M Miyaji

Publications (2)7.49 Total impact

• Source

  Available from: kmu-ac.jp

  Article: IL-4-producing CD8(+) T cells may be an immunological hallmark of chronic GVHD.

  K Nakamura, R Amakawa, M Takebayashi, Y Son, M Miyaji, K Tajima, K Nakai, T Ito, N Matsumoto, K Zen, Y Kishimoto, S Fukuhara
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  ABSTRACT: Chronic graft-versus-host disease (cGVHD) occurs in approximately 60-80% of those who survive over 100 days after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, the pathophysiology of cGVHD is poorly understood. To gain more insight into the immunological mechanism of cGVHD, we examine cytokine production of peripheral blood T cells from 19 patients in the chronic phase of allo-HSCT. The percentage of IFN-gamma-producing CD8(+) T cells among CD8(+) T cells was significantly higher in patients with or without cGVHD than in normal control subjects (P<0.001). On the other hand, the percentage of IL-4-producing CD8(+) T cells among CD8(+) T cells was significantly higher in patients with cGVHD (mean 3.3%; range 1.3-8.2%) than in patients without cGVHD (mean 1.2%; range 0.8-1.7%) and normal control subjects (mean 1.1%; range 0.1-1.6%) (both P<0.001). By contrast, the percentage of IL-4-producing CD4(+) T cells was not different among patients with and without cGVHD and normal controls. These findings suggest that IL-4-producing CD8(+) T cells may be an immunological marker of cGVHD.
  Bone Marrow Transplantation 10/2005; 36(7):639-47. · 3.75 Impact Factor
• Source

  Available from: kmu-ac.jp

  Article: Blood dendritic cells are decreased in acute graft-versus-host disease.

  M Takebayashi, R Amakawa, K Tajima, M Miyaji, K Nakamura, T Ito, N Matsumoto, Y Miyazaki, K Zen, Y Kishimoto, S Fukuhara
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  ABSTRACT: The recipients of allogeneic hematopoietic stem cell transplantation (allo-HSCT) often develop acute graft-versus-host disease (aGVHD), which is closely related to morbidity and mortality. However, the essential part of the immune responses elicited in aGVHD remains largely unknown. We attempt to determine if peripheral blood dendritic cells (PBDCs) are altered in aGVHD, and find that the number of PBDCs (both myeloid and lymphoid DCs) is significantly decreased. Immunohistochemical staining of the biopsied skin from patients with aGVHD demonstrates that a number of fascin(+) cells with dendritic projections infiltrate the dermis of the skin. Based on these findings, we hypothesize that the PBDCs are recruited to the affected tissues and may thus play important roles in immune responses elicited in aGVHD.
  Bone Marrow Transplantation 06/2004; 33(10):989-96. · 3.75 Impact Factor