ABSTRACT: The goal of this study was to determine the content of such biological active metals as Se, Hg, Al, Cu, Zn, As, Cd, Pb, Mg, Ca and Fe in Boletus Edulis mushrooms and to study the effect of mushrooms as inhibitors of blood serum copper-initiated lipid peroxidation. The metals content was determined by ICP – OES technique and blood lipids peroxidation in vitro was assessed by thiobarbituric acid-reactive substances measurement. The dependency between quality and content of the determined biological active metals has been traced. Samples were analyzed of wild growing mushrooms Boletus Edulis from two mountain regions in Bulgaria. On the average the content of Se in Boletus Edulis was found to be 25 mg/kg dried mushroom, this content being higher in tubules than in fleshy part. We found that Boletus Edulis mushrooms inhibited lipid peroxidation in the concentration dependent manner. The effective concentration of Boletus Edulis is in 5 times lower compare to the concentration of Cantharellus Cibarius resulting in similar lipid peroxidation inhibition. This effect can be explained by 56 times higher content of Se and by 1.5 and 3 times lower content of such initiators of lipid peroxidation as Cu and Fe in Boletus Edulis compare to Cantharellus Cibarius.
A system with a source of infrared radiation heating, developed by authors, was used for the mushroom mineralization. We conclude that Boletus Edulis is an effective inhibitor of blood lipid peroxidation and in 5 times more stronger rather than Cantharellus Cibarius.
4th International Symposium "Recent advances in food analysis" (RAFA), Prague, Czech, 4-6 November, 2009; 01/2009