Peter Schumann

Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswyck, Lower Saxony, Germany

Are you Peter Schumann?

Claim your profile

Publications (379)813.89 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Three halophilic mycelium-forming actinobacteria, strains H195(T), H150 and H151, were isolated from a Saharan soil sample collected from Béni-isguen in the Mzab region (Ghardaïa, South of Algeria) and subjected to a polyphasic taxonomic characterisation. These strains were observed to show an aerial mycelium differentiated into coccoid spore chains and fragmented substrate mycelium. Comparative analysis of the 16S rRNA gene sequences revealed that the highest sequence similarities were to Saccharopolyspora qijiaojingensis YIM 91168(T) (92.02 % to H195(T)). Phylogenetic analyses showed that the strains H195(T), H150 and H151 represent a distinct phylogenetic lineage. The cell-wall hydrolysate was found to contain meso-diaminopimelic acid, and the diagnostic whole-cell sugars were identified as arabinose and galactose. The major cellular fatty acids were identified as iso-C15:0, iso-C16:0, iso-C17:0 and anteiso-C17:0. The diagnostic phospholipid detected was phosphatidylcholine and MK-9 (H4) was found to be the predominant menaquinone. The genomic DNA G+C content of strain H195(T) was 68.2 mol%. On the basis of its phenotypic features and phylogenetic position, we propose that strain H195(T) represents a novel genus and species, Mzabimyces algeriensis gen. nov., sp. nov., within a new family, Mzabimycetaceae fam. nov. The type strain of M. algeriensis is strain H195(T) (=DSM 46680(T) = MTCC 12101(T)).
    Antonie van Leeuwenhoek 09/2014; · 2.07 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel Gram-staining-negative, aerobic, non-endospore-forming, non-pigmented, rod shaped, slightly moderately halophilic bacterium, designated GBPy5T was isolated from aquatic plants in Gomishan wetland, Iran. Cells of strain GBPy5T were motile. Growth occurred between 1-10 % (w/v) NaCl and the isolate grew optimally at 3 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 8.0 and 30 oC, respectively, while it was able to grow over a pH range of 6.5-9.0 and a temperature range of 4-35 oC. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GBPy5T is a member of the genus Pseudomonas forming a monophyletic branch. The novel strain exhibited a 16S rRNA gene sequence similarity of 95.4 % with the type strains of Pseudomonas guariconensis PCAVU11T and P. sabulinigri J64T, respectively. The major cellular fatty acids of the isolate were C18:1 ω7c (37.8 %), C16:0 (14.9 %), C16:1 ω7c (12.9 %), C12:0 3OH (7.1 %) and C12:0 (7.0 %). The polar lipids pattern of strain GBPy5T consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The G+C content of the genomic DNA of this strain was 59.2 mol%. On the basis of the phenotypic and phylogenetic data, strain GBPY5T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salegens sp. nov. is proposed. The type strain is strain GBPy5T (= IBRC-M 10762T= CECT 8338T).
    International journal of systematic and evolutionary microbiology. 07/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel Gram-staining-positive, moderately halophilic bacterium, designated strain B6BT, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain B6BT were rod-shaped, motile and producing ellipsoidal endospores at a terminal position in non-swollen sporangia. Strain B6BT was a strictly aerobic bacterium and catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5-20.0 % (w/v), with optimum growth occurring at 10.0 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain B6BT was shown to belong to the phylum Firmicutes and the closest phylogenetic similarities were with the species Virgibacillus koreensis BH30097T (97.5 %), Virgibacillus albus YIM 93624T (97.4 %), Sediminibacillus halophilus EN8dT (96.8 %), Sediminibacillus albus NHBX5T (96.6 %), Virgibacillus carmonensis LMG 20964T (96.3 %) and Paraliobacillus quinghaiensis YIM-Ci58T (96.0 %), respectively. Phylogenetic analysis revealed that strain B6BT along with Virgibacillus koreensis BH30097Tand Virgibacillus albus YIM 93624Tclustered in a separate clad in the family Bacillaceae. The DNA G+C content of this new isolate was 35.8 mol %. DNA-DNA hybridization experiments revealed low levels of relatedness between strain B6BTand Virgibacillus koreensis BH30097T (13 %) and Virgibacillus albus (33 %).The major cellular fatty acid of strain B6BT was anteiso-C15:0 (75.1 %) and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipids and an unknown glicolipid. The isoprenoid quinones were MK-7 (90 %) and MK-6 (3 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features support the placement of isolate B6BT within Firmicutes, closely related to Virgibacillus koreensis and Virgibacillus albus but with features that clearly distinct it from those of the genus Virgibacillus or other related genera. On the basis of polyphasic evidence from this study, we propose the placement of strain B6BTwithin a new genus, as Aquibacillus halophilus gen. nov., sp. nov., with B6BT as type strain (=IBRC-M 10775T= KCTC 13828T) and the transfer of Virgibacillus koreensis and Virgibacillus albus to this new genus, as Aquibacillus koreensis comb. nov. and Aquibacillus albus comb. nov., respectively. The type strain of Aquibacillus koreensis is BH30097T (=KCTC 3823T=IBRC-M10657T=JCM 12387T) and the type strain of Aquibacillus albus is YIM 93624T (= DSM23711T= IBRC-M10798T= JCM 17364T).
    International journal of systematic and evolutionary microbiology. 07/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel Gram-staining-negative, motile, non-pigmented, facultative anaerobe, spirillum-shaped, halophilic and alkaliphilic bacterium, designated strain GCWy1T, was isolated from water of the coastal-marine wetland Gomishan in Iran. The strain was able to grow at NaCl concentrations of 1-10 % (w/v) and optimal growth was achieved at 3 % (w/v). The optimum pH and temperature for growth were pH 8.5 and 30 °C, while it was able to grow at pH 7.5-10 and 4-40 °C. Phylogenetic analysis based on the comparison of the 16S rRNA gene sequence placed the new isolate within the Gammaproteobacteria as a separate deep branch, with 92.1 % or lower sequence similarity to representatives of the genera Saccharospirillum and Reinekea and less than 91.0 % sequence similarity with other remotely related genera. The major cellular fatty acids of the isolate were C18:1ω7c, C16:0 and C17:0, and its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cells of strain GCWy1T contained the isoprenoid quinones Q-9 and Q-8 (81 %:2 %). The G+C content of the genomic DNA of this strain was 52.3 mol %. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and phenotypic data, strain GCWy1T represents a novel species in a new genus in the family Saccharospirillaceae, order Oceanospirillales, for which the name Salinispirillum marinum gen. nov., sp. nov. is proposed. The type strain of the type species is GCWy1T (= IBRC-M 10765T = CECT 8342T).
    International journal of systematic and evolutionary microbiology. 07/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Nocardia species are ubiquitous in the environment with an increasing number of species isolated from clinical sources. From 2005 to 2009, eight isolates (W9042, W9247, W9290, W9319, W9846, W9851(T), W9865, and W9908) were obtained from eight patients from three states in the United States and Canada; all were from males ranging in age from 47 to 81 years old; and all were obtained from finger (n = 5) or leg (n = 3) wounds. Isolates were characterized by polyphasic analysis using molecular, phenotypic, morphologic and chemotaxonomic methods. Sequence analysis of 16S rRNA gene sequences showed the eight isolates are 100 % identical to each other and belong in the genus Nocardia. The nearest phylogenetically related neighbours were found to be the type strains for Nocardia altamirensis (99.33 % sequence similarity), Nocardia brasiliensis (99.37 %), Nocardia iowensis (98.95 %) and Nocardia tenerifensis (98.44 %). The G+C content of isolate W9851(T) was determined to be 68.4 mol %. The DNA-DNA relatedness between strain W9851(T) and the N. brasiliensis type strain was 72.8 % and 65.8 % when measured in the laboratory and in silico from genome sequences, respectively, and 95.6 % ANI. Whole-cell peptidoglycan was found to contain meso-diaminopimelic acid; MK-8-(H4)ω-cyc was identified as the major menaquinone; the major fatty acids were identified as C16:0, 10 Me C18:0, and C18:1 w9c, the predominant phospholipids were found to include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; whole-cell sugars detected were arabinose and galactose; and mycolic acids ranging from 38 to 60 carbon atoms were found to be present. These chemotaxonomic analyses are consistent with assignment of the isolates to the genus Nocardia. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra of the clinical isolates showed genus and species level profiles that were different from other Nocardia species. All isolates were resistant to ciprofloxacin, clarithromycin and imipenem but were susceptible to amikacin, amoxicillin/clavulanate, linezolid and trimethoprim/sulfamethoxazole. The results of our polyphasic analysis suggest the new isolates obtained from wound infections represent a novel species within the genus Nocardia, for which the name Nocardia vulneris sp. nov. is proposed, with strain W9851(T) (= DSM 45737(T) = CCUG 62683(T) = NBRC 108936(T)) as the type strain.
    Antonie van Leeuwenhoek 07/2014; · 2.07 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel Promicromonospora strain, designated HM 792T, was isolated from soil in Fars Province, Iran. On ISP 2 medium, the yellow-pigmented isolate produced long and branched hyphae that developed into a large number of irregular shaped spores. It showed optimal growth at 25-30 °C and pH 6.0-9.0 with 0-8 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Promicromonospora. Whole cell hydrolyzates of strain HM 792T contained amino acids D-glutamic acid, L-alanine and L-lysine along with sugars glucose and ribose. The main polar lipids were diphosphatidylglycerol, two unknown phospholipids, two unknown glycolipids and two unknown glycophospholipids, complemented by minor concentrations of phosphatidylinositol and phosphatidylglyserol. MK-9(H4) was the predominant menaquinone. The fatty-acid pattern was mainly composed of the saturated branched-chain acids anteiso-C15:0 and iso-C15:0. The 16S rRNA gene sequence analysis showed the highest pairwise sequence identity (96.6-99.0 %) with the members of the genus Promicromonospora. Based on phenotypic and genotypic features, strain HM 792T (DSM 45554T = UTMC00792T = CCUG 63022T) was considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora iranensis is proposed.
    International journal of systematic and evolutionary microbiology. 07/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-reaction-positive bacterial isolate, designated Tü 6233T, with rudimentary and coral pink vegetative mycelium that did neither form aerial mycelia nor spores was isolated from a Brazilian soil sample. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. Cell wall hydrolysates contained meso-diaminopimelic acid as diagnostic diamino acid and galactose as diagnostic sugar. Major fatty acids were iso-C16:0, iso-C15:0 and C17:1ω8c and the predominant menaquinone was MK-9(H4). Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, an unknown glycophospholipid and an unknown phospholipid. The DNA G+C content of the strain was 75.4 mol %. The 16S rRNA gene sequence identity with members of the genus Geodermatophilus was 94.2-98.7 %. Based on phenotypic, chemotaxonomic and phylogenetic data strain Tü 6233T is proposed to represent a novel species, Geodermatophilus brasiliensis, with the type strain Tü 6233T (DSM 44526T = CECT 8402T).
    International journal of systematic and evolutionary microbiology 05/2014; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A halophilic actinomycete strain, designated H27(T), was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10-15 % (w/v) and the optimum growth temperature and pH were found to be 28-37 °C and 6-7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27(T) were identified as MK-11 (H4) and MK-10 (H6). The major fatty acids were found to be iso-C16:0, anteiso-C17:0, 10 methyl C17:0 and 10 methyl C16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27(T) are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27(T) is most closely related to Streptomonospora alba DSM 44588(T) (98.8 %) and Streptomonospora flavalba DSM 45155(T) (98.7 %) whereas the DNA-DNA relatedness values between strain H27(T) and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27(T) should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27(T) (=DSM 45604(T) =CCUG 63369(T) =MTCC 11563(T)).
    Antonie van Leeuwenhoek 05/2014; · 2.07 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A novel non-motile, Gram-staining-negative, yellow-pigmented bacterium, designated CT348(T), isolated from the ectorhizosphere of an organic olive tree in Spain and characterised as an efficient plant growth promoting bacterium, was investigated to determine its taxonomic status. The isolate grew best in a temperature range of 5-35°C, at pH 5.0-8.0 and with 0-1% (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Chryseobacterium. The DNA G+C content of the novel strain was 38.2mol%. The strain contained a polyamine pattern with sym-homospermidine as the major compound and produced flexirubin-type pigments. MK-6 was the dominant menaquinone and the major cellular fatty acids were iso-C15:0, C17:1ω9c, iso-C17:0 3-OH and iso-C15:0 2-OH. The main polar lipids were phosphatidylethanolamine and several unidentified lipids and aminolipids. The 16S rRNA gene showed 92.2-97.8% sequence identity with the members of the genus Chyseobacterium. Based on the phenotypic traits and DNA-DNA hybridizations with the type strains of the most closely related species, the isolate is shown to represent a novel species, Chyseobacterium oleae, type strain CT348(T) (=DSM 25575 =CCUG 63020). Emended descriptions of the genus Chryseobacterium and C. daecheongense, C. gambrini, C. gleum, C. joostei, C. jejuense, C. luteum, C. shigense, C. taiwanense, C. ureilyticum and C. vrystaatense are also proposed.
    Systematic and Applied Microbiology 05/2014; · 3.29 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel Gram-staining-positive, moderately halophilic bacterium, designated strain A76T, was isolated from the brine sample of the hypersaline lake Aran-Bidgol in Iran. Cells were strictly aerobic, coccus-shaped, non-motile, non-sporulating, catalase and oxidase positive. Strain A76T grew between pH 7.0-10.0 (optimal growth at pH 8.0), between 20-45°C (optimal growth at 35°C) and at salinities of 0.5 to 12.5% (w/v) NaCl (optimal growth at 7.5% [w/v] NaCl). On the basis of 16S rRNA gene sequence analysis, strain A76T was shown to belong to the phylum Firmicutes with sequence similarities of 94.1%, 93.1% and 91.1%, to the type species of the genera Jeotgalicoccus, Salinicoccus and Nosocomiicoccus, respectively. The DNA G+C content of this new isolate was 38.8 mol%. The major cellular fatty acids of strain A76T were anteiso-C15:0 and iso-C15:0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, an unknown lipid and two unknown phospholipids. The isoprenoid quinones were MK-6 (94%), MK-5 (3%) and MK-7 (3%). The amino acid constituents of the cell wall were Lys, Asp, Gly, Glu and Ala. The physiological, biochemical and phylogenetic differences between strain A76T and type strains of validly named taxa suggest that this strain represents a new species in a novel genus within the Staphylococcaceae, for which the name Aliicoccus persicus gen. nov., sp. nov. is proposed. The type strain of Aliicoccus persicus is strain A76T (=CECT 8508T =DSM 28306T =IBRC-M 10081T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2014; · 2.11 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: ABSTRACT A novel myxobacterium MCy1366T (Ar1733) was isolated in 1981 from a soil sample collected from a region near Tokyo, Japan. It displayed general myxobacterial features like Gram negative staining, rod shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting body-like aggregates and myxospore-like structures. The strain was mesophilic, aerobic and showed chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like cephalosporin C, kanamycin, gentamycin, hygromycin B, polymyxin and bacitracin and the key fatty acids of whole cell hydrolysates were iso-C15:0, iso-C17:0, and iso-C17:0 2-OH. The genomic G+C content of the novel strain is 65.6 mol%. The 16S rRNA gene sequence showed highest similarity (97.60%) to "Stigmatella koreensis" strain KYC-1019 (not validly described taxon, GenBank accession no. EF112185). The phylogenetic analysis based on 16S rRNA gene sequences and MALDI-TOF data revealed a novel branch in the family Myxococcaceae. DNA-DNA hybridization showed only 28% similarity between the novel strain and the closest species, Corallococcus exiguus DSM 14696T (97% 16S rRNA gene sequence similarity). A recent isolate from a Switzerland soil sample and designated reference strain, MCy10622 displayed 99.9% 16S rRNA gene similarity and showed almost the same characteristics with MCy1366T. Since some morphological features like fruiting body like aggregates were barely reproducible in the type strain, the newly isolated strain MCy10622 was also intensively studied. On the basis of a comprehensive taxonomic study, we propose a novel genus and species, Aggregicoccus edonensis gen. nov., sp. nov., for strain MCy1366T and MCy10622. The type strain for the genus Aggregicoccus is MCy1366T (=DSM 27872T =NCCB 100468T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2014; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new bacterium, PB3-7BT, was isolated on phenol-supplemented inorganic growth medium from a laboratory-scale wastewater purification system that treated coke plant effluent. The 16S rRNA gene sequence analysis revealed that strain PB3-7BT belonged to the family Alcaligenaceae and possessed the highest pairwise similarity values to Parapusillimonas granuli LMG 24012T (97.5%), Candidimonas bauzanensis DSM 22805T (97.3%) and Pusillimonas noertemannii DSM 10065T (97.2%). Strain PB3-7BT was rod-shaped, motile, oxidase and catalase positive. The predominant fatty acids were C16:0 (38.8%), cycloC17:0 (20.7%), cycloC19:0ω8c (16.9%), and C14:0 3-OH (10.3%), and the major respiratory quinone was Q-8. The G + C content of the genomic DNA of strain PB3-7BT was 59.7 mol%. The new bacterium can be distinguished from the closely related type strains based on its positive urease activity and capability for the assimilation of glycerol and amygdalin. On the basis of the phenotypic, chemotaxonomic and molecular data, strain PB3-7BT is considered to represent a new genus and species, for which the name Eoetvoesia caeni gen. nov., sp. nov. is proposed. The type strain is PB3-7BT (=DSM 25520T=NCAIM B 02512T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A facultative anaerobic, Gram-reaction-positive, catalase- and oxidase-negative, rod-shaped bacterium isolated from a human infected bite wound caused by a dog was characterized by phenotypic and molecular genetic methods. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMMIB Q2029717T was a member of the order Micrococcales of the class Actinobacteria, displaying 91.6% to 96% sequence similarities with members of the family Microbacteriaceae. Phylogentic trees generated by different algorithms indicated that the strain forms an independent phylogenetic line of descent that consistently clustered proximal to the base of the genus Leucobacter. Chemical studies revealed the presence of a cell-wall murein based on L-lysine (type B1α), major menaquinone (MK-10) and DNA G+C content of 56.9 mol%. The distinct phylogenetic position, ribotyping and MALDI-TOF profiles and the significant phenotypic differences clearly separate strain IMMIB Q2029717T from its nearest phylogenetic neighbour and support its classification as a new genus and species, Canibacter oris. The type strain is IMMIB Q2029717T (=DSM 27064T= CCUG 64069T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2014; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The Gram-stain-positive, endospore-forming, rod shaped, strictly aerobic, moderately halophilic bacterium, designated strain H9B, was isolated from the mud sample of hypersaline lake Aran-Bidgol in Iran. Cells of strain H9B were motile and produced colonies with a yellowish gray pigment. Growth occurred between 2.5-10 % (w/v) NaCl and the isolate grew optimally at 7.5 % (v/w) NaCl. The optimum pH and temperature for growth of the strain were pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of 6-10 and 25-45 °C, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H9B is a member of the genus Oceanobacillus. The closest relative to this strain was Oceanobacillus profundus CL-MP28T with 97.1 % 16S rRNA gene sequences similarity. Level of DNA-DNA relatedness between the novel isolate and this phylogenetically related species was 17%. The major cellular fatty acids of the isolate were anteiso-C15:0, anteiso-C17:0, iso-C15:0 and iso-C16:0. The polar lipid pattern of strain H9B consisted of phosphatidylglycerol, diphosphatidylglycerol, four phospholipids and an aminolipid. It contained MK-7 as the predominant menaquinone and meso-diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 37.1 mol%. Phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data suggest that this strain represents a novel species of the genus Oceanobacillus, for which the name Oceanobacillus limi sp. nov. is proposed. The type strain of Oceanobacillus limi is strain H9BT (= IBRC-M 10780 T= KCTC 13823T= CECT 7997).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-staining-positive actinobacterial strain Chem10T was isolated form soil around Inche-Broun hypersaline wetland in the north of Iran. Strain Chem10T was strictly aerobic, catalase and oxidase positive. The isolate grew between 0-3% NaCl, at 20-40 °C and at pH 6.0-8.0. The optimum temperature and pH for growth were 28-32 °C and pH 7.0, respectively. The cell wall of strain Chem10T contained meso-diaminopimelic acid as diaminoacid and galactose, ribose and arabinose as whole-cell sugars. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. It synthesized cellular fatty acids of the straight-chain saturated, monosaturated, iso and anteiso-branched types C14:0, C16:0, iso-C16:1, anteiso-C17:0, iso-C16:0, iso-C14:0 and iso-C15:0, and the major respiratory quinone was MK-9(H4). The G+C content of the genomic DNA was 70.7 mol %. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Chem10T belongs to the family Pseudonocardiaceae and showed the closest phylogenetic similarity with Alloactinosynnema album KCTC 19294T (98.3%) and Actinokineospora cibodasensis DSM 45658T (97.9%). DNA-DNA relatedness between the novel strain and strains A. album KCTC 19294T and A. cibodasensis DSM 45658T were only 52 % and 23% respectively. On the basis of phylogenetic analysis, phenotypic characteristics and DNA-DNA hybridization data, a novel species of the genus Alloactinosynnema is proposed, Alloactinosynnema iranicum sp. nov. The type strain is Chem10T (= IBRC-M 10403T = CECT 8209T). Besides, the emended description of the genus Alloactinosynema is proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2014; · 2.11 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Four isolates, designated, RG-1T, RG-3T, RG-6 and RG-8 with optimum growth temperatures around 60 °C were recovered from a hot stream near Ribeira Grande on the Island of São Miguel in the Azores. These strains were strictly aerobic, catalase and oxidase positive. The organisms could be divided into two groups; one comprising strains RG-1T and RG-6, the other formed by strains RG-3T and RG-8, with distinctive phenotypic and chemotaxonomic characteristics. Major fatty acids varied between the groups, but all contained iso-branched components such as iso-C16:0 10-methyl and iso-C17:0 10-methyl. Analysis of the 16S rRNA gene shows the strains to cluster with species of the genus Rubrobacter, specifically with Rubrobacter xylanophilus to which they have pairwise sequence similarity in the range of 98.4–98.6%. The DNA–DNA hybridization result show that strains RG-1T and RG-3T share 72% reassociation. Strains RG-1T and RG-3T displayed extreme tolerance to desiccation when compared to other species of the genus. Based on genotypic, physiological and biochemical characteristics we describe two new species: Rubrobacter calidifluminis sp. nov., represented by strain RG-1T (=CECT 8308T =JCM 19154T) and Rubrobacter naiadicus sp. nov. represented by strain RG-3T (=CECT 8310T = JCM 19155T). The description of the species Rubrobacter bracarensis [16] is emended to correct the fatty acid composition of these organisms. The discovery of new fatty acids in these species requires emendation of the description of the genus Rubrobacter.
    Systematic and Applied Microbiology 01/2014; · 3.29 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing-and UV-radiation, designated G18 T , was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15–35 ∘ C, at pH 5.5–9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H 4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C 16:0 and iso-C 15:0 and the unsaturated C 17:1 íµí¼”8c and C 16:1 íµí¼”7c. The 16S rRNA gene showed 97.4–99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18 T is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18 T (= DSM 44209 T = CCUG 63018 T). The INSDC accession number is HF970583. The novel R software package lethal was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.
    BioMed Research International 01/2014; · 2.88 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Earlier phylogenetic analyses of the marine Rhodobacteraceae (class Alphaproteobacteria) genera Leisingera and Phaeobacter indicated that neither genus might be monophyletic. We here used phylogenetic reconstruction from genome-scale data, MALDI-TOF mass-spectrometry analysis and a re-assessment of the phenotypic data from the literature to settle this matter, aiming at a reclassification of the two genera. Neither Phaeobacter nor Leisingera formed a clade in any of the phylogenetic analyses conducted. Rather, smaller monophyletic assemblages emerged, which were phenotypically more homogeneous, too. We thus propose the reclassification of Leisingera nanhaiensis as the type species of a new genus as Sedimentitalea nanhaiensis gen. nov., comb. nov., the reclassification of Phaeobacter arcticus and Phaeobacter leonis as Pseudophaeobacter arcticus gen. nov., comb. nov. and Pseudophaeobacter leonis comb. nov., and the reclassification of Phaeobacter aquaemixtae, Phaeobacter caeruleus, and Phaeobacter daeponensis as Leisingera aquaemixtae comb. nov., Leisingera caerulea comb. nov., and Leisingera daeponensis comb. nov. The genera Phaeobacter and Leisingera are accordingly emended.
    Frontiers in microbiology. 01/2014; 5:416.
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel Gram-negative, slightly halophilic bacterium, designated strain GASx41T, was isolated from soil of the coastal-marine wetland Gomishan in Iran. Cells of strain GASx41T were curved rods, ring-like or horseshoe-shaped and non-motile. Strain GASx41T was strictly aerobic, catalase and oxidase positive. The strain was able to grow at NaCl concentrations of 1-10 % (w/v), with optimum growth occurring at 2.5-3 % (w/v) NaCl. The optimum temperature and pH for growth were 25-30 ° and pH 7.5-8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain GASx41 was shown to belong to the genus Cyclobacterium within the phylum Bacteroidetes and showed closest phylogenetic similarity with Cyclobacterium jeungdonense HMD3055T (98.0 %). The DNA G+C content of strain GASx41T was 48.1 mol%. The major cellular fatty acids of strain GASx41T were iso-C15:0, summed feature 4 (iso-C15: 0 2-OH and/or C16:1 ω7c), anteiso-C15:0 2-OH, anteiso-C15:0 and iso- C17:0 3-OH and its polar lipid pattern consisted of phosphatidylethanolamine, phosphatidylcholine and twelve unknown lipids. The only quinone present was menaquinone 7 (MK-7). All these features confirmed the placement of isolate GASx41T within the genus Cyclobacterium. On the basis of evidence from this study, a novel species of the genus Cyclobacterium, Cyclobacterium halophilum sp. nov. is proposed, with strain GASx41T (= IBRC-M 10761T= CECT 8341T) as the type strain.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2013; · 2.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A novel halophilic actinomycete, strain designated H53(T), was isolated from a Saharan soil sample collected from Chaâbet Ntissa, Béni-isguen, Ghardaïa (South of Algeria) and was characterized taxonomically by means of polyphasic approach. Optimal growth was found to occur at 30-35 °C, pH 6-7 and in the presence of 15-25% (w/v) NaCl. The strain was observed to produce abundant aerial mycelium, which formed long chains of rod-shaped spores at maturity, and well developed and fragmented substrate mycelium. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were arabinose and galactose. The predominant menaquinones were found to be MK-9(H4) and MK-9(H6). The predominant cellular fatty acids were determined to be iso- and anteiso-C17:0, iso-C15:0, and cis9 iso-C17:1. The diagnostic phospholipid detected was phosphatidylcholine. The morphological and chemotaxonomic characteristics of the strain were consistent with those of members of the genus Saccharopolyspora. Phylogenetic analyses on the basis of the 16S ribosomal RNA (rRNA) gene sequence showed that this strain formed a distinct phyletic line within the radiation of the genus Saccharopolyspora. The 16S rRNA sequence similarities between strain H53(T) and other members of the genus Saccharopolyspora ranged from 92.1 to 94.3%. The DNA G+C content of strain H53(T) was 72.6%. The genotypic and phenotypic data showed that the strain H53(T) represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora ghardaiensis sp. nov. is proposed, with the type strain H53(T) (=DSM 45606(T)=CCUG 63370(T)=CECT 8304(T)).The Journal of Antibiotics advance online publication, 18 December 2013; doi:10.1038/ja.2013.136.
    The Journal of Antibiotics 12/2013; · 2.19 Impact Factor

Publication Stats

5k Citations
813.89 Total Impact Points

Institutions

  • 1993–2014
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2012–2013
    • École Normale Supérieure de Kouba
      Mastaganeam, Mostaganem, Algeria
    • Macquarie University
      Sydney, New South Wales, Australia
    • Carl von Ossietzky Universität Oldenburg
      • Department of Chemistry and Biology of the Marine Environment (ICBM)
      Oldenburg, Lower Saxony, Germany
  • 2009–2013
    • University of Tehran
      • • Department of Microbiology (VM)
      • • School of Biology
      Tehrān, Ostan-e Tehran, Iran
    • University of Delhi
      • Department of Zoology (Faculty of Science)
      Delhi, NCT, India
    • Mugla Üniversitesi
      Mobolla, Muğla, Turkey
    • National Academy of Agricultural Science (South Korea)
      Sŏul, Seoul, South Korea
    • National Institute for Biotechnology and Genetic Engineering
      Shah Faisalabad, Punjab, Pakistan
    • University of Haifa
      • Department of Evolutionary and Environmental Biology
      H̱efa, Haifa District, Israel
    • University of Innsbruck
      • Institut für Mikrobiologie
      Innsbruck, Tyrol, Austria
  • 2003–2013
    • Eötvös Loránd University
      • • Department of Microbiology
      • • Department of Genetics
      Budapest, Budapest fovaros, Hungary
  • 2009–2012
    • Justus-Liebig-Universität Gießen
      • Institut für Angewandte Mikrobiologie
      Gießen, Hesse, Germany
  • 2002–2012
    • University of Coimbra
      • • Departamento de Ciências da Vida
      • • Centro de Neurociências e Biologia Celular (CNC)
      Coimbra, Distrito de Coimbra, Portugal
    • Pacific Institute of Bioorganic Chemistry
      Wladiwostok, Primorskiy, Russia
  • 2011
    • Institute for Agricultural and Fisheries Research
      Meirelbeke, Flanders, Belgium
  • 2010–2011
    • University of Bonn
      • Institute of Medical Microbiology, Immunology and Parasitology
      Bonn, North Rhine-Westphalia, Germany
    • Centers for Disease Control and Prevention
      Atlanta, Michigan, United States
  • 2007–2011
    • Universidade Católica Portuguesa
      • Escola Superior de Biotecnologia
      Lisbon, Lisbon, Portugal
  • 2004–2011
    • Ghent University
      • Laboratory of Microbiology
      Gand, Flanders, Belgium
    • University of Reading
      • Food and Nutritional Sciences
      Reading, England, United Kingdom
    • French National Centre for Scientific Research
      Lutetia Parisorum, Île-de-France, France
    • Alexandria University
      • Department of Microbiology (Faculty of Veterinary Medicine)
      Alexandria, Alexandria, Egypt
  • 2001–2011
    • Humboldt-Universität zu Berlin
      • Department of Biology
      Berlin, Land Berlin, Germany
    • Leibniz Centre for Agricultural Landscape Research
      Muencheberg, Brandenburg, Germany
    • University of Milan
      • Department of Food Science and Microbiology DISTAM
      Milano, Lombardy, Italy
    • La Trobe University
      Melbourne, Victoria, Australia
  • 2006–2009
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • Biological Resource Center
      Anzan, Gyeonggi Province, South Korea
    • Centro de Investigaciones Biológicas del Noroeste
      La Paz, Baja California Sur, Mexico
  • 2003–2009
    • Yunnan University
      • • The Key Laboratory for Microbial Resources of the Ministry of Education
      • • Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources
      Yün-nan, Yunnan, China
  • 2008
    • Marquette University
      Milwaukee, Wisconsin, United States
  • 2006–2008
    • Tampere University of Technology
      • Department of Chemistry and Bioengineering
      Tampere, Western Finland, Finland
  • 2004–2008
    • Universidad de Salamanca
      • Departamento de Microbiología y Genética
      Salamanca, Castile and Leon, Spain
  • 2002–2008
    • University of Veterinary Medicine in Vienna
      • Institute of Bacteriology, Mycology and Hygiene
      Wien, Vienna, Austria
  • 2000–2008
    • Università degli Studi di Messina
      • Dipartimento di Scienze Biologiche e Ambientali
      Messina, Sicily, Italy
    • Spanish National Research Council
      • Institute for Natural Resources and Agrobiology of Sevilla
      Madrid, Madrid, Spain
  • 1999–2008
    • Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
      Jena, Thuringia, Germany
  • 2006–2007
    • Indian Agricultural Research Institute
      • Division of Nematology
      New Delhi, NCT, India
  • 2005–2007
    • University of Manitoba
      • Department of Microbiology
      Winnipeg, Manitoba, Canada
  • 2003–2007
    • Université de Bretagne Occidentale
      Brest, Brittany, France
  • 2002–2005
    • Russian Academy of Sciences
      • Institute of Microbiology
      Moscow, Moscow, Russia
    • Bundesanstalt für Geowissenschaften und Rohstoffe
      Hanover, Lower Saxony, Germany
  • 2002–2003
    • University of Vienna
      Wien, Vienna, Austria
  • 1999–2000
    • Christian-Albrechts-Universität zu Kiel
      • Institute for General Microbiology
      Kiel, Schleswig-Holstein, Germany