Min Zhang

Jiangsu University, Chenkiang, Jiangsu Sheng, China

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Publications (94)217.41 Total impact

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    ABSTRACT: Backgrounds: Health-care workers' (HCWs) exposure to bodily fluids puts them at risk of hepatitis B virus HBV infection. This study investigated HBV vaccination practices and outcomes in HCWs and assessed postvaccination seroprotection across HCWs in different departments. Methods: A survey of HCWs in a Chinese public general hospital was carried out with a retrospective cohort of 1420 hospital HCWs (458 males and 962 females). HBV vaccination status (10-μg/dose used) was investigated in the cohort from vaccination records from the period of 1988 to 2008. Blood samples were collected and tested for hepatitis B surface antigen (HBsAg) and HBV antibodies (anti-HBs). Results: The overall vaccination (complete course) and HBsAg carrier rates among HCWs were 40.42 % (574/1420) and 6.13 % (87/1420), respectively. Vaccination rates differed by department, with HCWs in internal medicine (39.5 %) and emergency (42.0 %) departments having particularly low rates. The natural infection rate was 7.53 % (107/1420) among HCWs. HCWs in the department of infectious diseases (vaccination rate, 57.8 %) had the highest rate of antibody produced by natural infection (88.2 %). Conclusion: The vaccination rate was a disappointingly low among HCWs in Pearl River Delta Area of China. HCWs working in infectious diseases departments and technicians were at particularly likely to have been infected with HBV. A concerted effort is needed to bring vaccination rates up among Chinese HCWs in Pearl River Delta Area of southern China.
    BMC Infectious Diseases 12/2015; 15(1). DOI:10.1186/s12879-015-1278-0 · 2.61 Impact Factor
  • Wang-Dong Xu · Min Zhang · Yi Zhao · Yi Liu ·
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    ABSTRACT: Systemic lupus erythematosus (SLE) is an autoimmune disease. The friend leukemia insertion site 1 (Fli-1) belongs to the Ets family of transcription factors. Recent findings suggested that expression of Fli-1 was abnormal in SLE patients and lupus mice. In addition, functional analysis indicated that Fli-1 plays a key role in the development of this complex autoimmune disorder. Here, we review the updated evidence indicating the roles of Fli-1 in autoimmune lupus. Hopefully, the information obtained may result in a better understanding of the pathogenesis of the systemic autoimmune disease.
    Inflammation 09/2015; DOI:10.1007/s10753-015-0257-3 · 2.21 Impact Factor
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    ABSTRACT: In this study, the adsorption/desorption characteristics of mulberry anthocyanins (MA) on five types of macroporous resins (XAD-7HP, AB-8, HP-20, D-101 and X-5) were evaluated, XAD-7HP and AB-8 showed higher adsorption/desorption capacities. On the basis of static adsorption test, XAD-7HP and AB-8 resins were selected for kinetics, isotherms and thermodynamics. The adsorption mechanism indicated that the process was better explained by pseudo-first-order kinetics and the Langmuir isotherm model, and the thermodynamics tests showed that the processes were exothermic, spontaneous and thermodynamically feasible. Dynamic tests were performed on a column packed with XAD-7HP and AB-8, and breakthrough volume was reached at 15 and 14 bed volumes of MA solution, respectively. The purity of the fraction by 40% ethanol elution on XAD-7HP reached 93.6%, from which cyanidin-3-glucoside and cyanidin-3-rutinoside were identified by HPLC-ESI-MS/MS. The method could be used to prepare high purity anthocyanins from mulberry fruits as well as other plants. Chemical compounds studies in this article: Cyanidin-3-glucoside (PubChem CID:12303203); Cyanidin-3-rutinoside (PubChem CID:29231); Cyanidin (PubChem CID:128861); Hexose (PubChem CID:169005); Rutinose (PubChem CID:441429); Methanol (PubChem CID:887); Ethanol (PubChem CID:702); Ammonium sulfate (PubChem CID:6097028); Acetic acid (PubChem CID:176); Muriatic acid (PubChem CID:313)
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    ABSTRACT: A series of 6-hydroxyaurone derivatives were synthesized in satisfactory yields and characterized by IR, 1H NMR, 13C NMR, and HRMS or elemental analysis. The structure of compound 3e was further confirmed by X-ray crystal analysis. Bioassay results indicated that some of the target compounds displayed moderate herbicidal activity against the dicotyledonous plant Brassica campestris L. at 100 µg·mL−1, and some compounds also showed significant antiproliferative activity against tumor cell lines Hela, HepG-2, and MCF-7.
    Journal of Heterocyclic Chemistry 07/2015; DOI:10.1002/jhet.2497 · 0.79 Impact Factor
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    ABSTRACT: Artemisinin (ART) is a natural drug with potent anticancer activities related with Fe2+ mediated cleavage of endoperoxide bridge in ART. Herein, we reported that Mn2+ could substitute for Fe2+ to react with ART and generate toxic products, inducing a much higher anticancer efficiency. On this basis, we prepared pH-responsive Fe3O4@MnSiO3-FA nanospheres which can efficiently deliver hydrophobic ART into tumors in mice models. Mn2+ was released in acidic tumor environments and intracellular lysosomes, interacting with ART to kill cancer cells. The ART-loaded nanocarriers could suppress tumor growth more efficiently than free ART, which could be further illustrated by Magnetic resonance imaging (MRI). Histological analysis revealed that the drug delivery system had no obvious effects to the major organs of mice. ART has been reported to have lower toxicity than chemotherapeutics. The ART-loaded nanocarriers are promising to improve the survival of chemotherapy patients, providing a novel way for clinical tumor therapy.
    Nanoscale 06/2015; 7(29). DOI:10.1039/C5NR02402A · 7.39 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the efficacy and safety of entecavir (ETV) combined treatment with adefovir (ADV) on chronic hepatitic B (CHB) patients who failed to respond to nucleotide (acid) analog (NA) treatment. On this basis, the possible factors in the combined treatment of these patients will be analyzed. The safety, biochemical index, and the possible factors that might affect the ETV and ADV combined treatment at different points in time were retrospectively analyzed. The biochemical index included the following: virological response, hepatitis B virus (HBV) DNA decline, primary nonresponse, biochemical response, and the hepatitis B virus E antigen/hepatitis B virus E antibody seroconversion rate. There were 94 CHB patients and compensated liver cirrhosis patients who received ETV plus ADV treatment for over 12 weeks after failure of treatment with NAs. The authors have also investigated 76 CHB patients (80.9%) and 18 hepatitis B cirrhosis patients (19.1%) in this study. The HBV DNA baseline was 4.4 ± 1.4 log10 IU/mL, and the positive rate of HBeAg before salvage treatment was 78.7% (74/94). The sample sizes were 94, 78, 42, 10, 6, and 1 for follow-up of 24, 48, 96, 144, 192, and 240 weeks, respectively. The virological responses (HBV DNA < 2 log10 IU/mL) and biochemical responses were 52.1%, 74.3%, and 90.4% and 63.1%, 61.6%, and 81.1%, respectively, at 24, 48, and 96 weeks, which showed significant differences (P < 0.001 and P < 0.005, respectively). The HBV DNA decline was presented as mean ± SEM, which were 1.53 ± 1.23, 1.75 ± 1.37, 2.07 ± 1.54, and 2.39 ± 1.77 log10 IU/mL at 12, 24, 48, and 96 weeks, respectively. They showed significant differences compared with the baseline (χ = 8.084, P < 0.05). The rate of primary nonresponse was 30.9% (29/94), and the primary treatment failure rates were 26.6% (25/94), 24.4% (19/78), and 4.8% (2/42) at 24, 48, and 96 weeks, respectively. They all have statistical difference (P = 0.011 < 0.05). There were 23 patients who experienced virological breakthrough after the HBV DNA levels were undetectable, whereas after follow-up for 12-24 weeks, the HBV DNA levels were back to undetectable again. ETV plus ADV treatment is an efficient and safe treatment for CHB and compensated liver cirrhosis patients who experienced NA treatment failure. The high quantity of baseline HBV DNA level is a risk factor for poor efficacy of salvage treatment.
    American journal of therapeutics 04/2015; Publish Ahead of Print. DOI:10.1097/MJT.0000000000000262 · 1.13 Impact Factor
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    ABSTRACT: Human growth hormone (hGH) plays critical roles in pubertal mammary gland growth, development and sexual maturation. Accumulated studies have reported that autocrine/paracrine hGH is an orthotopically expressed oncoprotein that promotes normal mammary epithelial cell oncogenic transformation. Autocrine/paracrine hGH has also been reported to promote mammary epithelial cell epithelial-mesenchymal transition (EMT) and invasion. However, the underlying mechanism remains largely obscure. miRNAs are reported to involved in regulation of multiple cellular functions of cancer. To determine whether autocrine/paracrine hGH promotes EMT and invasion through modulation miRNA expression, we performed microarray profiling using MCF-7 cells stably expressing wild type or a translation deficient hGH gene and identified miR-96-182-183 as an autocrine/paracrine hGH regulated miRNA cluster. Forced expression of miR-96-182-183 conferred epithelioid MCF-7 cells with a mesenchymal phenotype and promoted invasive behavior in vitro and dissemination in vivo. Moreover, we observed that miR-96-182-183 promoted EMT and invasion by directly and simultaneously suppressing Breast Cancer Metastasis Suppressor 1-like (BRMS1L) gene expression. miR-96 and miR-182 also targeted GHR, providing a potential negative feedback loop in the hGH-GHR signaling pathway. We further demonstrated that autocrine/paracrine hGH stimulated miR-96-182-183 expression and facilitated EMT and invasion via STAT3 and STAT5 signaling. Consistent with elevated expression of autocrine/paracrine hGH in metastatic breast cancer tissue, miR-96-182-183 expression was also remarkably enhanced. Hence, we delineate the roles of the miRNA-96-182-183 cluster and elucidate a novel hGH-GHR-STAT3/STAT5-miR-96-182-183-BRMS1L-ZEB1/E47-EMT/invasion axis, which provides further understanding of the mechanism of autocrine/paracrine hGH stimulated EMT and invasion in breast cancer.
    Journal of Biological Chemistry 04/2015; 290(22). DOI:10.1074/jbc.M115.653261 · 4.57 Impact Factor
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    ABSTRACT: To analyze the clinical features and mutation of MUT gene in a Chinese patient with isolated methylmalonic acidemia. The clinical characteristics and laboratory tests data were collected. Genomic DNA was extracted from peripheral blood leukocytes. The 13 exons and their flanking sequences of the MUT gene were amplified with polymerase chain reaction and subjected to direct DNA sequencing. The patient has featured failure to thrive, lethargy, seizure, hypotonia, severe ketoacidosis and hyperammonemia. Tandem mass results showed reduction of multiple acylcarnitine. Urine organic acid testing showed pronounced increase in methylmalonate excretion. Homocysteine was normal. The patient showed no response to vitamin B12 treatment. The above results suggested that the patient had isolated methylmalonic acidemia. DNA sequencing analysis confirmed that the patient has carried two MUT gene mutations, c.755dupA and a novel mutation c.944dupT. Inherited metabolic disease screening plays an important role in the diagnosis of clinical diseases. However, to confirm the results will need gene mutation analysis.
    Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 04/2015; 32(2):218-221. DOI:10.3760/cma.j.issn.1003-9406.2015.02.014
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    ABSTRACT: To evaluate the efficiency of one-step multiplex RT-PCR for identifying four common fusion transcripts (TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL) in children with acute lymphoblastic leukemia (ALL). Total RNA was extracted from bone marrow samples of 76 children who were newly diagnosed with ALL between January 2003 and December 2010. These RNAs were analyzed for TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL by one-step multiplex RT-PCR or common nested-multiplex PCR. The PCR products were confirmed by DNA sequencing. TEL/AML1 was found in 12 cases (the length of products was 298 bp in 9 cases and 259 bp in 3 cases), E2A/PBX1 was found in 3 cases (the length of products was 373 bp), BCR/ABL was found in 1 case (the length of products was 2 124 bp), and MLL/AF4 was found in 7 cases (the length of products was 427 bp in 1 case and 673 bp in 6 cases) using one-step multiplex RT-PCR combined with DNA sequencing. The results were consistent with those using common nested-multiplex PCR. One-step multiplex RT-PCR may be another alternative for detection of common fusion transcripts in children with ALL.
    Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics 04/2015; 17(4):332-336. DOI:10.7499/j.issn.1008-8830.2015.04.008
  • Min Zhang · Li Liu · Huifeng Xiao · Ting Zhao · Liuqing Yang · Xiaohua Xu ·
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    ABSTRACT: A series of novel benzo[c]phenanthridine derivatives 2a, 2b, 2c, 2d, 2e, 2f, 2g, 2h, 2i, 2j, 2k, 2l, 2m and 3a, 3b, 3c, 3d, 3e, 3f bearing an alkylamino side chain at their 6-position were synthesized. All of the target compounds were confirmed by 1H NMR, 13C NMR, and HRMS, and some of them were also characterized by IR and 19F NMR. The preliminary bioassays showed that the target compounds displayed fungicidal activities; for example, compound 2l showed 60.0% and 70.0% inhibitive activity against Alternaria solani and Cercospora arachidicola at 50 mg/L, respectively, and some compounds also displayed plant growth-regulating activities.
    Journal of Heterocyclic Chemistry 03/2015; DOI:10.1002/jhet.2410 · 0.79 Impact Factor
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    ABSTRACT: The demand for petroleum has risen rapidly due to increasing industrialization and modernization of the world. The limited reserve of the fossil fuels is also dwindling alongside escalation in the prices. The threats from these and food insecurity are, however, drawing the attention of researchers for alternative fuel which can be produced from renewable feedstocks. Biodiesel as the most promising alternate is currently produced from conventionally grown edible plant oils such as rapeseed, soybean, sunflower and palm. The use of the edible oils is worsening the current competition of oil for food and for fuel. Focus on the use of non-edible resources is presently directed to jatropha, mahua, pongamia, calophyllum tobacco, cotton oil, etc. Discrepancies between the expectation and realities regarding these non-edible oils are necessitating efforts for diversification of the feedstocks to resources that could guarantee energy production without affecting food security. Neem, karanja, rubber and jatropha are evergreen multipurpose non-edible plants that are widely available and can be grown in diverse socio-economic and environmental conditions. These plants are described as golden trees that have multiple uses such as for fuels, medicines, dyes, ornamentals, feeds, soil enrichment, afforestation, etc. This study was therefore undertaken to explore the multipurpose of these four non-edible tree plants. Among the highlights of this expatiate review include oil as feedstock for biodiesel, the need for non-edible feedstocks, neem, karanja, rubber, jatropha and their value chains, methods of modifying oil to biodiesel, factors affecting biodiesel production, application of the selected non-edible seed biodiesels to engines for performance and emission characteristics and the outlook.
    Renewable and Sustainable Energy Reviews 03/2015; 43:495-520. DOI:10.1016/j.rser.2014.11.049 · 5.90 Impact Factor
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    ABSTRACT: To develop a direct microbial sugar conversion platform for the production of lipids, drop-in fuels and chemicals from cellulosic biomass substrate, we chose Yarrowia lipolytica as a viable demonstration strain. Y. lipolytica is known to accumulate lipids intracellularly and is capable of metabolizing sugars to produce lipids; however, it lacks the lignocellulose-degrading enzymes needed to break down biomass directly. While research is continuing on the development of a Y. lipolytica strain able to degrade cellulose, in this study, we present successful expression of several xylanases in Y. lipolytica. The XynII and XlnD expressing Yarrowia strains exhibited an ability to grow on xylan mineral plates. This was shown by Congo Red staining of halo zones on xylan mineral plates. Enzymatic activity tests further demonstrated active expression of XynII and XlnD in Y. lipolytica. Furthermore, synergistic action in converting xylan to xylose was observed when XlnD acted in concert with XynII. The successful expression of these xylanases in Yarrowia further advances us toward our goal to develop a direct microbial conversion process using this organism.
    PLoS ONE 12/2014; 9(12):e111443. DOI:10.1371/journal.pone.0111443 · 3.23 Impact Factor
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    ABSTRACT: Background Yarrowia lipolytica is an oleaginous yeast capable of metabolizing glucose to lipids, which then accumulate intracellularly. However, it lacks the suite of cellulolytic enzymes required to break down biomass cellulose and cannot therefore utilize biomass directly as a carbon source. Toward the development of a direct microbial conversion platform for the production of hydrocarbon fuels from cellulosic biomass, the potential for Y. lipolytica to function as a consolidated bioprocessing strain was investigated by first conducting a genomic search and functional testing of its endogenous glycoside hydrolases. Once the range of endogenous enzymes was determined, the critical cellulases from Trichoderma reesei were cloned into Yarrowia. Results Initially, work to express T. reesei endoglucanase II (EGII) and cellobiohydrolase (CBH) II in Y. lipolytica resulted in the successful secretion of active enzymes. However, a critical cellulase, T. reesei CBHI, while successfully expressed in and secreted from Yarrowia, showed less than expected enzymatic activity, suggesting an incompatibility (probably at the post-translational level) for its expression in Yarrowia. This result prompted us to evaluate alternative or modified CBHI enzymes. Our subsequent expression of a T. reesei-Talaromyces emersonii (Tr-Te) chimeric CBHI, Chaetomium thermophilum CBHI, and Humicola grisea CBHI demonstrated remarkably improved enzymatic activities. Specifically, the purified chimeric Tr-Te CBHI showed a specific activity on Avicel that is comparable to that of the native T. reesei CBHI. Furthermore, the chimeric Tr-Te CBHI also showed significant synergism with EGII and CBHII in degrading cellulosic substrates, using either mixed supernatants or co-cultures of the corresponding Y. lipolytica transformants. The consortia system approach also allows rational volume mixing of the transformant cultures in accordance with the optimal ratio of cellulases required for efficient degradation of cellulosic substrates. Conclusions Taken together, this work demonstrates the first case of successful expression of a chimeric CBHI with essentially full native activity in Y. lipolytica, and supports the notion that Y. lipolytica strains can be genetically engineered, ultimately by heterologous expression of fungal cellulases and other enzymes, to directly convert lignocellulosic substrates to biofuels. Electronic supplementary material The online version of this article (doi:10.1186/s13068-014-0148-0) contains supplementary material, which is available to authorized users.
    Biotechnology for Biofuels 12/2014; 7(1):148. DOI:10.1186/s13068-014-0148-0 · 6.04 Impact Factor
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    ABSTRACT: A multi-step synthesis of 4,6-dimethoxyaurone derivatives was carried out from phloroglucinol and chloroacetonitrile, and the products were characterized by 1H NMR, 13C NMR, and HRMS. The preliminary bioassays showed that some products exhibited herbicidal activity against dicotyledonous plant Brassica campestris L.
    Journal of Heterocyclic Chemistry 12/2014; DOI:10.1002/jhet.2298 · 0.79 Impact Factor
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    ABSTRACT: Background Natural killer (NK) cells are abundant in the liver and constitute a major innate immune component that contributes to immune-mediated liver injury. However, few studies have investigated the phenotypes and functions of NK cells involved in hepatitis B related liver failure (LF), and the precise mechanism underlying NK cell regulation is not fully understood.Methods We detected the percentage and function of peripheral NK cells both in hepatitis B related LF patients and healthy volunteers by flow cytometry and isolated the liver myofibroblasts (LMFs) from hepatitis B related LF livers. To determine the possible effects of LMFs on NK cells, mixed cell cultures were established in vitro.ResultsWe found a down-regulated percentage of peripheral NK cells in hepatitis B related LF patients, and their NK cells also displayed decreased activated natural cytotoxicity receptors (NCRs) and cytokine production. In a co-culture model, LMFs sharply attenuated IL-2-induced NK cell triggering receptors, cytotoxicity, and cytokine production. The inhibitory effect of LMFs on NK cells correlated with their ability to produce prostaglandin (PG) E2.Conclusion These data suggest that LMFs may protect against immune-mediated liver injury in hepatitis B related LF patients by inhibiting NK cell function via PGE2.
    Journal of Translational Medicine 11/2014; 12(1):308. DOI:10.1186/s12967-014-0308-9 · 3.93 Impact Factor
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    ABSTRACT: Objective: Considering inconclusive and heterogenous results, the aim of this study was to investigate the risk of thyroid cancer in systemic lupus erythematosus (SLE) by a quantitatively systematic review with meta-analysis. Methods: Electronic database of PubMed was searched for studies characterizing the associated risk of thyroid cancer in patients with SLE. The meta-analysis procedure was used to combine standardized incidence rates (SIRs) with 95% confidence intervals (CIs) to evaluate the association. Results: Seven cohort studies fulfilled the inclusion criteria and were subjected to the final analysis in the meta-analysis. Homogeneity was confirmed across the included studies. The pooled SIR based on a fixed-effect model was 2.22, with a 95% CI of 2.11-2.34. Sensitivity analyses suggested that the summary statistics obtained should approximate the actual average. Conclusion: Findings from this meta-analysis revealed the positive association between thyroid cancer and SLE risk. Individuals with SLE have a heightened risk of developing thyroid cancer.
    International journal of clinical and experimental pathology 10/2014; 7(9):6270-3. · 1.89 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the mRNA expression levels of suppressor of cytokine signaling 1 (SOCS1) in patients with systemic lupus erythematosus (SLE) compared with healthy controls. The associations of systemic lupus erythematosus disease activity index scores and clinical features of SLE with the expression levels of SOCS1 mRNA were also evaluated. Real-time quantitative reverse transcription-polymerase chain reaction was applied to detect the mRNA expression levels of SOCS1 in peripheral blood mononuclear cells from 34 patients with SLE and 34 healthy controls. The mRNA expression level of SOCS1 was significantly decreased in SLE patients in comparison with healthy controls (Z = −4.207, P < 0.001). Lower SOCS1 mRNA expression was detected in active SLE patients when compared with inactive ones (Z = −2.428, P = 0.015). There was no significant difference found for the SOCS1 mRNA levels between SLE patients with nephritis and those without (Z = −0.642, P = 0.521). The presence of photosensitivity, proteinuria, positive antinuclear antibody, and C4 decline were associated with SOCS1 mRNA levels in SLE patients (all P < 0.05). Furthermore, the SOCS1 mRNA expression was negatively correlated with disease activity (r s = −0.372, P = 0.030). Our results suggest that the dysregulation of SOCS1 might be associated with the pathogenesis of SLE.
    Clinical and Experimental Medicine 10/2014; 15(3). DOI:10.1007/s10238-014-0309-2 · 2.96 Impact Factor
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    ABSTRACT: Africa is a continent full of untapped natural resources ranging from biodiversity to vast water bodies but faced with food and energy crises. Prices of fuel are also escalating. With researchers and experts scrambling for the solution, biodiesel from vegetable oil will receive more attention. But the edible feedstock as the obvious cheapest choice will not be sustainable enough for the increasing energy and food demand; hence, there is a need for guaranteed feedstock. This study was therefore undertaken to explore feedstock that would not be suitable for food but useful for biodiesel in Africa. Among the highlight areas of the study include current energy situation in Africa, technologies of biodiesel production, current state of biodiesel in Africa, driving forces for increase in biodiesel production, current existing problems of biodiesel commercialization, potential benefits of biodiesel processes, need for non-edible oil plants, potential non-edible biodiesel feedstock, biology, distribution and chemistry of the selected non-edible oil plants. The study also throws light on the implication of biodiesel on the environment and the outlook. From the study, the use of non-edible oils can be guaranteed as sustainable feedstock for biodiesel since most of the non-edible plants can be grown on wastelands to reclaim them, not compete with food crops for limited lands, are relatively cheap, available and offer similar or even higher yields of biodiesel and fuel properties as the edibles. Developing biodiesel industry in Africa can help curb the high rate of unemployment through job creation as well as increase in income level of the rural populace. Weaning African economies from oil import dependencies could also be an economic achievement. It can be deduced from the study that there are promising non-edible oil resources in the system for biodiesel industrialization in Africa.
    Renewable and Sustainable Energy Reviews 10/2014; 38:461–477. DOI:10.1016/j.rser.2014.06.002 · 5.90 Impact Factor
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    ABSTRACT: IntroductionRecurrence or early metastasis remains the predominant cause of mortality in patients with estrogen receptor positive (ER+) mammary carcinoma (MC). However, the molecular mechanisms underlying the initial progression of ER¿+¿MC to metastasis remains poorly understood. Trefoil factor 3 (TFF3) is an estrogen-responsive oncogene in MC. Herein, we provide evidence for a functional role of TFF3 in metastatic progression of ER¿+¿MC.Methods The association of TFF3 expression with clinicopathological parameters and survival outcome in a cohort of MC patients was assessed by immunohistochemistry. The expression of TFF3 in MCF7 and T47D cells was modulated by forced expression or siRNA-mediated depletion of TFF3. mRNA and protein levels were determined using qPCR and western blot. The functional effect of modulation of TFF3 expression in MC cells was determined in vitro and in vivo. Mechanistic analyses were performed using reporter constructs, modulation of signal transducer and activator of transcription 3 (STAT3) expression, and pharmacological inhibitors against c-SRC and STAT3 activity.ResultsTFF3 protein expression was positively associated with larger tumour size, lymph node metastasis, higher stage, and poor survival outcome. Forced expression of TFF3 in ER¿+¿MC cells stimulated colony scattering, cell adhesion to a Collagen I-coated matrix, colony formation on a Collagen I- or Matrigel-coated matrix, endothelial cell adhesion, and transmigration through an endothelial cell barrier. In vivo, forced expression of TFF3 in MCF7 cells stimulated the formation of metastatic nodules in animal lungs. TFF3 regulation of the mRNA levels of epithelial, mesenchymal, and metastatic-related genes in ER¿+¿MC cells were consistent with the altered cell behaviour. Forced expression of TFF3 in ER¿+¿MC cells stimulated phosphorylation of c-SRC that subsequently increased STAT3 activity, which lead to the downregulation of E-cadherin. siRNA-mediated depletion of TFF3 reduced the invasiveness of ER¿+¿MC cells.ConclusionsTFF3 expression predicts metastasis and poor survival outcome of patients with MC and functionally stimulates cellular invasion and metastasis of ER¿+¿MC cells. Adjuvant functional inhibition of TFF3 may therefore be considered to ameliorate outcome of ER¿+¿MC patients.
    Breast cancer research: BCR 09/2014; 16(5):429. DOI:10.1186/s13058-014-0429-3 · 5.49 Impact Factor
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    ABSTRACT: A variety of 3-N-substituted amino-6-benzyloxypyridazine derivatives were designed and synthesized in satisfactory yields. Their structures were confirmed by IR, H-1-NMR, and elemental analysis; compound 5j was further determined by X-ray diffraction crystallography. Their herbicidal activities were evaluated through barnyard grass and rape cup tests in laboratory bioassays. Most of the title compounds 5 displayed moderate herbicidal activities against the dicotyledonous plant Brassica campestris L. The most active compounds in the laboratory were also evaluated in the greenhouse.
    Journal of Heterocyclic Chemistry 09/2014; 51(5):1404-1409. DOI:10.1002/jhet.1831 · 0.79 Impact Factor

Publication Stats

735 Citations
217.41 Total Impact Points


  • 2009-2015
    • Jiangsu University
      • School of Chemistry and Chemical Engineering
      Chenkiang, Jiangsu Sheng, China
  • 2013-2014
    • University of Science and Technology of China
      • • School of Life Sciences
      • • Hefei National Laboratory for Physical Sciences at the Microscale
      Luchow, Anhui Sheng, China
    • Sun Yat-Sen University
      • Department of Infectious Diseases
      Shengcheng, Guangdong, China
    • Shenzhen Second People's Hospital
      Shen-ch’üan-shih, Zhejiang Sheng, China
  • 2012-2014
    • Anhui Medical University
      • Department of Epidemiology and Biostatistics
      Luchow, Anhui Sheng, China
    • Sun Yat-Sen University of Medical Sciences
      • Department of Infectious Diseases
      Shengcheng, Guangdong, China
  • 2005-2014
    • Nankai University
      • • Institute of Elemento-Organic Chemistry
      • • State Key Laboratory of Elemento-Organic Chemistry
      T’ien-ching-shih, Tianjin Shi, China
  • 2002-2014
    • National Renewable Energy Laboratory
      • Biosciences Center
      گلدن، کلرادو, Colorado, United States
  • 2008
    • Shenzhen Children's Hospital
      Shechenzhuang, Jiangsu Sheng, China