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ABSTRACT: A strictly aerobic, Gram-positive, motile and spore-forming bacterium, strain 3nP4(T), was isolated from the Puge hot spring located in the southwestern geothermal area of China. Strain 3nP4(T) grew within the ranges of 38-66 °C (optimum 57-60 °C), pH 6.0 to 9.3 (optimum 7.0-7.5) and 0-4% (w/v) NaCl (optimum 0-0.5%). Phylogenetic analysis of 16S rRNA gene sequences, as well as the levels of DNA-DNA relatedness indicated that the new isolate represents a novel species of the genus Anoxybacillus with closest relation to Anoxybacillus voinovskiensis DSM 12111(T). Strain 3nP4(T) had DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PE (phosphatidylethanolamine), and one phospholipid (PL1) as major polar lipids and had iso-branched C15:0 and C17:0 as major fatty acids, which are both typical chemotaxonomic characteristics of the genus Anoxybacillus. The DNA G+C content of strain 3nP4(T) was 39.2±0.95 mol% (HPLC). Extremely reliant on vitamin mixture or yeast extract for growth is the most distinctive characteristic of the new isolate. Based on the polyphasic taxonomic characteristics, we have concluded that strain 3nP4(T) (= CGMCC 1.8979(T) = JCM 16594(T)) represents a novel species of the genus Anoxybacillus with suggested name Anoxybacillus vitaminiphilus sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2013; · 2.11 Impact Factor
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ABSTRACT: A thermotolerant, extremely halophilic archaeon BC12-B1(T) was isolated from a salt mine in Baicheng county, Xinjiang province, China. Colonies were off-white-grey and the cells stained Gram-negative, were motile and irregularly long rod-shaped (variation in both width and length), with abundant gas vesicles. The strain was able to grow at 20-55°C (optimum 48°C), pH 6.0-8.0 (optimum 7.0-7.3), 1.8-6.0 M NaCl (optimum 3.0-3.5 M) and 0.02-2.2 M Mg2+ (optimum 0.1-0.2 M). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8% (w/v). Phylogenetic analysis based on the 16S rRNA gene sequences showed strain BC12-B1(T) was most related to Halopelagius inordinatus RO5-2(T) (98.5%) with less than 95% sequence similarity to other described species. The genomic DNA G+C content was 64.0 mol%. The value of DNA-DNA hybridization between strains BC12-B1(T) and Hpl. inordinatus RO5-2(T) was 43.6%. The major polar lipids of strain BC12-B1(T) were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, four glycolipids and an unknown lipid. Based on phenotypic, chemotaxonomic and genotypic characteristics, strain BC12-B1(T) is suggested to represent a novel species of the genus Halopelagius, for which the name Halopelagius longus sp. nov. is proposed. The type strain is BC12-B1(T) (= CGMCC 1.12397T=JCM 18758T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2013; · 2.11 Impact Factor
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ABSTRACT: A novel extremely halophilic archaeon, KCY07-B2(T), was isolated from a salt mine in Kuche county, Xinjiang province, China. Colonies were cream-pigmented and cells were pleomorphic rod-shaped. Strain KCY07-B2(T) was able to grow at 25-50 °C (optimum 37-45 °C) and pH 6.0-8.0 (optimum 7.0). The strain required at least 1.9 M NaCl but for growth MgCl2 was not required. Cells lysed in distilled water. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both C20C20 and C20C25 glycerol diethers together with four glyolipids. The bis-sulfated glycolipid S2-DGD-1 was present. The DNA G+C content was 62.5 mol%. Analysis of the 16S rRNA gene sequence revealed that strain KCY07-B2(T) was closely related to Halopiger xanaduensis SH-6(T) and Halopiger aswanensis 56(T) (95.8% and 95.5% similarity, respectively). On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KCY07-B2(T) is considered to represent a novel species in the genus Halopiger, for which the name Halopiger salifodinae sp. nov. is proposed. The type strain is KCY07-B2(T) (=JCM 18547(T) =CGMCC 1.12284(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2013; · 2.11 Impact Factor
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ABSTRACT: A Gram-negative, rod-shaped bacterium with appendages, designated Ar-22(T), was isolated from a seawater sample collected from the western part of the Prydz Bay, near Cape Darnley, Antarctica. Strain Ar-22(T) grew optimally at 35 (o)C, at pH 7.5 and in the presence of 1-3 % (w/v) NaCl. The isolate was positive for casein, gelatin and Tween 20 decomposition and negative for H2S production as well as indole formation. Chemotaxonomic analysis showed MK-6 as major isoprenoid quinone and phosphatidylethanolamine as major polar lipid. The major fatty acids were iso-C17:0 3OH, iso-C15:1 G, iso-C15:0 and C16:1ω7c/iso-C15:0 2OH. The genomic DNA G + C content was 44.8 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain Ar-22(T) was closely related to members of the genus Muricauda , sharing 94.2-97.3 % sequence similarity with the type strains of Muricauda species and being most closely related to the M. aquimarina. Phylogenetic analysis based on the 16S rRNA gene sequence comparison confirmed that strain Ar-22(T) formed a deep lineage with M. flavescens. Sequence similarity between strain Ar-22(T) and M. ruestringensis DSM 13258(T), the type species of the genus Muricauda, was 96.9 %. Strain Ar-22(T) exhibited mean DNA-DNA relatedness values of 40.1 %, 49.4 % and 25.7 % to M. aquimarina JCM 11811(T), M. flavescens JCM 11812(T) and M. lutimaris KCTC 22173(T), respectively. On the basis of phenotypic and genotypic data, strain Ar-22(T) represents a novel species of the genus Muricauda, for which the name Muricauda antarctica sp. nov. (type strain Ar-22(T) = CGMCC 1.12174(T) = JCM 18450(T)) is proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 03/2013; · 2.11 Impact Factor
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ABSTRACT: An aerobic, Gram-negative, short rod-shaped, non-motile and non-sporulating bacterium, designed strain 8-1bT, was isolated from seaweeds collected from the intertidal zone of Zhoushan sea area, East China Sea. Strain 8-1bT grew at 4-39°C (optimum 28-32°C) and pH 6.0-9.5 (optimum 7.0-8.5), 0.5-8% NaCl (optimum 1-3%) and 0.5-10% sea salts (optimum 2-3%) (w/v). Analysis of 16S rRNA gene sequences revealed that strain 8-1bT was closely related to Aequorivita capsosiphonis A71T (96.7%). The DNA G+C content was 36.6 mol%. Compared to the reference strains, cells of strain 8-1bT showed positive results for H2S production and utilization of D-mannose, D,L-lactic acid, L-asparagine and glycyl-L-aspartic acid. The major fatty acids of strain 8-1bT were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G and iso-C17:1ω9c. The main respiratory quinone was menaquinone 6. The polar lipids of strain 8-1bT consisted of one phosphatidylethanolamine (PE), three uncharacterized aminolipids (AL1~3), four uncharacterized glycolipids (GL1~4) and five uncharacterized lipids (L1~5). Based on the phenotypic and genotypic characterization, strain 8-1bT (=CGMCC 1.11023T =JCM 18497T) is considered to represent a novel species of the genus Aequorivita, for which the name Aequorivita viscosa sp. nov. is proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2013; · 2.11 Impact Factor
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ABSTRACT: A novel strain, named S4(T), was obtained from an industrial wastewater in Xiaoshan, Zhejiang Province, China. Cells were Gram-negative, neutrophilic and non-spore-forming, and moved by polar flagellum. Normal cells were 0.8-0.9 × 1.3-1.9 μm and the cells elongated when cultivated at higher temperature. Strain S4(T) grew at 15-50 °C (optimum at 48 °C), pH 5.5-8.5 (optimum 7.0-7.5), and 0-2% (optimum 0.5% w/v) NaCl. Substrates utilized as sole carbon sources were alanine, asparagine, glutamate, citrate, malonate, salicylate and succinate. Ubiquinone-8 was the predominant respiratory quinone. C(16:0), summed feature 3 (C(16:1) ω7c and/or iso-C(15:0) 2-OH), C(17:0) cyclo and C(18:1) ω7c were the major cellular fatty acids. Major 3-OH fatty acid was C(10:0) 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminoglycolipids.The genomic DNA G+C content was 68.8 mol%. Based on 16S rRNA gene sequences alignment, the close genera were Comamonas (94.6-95.6%), Giesbergeria (94.9-95.6%), Acidovorax (94.8-95.4%), Brachymonas (95.2%), Macromonas (95.1%). Phylogenetic analyses showed the closest relatives of strain S4(T) to be genera Macromonas. Based on phenotypic and phylogenetic characteristics, we suggest that strain S4(T) represent a novel species of a new genus of the family Comamonadaceae, for which the name Extensimonas vulgaris gen. nov., sp. nov. is proposed. The type strain is S4(T) (= CGMCC 1.10977(T) =JCM 17803(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 10/2012; · 2.11 Impact Factor
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ABSTRACT: A novel anaerobic, heterotrophic bacterium, designated strain Zn2(T) was isolated from the wastewater of a paper mill in Zhejiang, China. Cells were Gram-positive, rod-shaped, 0.5-0.8 µm in width and 2-4 µm in length, and were motile by lateral flagellum. The temperature and pH ranges for growth were 10-55 °C and 6.0-9.5, respectively. Optimal growth occurred at 35 °C and pH 7.3-7.5. The strain did not require NaCl for growth but its inclusion in the medium (optimum concentration of 6 %) improved growth. Substrates utilized as sole carbon sources were peptone, tryptone, casamino acids, D-xylose, salicin, glycerol, formate, acetate and propionate. The main product of carbohydrate fermentation was acetate, formate, propionate and lactate. Elemental sulfur, sodium thiosulfate and Fe(III) were used as electron acceptors but sodium sulfate, sodium sulfite, sodium nitrate, nitrite and Mn(IV) were not. Growth was inhibited by the addition of 10 µg/ml of ampicillin, penicillin, tetracycline or chloramphenicol. Iso-C(15:0), C(14:0), C(16:0), C(16:1) cis-9 and C(18:1) cis-9 were major fatty acids. Strain Zn2(T) did not have any menaquinone or ubiquinone. The main polar lipids of strain Zn2(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine, two unknown phospholipids and four unknown glycolipids. The genomic DNA G+C content was 37 mol% as determined by HPLC. 16S rRNA gene sequence analysis revealed that strain Zn2(T) was a member of family Clostridiaceae, and was most closely related to Geosporobacter subterraneus, Thermotalea metallivorans and Caminicella sporogenes, showing 91.2%, 90.3% and 91.1% sequence similarity respectively. On the basis of its phenotypic and genotypic properties, strain Zn2(T) was suggested to represent a novel species of a genus, for which the name Salimesophilobacter vulgaris gen. nov., sp. nov. is proposed. The type strain is Zn2(T) (= DSM 24770(T) = JCM 17796(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 07/2012; · 2.11 Impact Factor
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ABSTRACT: Halophilic archaeon AJ6 was isolated and purified from the Altun Mountain National Nature Reserve of the Xinjiang Uygur Autonomous
Region. Strain AJ6 is a Gram-negative rod whose size is 0.2–0.6 by 1.6–4.2 µm, wherein a few cells are globular. The optimum
salt concentration for its growth is 20% NaCl and 0.6% Mg2+, and the optimum pH is 6.0–7.0. Morphological, physiological, and biochemical characteristics of strain AJ6 were observed.
The 16S rRNA encoding gene (16S rDNA) sequence of strain AJ6 was amplified by PCR, and its nucleotide sequence was determined
subsequently. “Clustalw” and “PHYLIP” software bags were used to analyze the 16S rDNA sequence; the homology was compared,
and then the phylogenetic tree was established. The results indicate that strain AJ6 is a novel species of the genus Natrinema. The GenBank accession number of the 16S rDNA sequences of strain AJ6 is AY277584.
Frontiers of Biology in China 04/2012; 1(1):29-34.
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ABSTRACT: The gene coding for insulin-like growth factor-binding protein 3 (IGFBP3) is important for regulation of growth, development and metabolism in mammals. The present investigation was conducted to study nucleotide polymorphism of the IGFBP3 in gayal (Bos frontalis) and to compare the variations with those which occur in other ruminants. A fragment of 645 base pairs of the IGFBP3 covering a part of exon 2, the complete intron 2 and exon 3 and a part of intron 3 was amplified, sequenced (n=46) and digested (n=79) with HaeIII restriction enzyme from 125 collected gayal samples. Nine single nucleotide polymorphisms (SNPs) [C14T, A122C, C137T, G144C, C155T, G213A, C279A, G334A and G460A] were identified and located in intron 2, revealing high genetic variability. The alignment of nucleotide sequences was found to be very similar to those for other bovid species. Sequencing and HaeIII digestion showed that frequency of alleles C and A [consisting of fragments of sizes 56, 64, 228, 264, 282, 298 and 497 bp (CC genotype)] was 0.96 and 0.04 for the SNP C279A. Moreover, the genotype frequency of the SNP C279A in gayal was compared with that in other ruminants and it appears that this polymorphism may be associated with low fat content and rapid growth in this rare species.
Gene 04/2012; 497(1):98-102. · 2.34 Impact Factor
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ABSTRACT: A chitosanase gene, csn, was cloned from Penicillium sp. D-1 by inverse PCR. The cDNA sequence analysis revealed that csn had no intron. The deduced CSN protein consists of 250 amino acids including a 20-amino acid signal peptide, and shared 83.6% identity with the family 75 chitosanase from Talaromyces stipitatus (B8M2R4). The mature protein was overexpressed in Escherichia coli and purified with the affinity chromatography of Ni2+-NTA. The novel recombinant chitosanase showed maximal catalytic activity at pH 4.0 and 48°C. Moreover, the activity of CSN was stable over a broad pH range of 3.0-8.0, and the enzymatic activity was significantly enhanced by Mg2+ and Mn2+. The CSN could effectively hydrolyze colloidal chitosan and chitosan, while could not hydrolyze chitin and carboxymethylcellulose (CMC). Due to the particular acidophily, CSN has the potential application in the recycling of chitosan wastes.The GenBank/EMBL/DDBJ accession numbers for the 18S rRNA gene and chitosanase gene of strain D-1 are JF950269 and JF950270, respectively.
AMB Express. 02/2012; 2(1):13.
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ABSTRACT: A novel mesophilic, strictly anaerobic bacterium, strain BM(T), was isolated from food industry wastewater. The cells were motile, non-spore-forming rod and stained Gram-negative. Growth of strain BMT was observed at 16-44 °C (optimum 37 °C) and pH 6.0-9.0 (optimum 7.5). The NaCl concentration range for growth was 0-8% (optimum 1.5%, w/v). Strain BM(T) was chemo-organotrophic using a few sugars and amino acids as sole carbon and energy sources. The fermentation products from peptone-yeast broth were propionate, formate, acetate, ethanol and isovalerate. Indole, NH(3) and H(2)S were produced from peptone. No respiratory quinones can be detected. The major fatty acids were iso-C(15:0) (39.3%), iso-C(15:0) DMA (10.1%), anteiso-C(15:0) (7.6%), C(14:0) (6.1%), and C(16:0) (5.6%). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, aminoglycolipids, glycolipids and phospholipids. The DNA G+C content was 28.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain BM(T) was related with different genus belonging to the family Clostridiaceae, and its closest relatives were Sporosalibacterium faouarense JCM 15487(T) (94.3% 16S rRNA gene sequence similarity), Proteiniborus ethanoligenes JCM 14574(T) (92.1%) and Clostridiisalibacter paucivorans JCM 14354(T) (92.0%). Due to its phenotypic and genotypic characteristics, isolate BM(T) is proposed as a novel species of a new genus, Brassicibacter mesophilus gen. nov., sp. nov. The type strain is BM(T) (=JCM 16868(T)=DSM 24659(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2012; · 2.11 Impact Factor
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Ying-Yi Huo,
Hong Cheng,
Xi-Fang Han,
Xia-Wei Jiang,
Cong Sun,
Xin-Qi Zhang,
Xu-Fen Zhu,
Yong-Feng Liu,
Peng-Fei Li,
Pei-Xiang Ni, Min Wu
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ABSTRACT: Pelagibacterium halotolerans B2(T) is a marine halotolerant bacterium that was isolated from a seawater sample collected from the East China Sea. Here, we present the complete genome sequence of the type strain P. halotolerans B2(T), which consists of one chromosome (3,944,837 bp; 61.4% G+C content) and one plasmid (4,050 bp; 56.1% G+C content). This is the first complete genome of a member of the Pelagibacterium genus.
Journal of bacteriology 01/2012; 194(1):197-8. · 3.94 Impact Factor
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ABSTRACT: A bacterial strain named NS31-3(T) was isolated from the wastewater of a paper mill. The isolate was obligately anaerobic, non-pigmented, non-motile, gram-negative and short rod-shaped with 2.0-2.8 µm in width, 2.3-4.7 µm in length. The strain was able to grow on media containing 20% bile salts. API 20A tests showed that acid was produced from glucose, lactose, sucrose, maltose, D-xylose, L-arabinose, cellobiose, D-mannose, D-melizitose,D-raffinose, D-trehalose, D-mannitol, salicin and D-sorbitol. The main fermentation products from PYG broth were lactic acid, propionic acid, formic acid and acetic acid. Chemotaxonomic analysis showed that anteiso-C(15:0), C(15:0) and iso-C(17:0 )3-OH were the major fatty acids, and the predominant respiratory quinine was MK-9 and MK-10. Phylogenetic analysis based on 16S rRNA gene sequence showed that the strain NS31-3(T) was related to the members of genus Parabacteroides with 91.2-93.2% sequence similarity and with cloest affinity to Parabacteroides merdae. The DNA G+C content of the genomic DNA was 37.2 mol%. Based on the polyphasic taxonomic characterization, strain NS31-3(T) should represent a novel species of the genus Parabacteroides, for which the name Parabacteroides chartae sp. nov. is proposed. The type strain is NS31-3(T) (= JCM 17797(T)= DSM 24967(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 12/2011; · 2.11 Impact Factor
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ABSTRACT: A novel Gram-negative, non-spore-forming, strictly anaerobic, heterotrophic bacterium, strain TY(T), was isolated from salty pickle wastewater. Cells were rod-shaped with comb-like flagella, slightly curved and very variable in length. Optimal growth occurred at 28 °C and pH 6.5. Cells were resistant to up to 50 g NaCl l(-1). Strain TY(T) produced acid from glycerol, sucrose, glucose, fructose and mannitol. The main fermentation products from glucose were acetic and propionic acids. Tests for acid phosphatase and naphthol-AS-BI-phosphohydrolase activities were positive. The major fatty acids were C(14 : 0) DMA (18.7 %), C(15 : 0) (15.4 %), anteiso-C(18 : 1) (15.2 %), C(11 : 0) (13.3 %) and summed feature 5 (C(17 : 1)ω7c and/or C(17 : 2)) (11.0 %). The DNA G+C content was 35.9 mol%. 16S rRNA gene sequence-based phylogenetic analysis indicated that strain TY(T) represented a novel species of the genus Pectinatus (sequence similarity to other members of the genus ranged from 93.2 to 94.8 %). Based on its phenotypic, genotypic and phylogenetic characteristics, strain TY(T) is proposed to represent a novel species, named Pectinatus brassicae sp. nov. (type strain TY(T) = JCM 17499(T) = DSM 24661(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2011; 62(Pt 9):2145-9. · 2.11 Impact Factor
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ABSTRACT: Cobalt-rich crusts are important metallic mineral resources with great economic potential, usually distributed on seamounts located in the Pacific Ocean. Microorganisms are believed to play a role in the formation of crusts as well as in metal cycling. To explore the microbial diversity related to cobalt-rich crusts, 16S ribosomal RNA gene clone libraries were constructed from three consecutive sediment layers. In total, 417 bacterial clones were obtained from three bacterial clone libraries, representing 17 distinct phylogenetic groups. Proteobacteria dominated in the bacterial communities, followed by Acidobacteria and Planctomycetes. Compared with high bacterial diversity, archaea showed a remarkably low diversity, with all 137 clones belonging to marine archaeal group I except one novel euryarchaeotal clone. The microbial communities were potentially involved in sulfur, nitrogen and metal cycling in the area of cobalt-rich crusts. Sulfur oxidation and metal oxidation were potentially major sources of energy for this ecosystem. This is the first reported investigation of microbial diversity in sediments associated with cobalt-rich crusts, and it casts fresh light on the microbial ecology of these important ecosystems.
FEMS Microbiology Ecology 08/2011; 78(3):565-85. · 3.41 Impact Factor
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ABSTRACT: A new agarase, AgaA(CN41), cloned from Vibrio sp. strain CN41, consists of 990 amino acids, with only 49% amino acid sequence identity with known β-agarases. AgaA(CN41) belongs to the GH50 (glycoside hydrolase 50) family but yields neoagarotetraose as the end product. AgaA(CN41) was expressed and characterized.
Applied and environmental microbiology 08/2011; 77(19):7077-9. · 3.69 Impact Factor
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ABSTRACT: Two Gram-negative, motile, aerobic bacterial strains, designated B2(T) and 1_C16_27(T), were respectively isolated from a seawater sample collected from the East China Sea and a semi-coke sample from north-eastern Estonia. Their genetic, phenotypic and chemotaxonomic properties were studied. The isolates were short rods with polar flagella and were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The major fatty acids were nonadecanoic (C(19 : 0) cyclo), octadecanoic (C(18 : 0) and C(18 : 0) 3-OH), octadecenoic (C(18 : 1)) and hexadecanoic (C(16 : 0)) acids. The G+C content of the genomic DNA was 58.1-59.3 mol%. 16S rRNA gene sequence analysis revealed that the two isolates represent a distinct lineage within the family Hyphomicrobiaceae. The phylogenetically closest relatives were Cucumibacter (92.7-93.7 % 16S rRNA gene sequence similarity), Devosia (92.9-94.4 %) and Zhangella (91.7-92.1 %). Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strains B2(T) and 1_C16_27(T) could be differentiated from each other and from members of the genera Cucumibacter, Devosia and Zhangella. Therefore, it is proposed that strains B2(T) and 1_C16_27(T) represent two novel species in a new genus, for which the names Pelagibacterium halotolerans gen. nov., sp. nov. (the type species; type strain B2(T) = CGMCC 1.7692(T) = JCM 15775(T)) and Pelagibacterium luteolum sp. nov. (type strain 1_C16_27(T) = CGMCC 1.10267(T) = JCM 16552(T) = CELMS EEUT 1C1627(T)) are proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2011; 61(Pt 8):1817-22. · 2.11 Impact Factor
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ABSTRACT: A Gram-reaction-negative, rod-shaped, motile, neutrophilic bacterium, designated strain BH3(T), was isolated from wastewater of a sequential batch reactor treating wastewater taken from a leather plant. The isolate grew in 0-8 % (w/v) NaCl, at pH 6-9 and at 4-45 °C. Chemotaxonomic analysis showed that strain BH3(T) had characteristics typical of members of the genus Sphingomonas, such as the presence of sphingolipids, Q-10 and 2-hydroxymyristic acid and the absence of 3-hydroxy fatty acids. The presence of C(18 : 1)ω7c (39.2 %) and C(16 : 0) (11.2 %) as major fatty acids, C(14 : 0) 2-OH (20.6 %) as the major 2-hydroxy fatty acid and homospermidine as the major polyamine indicated that strain BH3(T) belonged to the genus Sphingomonas sensu stricto. The genomic DNA G+C content of strain BH3(T) was 65.6 mol%. 16S rRNA gene sequence similarities between the isolate and closely related members of the genus Sphingomonas sensu stricto ranged from 92.6 to 97.3 %, the highest sequence similarities being to Sphingomonas melonis DSM 14444(T) (97.3 %) and Sphingomonas aquatilis DSM 15581(T) (97.3 %). Based on its phenotypic characteristics and the results of DNA-DNA hybridization studies and 16S rRNA gene sequence comparisons, strain BH3(T) represents a novel species of the genus Sphingomonas sensu stricto, for which the name Sphingomonas rubra sp. nov. is proposed. The type strain is BH3(T) ( = CGMCC 1.9113(T) = JCM 16230(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 05/2011; 61(Pt 5):1028-32. · 2.11 Impact Factor
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ABSTRACT: Two Gram-negative, aerobic strains, Y215(T) and Y226(T), were isolated from sediment from Yueqing Bay, Zhejiang Province, China. The two novel strains were both positive for oxidase activity, nitrate reduction, and aesculin and casein decomposition, but negative for gelatin and tyrosine decomposition. Catalase activity, and starch and Tween 80 decomposition differed between the two strains. Cells of both novel strains were rod-shaped in young cultures and ovoid in older cultures. Optimum NaCl concentration and pH range for growth of both strains were 2.0-3.0% (w/v) and 7.0-8.0, respectively, whereas the optimum growth temperature for strain Y215(T) (25-30 °C) was lower than that for strain Y226(T) (30-37 °C). The genomic DNA G+C contents of strains Y215(T) and Y226(T) were 54.0 and 56.7 mol%, respectively. The major fatty acids in both isolates were iso-C(15:0) and iso-C(17:1)ω9c, which was also the case in the reference strains apart from Microbulbifer salipaludis, which possessed C(18:1)ω7c as the predominant fatty acid. The predominant isoprenoid quinone was Q-8 and the major polar lipids of both strains were phosphatidylethanolamine, phosphatidylglycerol and an unknown glycolipid. Both strains had highest 16S rRNA gene sequence similarity to members of the genus Microbulbifer. Strain Y215(T) was closely related to the type strains of Microbulbifer maritimus (97.6%) and Microbulbifer donghaiensis (97.5%), whereas strain Y226(T) was closely related to the type strain of M. salipaludis (97.6%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strains Y215(T) and Y226(T) fell into two separate clusters. The DNA-DNA relatedness values of strain Y215(T) with M. maritimus TF-17(T) and M. donghaiensis CN85(T) were 34.1 and 32.8%, respectively, whereas that between strain Y226(T) and M. salipaludis SM-1(T) was 38.0%; these values are significantly lower than the threshold value for the delineation of bacterial species. On the basis of their distinct taxonomic characteristics, the two isolates represent two novel species of the genus Microbulbifer, for which the names Microbulbifer marinus sp. nov. and Microbulbifer yueqingensis sp. nov. are proposed; the type strains are Y215(T) (=CGMCC 1.10657(T)=JCM 17211(T)) and Y226(T) (=CGMCC 1.10658(T)=JCM 17212(T)), respectively.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 3):505-10. · 2.11 Impact Factor
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ABSTRACT: A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1(T), was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1-1.6 µm wide and 2.3-4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10-40 °C and pH 6.0-8.9; optimal growth occurred at 28-32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1% (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9% (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, D-glucose, D-fructose, D-xylose, D-ribose, D-galactose, maltose, L-rhamnose, trehalose, L-valine, DL-alanine plus L-proline and DL-alanine plus L-glycine. The main products of glucose fermentation were ethanol and acetate. iso-C(15:0), iso-C(14:0), C(16:0) and iso-C(13:0) were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Tepidibacter, showing 94.7 and 94.1% similarity to the type strains of Tepidibacter formicigenes and Tepidibacter thalassicus, respectively. The genomic DNA G+C content of strain B1(T) was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1(T) is suggested to represent a novel species of the genus Tepidibacter, for which the name Tepidibacter mesophilus sp. nov. is proposed. The type strain is B1(T) (=CGMCC 1.5148(T) =JCM 16806(T)).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 02/2011; 62(Pt 1):66-70. · 2.11 Impact Factor
