M Sano

Hamamatsu University School of Medicine, Hamamatu, Shizuoka, Japan

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Publications (28)21.07 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Effects of artemether were examined on Schistosoma japonicum in mice. When the drug was given at a daily dosage of 200 mg/kg for 4 successive days from 46 days post-infection, a significant reduction in worm recovery was observed. A significant reduction in size of worms from the medicated mice was also seen compared with that from non-medicated controls.
    The Southeast Asian journal of tropical medicine and public health 04/1993; 24(1):53-6. · 0.61 Impact Factor
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    ABSTRACT: Mebendazole was given to mice infected with Angiostrongylus costaricensis at a single dose of 5 mg/kg at 6, 11, 16 or 21 days post-infection (p.i.) and in five successive doses at 5 mg/kg daily at 6, 11 or 16 days p.i. The effects were comparatively assessed by examining various parameters in host mice and worms. As a whole, the effects of mebendazole were caused more conspicuously by five successive treatments than by a single treatment. In both treatment modalities, the effects were more remarkable in earlier treatments, and nearly complete effects were caused by five successive treatments before 15 days p.i. These results suggest that the inhibition of egg formation and/or oviposition will inhibit the pathological changes caused in the disease by A. costaricensis, especially before the onset of the changes.
    Parasitology Research 02/1993; 79(6):441-3. · 2.85 Impact Factor
  • A Ishih, M Nishimura, M Sano
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    ABSTRACT: Experimental Hymenolepis diminuta infection was carried out in inbred strains of rats (F344/N, JAR-2, LOU/M, TM, DA and DA-bg/bg) and outbred Wistar rats. All strains became infected with this cestode, but clear strain-dependent variation in the susceptibility to H. diminuta infection was observed. Marked differences in worm persistence and worm weight were found at 6 weeks post-infection in TM and DA rats. These strains would be useful to clarify the interactions between H. diminuta and its rat host.
    Journal of Helminthology 07/1992; 66(2):132-6. · 1.16 Impact Factor
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    ABSTRACT: The surfaces of larval and adult Angiostrongylus costaricensis, causative agent of human abdominal granuloma, were studied by the use of scanning electron microscopy. Cuticular annulations were clearly demonstrated on the surface of larvae and adults. Differences in the appearance of alae in larval stages and in the shape of the tail at different stages of development are described and illustrated. Several aspects of morphology previously unreported for this parasite are also described.
    The Southeast Asian journal of tropical medicine and public health 01/1991; 21(4):568-73. · 0.61 Impact Factor
  • A I Ishii, M Sano
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    ABSTRACT: Experimental Angiostrongylus costaricensis infection was carried out in inbred strains of mice (C57BL/6 BALB/c, DBA/2 and C3H/He). All strains became infected with this parasite. Marked differences in mortality and in worm burden were found among inbred strains of mice tested. A significant reduction was shown in worm length from mice compared to that from cotton rats.
    Journal of Helminthology 01/1990; 63(4):302-6. · 1.16 Impact Factor
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    ABSTRACT: Anti-larval effects of levamisole were examined on A. cantonensis in rats and A. costaricensis in mice. 1) In rats inoculated with 40 infective larvae of A. cantonensis: Compared with a non-treated control group, a significant reduction in number of worms recovered was seen in the group receiving a single dose of 1.0 mg/kg or more. A significant decrease in host lung-body weight ratio was seen in the group receiving drug of 3.0 mg/kg or more. 2) In mice inoculated with 20 infective larvae of A. costaricensis. In the non-treated control group, a severe loss in body weight and death of host animals were observed. A single dose of 30 mg/kg on 3, 4 or 5 days post-infection remarkably inhibited these changes. At 30 mg/kg for 3 or 7 days levamisole was more effective than a single dose of the drug. These results suggest that levamisole has conspicuous in vivo effects against larval stages of A. costaricensis as well as A. cantonensis.
    The Southeast Asian journal of tropical medicine and public health 04/1989; 20(1):109-17. · 0.61 Impact Factor
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    ABSTRACT: The nature of antibody responses were investigated in mice experimentally infected with ten third stage larvae of Angiostrongylus costaricensis. An indirect enzyme-linked immunosorbent assay (ELISA) was designed to detect the circulating antibodies using adult somatic (SA) and excretory-secretory (ES) antigens. Antibodies to SA antigens could be detected at low level from the second week of infection but rose gradually from week 3 until week 5. Antibodies to ES antigens could be detected at higher levels from week 2 and continued rising to reach a peak at week 4. Mebendazole showed some in vitro effect on adult worms but no such effect was found with thiabendazole. There was no difference in the antibody level between just before treatment and five weeks post infection.
    Serodiagnosis and Immunotherapy in Infectious Disease 01/1989; 3(1):51-56.
  • A I Ishii, M Terada, M Sano
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    ABSTRACT: The effects of neuropharmacological agents on the motility of irradiated and non-irradiated Angiostrongylus cantonensis adult females were studied. GABA induced complete paralysis in non-irradiated and 5,000 R-irradiated worms, but caused only slight paralysis on 10,000 R-irradiated worms. The paralytic effect of GABA was antagonised by picrotoxin. The reason for low susceptibility of heavily irradiated worms to GABA is not known. There was no difference in susceptibility of non-irradiated and irradiated worms to other neuropharmacological agents including eserine, phenylephrine and dibenamine.
    The Southeast Asian journal of tropical medicine and public health 01/1988; 18(4):547-51. · 0.61 Impact Factor
  • A I Ishii, M Honda, M Sano
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    ABSTRACT: Effects of gamma ray irradiation on the first-stage larvae of Angiostrongylus cantonensis were studied. Compared to the non-treated controls, infection of rats with third-stage larvae which developed from irradiated first-stage larvae resulted in reduced recovery rates of adults. There was also a change in the male-female adult worm ratio and a reduction in larval output per female in relation to increasing irradiation dosage. Morphological changes in the adults were noted.
    Parasitology Research 02/1987; 73(2):159-64. · 2.85 Impact Factor
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    ABSTRACT: Effects of milbemycin D against adult Angiostrongylus cantonensis in rats were examined. The first-stage larval counts in rat faeces (larvae per gram of faeces per female worm recovered, LPG/female) were most conspicuously reduced in the group treated with nine consecutive weekly doses of 5.0 mg/kg. The effect was more marked in the group treated with five or ten successive daily doses of 5.0 mg/kg than the group treated with a single dose of 25.0 or 5.0 mg/kg. Host lung-body weight ratio and number of recovered worms were reduced significantly only in the group treated with five or ten successive daily doses of 5.0 mg/kg. These results suggest that the action of milbemycin D on the reproductive system of the worms might be differentiated from its killing action. The in vitro motility of females recovered from rats medicated with nine consecutive weekly doses of 5.0 mg/kg was inhibited, and almost all females and males were semitransparent and colourless. Results obtained from sectioned worms showed little content in their digestive tracts and uteri. In addition, there were few eggs and first-stage larvae in the lung tissues of treated rats. These suggest that milbemycin D affects the reproductive functions of the worms through an indirect mode of action including paralysis and inhibition in food intake and energy and/or synthetic metabolism.
    Parasitology Research 02/1987; 73(4):375-80. · 2.85 Impact Factor
  • A I Ishii, M Honda, M Sano
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    ABSTRACT: Effects of gamma-ray irradiation on the first-stage larvae of Angiostrongylus cantonensis were studied. There was a significant reduction in the infectivity of the 10,000 R-irradiated larvae to intermediate host snails compared to other groups. In the final host infection, both the worm populations and worm body length of irradiated groups differed markedly from those of the non-irradiated control.
    Zeitschrift für Parasitenkunde 02/1986; 72(3):331-4.
  • M Terada, M Sano
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    ABSTRACT: Effects of piperazine derivatives, especially of diethylcarbamazine (DEC) on adult Angiostrongylus cantonensis and Dirofilaria immitis were examined. Piperazine (3 X 10(-5)-10(-4) M) paralyzed A. cantonensis and the action was antagonized by picrotoxin. 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP) (10(-5)-10(-4) M) caused contraction but little effect was produced by strychnine. An inhibitory effect on untreated preparations was caused by lower concentrations (3 X 10(-6)-10(-5) M) of diethylcarbamazine citrate (DEC) and also on the preparations contracted by eserine. A stimulatory effect was also seen when higher concentrations (10(-4)-3 X 10(-4) M) of this drug were applied to both preparations. The inhibitory action of DEC was antagonized by gabergic antagonists such as picrotoxin and bicuculline, but not by alpha-adrenergic antagonists like dibenamine and phentolamine. When the worm preparation was paralyzed by strychnine or hexylresorcinol (inhibitors of the release of acetylcholine in this worm), the stimulatory effect of DEC was blocked, but pyrantel (a nicotinic cholinergic agonist) contracted the paralyzed preparation. However, the effect of DEC on D. immitis (10(-7)-3 X 10(-4) M) was inhibitory, and this action was also antagonized by picrotoxin. These results suggest that the DEC inhibitory and stimulatory action is through the gabergic and cholinergic mechanisms in adult A. cantonensis and D. immitis.
    Zeitschrift für Parasitenkunde 02/1986; 72(3):375-85.
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    ABSTRACT: Paralysis due to avermectin B1a and ivermectin of Angiostrongylus cantonensis was compared to that of phenylephrine (an alpha-adrenergic agonist) and strychnine (a cholinergic inhibitor). The paralyzing action of ivermectin (2.5 X 10(-9) g/ml) was inhibited by the single, simultaneous addition of picrotoxin (3 X 10(-5) M), whereas the effect of the drug (2.5 X 10(-7) g/ml) was reversed only when picrotoxin was given with cholinergic spasmogens such as pyrantel and eserine. Bicuculline (3 X 10(-5) M) had a similar antagonistic effect for picrotoxin, but bicuculline was less effective. The paralyzing action of avermectin B1a (3.6 X 10(-14) M, 3.0 X 10(-14) g/ml) was antagonized only when picrotoxin was given with cholinergic spasmogens such as pyrantel, eserine, and N-methylcytisine (N-MC), or alpha-adrenergic antagonists such as phentolamine and dibenamine. On the other hand, the paralyzing action of strychnine (3 X 10(-6) M) or phenylephrine (3 X 10(-5) M) was relatively uninfluenced by picrotoxin, but was antagonized by pyrantel and N-MC or dibenamine. These results suggest that a gabergic mechanism is involved in the paralyzing action of ivermectin, as well as avermectin B1a, in A. cantonensis.
    Experimental Parasitology 05/1984; 57(2):149-57. · 2.15 Impact Factor
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    ABSTRACT: Pyrantel tartrate caused spastic paralysis through stimulating nicotinic cholinoceptors in Angiostrongylus cantonensis.
    Experientia 01/1984; 39(12):1383-5.
  • M Terada, Y Fujiu, M Sano
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    ABSTRACT: Pyrantel tartrate caused spastic paralysis in various parasitic nematodes, but not in cestodes and trematodes.
    Experientia 10/1983; 39(9):1020-2.
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    ABSTRACT: Effects of various cholinergic agents on the motility of Angiostrongylus cantonensis were studied to define the neuropharmacological properties of this worm. Stimulation of the motility and/or contraction were shown by eserine, ACh, carbachol, nicotine, DMPP, pyrantel, and Ba2+, but not by pilocarpine and McN-A-343. Contraction was similarly observed by these agents in the preparations paralyzed with praziquantel. Paralysis was caused remarkably by d-tubocurarine and slightly by succinylcholine, while the contraction induced by eserine and DMPP was little influenced by these drugs. Both the motility and the eserine-induced contraction were little influenced by hexamethonium, but stimulated remarkably by atropine. Though hemicholinium-3, morphine, and picrate showed little effect, guanidine stimulated remarkably the motility and also the eserine-induced contraction. The stimulatory action of guanidine was antagonized by strychnine. Strychnine paralyzed the motility, and the eserine-induced contraction was antagonized by the pre- and post-treatment with strychnine. From these results, it is suggested that the excitatory cholinergic mechanism in A. cantonensis is nicotinic, and it is basically similar to that reported in Ascaris suum.
    The Japanese Journal of Pharmacology 09/1982; 32(4):633-42.
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    ABSTRACT: Effects of some possible neurotransmitters such as GABA, adrenergic drugs, and 5-HT and their antagonists on the motility of Angiostrongylus cantonensis were studied. Paralysis was caused by GABA, avermectin BIa (Av-BIa), piperazine and alpha-adrenergic agonists such as adrenaline, noradrenaline, phenylephrine, clonidine and methoxamine, but not by beta-adrenergic agonists such as isoproterenol. The paralysis by GABA or Av-BIa was antagonized by GABA antagonists such as picrotoxin and/or bicuculline with cholinergic agents such as N-methylcytisine (N-MC) or eserine. The paralysis elicited by alpha-adrenergic agonists was antagonized by alpha-adrenergic antagonists such as phentolamine and dibenamine, but not by beta-adrenergic antagonists such as propranolol. 5-HT affected the motility of A. cantonensis paralytically or spastically. The paralysis induced by 5-HT was antagonized by alpha-adrenergic antagonists such as phentolamine and dibenamine, while the contraction induced by this compound was further stimulated by N-MC, but antagonized by strychnine. Other agents such as glutamine, glycine, aspartic acid, taurine, and substance P showed little effect on the motility of A. Cantonensis. From these findings on the neuropharmacological properties of A. cantonensis, it is suggested that this worm is useful as an excellent nematodal model for the investigation of anthelminthics. In addition, this worm may also useful as one of screening models of drugs affecting the central nervous system in mammals.
    The Japanese Journal of Pharmacology 09/1982; 32(4):643-53.
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    ABSTRACT: Effects of various neuropharmacological agents on the motility of Dipylidium caninum was studied. 5-HT stimulated the motility of D. caninum, while other drugs such as GABA, adrenaline, phenylephrine, isoproterenol, dibenamine, and propranolol showed little effect. The stimulatory action of 5-HT was antagonized by tryptophol. Stibnal caused paralysis which was blocked by the pretreatment with 5-HT. Paralytic effects were caused by eserine, dichlorovos, ACh, carbachol and DMPP, but not by pilocarpine and McN-A-343. d-Tubocurarine, hexamethonium, and atropine all showed little effect on the motility and on the paralytic action of eserine. Though guanidine showed little effect, strychnine remarkably stimulated the motility. The action of strychnine was stimulated by 5-HT but partially inhibited by tryptophol. Morphine and picrate slightly stimulated the motility, but showed little influence on the paralytic action of eserine. These two agents stimulated the paralyzed preparation by tryptophol transiently and sustainedly, respectively. These results on the cholinergic and serotonergic drugs basically showed good agreement with those reported in trematodes such as Schistosoma mansoni and Fasciola hepatica.
    The Japanese Journal of Pharmacology 07/1982; 32(3):479-88.
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    ABSTRACT: Summary Praziquantel (PQ) caused spastic and/or paralytic actions on the motility of various parasitic helminths and isolated host tissue preparations, through a neuropharmacological mechanism.
    Experientia 06/1982; 38(5):549-53.
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    ABSTRACT: Summary Niclosamide, a well-known antitapeworm drug, caused spastic and/or paralytic actions on the motility of various preparations including parasitic cestodes, trematodes, nematodes, and host isolated tissues.
    Experientia 06/1982; 38(5):547-9.