of rats submitted only to the 16-h fast (FNx). Results showed that sustained hypophagia was specific to protein (55 +/- 5% of C, P < .05), whereas after a decline, carbohydrate intake reached its basal level on the 5th day. HH dramatically reduced fat-free mass gain (P < .05 and P < .0001 compared to C and FNx, respectively). Plasma leptin concentrations at the onset of the period of access to food were not significantly different from those of controls. Across groups, leptin was positively correlated with fat mass (r = .71, P < .001) and negatively with energy intake (r = -.52, P < .05), more specifically with protein intake (r = -.57, P < .05). These results suggest that HA leads to a reduced preference for protein impairing fat-free mass gain and that leptin may contribute to this hypophagia.
    Physiology & Behavior 10/2005; 86(1-2):145-53. DOI:10.1016/j.physbeh.2005.07.003 · 3.03 Impact Factor
  • B. Gatta, R. Aubert, D. Chapelot
    Annales d Endocrinologie 10/2005; 66(5):418-418. DOI:10.1016/S0003-4266(05)81873-6 · 0.66 Impact Factor

Publication Stats

1k Citations
160.42 Total Impact Points

Institutions

  • 2014
    • Paris Diderot University
      Lutetia Parisorum, Île-de-France, France
  • 2009–2011
    • University of Paris-Est
      La Haye-Descartes, Centre, France
  • 2010
    • Unité Inserm U1077
      Caen, Lower Normandy, France
  • 2006–2010
    • French Institute of Health and Medical Research
      • Unit of Genetic Determinants for Type 2 Diabetes and its Vascular Complications
      Lutetia Parisorum, Île-de-France, France