M Fazio

Istituto Dermopatico dell'Immacolata, Roma, Latium, Italy

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Publications (37)48.93 Total impact

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    ABSTRACT: Background/aims: Comeometry represents a useful tool for dermatologists, even though several interfering variables may reduce its reliability level. The present study describes some of the methodological points that optimize corneometric measurements and analyzes the interference due to the skin area selected, the age and the sex of the individuals observed.Results: The results show that skin areas may present significantly different corneometric values. In addition, a significant correlation of the corneometric values at most of the sites was observed independently of the sun-exposed site.Conclusions: The corneometric values were clearly age dependent and sex independent. Measurements were obtained from eight different body areas (two of them were symmetric and presented superimposable results). The volar forearm appears to be the area least influenced by the patients’age and presents the lowest correlation coefficients with other skin sites.
    Skin Research and Technology 10/2006; 4(2):83 - 87. DOI:10.1111/j.1600-0846.1998.tb00091.x · 1.31 Impact Factor
  • A Morrone · G Sacerdoti · G Franco · R Corretti · M Fazio ·
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    ABSTRACT: No abstract is available for this article.
    Clinical and Experimental Dermatology 08/2005; 30(4):447-8. DOI:10.1111/j.1365-2230.2005.01801.x · 1.09 Impact Factor
  • K Peris · S Chimenti · G Sacerdoti · L Muscardin · M Fazio ·

    Dermatology 02/1999; 199(2):192-4. DOI:10.1159/000018242 · 1.57 Impact Factor
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    ABSTRACT: Endothelins (ETs), in addition to their systematical activities, exert important functions at the skin level, such as increase of keratinocyte proliferation, neo-angiogenesis and leukocyte chemotaxis, which are among the main characteristics of psoriasis. To assess a possible ET-1 involvement in plaque-type psoriasis, ET-1 determinations were carried out in 15 sera and 8 lesional and non-lesional biopsy skin extracts from psoriatic patients and in 15 sera and 5 biopsy skin extracts from healthy volunteers, sex- and age-matched, using commercially available ELISA kits. A statistical analysis of the results showed that ET-1 levels were increased in sera of psoriatic patients, as compared to normal subjects (p = 0.04). In addition, there was a significant correlation between both serum (r = 0.60, p = 0.02) and lesional skin (r = 0.80, p = 0.03) ET-1 values versus the Psoriasis Area and Severity Index scores. Significant increases of the lesional versus the non-lesional (p = 0.01) and versus the normal (p = 0.04) ET-1 skin extract values were observed, together with a significant correlation between lesional and non-lesional ET-1 skin levels (r = 0.79, p = 0.03). These findings were also confirmed at the mRNA level, using RT-PCR analysis, where increased ET-1 mRNA levels, densitometrically measured, were found in the lesional samples versus non-lesional and normal skin. Since interleukin-8 is involved in psoriasis and shares some biological properties with ET-1, we further evaluated the levels of this cytokine in skin extracts. The behaviour of interleukin-8 paralleled that of ET-1, and a significant correlation between these two molecules was observed in the lesional skin (r = 0.76, p = 0.05). Taken together, these data stress that, as previously described for interleukin-8, ET-1 may be involved in inflammatory processes associated with psoriasis.
    Acta Dermato Venereologica 01/1998; 78(1):22-6. DOI:10.1080/00015559850135779 · 3.03 Impact Factor
  • L Balus · A Amantea · P Donati · M Fazio · M C Giuliano · M Bellocci ·
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    ABSTRACT: The clinical and histological features of the entities known as 'white fibrous papulosis of the neck' (WFPN) and 'acquired elastolysis of the papillary dermis simulating pseudoxanthoma elasticum' (PDE) are not clearly defined. This study was conducted to compare our experience of WFPN/PDE with those described in the literature. Twenty patients presented at our institution with papular eruptions involving the neck. The asymptomatic lesions, which ranged in colour from normal skin tones to yellowish, were isolated or coalescent. Microscopically, the papules showed elastolysis and fibrosis of the upper reticular and papillary dermis. A review of the literature shows similar characteristics in cases reported as WFPN and PDE. This study indicates that WFPN and PDE are variants of a single disorder that can be more precisely defined as 'fibroelastolytic papulosis of the neck' and which appears to be a manifestation of intrinsic skin ageing.
    British Journal of Dermatology 10/1997; 137(3):461-6. DOI:10.1046/j.1365-2133.1997.18711957.x · 4.28 Impact Factor

    Journal of the European Academy of Dermatology and Venereology 09/1997; 9. DOI:10.1016/S0926-9959(97)89477-9 · 2.83 Impact Factor

  • Journal of the European Academy of Dermatology and Venereology 09/1997; 9. DOI:10.1016/S0926-9959(97)89564-5 · 2.83 Impact Factor
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    ABSTRACT: TNF-alpha is a pleiotropic cytokine possibly involved in the pathogenesis of psoriasis. Objective: to analyze the serum TNF-alpha levels in plaque-type psoriatic patients to evaluate the concentrations, correlation with the severity score and behaviour after therapy. The serum TNF-alpha levels of thirty-seven patients (25 females and 12 males; median age: 52.5 years, range 18-81: median PASI score: 11.4, range 3.5-42) and thirty healthy controls (21 females and 9 males, median age: 48.5 years, range 25-77) were compared after measurements obtained employing commercially available ELISA kits. The median serum TNF-alpha levels of the patients were significantly higher than those of controls (p = 0.004). 30/37 patients were followed over time at 2 and 4 weeks of treatment. Twenty one subjects were also observed after 6 weeks. After effective treatments, both the PASI scores and the cytokine levels were significantly and concomitantly reduced (p < 0.001). Significant correlations were found when the TNF-alpha values were plotted against the PASI scores both at the time of patient enrollment and at all the subsequent times (118 observations). A significant correlation was observed between circulating TNF-alpha and sE-selectin in agreement with a possible functional activity of the cytokine. However, no correlation was found between the cytokine levels and other 4 soluble membrane molecules. Our findings indicate that the molecule studied, although non specific for the disease considered, presents a behaviour paralleling that of the disease severity and therefore might have clinical usefulness, particularly in monitoring the therapeutic effects.
    Journal of biological regulators and homeostatic agents 07/1997; 11(3):115-8. · 2.04 Impact Factor
  • A Mussi · M Carducci · C Bonifati · M Fazio · F Ameglio ·

    Acta Dermato Venereologica 06/1997; 77(3):237-8. · 3.03 Impact Factor
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    ABSTRACT: Increased levels of several cytokines, mainly proinflammatory mediators, have been reported in psoriatic lesions. Little information, if any, is available concerning other cytokines, especially those initially studied as marrow differentiation agents. Using the experimental approach of measuring cytokines released by skin organ cultures. IL-11 and three other proinflammatory cytokines (IL-1 beta, IL-6 and IL-8) were determined using commercially available ELISA kits in supernatants of ten biopsies from lesional and nonlesional psoriatic skin areas and in supernatants of biopsies from ten normal volunteers. The results obtained showed that the amounts of IL-11 and the other three modulators were significantly increased in the material from the lesional areas (P < 0.01). The amounts of IL-11, which is known to have functional activity similar to the proinflammatory cytokines and to share a receptor component with IL-6, were also correlated with the disease severity index (R = 0.69, P = 0.04). In addition, a nearly significant correlation was noted between the amounts of IL-11 released by the lesional and the nonlesional skin biopsies (R = 0.66, P = 0.05). More detailed studies are needed to clarify whether IL-11 plays a specific functional role in psoriasis, but this study emphasizes the complexity of the pathogenesis of psoriasis and the cytokine network, including activation of proinflammatory and haemopoietic biological response modifiers, in this disease.
    Archives for Dermatological Research 06/1997; 289(7):399-403. DOI:10.1007/s004030050211 · 1.90 Impact Factor
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    ABSTRACT: Several cytokines have been shown to be increased in psoriasis, mainly at the local and sometimes at the systemic level. At present, no data concerning the relationships between psoriasis and interleukin-7 (IL-7) are available. This biological modifier regulates immune response by means of its pleomorphic activities, including the ability to stimulate different monocyte functions, such as killing of intracellular pathogens, induction of cytokines, and enhancement of some membrane molecule expression. Study groups consisted of nine psoriatic and nine normal subjects. Using a commercially available immune-enzyme method, IL-7 concentrations were determined in various samples: biopsy and scale extracts, peripheral blood mononuclear cells (PBMCs) supernatants, and sera. The results show that IL-7 levels are significantly increased both in biopsy and in scale extracts obtained from lesional skin compared to those obtained from nonlesional and normal skin (P at least < 0.01). In addition, the serum values were higher in psoriatic patients than in the controls (P = 0.003). In contrast, no significant differences were observed in the supernatants of unstimulated PBMCs maintained in culture for 48 hr. These data suggest that IL-7 is involved in some way in the pathomechanisms of psoriasis and that the keratinocyte may be a candidate for psoriatic IL-7 overproduction.
    Clinical Immunology and Immunopathology 05/1997; 83(1):41-4. DOI:10.1006/clin.1996.4313
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    ABSTRACT: Corneometry has been considered useful both to evaluate disease severity and to monitor psoriatic patients during treatment. However, a limitation of this technique is that the patient's age influences the corneometric determinations, thus reducing their clinical usefulness. The aim of this study was, therefore, to establish whether age normalization of the corneometric results may provide more reliable data for clinical use. Corneometric levels were determined in 10 plaque-type psoriatic patients, under standard conditions. Eight serum variables, including transforming growth factor-beta 1 and seven soluble membrane molecules, were assayed with commercially available immune-enzyme methods in the same patients, whose age and PASI scores were also recorded. The age normalization procedure improved all the correlation coefficients calculated on the lesional or non-lesional corneometric values versus the PASI scores as well as versus the other serum variables. This approach may render corneometric determinations more useful to evaluate disease status or treatment effect in patient groups with plaque-type psoriasis.
    Acta Dermato Venereologica 04/1997; 77(2):110-4. · 3.03 Impact Factor
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    ABSTRACT: The surface area and micropore distribution of the porous glass prepared from borosilicate glass were controlled by the addition of metal oxides. When alumina or zirconia was added to the borosilicate glass, the micropore size distribution was very sharp and the maximum of the distribution was located at 1–2 nm. The addition of manganese oxide or antimonial oxide did not produce a sharp micropore distribution in the porous glass. When the hydrogenation of benzenes, substituted with a different number of methyl groups, was carried out over nickel catalysts supported on the porous glass prepared from the borosilicate glass with zirconia, the hydrogenation rate decreased according to the number of methyl groups and the position of the groups. These results indicate that a shape-selective nickel catalyst can be prepared from the porous glass synthesized from the borosilicate glass with a small amount of metal oxide.
    Microporous Materials 06/1996; 6(4). DOI:10.1016/0927-6513(95)00099-2
  • G. Sacerdoti · A. Amantea · P. Donati · G. Menaguale · M. Fazio ·
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    ABSTRACT: The authors present a case of a woman with Dowling-Degos disease in which the reticular pigmentation clearly involved the perianal and vulvar area. The extreme rarity of this localization and the importance of recognizing this disease in the mucosa is emphasized to permit identifying forms with few cutaneous characteristics and to distinguish them from other dermatoses.
    Journal of the European Academy of Dermatology and Venereology 12/1995; 6(1):62 - 64. DOI:10.1111/j.1468-3083.1996.tb00136.x · 2.83 Impact Factor
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    ABSTRACT: Increased tumour necrosis factor alpha has been found in psoriatic skin. This cytokine activates endothelial cells and induces the membrane E-selectin molecule (E-selectin or endothelial leucocyte adhesion molecule 1); the same cytokine is able to induce its own receptors. Since the soluble forms of E-selectin and tumour necrosis factor receptor (TNF-R, 60 kD) may be reliably measured in body fluids, these determinations have been performed in the sera of psoriatic patients. To evaluate endothelial activation in psoriatic patients, sE-selectin has been determined in patient sera and compared with those of a control group. sTNF-R (60 kD) was also measured in the same samples. Two commercially available enzyme immunoassay methods have been used to determine sE-selectin and sTNF-R (60 kD) in the sera of 19 patients with plaque-type psoriasis; 22 healthy subjects were used as controls. Significantly increased amounts of sE-selectin serum levels were found in psoriatic patients as compared to healthy controls. Moreover, a direct correlation between sE-selectin and PASI scores was observed. On the contrary, sTNF-R (60 kD) serum levels presented no increases. These data suggest that sE-selectin serum levels are a reliable marker of disease activity in psoriatic patients.
    Dermatology 02/1995; 190(2):128-31. DOI:10.1159/000246661 · 1.57 Impact Factor
  • M Fazio · S Lisi · A Amantea · A Maini · G Menaguale · G Sacerdoti · L Balus ·
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    ABSTRACT: Hereditary sclerosing poikiloderma is a genodermatosis with dominant autosomal transmission and variable penetration. The first case was described by Weary in 1969 in 7 members of two black families. A 10-year-old girl had localized regional poikiloderma of the fingers and club toes. These lesions were associated secondarily with linear symmetric bands of sclerotic tissue in the axiallary regions. On the X-ray examinations of the distal phalanges of the fingers and the toes showed a proximal growth foyer and absent ungueal phalanges, excepting in the fourth finger of the left hand. Capillaroscopy of the supra-ungueal fold of the fingers showed abnormal capillary circulation. Histology and ultrastructural examinations did not reveal any pathognomonic alterations. This case is the first reported in a white patient. The radiological aspect and the results of the capillaroscopy of the fingers and the toes have not been reported previously in this rare genodermatosis. Inheritance of this genodermatosis is poorly defined.
    Annales de Dermatologie et de Vénéréologie 02/1995; 122(9):618-20. · 0.92 Impact Factor
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    ABSTRACT: Tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and granulocyte monocyte-colony stimulating factor (GM-CSF) were measured in serum and involved and uninvolved skin blister fluids of 20 psoriatic patients and 10 healthy subjects, by enzyme immunoassay. TNF-alpha and IL-6 were always detectable in involved skin blister fluids, while GM-CSF was detected only in 45% of these samples. TNF-alpha, IL-6 and GM-CSF were detected in 95, 100 and 10% of uninvolved skin blister fluid samples, respectively. TNF-alpha and IL-6 were found in 50 and 30% of control blister fluids, while GM-CSF was never detected. In serum, TNF-alpha was detected in 75% of patients and in 70% of controls; IL-6 in 45% of patients and in no controls; and GM-CSF in 35% of patients and in 20% of the controls. The median TNF-alpha and IL-6 levels in involved skin were statistically higher than those of both uninvolved and control skin blister fluids. TNF-alpha and IL-6 levels in blister fluids obtained from both involved and uninvolved skin were higher than those of the patients' sera. GM-CSF, when present in involved skin blister fluids, showed correlated levels with the other cytokines (TNF-alpha: R = 0.85, P = 0.004; IL-6: R = 0.72, P = 0.03). TNF-alpha was highly correlated with IL-6 (R = 0.78, P < 0.00001) in involved skin blister fluids. TNF-alpha and IL-6 levels of involved skin blister fluids showed significant correlations with the psoriasis area and severity index scores in the patients, suggesting a direct relationship between these cytokines and the clinical manifestations of the disease. Moreover, the TNF-alpha levels were particularly related to the erythema scores in the patients, further supporting evidence of their role in the pathogenesis of the disease.
    Clinical and Experimental Dermatology 09/1994; 19(5):383-7. DOI:10.1111/j.1365-2230.1994.tb02687.x · 1.09 Impact Factor
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    ABSTRACT: Interleukin-1-beta (IL-1-beta), Interleukin-6 (IL-6) and Interferon-gamma (IFN-gamma) were measured by enzyme immunoassay (EIA) methods in blister fluids (BFs) obtained from both involved (ISBF) and non-involved skin (USBF) and in sera from 14 psoriatic patients. The same determinations were carried out in 14 sera and in 5 suction blister fluids from 14 normal subjects. IL-6 was always detectable in all skin fluids and in 3 psoriasis sera. IL-1-beta was measured only in 5 ISBFs and in 5 sera from the same patients. IFN-gamma was present in 11 ISBFs, in 5 USBFs and in 5 sera. The analysis of the levels found in the samples shows: 1) a local production of these cytokines, 2) the presence of detectable amounts of IL-6 and IFN-gamma in USBFs, and 3) a significant correlation between the IL-6 levels in the ISBFs and erythema score.
    Acta dermato-venereologica. Supplementum 02/1994; 186:23-4.
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    ABSTRACT: Sir, Interleukin-6 (IL-6), a pro-inflammatory cytokine [1, 2], has previously been detected in the sera of psoriatic patients [3, 4]. The importance of this cytokine in the pathogenesis of this disease has been based on the very high levels of IL-6 found in the lesions [3-5]. The improved sensitivity of the new enzyme immunoassays, commercially available in the last few years, has allowed the measurement of serum IL-6 levels of most psoriasis patients. This report presents six females with plaque-type psoriasis (median age 70 years, range 24-82 years) who were evaluated by a clinical index of disease severity: the psoriasis area severity index (PASI) [6], with scores ranging between 9.3 and 19.3 (median 11.6). The patients (previously untreated for at least 10 days) received daily UVB radiation plus ointment containing betamethasone dipropionate (0.064) and salicylic acid (3%) for at least 4 weeks. Serum IL-6 was measured with the IL-6 EASIA kit (Medgenix, Brussels, Belgium), with a 3 pg/ml sensitivity. Blood samples were taken before treatment and every 1-2 weeks thereafter. Statistical analysis was performed using non-parametric tests: Friedman variance analysis or Wilcoxon test to compare groups and Spearman's correlation test to evaluate the regression coefficients. The initial serum IL-6 levels ranged between 92 and 175 pg/ml (median 150 pg/ml). A significant correlation (r= 0.9, P
    International Journal of Clinical & Laboratory Research 02/1994; 24(2):122-3. DOI:10.1007/BF02593914
  • M Carducci · A Mussi · C Bonifati · M Fazio · F Ameglio ·

    Archives for Dermatological Research 02/1994; 286(7):420-2. · 1.90 Impact Factor