Manel Ben Mansour

University of Saskatchewan, Saskatoon, Saskatchewan, Canada

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Publications (2)1.55 Total impact

  • Vladimir Vujanovic, Manel Ben Mansour
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    ABSTRACT: Twelve randomly-selected isolates of Fusarium graminearum that produce 3-acetyl-deoxynivalenol (3-ADON) or 15-acetyl-deoxynivalenol (15-ADON) were screened by thin-layer chromatography (TLC) for their ability to produce ADON and zearalenone (ZEA) mycotoxins when grown on water agar containing different concentrations of sucrose. The results showed the ability of the F. graminearum 3-ADON chemotype population to produce DON and ZEA at a lower concentration range of sucrose (5-7%) compared with the 15-ADON chemotype (30-40%). The former distinction allows for sucrose-water agar to be employed as a rapid and simple differential medium, where two separate sucrose-gradient concentrations discriminate 3-ADON from 15-ADON populations. In the light of the shift in sugar concentrations occurring during the process of grain formation and maturation, the difference in mycotoxin production between the two populations is discussed with respect to predicting Fusarium head blight (FHB) epidemiology and accumulation of DON and ZEA.
    Mycotoxin Research 11/2011; 27(4):295-301.
  • Manel Ben Mansour, Yit Kheng Goh, Vladimir Vujanovic
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    ABSTRACT: Sucrose-water medium induced a high rate of macroconidia formation in Fusarium graminearum 3- and 15-acetyldeoxynivalenol (ADON) chemotypes. The F. graminearum isolates tested produced uniform macroconidia in large quantities and within a shorter incubation period in sucrose-water medium compared to other assessed media. At the same time, high rates of mycotoxin accumulation (trichothecenes: DON, 3-ADON, 15-ADON, and zearalenone: ZEA) in macroconidia were detected using high performance liquid chromatography. The proposed “saccharose-water” method represents a valuable alternative for conidia production in mycotoxigenic F. graminearum isolates, having advantages over the potentially mutagenic UV light, and potato-dextrose agar, which can degenerate macroconidia, and over expensive chemical compounds or natural substances for which the preparation protocols and handling procedures are relatively complex or time-consuming.
    Annals of Microbiology 01/2011; · 1.55 Impact Factor

Publication Stats

1 Citation
1.55 Total Impact Points

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Institutions

  • 2011
    • University of Saskatchewan
      • Department of Food and Bioproduct Sciences
      Saskatoon, Saskatchewan, Canada