Peter Schumann

Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswyck, Lower Saxony, Germany

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Publications (388)827.39 Total impact

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    ABSTRACT: A recent study on members of the genus Actinobaculum revealed that cultures of the species Actinobaculum massiliense CCUG 47753T = DSM 19118T currently being distributed do not conform to the properties of the type strain of Actinobaculum massiliense CIP 107404T given by Greub & Raoult (2002). The original strain CIP 107404T is no longer available from the Biological Resource Center of Institut Pasteur, Paris. Based on data currently available the organism currently deposited as CCUG 47753T and DSM 19118T are members of the species Actinobaculum schaalii. Clearly the organism deposited as CCUG 47753T and DSM 19118T as the type strain of the species Actinobaculum massiliense do not have the properties given by Greub & Raoult (2002). Based on the absence of an authentic type strain the Judicial Commission is requested to examine the status of the name Actinobaculum massiliense Greub & Raoult 2006 and to issue an Opinion.
    International journal of systematic and evolutionary microbiology. 12/2014;
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    ABSTRACT: A novel halophilic, filamentous actinomycete, designated H254(T), was isolated from a Saharan soil sample collected from Biskra (Northern Sahara), and subjected to a polyphasic taxonomic characterization. The strain is Gram-positive, aerobic, and halophilic, and the optimum NaCl concentration for growth is 15-20 % (w/v). The cell-wall hydrolysate contained meso-diaminopimelic acid, and the diagnostic whole-cell sugars were arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H4) was the predominant menaquinone. The major fatty acid profiles were anteiso-C17:0 (32.8 %), C15:0 (28 %), and iso-C17:0 (12.3 %). Comparative analysis of the 16S rRNA gene sequences revealed that the strain H254(T) formed a well-separated sub-branch within the radiation of the genus Actinopolyspora, and the microorganism was most closely related to Actinopolyspora saharensis DSM 45459(T) (99.2 %), Actinopolyspora halophila DSM 43834(T) (99.1 %), and Actinopolyspora algeriensis DSM 45476(T) (99.0 %). Nevertheless, the strain had relatively lower mean values for DNA-DNA relatedness with the above strains (57.2, 68.4, and 45.6 %, respectively). Based on phenotypic features and phylogenetic position, we propose that strain H254(T) represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora biskrensis sp. nov. is proposed. The type strain of A. biskrensis is strain H254(T) (=DSM 46684(T) =CECT 8576(T)).
    Current microbiology. 11/2014;
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    ABSTRACT: The remarkable host specificity of Actinobaculum species directed our attention to recharacterize these species by a polyphasic approach. A comparative chemotaxonomic study including analysis of whole-cell sugars, amino acid composition of the peptidoglycan, fatty acid methyl esters, respiratory quinones and polar lipids reveals significant differences that, in combination with molecular data, support a dissection of the genus Actinobaculum. The proposals of this study include the reclassification of Actinobaculum schaalii and Actinobaculum urinale as Actinotignum schaalii gen. nov., comb. nov. (type strain DSM 15541T=CCUG 27420T) and Actinotignum urinale comb. nov. (type strain DSM 15805T=CCUG 46093T), respectively. The results of 16S rRNA gene sequence analysis and DNA-DNA hybridization also indicated that the type strain of Actinobaculum massiliense deposited in the CCUG as CCUG 47753T(= DSM 19118T) should in fact be consider a member of the species A. schaalii. In addition, comparative 16S rRNA gene sequencing and DNA-DNA homology studies of four strains recovered from clinical materials demonstrated that three isolates belonged to Actinotignum schaalii, the remaining strain represents a novel species, for which the name Actinotignum sanguinis sp. nov. is proposed. The type strain is IMMIB L-2199T (=DSM 26039T=CCUG 64068T). Copyright © 2014, the Society for General Microbiology.
    International journal of systematic and evolutionary microbiology. 11/2014;
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    ABSTRACT: A gamma radiation-resistant, Gram reaction-positive, aerobic and chemoorganotrophic actinobacterium, initially designated Geodermatophilus obscurus subsp. dictyosporus G-5T, was not validly named at the time of initial publication (1968). G-5T formed black-colored colonies on GYM agar. The optimal growth range was 25–35 °C, at pH 6.5–9.5 and in the absence of NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content of the strain was 75.3 mol %. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid; MK-9(H4) was the dominant menaquinone and galactose was detected as a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids, iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 94.8–98.4 % sequence identity with the members of the genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G-5T is proposed to represent a novel species, Geodermatophilus dictyosporus and the type strain is G-5T (=DSM 43161T = CCUG 62970T = MTCC 11558T = ATCC 25080T = CBS 234.69T = IFO 13317T = KCC A-0154T = NBRC 13317T). The INSDC accession number is HF970584.
    Extremophiles 11/2014; · 2.20 Impact Factor
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    ABSTRACT: A red-pigmented, Gram-reaction-negative, aerobic bacterial strain, designated No.164T, was isolated from sediment sample from the alkaline Lake Elmenteita located in the Kenyan Rift Valley. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Belliella, with the highest sequence similarity (97%) to Belliella pelovolcani DSM 46698T. Optimal growth temperature was 30-35°C, at pH 7.0-12.0 in the presence of 0-4% (w/v) NaCl. Flexirubins were absent. The respiratory menaquinone (MK-7), predominant cellular fatty acids (iso-C15:0, anteiso-C15:0 and a mixture of C16:1ω7c and/or iso-C15:0 2-OH) and DNA G + C content (38.1 mol%) of strain No.164T were consistent with those of other members of the genus Belliella. The polar lipids consisted of phosphatidylethanolamine, eight unspecified lipids and one unspecified phospholipid. Several phenotypic characteristics can be used to differentiate this isolate from those of other Belliella species. The polyphasic data presented in this study indicated that this isolate should be classified to represent a novel species in the genus Belliella. The name Belliella kenyensis sp. nov. is therefore proposed; the type strain is strain No.164T (= DSM 46651 = CECT 8551).
    International journal of systematic and evolutionary microbiology. 11/2014;
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    ABSTRACT: An alkalitolerant actinomycete strain, designated B32(T), was isolated from a Saharan soil sample collected from Adrar province (South of Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 0-5 % (w/v) and the optimum growth temperature and pH were found to be 25-35 °C and 7.0-10.0 °C, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain B32(T) were identified as MK-10 (H4) and MK-11 (H4). The major fatty acids were found to be iso-C16:0 and anteiso-C15:0. The diagnostic phospholipids detected were phosphatidylcholine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The chemotaxonomic properties of strain B32(T) are consistent with those shared by members of the genus Nocardiopsis. 16S rRNA gene sequence analysis indicated that strain B32(T) is most closely related to Nocardiopsis alba DSM 43377(T) (98.7 %), Nocardiopsis lucentensis DSM 44048(T) (98.6 %), Nocardiopsis aegyptia DSM 44442(T) (98.6 %), Nocardiopsis sinuspersici HM6(T) (98.6 %) and Nocardiopsis arvandica HM7(T) (98.5 %). However, the DNA-DNA relatedness values between strain B32(T) and the closely related type strains were 17.9, 14.6, 31.1, 27.1 and 14.1 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain B32(T) should be classified as representative of a novel species, for which the name Nocardiopsis algeriensis sp. nov. is proposed. The type strain is B32(T) (=DSM 45462(T) = CECT 8712(T)).
    Antonie van Leeuwenhoek 11/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258(T)) to 97.8 % (Actinokineospora baliensis NBRC 104211(T)). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25-30 °C and 5.0-7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H4). The major fatty acids were found to be iso-C16:0, iso-C15:0, iso-C16:1 H and iso-C16:0 2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84(T) (=DSM 45961(T) = CECT 8578(T)).
    Antonie van Leeuwenhoek 11/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel marine bacterium, strain LBS2T was isolated from eggs carried on pleopods of the spiny lobster collected from Andaman Sea. Heterotrophic growth occurred at 1–7% NaCl. 16S rRNA gene sequence similarity revealed the strain LBS2T belonged to the genus Vibrio and showed above 97% similarity with eight type strains of the genus Vibrio. Multilocus analysis based on ftsZ, gapA, gyrB, mreB, pyrH recA, rpoA, and topA revealed LBS2T formed a separate cluster with Vibrio ponticus DSM 16217T with 89.8% multilocus gene sequence similarity. However, strain LBS2T is distantly related with other members of the Scophthalmi clade in terms of 16S rRNA signatures, phenotypic variations and multilocus gene sequence similarity, for which we propose LBS2T belongs to a new clade i.e. Ponticus clade with V. ponticus DSM 16217T as the representative type strain of the clade. DNA–DNA homologies between strain LBS2T and closely related strains were well below 70%. DNA G + C content was 45.3 mol%. On the basis of our polyphasic study, strain LBS2T represents a novel species of the genus Vibrio, for which the name Vibrio panuliri sp. nov. is proposed. The type strain is LBS2T (= JCM 19500T = DSM 27724T = LMG 27902T).
    Research in Microbiology. 11/2014;
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    ABSTRACT: A halophilic actinomycete, strain R4S8T was isolated from soil of Inche-Broun hypersaline wetland in the north of Iran. The isolate grew aerobically at temperatures of 30-50 °C (optimum temperature 40 °C), pH 6-10 (optimum pH 7.0) and NaCl concentrations 1-15 % w/v (optimum 3-5 %). It formed short and straight to moderately flexuous aerial mycelium without motile elements. The cell wall of strain R4S8T contained meso-diaminopimelic acid as diaminoacid without any diagnostic sugars. The polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyl serine and phosphatidylmonomethylethanolamine. It synthesized anteiso-C15:0 (44.8 %), iso-C15:0 (28.8 %) and iso-C14:0 (8.5 %) as major fatty acids. MK-6 was the predominant respiratory quinone. The G+C content of the genomic DNA was 52.6 mol %. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain R4S8T belongs to the family Thermoactinomycetaceae and showed the closest phylogenetic similarity with Desmospora activa IMMIB L-1269T (95.5 %) and Marininema mesophilum SCSIO 10219T (95.3 %). On the basis of phylogenetic analysis and phenotypic characteristics, R4S8T represents a novel species in a new genus within the family Thermoactinomycetaceae, for which the name Salinithrix halophila gen. nov., sp. nov. is proposed. The type strain of Salinithrix halophila is R4S8T (= IBRC-M 10813T = CECT 8506T).
    International journal of systematic and evolutionary microbiology. 09/2014;
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    ABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) protein analysis, automated ribotyping, and phenotypic tests (e.g., cell morphology, gas production from glucose, growth and acid production on homofermemtative-heterofermentative differential (HHD) agar medium, sugar fermentation patterns) were used to identify 23 lactic acid bacteria (LAB) isolated from fermented cereal foods available in Abidjan, Côte d'Ivoire. Pediococcus acidilactici (56.5%), Lactobacillus fermentum (30.4%), L. salivarius (4.3%), P. pentosaceus (4.3%) and L. plantarum subsp. plantarum (4.3%) were the species and subspecies identified. Protein based identification was confirmed by automated ribotyping for selected isolates and was similar to that provided by the phenotypic characterization. MALDI-TOF MS protein analysis provided a high level of discrimination among the isolates and could be used for the rapid screening of LAB starter cultures.
    The Open Microbiology Journal 09/2014; 8:78-86.
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    ABSTRACT: Three halophilic mycelium-forming actinobacteria, strains H195(T), H150 and H151, were isolated from a Saharan soil sample collected from Béni-isguen in the Mzab region (Ghardaïa, South of Algeria) and subjected to a polyphasic taxonomic characterisation. These strains were observed to show an aerial mycelium differentiated into coccoid spore chains and fragmented substrate mycelium. Comparative analysis of the 16S rRNA gene sequences revealed that the highest sequence similarities were to Saccharopolyspora qijiaojingensis YIM 91168(T) (92.02 % to H195(T)). Phylogenetic analyses showed that the strains H195(T), H150 and H151 represent a distinct phylogenetic lineage. The cell-wall hydrolysate was found to contain meso-diaminopimelic acid, and the diagnostic whole-cell sugars were identified as arabinose and galactose. The major cellular fatty acids were identified as iso-C15:0, iso-C16:0, iso-C17:0 and anteiso-C17:0. The diagnostic phospholipid detected was phosphatidylcholine and MK-9 (H4) was found to be the predominant menaquinone. The genomic DNA G+C content of strain H195(T) was 68.2 mol%. On the basis of its phenotypic features and phylogenetic position, we propose that strain H195(T) represents a novel genus and species, Mzabimyces algeriensis gen. nov., sp. nov., within a new family, Mzabimycetaceae fam. nov. The type strain of M. algeriensis is strain H195(T) (=DSM 46680(T) = MTCC 12101(T)).
    Antonie van Leeuwenhoek 09/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel Gram-staining-negative, aerobic, non-endospore-forming, non-pigmented, rod shaped, slightly moderately halophilic bacterium, designated GBPy5T was isolated from aquatic plants in Gomishan wetland, Iran. Cells of strain GBPy5T were motile. Growth occurred between 1-10 % (w/v) NaCl and the isolate grew optimally at 3 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 8.0 and 30 oC, respectively, while it was able to grow over a pH range of 6.5-9.0 and a temperature range of 4-35 oC. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GBPy5T is a member of the genus Pseudomonas forming a monophyletic branch. The novel strain exhibited a 16S rRNA gene sequence similarity of 95.4 % with the type strains of Pseudomonas guariconensis PCAVU11T and P. sabulinigri J64T, respectively. The major cellular fatty acids of the isolate were C18:1 ω7c (37.8 %), C16:0 (14.9 %), C16:1 ω7c (12.9 %), C12:0 3OH (7.1 %) and C12:0 (7.0 %). The polar lipids pattern of strain GBPy5T consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The G+C content of the genomic DNA of this strain was 59.2 mol%. On the basis of the phenotypic and phylogenetic data, strain GBPY5T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salegens sp. nov. is proposed. The type strain is strain GBPy5T (= IBRC-M 10762T= CECT 8338T).
    International journal of systematic and evolutionary microbiology. 07/2014;
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    ABSTRACT: A novel Gram-staining-positive, moderately halophilic bacterium, designated strain B6BT, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain B6BT were rod-shaped, motile and producing ellipsoidal endospores at a terminal position in non-swollen sporangia. Strain B6BT was a strictly aerobic bacterium and catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5-20.0 % (w/v), with optimum growth occurring at 10.0 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain B6BT was shown to belong to the phylum Firmicutes and the closest phylogenetic similarities were with the species Virgibacillus koreensis BH30097T (97.5 %), Virgibacillus albus YIM 93624T (97.4 %), Sediminibacillus halophilus EN8dT (96.8 %), Sediminibacillus albus NHBX5T (96.6 %), Virgibacillus carmonensis LMG 20964T (96.3 %) and Paraliobacillus quinghaiensis YIM-Ci58T (96.0 %), respectively. Phylogenetic analysis revealed that strain B6BT along with Virgibacillus koreensis BH30097Tand Virgibacillus albus YIM 93624Tclustered in a separate clad in the family Bacillaceae. The DNA G+C content of this new isolate was 35.8 mol %. DNA-DNA hybridization experiments revealed low levels of relatedness between strain B6BTand Virgibacillus koreensis BH30097T (13 %) and Virgibacillus albus (33 %).The major cellular fatty acid of strain B6BT was anteiso-C15:0 (75.1 %) and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipids and an unknown glicolipid. The isoprenoid quinones were MK-7 (90 %) and MK-6 (3 %). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features support the placement of isolate B6BT within Firmicutes, closely related to Virgibacillus koreensis and Virgibacillus albus but with features that clearly distinct it from those of the genus Virgibacillus or other related genera. On the basis of polyphasic evidence from this study, we propose the placement of strain B6BTwithin a new genus, as Aquibacillus halophilus gen. nov., sp. nov., with B6BT as type strain (=IBRC-M 10775T= KCTC 13828T) and the transfer of Virgibacillus koreensis and Virgibacillus albus to this new genus, as Aquibacillus koreensis comb. nov. and Aquibacillus albus comb. nov., respectively. The type strain of Aquibacillus koreensis is BH30097T (=KCTC 3823T=IBRC-M10657T=JCM 12387T) and the type strain of Aquibacillus albus is YIM 93624T (= DSM23711T= IBRC-M10798T= JCM 17364T).
    International journal of systematic and evolutionary microbiology 07/2014; · 2.11 Impact Factor
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    ABSTRACT: A novel Gram-staining-negative, motile, non-pigmented, facultative anaerobe, spirillum-shaped, halophilic and alkaliphilic bacterium, designated strain GCWy1T, was isolated from water of the coastal-marine wetland Gomishan in Iran. The strain was able to grow at NaCl concentrations of 1-10 % (w/v) and optimal growth was achieved at 3 % (w/v). The optimum pH and temperature for growth were pH 8.5 and 30 °C, while it was able to grow at pH 7.5-10 and 4-40 °C. Phylogenetic analysis based on the comparison of the 16S rRNA gene sequence placed the new isolate within the Gammaproteobacteria as a separate deep branch, with 92.1 % or lower sequence similarity to representatives of the genera Saccharospirillum and Reinekea and less than 91.0 % sequence similarity with other remotely related genera. The major cellular fatty acids of the isolate were C18:1ω7c, C16:0 and C17:0, and its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The cells of strain GCWy1T contained the isoprenoid quinones Q-9 and Q-8 (81 %:2 %). The G+C content of the genomic DNA of this strain was 52.3 mol %. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and phenotypic data, strain GCWy1T represents a novel species in a new genus in the family Saccharospirillaceae, order Oceanospirillales, for which the name Salinispirillum marinum gen. nov., sp. nov. is proposed. The type strain of the type species is GCWy1T (= IBRC-M 10765T = CECT 8342T).
    International journal of systematic and evolutionary microbiology 07/2014; · 2.11 Impact Factor
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    ABSTRACT: Nocardia species are ubiquitous in the environment with an increasing number of species isolated from clinical sources. From 2005 to 2009, eight isolates (W9042, W9247, W9290, W9319, W9846, W9851(T), W9865, and W9908) were obtained from eight patients from three states in the United States and Canada; all were from males ranging in age from 47 to 81 years old; and all were obtained from finger (n = 5) or leg (n = 3) wounds. Isolates were characterized by polyphasic analysis using molecular, phenotypic, morphologic and chemotaxonomic methods. Sequence analysis of 16S rRNA gene sequences showed the eight isolates are 100 % identical to each other and belong in the genus Nocardia. The nearest phylogenetically related neighbours were found to be the type strains for Nocardia altamirensis (99.33 % sequence similarity), Nocardia brasiliensis (99.37 %), Nocardia iowensis (98.95 %) and Nocardia tenerifensis (98.44 %). The G+C content of isolate W9851(T) was determined to be 68.4 mol %. The DNA-DNA relatedness between strain W9851(T) and the N. brasiliensis type strain was 72.8 % and 65.8 % when measured in the laboratory and in silico from genome sequences, respectively, and 95.6 % ANI. Whole-cell peptidoglycan was found to contain meso-diaminopimelic acid; MK-8-(H4)ω-cyc was identified as the major menaquinone; the major fatty acids were identified as C16:0, 10 Me C18:0, and C18:1 w9c, the predominant phospholipids were found to include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; whole-cell sugars detected were arabinose and galactose; and mycolic acids ranging from 38 to 60 carbon atoms were found to be present. These chemotaxonomic analyses are consistent with assignment of the isolates to the genus Nocardia. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra of the clinical isolates showed genus and species level profiles that were different from other Nocardia species. All isolates were resistant to ciprofloxacin, clarithromycin and imipenem but were susceptible to amikacin, amoxicillin/clavulanate, linezolid and trimethoprim/sulfamethoxazole. The results of our polyphasic analysis suggest the new isolates obtained from wound infections represent a novel species within the genus Nocardia, for which the name Nocardia vulneris sp. nov. is proposed, with strain W9851(T) (= DSM 45737(T) = CCUG 62683(T) = NBRC 108936(T)) as the type strain.
    Antonie van Leeuwenhoek 07/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel Promicromonospora strain, designated HM 792T, was isolated from soil in Fars Province, Iran. On ISP 2 medium, the yellow-pigmented isolate produced long and branched hyphae that developed into a large number of irregular shaped spores. It showed optimal growth at 25-30 °C and pH 6.0-9.0 with 0-8 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Promicromonospora. Whole cell hydrolyzates of strain HM 792T contained amino acids D-glutamic acid, L-alanine and L-lysine along with sugars glucose and ribose. The main polar lipids were diphosphatidylglycerol, two unknown phospholipids, two unknown glycolipids and two unknown glycophospholipids, complemented by minor concentrations of phosphatidylinositol and phosphatidylglyserol. MK-9(H4) was the predominant menaquinone. The fatty-acid pattern was mainly composed of the saturated branched-chain acids anteiso-C15:0 and iso-C15:0. The 16S rRNA gene sequence analysis showed the highest pairwise sequence identity (96.6-99.0 %) with the members of the genus Promicromonospora. Based on phenotypic and genotypic features, strain HM 792T (DSM 45554T = UTMC00792T = CCUG 63022T) was considered to represent a novel species of the genus Promicromonospora, for which the name Promicromonospora iranensis is proposed.
    International journal of systematic and evolutionary microbiology. 07/2014;
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    ABSTRACT: A Gram-reaction-positive bacterial isolate, designated Tü 6233T, with rudimentary and coral pink vegetative mycelium that did neither form aerial mycelia nor spores was isolated from a Brazilian soil sample. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. Cell wall hydrolysates contained meso-diaminopimelic acid as diagnostic diamino acid and galactose as diagnostic sugar. Major fatty acids were iso-C16:0, iso-C15:0 and C17:1ω8c and the predominant menaquinone was MK-9(H4). Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, an unknown glycophospholipid and an unknown phospholipid. The DNA G+C content of the strain was 75.4 mol %. The 16S rRNA gene sequence identity with members of the genus Geodermatophilus was 94.2-98.7 %. Based on phenotypic, chemotaxonomic and phylogenetic data strain Tü 6233T is proposed to represent a novel species, Geodermatophilus brasiliensis, with the type strain Tü 6233T (DSM 44526T = CECT 8402T).
    International journal of systematic and evolutionary microbiology 05/2014; · 2.11 Impact Factor
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    ABSTRACT: A halophilic actinomycete strain, designated H27(T), was isolated from a soil sample collected from a hypersaline habitat in Djelfa Province (North-Central Algeria), and then investigated using a polyphasic taxonomic approach. The strain was observed to produce poor aerial mycelium, which formed short chains of oval to cylindrical-shaped spores at maturity, and non fragmented substrate mycelium. The optimum NaCl concentration for growth was found to be 10-15 % (w/v) and the optimum growth temperature and pH were found to be 28-37 °C and 6-7, respectively. The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinones of strain H27(T) were identified as MK-11 (H4) and MK-10 (H6). The major fatty acids were found to be iso-C16:0, anteiso-C17:0, 10 methyl C17:0 and 10 methyl C16:0. The diagnostic phospholipids detected were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The chemotaxonomic properties of strain H27(T) are consistent with those shared by members of the genus Streptomonospora. 16S rRNA gene sequence analysis indicated that strain H27(T) is most closely related to Streptomonospora alba DSM 44588(T) (98.8 %) and Streptomonospora flavalba DSM 45155(T) (98.7 %) whereas the DNA-DNA relatedness values between strain H27(T) and the two type strains were 17.1 and 57.9 %, respectively. Based on the combined genotypic and phenotypic evidence, it is proposed that strain H27(T) should be classified as representative of a novel species, for which the name Streptomonospora algeriensis sp. nov. is proposed. The type strain is H27(T) (=DSM 45604(T) =CCUG 63369(T) =MTCC 11563(T)).
    Antonie van Leeuwenhoek 05/2014; · 2.07 Impact Factor
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    ABSTRACT: A novel non-motile, Gram-staining-negative, yellow-pigmented bacterium, designated CT348(T), isolated from the ectorhizosphere of an organic olive tree in Spain and characterised as an efficient plant growth promoting bacterium, was investigated to determine its taxonomic status. The isolate grew best in a temperature range of 5-35°C, at pH 5.0-8.0 and with 0-1% (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Chryseobacterium. The DNA G+C content of the novel strain was 38.2mol%. The strain contained a polyamine pattern with sym-homospermidine as the major compound and produced flexirubin-type pigments. MK-6 was the dominant menaquinone and the major cellular fatty acids were iso-C15:0, C17:1ω9c, iso-C17:0 3-OH and iso-C15:0 2-OH. The main polar lipids were phosphatidylethanolamine and several unidentified lipids and aminolipids. The 16S rRNA gene showed 92.2-97.8% sequence identity with the members of the genus Chyseobacterium. Based on the phenotypic traits and DNA-DNA hybridizations with the type strains of the most closely related species, the isolate is shown to represent a novel species, Chyseobacterium oleae, type strain CT348(T) (=DSM 25575 =CCUG 63020). Emended descriptions of the genus Chryseobacterium and C. daecheongense, C. gambrini, C. gleum, C. joostei, C. jejuense, C. luteum, C. shigense, C. taiwanense, C. ureilyticum and C. vrystaatense are also proposed.
    Systematic and Applied Microbiology 05/2014; · 3.29 Impact Factor
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    ABSTRACT: A novel Gram-staining-positive, moderately halophilic bacterium, designated strain A76T, was isolated from the brine sample of the hypersaline lake Aran-Bidgol in Iran. Cells were strictly aerobic, coccus-shaped, non-motile, non-sporulating, catalase and oxidase positive. Strain A76T grew between pH 7.0-10.0 (optimal growth at pH 8.0), between 20-45°C (optimal growth at 35°C) and at salinities of 0.5 to 12.5% (w/v) NaCl (optimal growth at 7.5% [w/v] NaCl). On the basis of 16S rRNA gene sequence analysis, strain A76T was shown to belong to the phylum Firmicutes with sequence similarities of 94.1%, 93.1% and 91.1%, to the type species of the genera Jeotgalicoccus, Salinicoccus and Nosocomiicoccus, respectively. The DNA G+C content of this new isolate was 38.8 mol%. The major cellular fatty acids of strain A76T were anteiso-C15:0 and iso-C15:0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, an unknown lipid and two unknown phospholipids. The isoprenoid quinones were MK-6 (94%), MK-5 (3%) and MK-7 (3%). The amino acid constituents of the cell wall were Lys, Asp, Gly, Glu and Ala. The physiological, biochemical and phylogenetic differences between strain A76T and type strains of validly named taxa suggest that this strain represents a new species in a novel genus within the Staphylococcaceae, for which the name Aliicoccus persicus gen. nov., sp. nov. is proposed. The type strain of Aliicoccus persicus is strain A76T (=CECT 8508T =DSM 28306T =IBRC-M 10081T).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2014; · 2.11 Impact Factor

Publication Stats

5k Citations
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Institutions

  • 1993–2014
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2012–2013
    • École Normale Supérieure de Kouba
      Mastaganeam, Mostaganem, Algeria
    • Carl von Ossietzky Universität Oldenburg
      • Department of Chemistry and Biology of the Marine Environment (ICBM)
      Oldenburg, Lower Saxony, Germany
    • Macquarie University
      Sydney, New South Wales, Australia
  • 2011–2013
    • University of Tehran
      • • Department of Microbiology (VM)
      • • School of Biology
      Tehrān, Ostan-e Tehran, Iran
    • Institute for Agricultural and Fisheries Research
      Meirelbeke, Flanders, Belgium
  • 2009–2013
    • University of Delhi
      • Department of Zoology (Faculty of Science)
      Delhi, NCT, India
    • Mugla Üniversitesi
      Mobolla, Muğla, Turkey
    • National Institute for Biotechnology and Genetic Engineering
      Shah Faisalabad, Punjab, Pakistan
    • National Academy of Agricultural Science (South Korea)
      Sŏul, Seoul, South Korea
    • University of Haifa
      • Department of Evolutionary and Environmental Biology
      H̱efa, Haifa District, Israel
    • University of Innsbruck
      • Institut für Mikrobiologie
      Innsbruck, Tyrol, Austria
  • 2003–2013
    • Eötvös Loránd University
      • • Department of Microbiology
      • • Department of Genetics
      Budapest, Budapest fovaros, Hungary
  • 2009–2012
    • Justus-Liebig-Universität Gießen
      • Institut für Angewandte Mikrobiologie
      Gießen, Hesse, Germany
  • 2002–2012
    • University of Coimbra
      • • Departamento de Ciências da Vida
      • • Centro de Neurociências e Biologia Celular (CNC)
      Coimbra, Distrito de Coimbra, Portugal
    • Pacific Institute of Bioorganic Chemistry
      Wladiwostok, Primorskiy, Russia
  • 2010–2011
    • University of Bonn
      • Institute of Medical Microbiology, Immunology and Parasitology
      Bonn, North Rhine-Westphalia, Germany
    • Centers for Disease Control and Prevention
      Atlanta, Michigan, United States
  • 2007–2011
    • Universidade Católica Portuguesa
      • Escola Superior de Biotecnologia
      Lisbon, Lisbon, Portugal
  • 2004–2011
    • Ghent University
      • Laboratory of Microbiology
      Gand, Flanders, Belgium
    • French National Centre for Scientific Research
      Lutetia Parisorum, Île-de-France, France
    • Alexandria University
      • Department of Microbiology (Faculty of Veterinary Medicine)
      Alexandria, Alexandria, Egypt
    • University of Reading
      • Food and Nutritional Sciences
      Reading, England, United Kingdom
  • 2001–2011
    • Humboldt-Universität zu Berlin
      • Department of Biology
      Berlin, Land Berlin, Germany
    • Leibniz Centre for Agricultural Landscape Research
      Muencheberg, Brandenburg, Germany
    • University of Milan
      • Department of Food Science and Microbiology DISTAM
      Milano, Lombardy, Italy
    • La Trobe University
      Melbourne, Victoria, Australia
  • 2006–2009
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • Biological Resource Center
      Anzan, Gyeonggi Province, South Korea
    • Centro de Investigaciones Biológicas del Noroeste
      La Paz, Baja California Sur, Mexico
  • 2003–2009
    • Yunnan University
      • • The Key Laboratory for Microbial Resources of the Ministry of Education
      • • Yunnan Institute of Microbiology and Laboratory for Conservation and Utilization of Bio-Resources
      Yün-nan, Yunnan, China
  • 2008
    • Marquette University
      Milwaukee, Wisconsin, United States
  • 2007–2008
    • Institute of Agricultural Sciences
      Vārānasi, Uttar Pradesh, India
  • 2006–2008
    • Tampere University of Technology
      • Department of Chemistry and Bioengineering
      Tampere, Western Finland, Finland
  • 2004–2008
    • Universidad de Salamanca
      • Departamento de Microbiología y Genética
      Salamanca, Castile and Leon, Spain
  • 2002–2008
    • University of Veterinary Medicine in Vienna
      • Institute of Bacteriology, Mycology and Hygiene
      Wien, Vienna, Austria
  • 2000–2008
    • Università degli Studi di Messina
      • Dipartimento di Scienze Biologiche e Ambientali
      Messina, Sicily, Italy
    • Spanish National Research Council
      • Institute for Natural Resources and Agrobiology of Sevilla
      Madrid, Madrid, Spain
  • 1999–2008
    • Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
      Jena, Thuringia, Germany
  • 2006–2007
    • Indian Agricultural Research Institute
      • Division of Nematology
      New Delhi, NCT, India
  • 2005–2007
    • University of Manitoba
      • Department of Microbiology
      Winnipeg, Manitoba, Canada
  • 2003–2007
    • Université de Bretagne Occidentale
      Brest, Brittany, France
  • 2002–2005
    • Bundesanstalt für Geowissenschaften und Rohstoffe
      Hanover, Lower Saxony, Germany
    • Russian Academy of Sciences
      • Institute of Microbiology
      Moscow, Moscow, Russia
  • 2002–2003
    • University of Vienna
      Wien, Vienna, Austria
  • 1999–2000
    • Christian-Albrechts-Universität zu Kiel
      • Institute for General Microbiology
      Kiel, Schleswig-Holstein, Germany