Norio Hayashi

Publications (2)5.7 Total impact

• Article: Expression of DNA methyltransferase in LEC rats during hepatocarcinogenesis

  Eiji Miyoshi, Suresh K. Jain, Toshihiro Sugiyama, Junichi Fujii, Norio Hayashi, Hideyuki Fusamoto, Takenobu Kamada, Naoyuki Taniguchi
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  ABSTRACT: The Long-Evans with a cinnamon-like color (LEC) rat is a mutant of the Long-Evans strain that develops hereditary hepatitis and hepatoma with ageing. Age-related changes in the mRNA expression of DNA methyltransferase (DNA MTase) were examined in livers of LEC rats using Long Evans with an agouti color (LEA) rats as controls. A dramatic increase in the expression of this mRNA was observed in LEC rats at 20 weeks when acute hepatitis appeared. Their high mRNA levels were maintained until 52 weeks of age. The mRNA expression as well as DNA MTase activities were found to be higher in cancer lesions than in adjacent normal tissue. These increases may be related to liver regeneration and to early events in cellular transformation of LEC rats.
  Carcinogenesis 05/1993; · 5.70 Impact Factor
• Article: High expression of an N-acetylglucosaminyltransferase III in 3′-methyl DAB-induced hepatoma and ascites hepatoma

  Atsushi Nishikawa, Shigeru Fujii, Toshihiro Sugiyama, Norio Hayashi, Naoyuki Taniguchi
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  ABSTRACT: Ascites hepatoma AH-66 and 3′-Me-DAE-induced hepatoma of rats contain highly active N-acetylglucosaminyltransferase III (GnT-III) which catalyzes the addition of N-acetylglucosamine through a β1-4 linkage (bisecting N-acetylglucosamine) to the β-linked mannose of the trimannosyl core of asparagine-linked sugar chains, whereas normal rat liver contains very little. The high activity was also detected in fetal rat liver, newborn rat liver, hyperplastic nodules and various transplantable hepatomas.
  Biochemical and Biophysical Research Communications.