[Show abstract][Hide abstract] ABSTRACT: Objective
The purpose of the research was to investigate the presence of Listeria, Salmonella and Klebsiella on samples
of enteral diets and utensils, surfaces and equipments involved in food preparation in a hospital food service.
Fifty samples collected from utensils, surfaces and equipment used for food preparation in a hospital food
service, and four samples collected from enteral diets were tested for bacteria of genera Listeria, Salmonella
and Klebsiella. Typical colonies of bacteria of the genus Listeria from enteral diet were isolated in Oxford agar
and contamination by L. monocytogenes was confirmed by immunoanalysis.
L. monocytogenes, S. rissen and Klebsiella were isolated from enteral diet. For their relevance as agents of
hospital infections, bacteria of the genus Klebsiella were evaluated. K. pneumoniae were found in equipment
and utensil, and K. oxytoca were found in environment, equipment and enteral diet samples. L. monocytogenes
showed resistance to cefoxitin and all Klebsiella were resistant to amoxacillin and ampicillin. S. rissen showed
susceptibility to all 13 antibiotics tested.
The study showed that 11% of the analyzed samples were contaminated with, at least, one of the investigated
pathogens. Such results reiterate the need for awareness and knowledge of effective hygienic procedures in
the hospital food manipulation areas, in order to ensure patients’ safety