Publications (2)2.73 Total impact
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ABSTRACT: The subcellular distribution of glutamate decarboxylase (GAD) was revealed by electron microscopic immunocytochemistry in primary cultures of the rat neostriatum. Immunoreactivity was restricted to a small nerve cell type with rich amounts of free ribosomes and an indented nucleus. Glial cells were non-reactive. The results indicate that GABA-synthesizing neurons are viable in culture conditions and suggest that neostriatal GABA-neurons are small cells with simple ultrastructure since larger cells did not possess immunoreactivity.Brain Research 09/1981; 219(1):202-7. · 2.73 Impact Factor
Article: Demonstration of enkephalin-, substance P- and glutamate decarboxylase-like immunoreactivity in cultured cells derived from newborn rat neostriatum.[show abstract] [hide abstract]
ABSTRACT: The presence of cells exhibiting leucine-enkephalin-, substance P- and glutamate decarboxylase-like immunoreactivity was demonstrated in dissociated cultures from newborn rat neostriatum. The size and shape of the enkephalin-immunoreactive cells varied, but they were generally larger than substance P- and glutamate decarboxylase-immunoreactive cells, which formed relatively uniform cell populations. Cells of apparently non-neuronal origin did not show any immunoreactivity. It is unlikely that enkephalin is present in the same cells that contain substance P or glutamate decarboxylase because of morphological differences between these cells. The possible coexistence of substance P and glutamate decarboxylase in the same cells however, could not be excluded. The results of this study confirm that the cell bodies of neurons containing three possible neurotransmitters are located in the neostriatum.Histochemistry 02/1980; 69(2):169-79.