Makoto Kobayashi

Tottori University, Tottori, Tottori-ken, Japan

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Publications (2)1.86 Total impact

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    ABSTRACT: Surgery for esophageal cancer is one of the most invasive treatments in gastrointestinal surgery. The aim of this study was to analyze perioperative kinetics of coagulation-fibrinolytic states in comparison with cytokines in order to investigate biological responses to surgical stress in patients undergoing esophagectomy. Serum or plasma samples of 20 patients with esophageal cancer who had undergone esophagectomy were collected during the perioperative period. Serial concentrations of cytokines (IL-6, IL-8, IL-10), PMN-E, and coagulation-fibrinolytic agents (TAT and PIC) were measured and analyzed. There were significant increases in concentrations of both inflammatory and anti-inflammatory cytokines during the operative and postoperative courses. TAT and PIC levels also increased significantly intra- and postoperatively. The TAT/PIC ratio was elevated simultaneously with a predominance of inflammatory cytokines over anti-inflammatory ones. In addition to the profile of cytokines, the TAT/PIC ratio seems to be a sensitive and useful indicator in evaluating biological responses to surgical stress.
    Hepato-gastroenterology 09/2004; 51(59):1376-8. · 0.93 Impact Factor
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    ABSTRACT: Endotoxin-induced liver failure is characterized by necrosis and apoptosis of hepatocytes. The aim of this study was to establish the relation of the time-course of hepatic parenchymal cell apoptosis and necrosis in a murine endotoxin shock model and to characterize the role of the gene products of Bcl-2 and Bax in endotoxin-mediated hepatocellular apoptosis. Male Wistar rats were treated with 5 mg/kg body weight endotoxin, and the necrosis and apoptosis of hepatocytes were assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining and enzyme-linked immunosorbent assay for DNA fragmentation. Furthermore, Bcl-2 and Bax were analyzed by Western blotting. Endotoxin treatment led to liver dysfunction, and increased hepatic parenchymal cell apoptosis. Necrotic changes in hepatic tissues were found after the appearance of apoptosis. The apoptotic index increased up to 12 hours after endotoxin treatment. Western blot analysis revealed that the apoptosis was accompanied by a decrease of Bcl-2 protein and a relative increase of Bax protein. The apoptotic index decreased at 24 hours after the treatment synchronously with an increase in the ratio of Bcl-2 and Bax. These results demonstrate a significant role of hepatocellular apoptotic processes in the later development of necrosis in the liver. The kientics of this apoptosis are directly proportionate to the disturbed ratio of Bcl-2 and Bax.
    Hepato-gastroenterology 01/2002; 49(48):1602-6. · 0.93 Impact Factor

Publication Stats

9 Citations
1.86 Total Impact Points

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  • 2002
    • Tottori University
      • Faculty of Medicine
      Tottori, Tottori-ken, Japan