Min Wang

Huazhong University of Science and Technology, Wu-han-shih, Hubei, China

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Publications (158)240.34 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In order to enhance the charge-transfer ability, positively charged polyaniline-graphene complexes are synthesized by a reflux method and assembled into multilayers with negatively charged graphene oxide. The counter electrodes from polyaniline-graphene/graphene oxide (PANi-G/GO)n (n represents the bilayer number) multilayers show superior electrocatalytic activity and electrical conductivity because of enormous interface. An impressive power conversion efficiency of 7.88% is recorded from the DSSC employing PANi-G/GO multilayer counter electrode. The multilayer counter electrodes and the resultant DSSCs are thoroughly assessed by electrochemical characterizations. The results are far from optimal but the preliminary photovoltaic performances make the strategy promising in efficient DSSC applications.
    Electrochimica Acta 08/2014; 137:175–182. · 4.09 Impact Factor
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    ABSTRACT: This study aimed to investigate the role in metastasis and prognostic value of KAP-1 in pancreatic cancer (PC). The expression of KAP-1 was analyzed by quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemical staining in 91 human PC tissue samples. Capan-2 cells were transfected with a lentiviral vector expressing KAP-1 (Capan-2/KAP-1) or the empty vector (Capan-2/vector); cell migration and invasion were assayed in vitro using Transwell migration and wound-healing assays, and in vivo using a xenograft model in nude mice. KAP-1 was found to be overexpressed in human PC, and the expression of KAP-1 correlated with clinical stage. Overexpression of KAP-1 increased the invasion and migration of Capan-2 cells in vitro. Furthermore, overexpression of KAP-1 promoted the growth and metastatic ability of PC cells in a xenograft model in nude mice. Moreover, overexpression of KAP-1 induced the epithelial-mesenchymal transition (EMT) in PC cells both in vitro and in vivo, as indicated by increased expression of mesenchymal markers such as vimentin and decreased expression of E-cadherin. This study indicates that KAP-1 may promote metastasis in PC by regulating the EMT and suggests that KAP-1 may have potential as a predictor of metastasis in patients with pancreatic cancer.
    Medical oncology (Northwood, London, England). 07/2014; 31(7):25.
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    ABSTRACT: With an aim of accelerating the charge transfer between polyaniline (PANi) and graphene, polyaniline-graphene (PANi-graphene) complexes are synthesized by a reflux technique and employed as counter electrodes (CEs) for dye-sensitized solar cells (DSSCs). Owing to the easy charge-transfer between PANi (N atoms) and graphene (C atoms) by a covalent bond, electrical conduction and electrocatalysis of PANi-graphene complex CEs, and therefore power conversion efficiency of their DSSCs have been elevated in comparison with that of PANi-only CE. The resultant PANi-graphene complex CEs are characterized by spectral analysis, morphology observation, and electrochemical tests. The DSSC employing PANi-8 wt‰ graphene complex CE gives an impressive power conversion efficiency of 7.78%, which is higher than 6.24% from PANi-only and 6.52% from Pt-only CE based DSSCs.
    ACS Applied Materials & Interfaces 05/2014; · 5.90 Impact Factor
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    ABSTRACT: EphB2 is an important member of the receptor tyrosine kinases. Recently, EphB2 was shown to facilitate T-cell migration and monocyte activation. However, the effects of EphB2 on B cells remain unknown. In this study, the expression of EphB2 on B cells was tested by Western blot, and the roles of EphB2 in B-cell proliferation, cytokine secretion, and immunoglobulin (Ig) production were evaluated using EphB2 siRNA interference in human B cells from healthy volunteers. Our study revealed that EphB2 was distributed on naive B cells and was up-regulated on activated B cells. Moreover, B-cell proliferation (decreased by 22%, P<0.05), TNF-α secretion (decreased by 40%, P<0.01) and IgG production (decreased by 26%, P < 0.05) were depressed concordantly with the down-regulated EphB2 expression. Subsequently, we screened microRNAs that could regulate EphB2 expression in B cells, and discovered that miR-185 directly targeted to EphB2 mRNA and suppressed its expression. Furthermore, miR-185 overexpression inhibited B-cell activation, and the inhibitor of miR-185 enhanced B-cell activation. Moreover, abatement of EphB2 through miR-185 mimics or EphB2 siRNA attenuated the activation of Src-p65 and Notch1 signaling pathways in human B cells. Our study first suggested that EphB2 was involved in human naive B cell activation through Src-p65 and Notch1 signaling pathways and could be regulated by miR-185.-Yu, M., Liang, W., Wen, S., Zhao, T., Zhu, M.-X., Li, H.-H., Long, Q., Wang, M., Cheng, X., Liao, Y.-H., Yuan, J. EphB2 contributes to human naive B-cell activation and is regulated by miR-185.
    The FASEB Journal 05/2014; · 5.70 Impact Factor
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    ABSTRACT: Endothelial microparticles (EMPs) are small vesicular structures that serve as a marker of endothelial function. Angiotensin II receptor type 1 autoantibody (AT1-AA) can cause endothelial dysfunction. However, whether AT1-AA promotes EMPs formation and the mechanism remains obscure. The titres of sera AT1-AA of 126 hypertensive patients and 30 normotensive individuals were evaluated by ELISA. EMPs in the sera and the supernatants of human umbilical vein endothelial cells (HUVECs) were measured by flow cytometry. The phosphorylation levels of mitogen-activated protein kinase (MAPK) pathways in HUVECs treated by AT1-AA were assessed and their correlation with microparticle formation was also analysed. Furthermore, the production of intracellular reactive oxygen species (ROS) and nitric oxide in HUVECs was examined after incubation with 'injured' endothelial microparticle (iEMPs) (EMPs derived from AT1-AA treated HUVECs). The positive rate of AT1-AA in 126 hypertensive patients was 21.4% (27/126), and higher than that in normotensive individuals [3.3% (1/30), P < 0.01]. Circulating EMP (CD31+/CD42b-) levels were corresponding to the AT1-AA titres in hypertensive group (r = 0.3661, P < 0.01). AT1-AA promoted EMPs generation from HUVECs in a time and dose-dependent manner than the vehicle or nonspecific IgG. Meanwhile, AT1-AA significantly elevated phosphorylation level of P38 and ERK in HUVECs. Lorsartan and P38 inhibitor could suppress the AT1-AA's stimulation effect on EMPs generation. Moreover, the iEMP greatly increased ROS production and reduced nitric oxide synthesis in HUVECs. Our findings showed that circulating EMPs levels positively correlate to the serum AT1-AA titres in essential hypertension patients. The AT1-AA could promote EMPs generation in HUVECs through activation of P38 MAPK signalling pathway, and this effect could be effectively inhibited by losartan or p38 inhibitor. Angiotensin receptor blockers (ARBs) may be more suitable for AT1-AA(+) hypertensive patients on account of suppressing the AT1-AA's stimulation effect on EMPs generation.
    Journal of Hypertension 04/2014; 32(4):762-770. · 4.22 Impact Factor
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    ABSTRACT: Background & objectives: a0 ngiotensin II receptor type 1 (AT1) is known to be involved in the pathogenesis of hypertension. t0 his study was undertaken to explore the effect of active immunization against AT1 receptor on blood pressure and small artery remodelling in spontaneously hypertensive rat (SHR). Methods: Male SHR and Wistar rats aged two months were actively immunized with different peptides (ATR12185ͱͲATR10014 and ATR12181) corresponding to particular sequences of rat AT1 receptor, while another SHR group was given losartan (10 mg/kg/day) orally once a day. Anti-AT1 receptor antibodies were detected by ELISA and blood pressure was measured. The effect of the antibodies on the artery and vascular smooth muscle cells (VSMCs) proliferation was studied. Results: all immunized animals produced antibodies against the particular peptides. The systolic blood pressure was decreased in the SHR immunized with peptide-ATR12181 compared with the control. However, no changes were observed in the SHR immunized with other two peptides. The Wistar rats immunized with the three peptides did not show any changes in blood pressure. The media/lumen area ratio of the mesenteric artery was reduced in SHR immunized with ATR12181 and similar to that of the SHR treated with losartan. The antibody from SHR immunized with ATR12181 had no effect on the proliferation of VSMC. But it could inhibit the proliferation caused by angiotensin II and its effect at the titre of 1:40 was similar to that of 1µmol/l losartan. Interpretation & conclusions : Our findings demonstrated that the antibody from SHR immunized with ATR12181 had the effect of reducing blood pressure and target organ protection similar to losartan. Active immunization against AT1 receptor may be a promising strategy in future for the treatment of hypertension.
    The Indian journal of medical research. 04/2014; 139(4):619-24.
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    ABSTRACT: Layer-by-layer (LbL) assembly provides a facile approach in fabricating well-defined nanoarchitectures with electrical and electrochemical properties. Research on electrical and electrochemical performances of the LbL membranes from graphene oxide would be profound in modern photovoltaic nanodevices. We demonstrate here the LbL self-assembly of polyaniline-(graphene oxide/polyaniline)n [PANi-(GO/PANi)n] (n represents the deposition cycle) multilayer films with good electrical and electrochemical behaviors and employment as counter electrodes (CEs) in dye-sensitized solar cells (DSSCs). The resulting multilayer films give a linear growth in bulk conductivity and increased electrocatalytic activity toward triiodides. Careful examination of data and characterizations indicates that the electrical and electrochemical properties are impressive to fulfill the task of PANi-(GO/PANi)n CEs. A promising power conversion efficiency of 7.41% (measured under standard AM1.5 test conditions) is obtained in PANi-(GO/PANi)4 CE in comparison with 6.37% from PANi-(GO/PANi)1 CE. This strategy provides new opportunity for the fabrication of efficient DSSCs, and the efficiency record is expected to be surpassed by scalable technique and components.
    Electrochimica Acta 03/2014; 121:136–142. · 4.09 Impact Factor
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    ABSTRACT: A new concept of synthesizing hexagonal polyaniline (PANi) fibers with polyacrylamide (PAAm) pendants in PAAm oligomer (oligo-PAAm) colloid is proposed. The size and morphology of the composite fibers can be controlled by adjusting the synthesis conditions. A possible growth mechanism of the PANi microstructures based on typical PAAm–crystals interactions in a mild aqueous solution is proposed. The amide ligands decorated oligo-PAAm chains are able to coordinate with –C6H4–NH+C6H4NH+–C6H4–NH–C6H4–NH– sections, leading to a lower surface energy and growth along c-directions to form fibriform morphology. The fibers have uniform diameter ranging from 200 to 400 μm, and length varying from several millimeters to 6 cm. Fourier transform infrared measurement indicates that the fiber exhibits a main PANi chain with PAAm pendants, and ultraviolet–visible spectra reveal that the PANi is in high conductive emeraldine state. Acrylamide feed significantly affects the morphology, however, the conductivity and yield of the fiber depend on hydrochloric acid and potassium peroxydisulfate concentrations in the preparation process. The hexagonal composite fibers show an electrical conductivity of 2.2 S·cm−1 and yield of higher than 95%. Polymer-directed crystal growth may provide promising routes to rational synthesis of various ordered organic materials with complex form and structural specialization. POLYM. COMPOS., 35:253–262, 2014. © 2013 Society of Plastics Engineers
    Polymer Composites 02/2014; 35(2). · 1.48 Impact Factor
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    ABSTRACT: With an aim of significantly enhancing charge-transfer ability of counter electrodes and therefore photovoltaic performances of dye-sensitized solar cells (DSSCs), here we pioneerly report the complexation of polyaniline (PANi) and graphene as well as their employment as counter electrodes (CEs) in efficient DSSCs. Owing to the covalent bond between PANi (N atoms) and graphene (C atoms), charge transfer kinetics is dramatically elevated, which can be confirmed by the enhancement on electrocatalytic activity toward triiodides and a decrease in charge-transfer resistance. A power conversion efficiency of 7.70% is determined from DSSC using PANi−8 wt‰ graphene complex CE in comparison with 6.40% from pure PANi CE-based DSSC. The high conversion efficiency, facile charge-transfer in combination with simple preparation, relatively low cost, and scalability demonstrates the potential use of PANi−graphene complexes in robust DSSCs.
    Journal of Power Sources 01/2014; 256:8–13. · 5.26 Impact Factor
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    ABSTRACT: In order to make an exact prediction for the density of the lead-acid battery electrolyte, this paper proposes a method by using a genetic algorithm to optimise the support vector regression. In this AGA-SVR model, a kind of adaptive genetic algorithm is exploited to choose the model parameters of support vector regression for obtaining better prediction performance. The proposed predicting model is applied to the density predicting for lead-acid battery. The experimental results indicate that both GA and AGA have good efficiency on parameter optimisation. Furthermore, the AGA-SVR model provides a superior prediction performance than the other three models including SPSO-SVR model, IPSO-SVR model and GA-SVR model. Therefore, the AGA method could be considered as an effective alternative method for predicting electrolyte density.
    Int. J. of Modelling. 01/2014; 21(1):29 - 37.
  • Electrochimica Acta. 01/2014; 125:510–515.
  • Journal of the American College of Surgeons 01/2014; · 4.50 Impact Factor
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    ABSTRACT: Layer-by-layer self-assembly is a versatile technique for the construction of well-defined nanoarchitectures with outstanding electrical and photoelectric performances. The revelation of a potential charge-transfer mechanism of extraordinary electrical and photoelectric behaviors is profound in the design of modern electrical and photoelectrical devices. With the aim of revealing the potential charge-transfer mechanism in conducting multilayer films, in this study, we fabricated [poly(styrene sulfonate)/polyaniline]n [(PSS/PANi)n] multilayers with peculiar electrical and photoelectrical features. The fantastic increments in sheet conductivity and photoelectric response were believed to be the percolation reflection of accumulative electrons tunneled across the insulating PSS from the bottom to the top of the conjugated structure of PANi. These profound phenomena, along with simple fabrication and a well-defined architecture, promise that the conducting multilayers will be good candidates for electronic and optoelectronic nanodevices. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40258.
    Journal of Applied Polymer Science 12/2013; · 1.40 Impact Factor
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    ABSTRACT: Aims: Th17 cells contributed to myocardial inflammatory injury in acute viral myocarditis (AVMC), and the migration of these cells were mainly mediated by CCL20-secreting inflammatory cells. However, whether and how the resident cells such as cardiomyocytes and cardiac fibroblasts could mediate Th17 cell migration into the heart remains unclear in AVMC. Methods: The effect of CCL20 on the dynamic alterations of intracardiac Th17 cells and disease severity were investigated through the neutralization of CCL20 in AVMC mice. The key cells releasing CCL20 in the heart and the effects of CCL20-secreting cells on Th17 cell arrest, migration and differentiation were detected in vitro. Results : Neutralization of CCL20 efficiently repressed the myocardial inflammation along with the reduction of Th17 cell infiltrations in the course of AVMC. In vitro, after stimulations of TNF-α, IL-1β and IL-17, cardiac fibroblasts rather than cardiomyocytes could be dominantly induced for CCL20 production. CCL20-secreting cardiac fibroblasts boosted Th17 cell arrest on endothelium, and induce Th17 cell migration. However, CCL20 produced by cardiac fibroblasts had no effect on Th17 cell differentiation and IL-17 production. Conclusions: It firstly suggested that cardiac fibroblasts could recruit Th17 cells infiltration into myocardium by secreting CCL20 in AVMC. © 2013 S. Karger AG, Basel.
    Cellular Physiology and Biochemistry 11/2013; 32(5):1437-1450. · 3.42 Impact Factor
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    ABSTRACT: Although IL-17 is considered to promote B cell differentiation into antibody-secreting plasma cells in some autoimmune diseases, its mechanism remains unclear. Recent studies revealed that autophagy, a lysosome-mediated catabolic process for providing nutrients under starvation, could regulate plasma cell homeostasis, so this study aimed to explore whether and how autophagy participates in IL-17-mediated plasma cell differentiation by MyHC-α-induced experimental autoimmune myocarditis (EAM) mouse model. It showed that IL-17 could not only induce B cell autophagy, but also facilitate the myocarditis severity, serum anti-MyHC-α autoantibody production and splenic CD38(+) CD138(+) B cell percentages, while the autophagy inhibitor 3-methyladenine attenuated these effects. Furthermore, serum anti-MyHC-α IgG autoantibody productions and CD38(+) CD138(+) B cell percentages were positively correlated with B cell autophagy levels respectively. In vitro, we further revealed that IL-17 could directly promote B cell autophagy, which boosted Blimp-1 expressions and CD38(+) CD138(+) B cell percentages. Moreover, elevated autophagy mediated by IL-17 enhanced ubiquitin-proteasome system activity and B cell anti-apoptotic ability by Beclin-1 and p62 through Erk1/2 phosphorylation, and these changes brought by IL-17 could be also inhibited with 3-methyladenine. Therefore, we concluded that autophagy contributed to IL-17-mediated plasma cell differentiation by regulating Blimp-1 expression and Beclin-1/p62 associated B cell apoptosis in EAM.
    International immunopharmacology 11/2013; · 2.21 Impact Factor
  • Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] 11/2013; 41(11):949.
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    ABSTRACT: The autoantibodies against angiotensin AT1 receptors (AT1-AAs) in patients with essential hypertension exhibited an agonistic action like angiotensin II and maintained high blood pressure (BP). Angiotensin II receptor gene (AGTR1) polymorphisms were associated with BP response to RAS inhibition in the hypertensive population. Furthermore, the BP response to AT1 receptor blockers varied significantly among individuals with hypertension. We hypothesized that the polymorphisms of the AGTR1 and AT1-AAs might affect antihypertensive response to AT1 receptor blockers based in patients with primary hypertension. Patients who received a candesartan-based regimen came from the SOT-AT1 study (Study of Optimal Treatment in Hypertensive Patients with Anti-AT1-Receptor Autoantibodies). The established enzyme-labeled immunosorbent assay was used to detect AT1-AAs in the sera of the patients. Genotype 3 single nucleotide polymorphisms in AGTR1 gene was used by DNA sequencing. The correlations among AT1-AAs, AGTR1 gene polymorphisms or haplotypes, and the antihypertensive effect candesartan-based were analyzed using SPSS. The percentage of systolic BP reduction that was candesartan-based was greater in AT1-AA positive groups than in AT1-AA negative ones (21 ± 8 vs. 18 ± 9; P = .001). Meanwhile, systolic BP reduction that was candesartan-based was more significant in the group of rs5186 AC genotypes than AA homozygotes after adjusting for other confounding factors (37.55 ± 13.7 vs. 32.47 ± 17.27 mm Hg; adjusted P = .028). Furthermore, haplotypes (GCC) and (AAC) had impacts on the antihypertensive effect of candesartan therapy. The AT1-AAs, AGTR1 gene polymorphisms and haplotypes solely or jointly have influences on candesartan-based antihypertensive response in patients with primary hypertension.
    Journal of the American Society of Hypertension (JASH) 10/2013;
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    ABSTRACT: Integrated resection of the pancreatic head is the most difficult step in radical pancreaticoduodenectomy (RPD) in patients with the portal vein (PV) and superior mesenteric vein (SMV) invasion or oppression by the tumor. This study introduced a new idea and skill named the "total arterial devascularization first" (TADF) technique and its applications in RPD. Three arterial blood supplies of pancreatic head were obstructed before dissection of veins. The critical steps included exposure of the anterior surface of the abdominal aorta (AA) by completely transecting neural and connective tissue between superior mesenteric artery (SMA) and pancreatic mesounsinate, and transection of the mesounsinate from the origin of SMA to the root of the celiac trunk. From January 2012 through May 2013, a total of 58 patients with PV/SMV invasion or oppression underwent RPD using this technique. The median operative time was 5.1 h (ranging 4.5-8.1 h). The median intraoperative blood loss was 450 mL (ranging 200-900 mL). No intraoperative and postoperative bleeding of pancreatic head region occurred. Among the 58 patients, 21 were subjected to vessel lateral wall angiectomy or angiorrhaphy, and 10 to angiectomy and end-to-end anastomosis. The incidence of postoperative bleeding, postoperative pancreatic fistula and biliary fistula was 5.2%, 6.8%, and 1.7%, respectively. No patients died 3 months after operation. The TADF technique is a new method for intricate RPD and could improve the security of surgery and reduce intraoperative bleeding, which is expected to become standardized surgical approach for RPD.
    Journal of Huazhong University of Science and Technology 10/2013; 33(5):687-91. · 0.58 Impact Factor
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    ABSTRACT: To explore the improvement of typing and reasonable surgical treatment for pancreatic ductal stone (PDS). Totally 89 patients with pancreatic ductul stone treated underwent surgeries from January 2000 to December 2012 were involved into this study. There were 57 male and 32 female patients, the average age was (52 ± 23) years. According to the magnetic resonance cholangiopancreatography imaging and finding during surgery, pancreatolithiasis was classified into three types: type I, the stones were located in the main pancreatic duct; type II, the stones were located both in main and branch pancreatic duct; type III, the stones were diffusely scattered in the branch pancreatic duct; the position of PDS within pancreatic parenchyma were subtitled. In this group, 43 type I PDS were extracted with endoscopic papillotomy or endoscopic pancreatic sphincterotomy, or pancreatolithotomy plus pancreato-jejunal lateral anastomosis with wide anastomotic stoma; 39 type II cases were treated by pancreatolithotomy plus pancreato-jejunal lateral anastomosis or/and resection of pancreatic section; 7 type III PDS were managed with resection of pancreatic section. All surgeries were performed successfully. Among complications, 6 cases (6.7%) were pancreatic leakage which recovered after systematic non-surgical treatment, 2 cases (2.2%) were anastomotic bleeding which led to 1 death, 6 cases (6.7%) were residual pancreatolithiasis in branch pancreatic duct type. Seventy-eight patients were followed up for 6 to 131 months, 57 cases were still alive so far. Five cases were intermittent abdominal pain, 7 cases were diabetes resulted from 2 subtotal pancreatectomy and 5 distal pancreatectomy, 5 cases occurred pancreatolithiasis recurrence and 3 underwent secondary surgeries. The basis of this modified typing of pancreatolithiasis is the position of stone in pancreatic duct rather than pancreas parenchyma. It is more important and valuable for surgical principle of taking stones out completely and maintaining pancreatic function.
    Zhonghua wai ke za zhi [Chinese journal of surgery] 08/2013; 51(8):688-690.
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    ABSTRACT: BACKGROUND: Postoperative pancreatic fistula (POPF) represents the most important cause of morbidity after pancreaticoduodenectomy (PD) and contributes to prolonged hospitalization and increased mortality rates. This study presents a new technique, which involves end-to-end pancreaticojejunostomy with transpancreatic interlocking mattress sutures, and evaluates its safety and reliability. METHODS: From January 2011 to May 2012, 79 patients with periampullary malignancies underwent PD by using this modified technique, and the morbidity and mortality rates were calculated. RESULTS: In this study, all cases recovered well from PD. Four transpancreatic interlocking mattress sutures were performed in 79 patients. The median duration of surgery was 3.9 hr (range 3.1-6.8), and the median time to perform pancreaticojejunostomy was 15.3 min (range 9-24). Overall, morbidity occurred in 16 patients (22.3%), and the causes included upper gastrointestinal bleeding (n = 2), biliary fistula (n = 1), pulmonary infection (n = 1), delayed gastric emptying (n = 1), abdominal abscess (n = 1, caused by PF), wound infection (n = 3), arrhythmia or myocardial infarction (n = 3), urinary tract infection (n = 2), and POPF (n = 2, 2.53%). One patient had grade A POPF, one had grade B POPF, and none of them had grade C POPF. No death occurred during surgery. CONCLUSION: The end-to-end pancreaticojejunostomy with transpancreatic interlocking mattress sutures is a simple, rapid, safe, and reliable technique with low POPF rate and low delayed massive hemorrhage rate. J. Surg. Oncol. 2013 9999:XX-XX. © 2013 Wiley Periodicals, Inc.
    Journal of Surgical Oncology 05/2013; · 2.64 Impact Factor

Publication Stats

669 Citations
240.34 Total Impact Points

Institutions

  • 1970–2014
    • Huazhong University of Science and Technology
      • • Department of Biliary-Pancreatic Surgery
      • • Department of Cardiology
      • • Department of Control Science and Engineering
      Wu-han-shih, Hubei, China
  • 2006–2013
    • Wuhan Union Hospital
      Wu-han-shih, Hubei, China
  • 2007–2010
    • Tongji Hospital
      Wu-han-shih, Hubei, China
  • 2003
    • Korean Institute of Ocean Science and Technology
      Anzan, Gyeonggi Province, South Korea