B Cardinaud

French National Centre for Scientific Research, Lutetia Parisorum, Île-de-France, France

Are you B Cardinaud?

Claim your profile

Publications (18)62.62 Total impact

  • Annals of the New York Academy of Sciences 12/2006; 812(1):141 - 143. DOI:10.1111/j.1749-6632.1997.tb48154.x · 4.31 Impact Factor
  • Annals of the New York Academy of Sciences 02/2006; 839(1):47 - 52. DOI:10.1111/j.1749-6632.1998.tb10731.x · 4.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A comparative analysis of the structure of the melanin-concentrating hormone (MCH) precursor reveals that this sequence has been subjected to a higher selection pressure in mammals than in teleosts, suggesting that the structural constraints have not been the same throughout the vertebrate lineage. In contrast, the MCH peptide sequence has been very well conserved in all species. A sensitive and reproducible eel skin assay was developed and allowed us to define the structural features needed for a full MCH bioactivity. It was shown that the minimal structure carrying the critical residues was the same in fishes and in mammals. A pharmacological approach confirmed that MCH receptor activation decreased the cAMP levels in the fish skin, but this effect appeared to be independent from a Galphai protein. We propose that one of the intracellular signaling pathways of the MCH receptor in fish skin is the activation of one or several cellular phosphodiesterases.
    Peptides 11/2004; 25(10):1623-32. DOI:10.1016/j.peptides.2004.05.020 · 2.61 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The melanin-concentrating hormone (MCH) gene encodes two proteins, pro-MCH and MCH-gene-overprinted polypeptide (MGOP), produced through alternative splicing of the primary transcript. Our initial purpose was to characterize the MGOP-immunoreactive material. First, MGOP mRNA was clearly found in rat and mouse hypothalami but Western blot analysis failed to unambiguously identify MGOP in protein extracts. Immunohistochemical experiments with wild-type and MCH gene-null mice demonstrated genuine expression of MGOP confined to the MCH-containing neurons in the lateral hypothalamus area and the presence of an 'MGOP-like' antigen in periventricular nucleus and arcuate nucleus neurons and their area of projection. This suggested a colocalization in somatostatin (SRIF) hypophysiotropic neurons. Further characterization, using SRIF gene-null mice and Western blot analysis with recombinant proteins, revealed that the MGOP-like product was pro-SRIF1-64. The role of pro-SRIF1-64 on fetal hypothalamic neurons was evaluated and a strong tonic inhibitory effect on SRIF secretion was found. These results (i) indicate that MGOP expression is restricted to the MCH neurons in the lateral hypothalamus and that MGOP-like immunoreactivity outside this system corresponds to pro-SRIF1-64, and (ii) provide the first evidence for a negative feedback regulation by pro-SRIF1-64 on SRIF secretion, suggesting new mechanisms by which the pro-region of a neuropeptide precursor may control the regulated secretion of a neuropeptide derived from the same precursor.
    European Journal of Neuroscience 03/2004; 19(4):925-36. DOI:10.1111/j.0953-816X.2004.03187.x · 3.67 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Melanin-concentrating hormone (MCH) is a cyclic peptide, mainly involved in the regulation of skin pigmentation in teleosts and feeding behavior in mammals. The human keratinocyte SVK14 cell line has been previously shown to express binding sites for the MCH analog [125I]-[Phe13,3-iodo-Tyr19]MCH. We report here that: (1) this binding site similarly recognized [125I]-[3-iodo-Tyr13]MCH; (2) its pharmacological profile clearly differed from those observed at the two human MCH receptor subtypes, MCH1-R and MCH2-R; (3) MCH did not induce any effect on second messenger systems (including cAMP, calcium, and MAP kinase signaling pathways), and (4) no mRNAs corresponding to the MCH receptors were found. In conclusion, the binding site characterized in the SVK14 cell line is distinct from the MCH1 and MCH2 receptors and deserves therefore further investigation.
    Biochemical and Biophysical Research Communications 08/2002; 295(4):841-8. DOI:10.1016/S0006-291X(02)00763-5 · 2.28 Impact Factor
  • T Suply · B Cardinaud · S Kanamori · C Dal Farra · S Ricois · J L Nahon
    Annals of the New York Academy of Sciences 11/1999; 885:455-8. DOI:10.1111/j.1749-6632.1999.tb08711.x · 4.31 Impact Factor
  • Annals of the New York Academy of Sciences 06/1998; 839:47-52. · 4.31 Impact Factor
  • Annals of the New York Academy of Sciences 05/1998; 839:529-531. DOI:10.1111/j.1749-6632.1998.tb10861.x · 4.31 Impact Factor
  • H B Niznik · F Liu · K S Sugamori · B Cardinaud · P Vernier
    Advances in pharmacology (San Diego, Calif.) 02/1998; 42:404-8.
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this chapter a comparative approach of the dopamine 1 (D1)-receptor class has been undertaken to analyze in detail the dopamine-receptor multiplicity of D1-like receptors in vertebrates by cloning the corresponding genes in most of the main groups of vertebrates. The aim of the chapter is to identify the events that led to the emergence of a “new” D1-receptor gene in the evolution of vertebrates. This phylogenetical approach provides conclusive evidence for the existence of two other subtypes of D1 receptors, one named D1C, found in all the jawed vertebrates except mammals, and one subtype termed D1D, only to be found in birds. Therefore, the D1-receptor class comprises three to four subtypes in “higher” vertebrates, virtually indentical to other catecholamine receptor families, indicating that the gene duplications at the origin of this receptor multiplicity occurred before or concomitantly with the appearance of Chondrychtians (cartilaginous fish). As all of the known bioamine receptor subtypes are expressed in the central nervous system, it is proposed that the bioamine and dopamine D1-receptor diversification accompanies in some respect the genetic mechanisms leading to the encephalization of the vertebrate nervous system. Acquisition and changes in the expression pattern of bioamine receptors is likely to have been the major factor of duplicated gene conservation in vertebrates.
    Advances in pharmacology (San Diego, Calif.) 02/1998; 42:936-40. DOI:10.1016/S1054-3589(08)60901-1
  • J D Vincent · B Cardinaud · P Vernier
    [Show abstract] [Hide abstract]
    ABSTRACT: The evolving vertebrate nervous system was accompanied by major gene duplication events generating novel organs and a sympathetic system. Vertebrate neural pathways synthesizing catecholamine neurotransmitters (dopamine and noradrenaline), were subsequently recruited to process increased information demands by mediating psychomotor functions such as selective attention/predictive reward and emotional drive via the activation of multiple G-protein linked catecholamine receptor subtypes. Here we show that the evolution of these receptor-mediated events were similarly driven by forces of gene duplication, at the cephalochordate/vertebrate transition. In the cephalochordate Amphioxus, a sister group to vertebrates, a single catecholamine receptor gene was found, which based on molecular phylogeny and functional analysis formed a monophyletic group with both vertebrate dopamine D1 and beta adrenergic receptor classes. In addition, the presence of dopamine but not of noradrenaline was assayed in Amphioxus. In contrast, two distinct genes homologous to jawed vertebrate dopamine D1 and beta adrenergic receptor genes were extant in representatives of the earliest craniates, lamprey and hagfish, paralleling high dopamine and noradrenaline content throughout the brain. These data suggest that a D1/beta receptor gene duplication was required to elaborate novel catecholamine psychomotor adaptive responses and that a noradrenergic system specifically emerged at the origin of vertebrate evolution.
    Bulletin de l'Académie nationale de médecine 02/1998; 182(7):1505-14; discussion 1515-6. · 0.22 Impact Factor
  • Biology of the Cell 01/1998; 90(1):128-128. DOI:10.1016/S0248-4900(98)80325-0 · 3.87 Impact Factor
  • P Vernier · B Cardinaud · H Philippe · J D Vincent
    Annals of the New York Academy of Sciences 06/1997; 812:141-3. · 4.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The existence of dopamine D1C and D1D receptors in Xenopus and chicken, respectively, challenged the established duality (D1A and D1B) of the dopamine D1 receptor class in vertebrates. To ascertain the molecular diversity of this gene family in early diverging vertebrates, we isolated four receptor-encoding sequences from the European eel Anguilla anguilla. Molecular phylogeny assigned two receptor sequences (D1A1 and D1A2) to the D1A subtype, and a third receptor to the D1B subtype. Additional sequence was orthologous to the Xenopus D1C receptor and to several other previously unclassified fish D1-like receptors. When expressed in COS-7 cells, eel D1A and D1B receptors display affinity profiles for dopaminergic ligands similar to those of other known vertebrate homologues. The D1C receptor exhibits pharmacological characteristics virtually identical to its Xenopus homologue. Functionally, while all eel D1 receptors stimulate adenylate cyclase, the eel D1B receptor exhibits greater constitutive activity than either D1A or D1C receptors. Semiquantitative reverse transcription-polymerase chain reaction reveals the differential distribution of D1A1, D1A2, D1B, and D1C receptor mRNA within the hypothalamic-pituitary axis of the eel brain. Taken together, these data suggest that the D1A, D1B, and D1C receptors arose prior to the evolutionary divergence of fish and tetrapods and exhibit molecular, pharmacological, and functional attributes that unambiguously allow for their classification as distinct D1 receptor subtypes in the vertebrate phylum.
    Journal of Biological Chemistry 02/1997; 272(5):2778-87. DOI:10.1074/jbc.272.5.2778 · 4.57 Impact Factor
  • P Vernier · B Cardinaud · O Valdenaire · H Philippe · J D Vincent
    [Show abstract] [Hide abstract]
    ABSTRACT: The large molecular diversity of receptors and their subtypes means that the pharmacologist is faced with many puzzling characterization questions. First, the molecular diversity of the receptors is deciphered only in part by a pharmacological approach, which precludes a satisfactory receptor classification based solely on pharmacological characteristics. Second, the physiological counterpart of the numerous subtypes of receptors specifically activated by single endogenous ligands remains unclear. Here, Philippe Vernier and colleagues use the example of the bioamine G protein-coupled receptors to show that many of the apparent inconsistencies that emerge from pharmacological and molecular characterizations of receptors can be better understood if the evolutionary history of the receptors is taken into account.
    Trends in Pharmacological Sciences 12/1995; 16(11):375-81. DOI:10.1016/S0165-6147(00)89078-1 · 9.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Ion-selective microelectrodes were used to measure extracellular free ion concentrations in the retina of the drone honeybee, Apis mellifera male. Mean values were (in millimoles per litre): Na+, 196; K+, 10.2; Ca2+, 2.0; pH 6.9. The elemental composition of fluid that rose into a micropipette inserted in the retina was obtained by electron microprobe X-ray analysis: from the concentrations of Na and K it was estimated that this fluid was 91% interstitial fluid. Amino acids and carbohydrates were analysed by chromatography. Four amino acids had concentrations > 20 mM: proline (109 mM), glutamine (38 mM), alanine (31 mM) and beta -alanine (24 mM). These concentrations were higher than in the haemolymph. Other amino acids had concentrations of less than 3 mM. The identified carbohydrates were trehalose, glucose, pyruvate and fructose. All of these were less concentrated than in the haemolymph. These results: (i) show that the ion concentrations of previously used Ringer solutions were reasonably correct; (ii) demonstrate properties of the blood-retina barrier; (iii) suggest that the extracellular concentration of alanine is ample for it to serve as a major substrate of neuronal energy metabolism in this tissue.
    Proceedings of the Royal Society B: Biological Sciences 07/1994; 257(1348):49-58. DOI:10.1098/rspb.1994.0093 · 5.29 Impact Factor
  • Source
    B Quérat · B Cardinaud · A Hardy · B Vidal · G D'Angelo
    [Show abstract] [Hide abstract]
    ABSTRACT: cDNA clones encoding the European eel (Anguilla anguilla L.) prolactin were isolated from a pituitary cDNA library constructed in gamma gt10, using a rainbow trout Prl cDNA fragment as a probe. Four different inserts were subcloned into the pGEM 3Z plasmid after PCR amplification. The 1082 bp-long nucleotide sequence revealed an open reading frame of 627 bp encoding a 24 amino acid-long signal peptide followed by a 185 amino acid-long mature protein. Comparison studies showed 60-70% homology with other known teleost fish prolactins and 30-45% with non-teleost fish, amphibian, reptilian, avian and mammalian prolactins. In situ hybridization studies using labelled prolactin RNA probe showed a strong signal in the rostral pars distalis of the pituitary gland. We next examined the physiological regulation of this prolactin synthesis in vivo using Northern blot analysis and prolactin cDNA probe labelled by random priming. The pituitary prolactin mRNA level was markedly decreased 3 weeks after transfer of eels from freshwater to sea water. Implants of thyroid hormones left for up to three weeks were ineffective on prolactin mRNA. Estradiol administered as implant, alone or in combination with 500 micrograms testosterone, was also unable to significantly alter the pituitary mRNA level for prolactin in the freshwater silver eels whatever the dose used (20-500 micrograms) and whatever the duration of treatment (from 4 days to 10 weeks).
    Molecular and Cellular Endocrinology 07/1994; 102(1-2):151-60. DOI:10.1016/0303-7207(94)90108-2 · 4.24 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Three subtypes of D1-like receptors in the brain of the European eel, Anguilla anguilla, were isolated through cDNA cloning. Two D1a-like transcripts (Anguilla D1 and D1 PCR) and one D1b/D5-like transcript (Anguilla D5) are expressed. In situ hybridization performed with the Anguilla D1 PCR cRNA as a probe revealed that the corresponding mRNA occurs in most of the dopamine target areas and mainly in regions near the ventricles.
    Netherlands Journal of Zoology 12/1993; 45(1-2):235-237. DOI:10.1163/156854295X01004