P Larsson

Publications (3)4.15 Total impact

• Article: Diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) for detection of class-specific antibodies to Aspergillus fumigatus and Candida albicans.

  S Lange, H Nygren, J E Brorson, I Holmberg, P Larsson
  Acta pathologica et microbiologica Scandinavica. Section C, Immunology 01/1982; 89(6):387-9.
• Article: Diffusion-in-gel enzyme linked immunosorbent assay (DIG-ELISA) for detection of antibodies to Yersinia enterocolitica O:3.

  S Lange, H Gunnarsson, P Larsson, H Nygren
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  ABSTRACT: The Diffusion-In-Gel Enzyme Linked Immunosorbent Assay (DIG-ELISA) is a new and simple method for the quantitation of antibodies to diffuse from wells in gel in petri dishes and absorb to an antigen coated to the plastic surface prior to testing. The antigen-antibody reaction is visualized with horseradish-peroxidase conjugated class-specific anti-immunoglobulins. The DIG-ELISA permits detection of 0.1 microns/ml of specific antibodies. This method was used to determine the IgG, IgA and IgM antibody levels to Yersinia enterocolitica O:3 in sera from patients with antibody titres to this microorganism, as determined by direct agglutination and complement-fixation test. The DIG-ELISA IgG antibody values, in contrast to IgA and IgM, correlated well to titres obtained by the direct agglutination method. Low degrees of correlations were found for all three immunoglobulin classes when compared to the complement fixation test.
  Acta pathologica et microbiologica Scandinavica. Section B, Microbiology 05/1981; 89(2):63-6.
• Article: Comparison of diffusion-in-gel enzyme-linked immunosorbent assay with conventional serological methods for detection of class-specific antibodies to Salmonella typhi O antigen.

  S Lange, H Elwing, P Larsson, H Nygren
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  ABSTRACT: The diffusion-in-gel enzyme-linked immunosorbent assay (DIG-ELISA) is a new and simple method for quantitation of antibodies, based on the ability of antibodies to diffuse from wells in gel and adsorb to antigen which is bound to a polystyrene surface. The antigen-antibody reaction is visualized with a color reaction caused by horseradish peroxidase-conjugated class-specific anti-immunoglobins. This method was used to study the immunoglobulin G, A, and M immune response to Salmonella typhi O antigen in individuals immunized with a monovalent heat-inactivated typhoid vaccine. The antibody values obtained by the DIG-ELISA method correlated with those evaluated by conventional direct agglutination (Widal) and indirect hemagglutination methods. The DIG-ELISA method was also found to be sensitive, specific, and economical, as well as suitable for handling large numbers of sera while requiring very simple equipment.
  Journal of Clinical Microbiology 12/1980; 12(5):637-40. · 4.15 Impact Factor