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ABSTRACT: We report changes in free radical-metabolizing enzymes and the increased generation of lipid peroxides associated with extreme metal accumulation in the liver of the Long-Evans with cinnamon-like coat color (LEC) rat, a new mutant strain displaying hereditary hepatitis and subsequent hepatocellular carcinoma. The activity of free radical-metabolizing enzymes and lipid peroxides, and the concentration of metal in the liver were determined sequentially after birth. Mn-superoxide dismutase activity significantly increased immediately after the onset of hepatitis in LEC rats, whereas no remarkable change was observed in control rats. Cu, Zn-superoxide dismutase activity in LEC rats was similar to that in control rats. Glutathione reductase activity increased, while glutathione peroxidase activity was lower in LEC rats than in control rats throughout the observation periods. Lipid peroxides, estimated by thiobarbituric acid reaction, also increased 4- to 5-fold immediately after the onset of hepatitis in LEC rats. Copper concentration was 30- to 50-fold higher in the liver of LEC rats than in control rats, and the iron content also increased significantly before and after the onset of hepatitis. These findings suggested that an oxidant injury generated by toxic metals could be one of the factors responsible for hepatocellular damage in this unique hereditary hepatitis.
Journal of Gastroenterology 11/1995; 30(5):619-23. · 4.16 Impact Factor
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ABSTRACT: The omentum contains peculiar lymphoid tissues termed omental milky spots. In mice, similar milky spots (splenoportal milky spots) are present in splenoportal fat bands developing along the splenic artery. We found that New Zealand Black (NZB) mice, which are known to develop spontaneous autoimmune diseases, have well developed splenoportal milky spots. However, little is known about these milky spots. Thus we investigated splenoportal fat bands in NZB mice by light and electron microscopy. Splenoportal fat bands contained sporadic aberrant spleens as well as abundant milky spots. In addition, transitional forms between splenoportal milky spots and aberrant spleens, although sporadic, were present in the fat bands. Splenoportal milky spots were supplied with offshoots from the splenic artery and were composed of abundant lymphocytes with macrophages, plasma cells, granulocytes, megakaryocytes and various stromal cells. In addition, they showed active neutrophilic myelopoiesis and probable megakaryopoiesis. Aberrant spleens were also supplied by branches from the splenic artery. They showed active granulopoiesis, megakaryopoiesis, and erythropoiesis. The transitional forms resembled splenoportal milky spots in structure, but the former showed extramedullary haematopoiesis of three cell lineages. The morphological transition from aberrant spleens, via transitional forms, to splenoportal milky spots seems to indicate that splenoportal milky spots represent splenoid lymphoid tissues.
Journal of Anatomy 05/1995; 186 ( Pt 2):287-99. · 2.37 Impact Factor
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ABSTRACT: We carried out a retrospective study for the development of biochemical abnormalities in nine patients with early stages of primary biliary cirrhosis (PBC). All patients had elevated serum alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (gamma-GTP) levels when the diagnosis of PBC was obtained. In all cases, serum gamma-GTP levels initially increased, followed by the elevation of ALP. In the routine laboratory tests gamma-GTP is more sensitive than ALP for the screening for early PBC.
Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 03/1995; 92(2):140-5.
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ABSTRACT: This paper describes ultrasonically-guided infusion of minocycline hydrochloride solution (MINO infusion therapy) in benign nonparasitic cysts of the liver. MINO infusion therapy was performed in 7 large hepatic cysts and successful results were obtained in 6 lesions. The infusion procedure in the initial 4 hepatic cysts a 7 Fr catheter was placed into the cyst and MINO solution was left in the cyst according to the procedure of ethanol infusion therapy. In the most recent 2 cases the cyst was punctured with a 21G needle, washed with physiologic saline and then infused MINO solution without placement of an indwelling drainage tube. This modified procedure is simple and safe and also yields a good therapeutic result. MINO infusion therapy for benign hepatic cyst is very useful and the modified procedure can be performed safely in elderly patients and in patients with several complications.
Nippon Ronen Igakkai Zasshi Japanese Journal of Geriatrics 02/1995; 32(1):33-8.
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ABSTRACT: Therapeutic efficacy of Elental and Enterued as home enteral nutrition (HEN) for the maintenance of remission in 32 patients with quiescent Crohn's disease was studied retrospectively. Their cumulative rates of continuous remission and non hospitalization were statistically compared in relation to following five factors; type of disease, history of bowel resection, duration of disease, oral bile acid tolerance test (OBATT) and radiological lesion score (RLS). As to the cumulative rates of continuous remission and non hospitalization, there was no significant difference between two groups treated with Elental and Enterued. In patient with ileocolitis type, > or = 5-year history, and a high score of RLS, few cases could continuously maintain remission following a long-term HEN. Especially, patients with low absorption in OBATT had a significantly lower cumulative rate of continuous remission than those with normal absorption in OBATT. The present results suggest that above factors may be useful in the application of HEN to patients with Crohn's disease.
Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 02/1995; 92(1):32-40.
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Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 01/1995; 91(12):2264-8.
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American Journal of Hematology 12/1994; 47(3):246-7. · 4.67 Impact Factor
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ABSTRACT: The mouse omentum contains omental milky spots. They are abundantly present particularly in the omental fat band. In normal mice, the milky spots are composed of abundant lymphocytes/plasma cells with macrophages, granulocytes and various stromal cells. Unlike the lymph node, they show occasional neutrophilic myelopoiesis, though neither erythropoiesis nor megakaryopoiesis is present. We investigated the haematopoietic ability of the milky spots in ddY mice by administering intraperitoneal injections of erythropoietin (EPO), 500 units/body/day for 7 consecutive days. The omental fat bands were removed the day after the last EPO injection, and the milky spots were examined by light and electron microscopy. Small clusters of erythroblasts appeared in the milky spots in mice injected with EPO. In these clusters, erythroblasts in various maturation stages, dividing erythroblasts, denucleating erythroblasts and reticulocytes were seen by electron microscopy. These findings suggest that such clusters represent erythropoietic foci. The presence of erythropoietic foci in the milky spots was confirmed in all the mice injected with EPO. Megakaryocytes did not appear in the milky spots. These findings suggest that the milky spots have a latent erythropoietic ability, as well as active neutrophilic myelopoiesis.
International Journal of Experimental Pathology 11/1994; 75(5):375-83. · 2.57 Impact Factor
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ABSTRACT: Unique rounded granules consisting of clustered parallel tubules, 29-31 nm in diameter, with occasional, flocculent or amorphous electron-dense material (parallel tubular granules, PTGs) were found in immature neutrophils in the bone marrow in a case of chronic neutrophilic leukemia (CNL). PTGs were positive for electron microscopic myeloperoxidase. Since no structures similar to PTGs have been documented, we investigated the bone marrow of 65 adult patients with hematologic diseases, and 7 adult patients with miscellaneous non-hematologic diseases, by electron microscopy. The results showed that no PTGs were found in any cases other than the original CNL case. These findings suggest that PTGs are rare granules representing certain primary granules. In addition, the presence of PTGs might be helpful in diagnosing CNL.
Leukemia and Lymphoma 10/1994; 15(1-2):177-86. · 2.58 Impact Factor
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ABSTRACT: A case of splenic vein aneurysm is reported. The patient was diagnosed as having a cirrhotic liver with portal hypertension. Computed tomography and angiography demonstrated a splenic vein aneurysm with saccular dilatation. The splenic vein aneurysm increased in size as the hepatic cirrhosis deteriorated; however, there were no complications such as rupture or thrombosis. To our knowledge this is the first report describing the development of a portal system aneurysm.
British Journal of Radiology 08/1994; 67(799):656-8. · 1.31 Impact Factor
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Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 08/1994; 91(7):1257-61.
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ABSTRACT: Simvastatin (SV), a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, inhibits the synthesis of mevalonic acid. The dose-dependent (0.1-100 micrograms/ml) cytotoxicity of SV towards human (MIAPaCa-2, Panc-1, HPC-1, HPC-3, HPC-4, PK-1, PK-9) and hamster (T2) pancreatic carcinoma cell lines was determined by MTT assay. At up to 20 micrograms/ml of SV, the effect was reversible and was restored by 60 micrograms/ml mevalonic acid. Point mutation of Ki-ras at codon 12 in each cell line was detected by means of the modified polymerase chain reaction. The concentration of SV necessary to achieve 50% cytotoxicity was about 10 micrograms/ml, and at this concentration of SV, DNA synthesis assayed in terms of [3H]thymidine uptake, isoprenylation of p21ras examined by Western blotting and cell progression from G1 to S phase of the cell cycle analyzed by flow cytometry were all inhibited. Isoprenylation inhibitors of p21ras, such as SV, are expected to be useful for the treatment of pancreatic cancer.
Japanese journal of cancer research: Gann 07/1994; 85(6):633-8.
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ABSTRACT: Intramucosal growth patterns of minute superficial colorectal carcinomas removed by endoscopic surgery were analyzed on these microscopic views by using two dimension morphometrical system. Superficial colorectal carcinomas were classified into 2 types based on endoscopic findings; superficial elevated type 21 cases and superficial depressed type 30 cases. Main factors determining growth pattern in mucosa depended on size in superficial elevated type and ratio of transmucosal growth in superficial depressed type. PG type and NPG type showed the same growth pattern in terms of ratio of transmucosal growth and mucosal thickness of central depressive area to the horizontal size. Accordingly, intramucosal growth patterns were different between superficial elevated type and superficial depressed type in minute lesions. And, PG type and NPG type could be included as superficial depressed type.
Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 05/1994; 91(4):854-62.
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ABSTRACT: A case of chronic neutrophilic leukaemia (CNL) is reported. The diagnosis was based on leucocytosis with mature neutrophils, a raised leucocyte alkaline phosphatase score, negative Philadelphia chromosome, and extensive infiltration of neutrophils in various differentiation stages into the bone marrow. When viewed by light microscopy, these neutrophils were almost normal in appearance, except for the presence of ring shaped nuclei and cytoplasmic vacuoles. Electron microscopy showed that neutrophilic promyelocytes and early myelocytes in the bone marrow frequently possessed unique rounded granules consisting of clustered parallel tubules, of 29-31 nm in diameter, and occasional amorphous electron dense material. These parallel tubules showed a hexagonal array; the granules were termed parallel tubular granules (PTGs). PTGs were positive for electron microscopic myeloperoxidase, and were seen exclusively in neutrophilic promyelocytes and early myelocytes. These findings suggest that PTGs represent certain primary granules peculiar to immature neutrophils. Their presence might also be helpful in diagnosing CNL.
Journal of Clinical Pathology 05/1994; 47(4):367-9. · 2.31 Impact Factor
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ABSTRACT: We examined the rhythm of weight velocity in eight patients with Crohn's disease. In this paper we used the spline smoothing technique to study the cycle in the weight increment velocity curves derived from individual data during nutritional therapy. As a result of the weight measurement at four-day intervals, an infradian rhythm with the average cycle of 9.6 +/- 0.7 was recognized in all of the subjects in terms of the weight increment velocity. This rhythm was especially conspicuous in three adolescent patients with serious nutritional lesion. The cycle of the rhythm was unaffected by any of the following factors: energy intake, contents of nutritional therapy, medical examination during the period. Our findings of a common cycle in the process of convalescence from a low nutritious stage in patients with Crohn's disease suggest that, in others as well, the weight increment velocity might have an infradian rhythm with a kind of inherent periodicity.
Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 04/1994; 91(3):250-6.
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ABSTRACT: The left and right dorsal motor nuclei (DMN) separately innervate the anterior and posterior gastric walls through the left and right gastric branches of the vagus nerve (GBVN) in rats. The present study was carried out to investigate the effects of selective centrally originated excitation of the unilateral vagal system on the gastric area in which vagus-induced gastric ulcers developed. Since intracisternally injected thyrotropin-releasing hormone (TRH) stimulates neurons in the bilateral DMNs to produce gastric ulcers, selective stimulation of the unilateral vagal system was produced by contralateral gastric branch vagotomy before intracisternal injection of TRH. Intracisternal injection of TRH (2 micrograms/rat) into left gastric branch-vagotomized rats resulted in lesion formation only on the posterior gastric wall and not on the anterior wall. In contrast, in right gastric branch-vagotomized rats TRH-induced gastric lesions were observed only on the anterior gastric wall and not on the posterior wall. These results suggest that selective stimulation of the left or right DMN induces site-specific ulcer formation through the left or right GBVN. Next, gastric acid secretion was determined in pylorus-ligated rats to examine a role of acid hypersecretion in site-specific ulcer formation caused by TRH. Of interest was that gastric acid secretion in unilaterally vagotomized rats given TRH intracisternally was significantly smaller than that in sham-operated rats given intracisternal saline, although the former rats developed gastric ulcers, whereas the latter did not. It is therefore speculated that gastric hyperacidity plays a less important role in the peripheral mechanisms of TRH-induced site-specific gastric ulceration.
Scandinavian Journal of Gastroenterology 04/1994; 29(3):226-31. · 2.02 Impact Factor
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ABSTRACT: Cryoultramicrotomy applying immunogold staining (immunocryoultramicrotomy) is a useful method to demonstrate the localization of antigens in biological specimens. Recently, we have developed a modified method for immunocryoultramicrotomy using gelatin as an embedding medium (gelatin embedding method). In this study, we compared the results obtained by the gelatin embedding method with those obtained by a conventional method applying polyvinyl alcohol embedding (PVA embedding method). Both methods were easy to perform, and yielded fairly satisfactory results in terms of detecting antigens and preserving ultrastructures. According to the gelatin embedding method, membranes enclosing organelles revealed a negatively stained image, and organelles were clearly delineated by an electron-lucent layer. In contrast, a positively stained image was obtained by the PVA embedding method; however, the delineation of organelles was somewhat inferior to that of the gelatin embedding method. Although these methods were useful to detect various biological antigens, they had some limitations in sensitivity. Prior glutaraldehyde fixation was requisite for cryosectioning and preserving ultrastructures, but it caused the loss of antigenicity to some extent. This should be taken into account when evaluating the results obtained by immunocryoultramicrotomy. In this paper, we present the detailed procedures of the gelatin embedding method, as well as the PVA embedding method, and demonstrate the localization of myeloperoxidase, lysozyme and CD3 molecules in human blood cells using both methods.
[Hokkaido igaku zasshi] The Hokkaido journal of medical science 04/1994; 69(2):249-57.
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ABSTRACT: Omental milky spots are especially large and numerous in New Zealand Black (NZB) mice, which are known to develop spontaneous autoimmune diseases. We investigated omental milky spots in NZB mice by light and electron microscopy. The milky spots were composed of abundant lymphocytes/plasma cells with macrophages, neutrophils, eosinophils, megakaryocytes, and various stromal cells. In addition, clustered neutrophils in various maturation stages with occasional mitotic figures were frequently present in the milky spots: apparent neutrophilic myelopoiesis was present. The presence of megakaryocytes was sporadic. Considering the giant size of megakaryocytes, their direct migration into the milky spots from the bone marrow or spleen seems improbable. Thus, the presence of megakaryocytes was interpreted as probable megakaryopoiesis. Erythroblasts were not contained in the milky spots. These findings seem to indicate that the milky spots in NZB mice represent a special type of lymphoid tissue with active neutrophilic myelopoiesis and probable megakaryopoiesis. Reticulum cells in the milky spots in NZB mice had well-developed dense bodies consisting of clustered parallel tubules that showed a hexagonal array. However, the biological significance of these cells remains unknown.
Anatomy and Embryology 04/1994; 189(3):215-26. · 1.42 Impact Factor
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Nippon Shokakibyo Gakkai zasshi The Japanese journal of gastro-enterology 02/1994; 91(1):89-94.
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British Journal of Dermatology 01/1994; 129(6):746-7. · 3.67 Impact Factor
