Masahiko Kawaguchi

Nara Medical University, Kashihara, Nara, Japan

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Publications (223)518.27 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Diabetes mellitus is associated with morbidity and progression of some cancers, such as hepatocellular carcinoma. It has been reported that sevoflurane, a volatile anesthetic agent commonly used in cancer surgery, can lead to lower overall survival rates than those observed when propofol is used to treat cancer patients, and sevoflurane increases cancer cell proliferation in in vitro studies. It has been also reported that glucose levels in rats anesthetized with sevoflurane were higher than those in rats anesthetized with propofol. We investigated the effect of sevoflurane, under conditions of high glucose and insulin, on cell proliferation in the human hepatocellular carcinoma cell line, HepG2. First, we exposed HepG2 cells to sevoflurane at 1 or 2 % concentration for 6 h in various glucose concentrations and then evaluated cell proliferation using the MTT assay. Subsequently, to mimic diabetic conditions observed during surgery, HepG2 cells were exposed to sevoflurane at 1 or 2 % concentration in high glucose concentrations at various concentrations of insulin for 6 h. One-percent sevoflurane exposure enhanced cell proliferation under conditions of high glucose, treated with 0.05 mg/l insulin. Our study implies that sevoflurane may affect cell proliferation in human hepatocellular carcinoma cells in a physiological situation mimicking that of diabetes.
    Journal of Anesthesia 05/2015; DOI:10.1007/s00540-015-2025-9 · 1.18 Impact Factor
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    ABSTRACT: Background: Sevoflurane is one of the commonly used volatile anesthetics in cancer patients. The protective effect of sevoflurane preconditioning has raised concerns about whether sevoflurane could act advantageously for survival even of cancer cells. Therefore, we investigated the effects of sevoflurane on proliferation in colon cancer cell lines. Methods: HCT116 and HT29 cells were plated in 96-well plates at a density of 1 × 104cells/well and incubated overnight On the next day, cells were exposed to 1% or 2% sevoflurane for 6 hr. After 24 hr recovery, we performed MTT assay. The absorbance of the formazan product was measured at a wavelength of 570 nm using 650 nm as the reference. In addition, to investigate the role of adenosine triphosphate-sensitive potassium (KAtp) channels, we conducted the same experiment under co-administration of KATP inhibitor, glibenclamide. Results: Only 1% sevoflurane significantly enhanced cell proliferation compared to the control in HCT116 and HT29 cells. Enhanced proliferation by sevoflurane was completely blocked by co-administration with glibenclamide in HCT116 cells. Conclusions: We had shown that 1% sevoflurane for 6 hr potentially enhances cell proliferation via Katp channels in cancer cells.
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    ABSTRACT: The Postoperative Quality of Recovery Scale (PQRS) is a multi-domain tracking scale to assess recovery after surgery. The PQRS is used in seven countries and five languages; however, the Japanese version of the PQRS (PQRSj) has not been established. We therefore translated the PQRS into Japanese, and examined the feasibility, reliability and validity of the PQRSj. The time taken to complete the test was measured to assess feasibility. Cronbach's alpha was calculated to assess reliability. The Mini Mental State Examination (MMSE) and the Japanese version of the Quality of Recovery Scale 40 (QoR-40 J) were performed for comparison with the PQRSj (validity). Fifty-one patients were enrolled in the study. The mean completion time was 3.9 min for baseline (feasibility). Cronbach's alpha was between 0.40 and 0.94 in each domain (reliability). A relationship was shown between cognitive domain and MMSE at baseline (r = 0.65, P < 0.01); however, no relationship was found between the other domains and the MMSE and QoR-40 J. Ceiling effects were observed in 78 % of the questions. These results indicate that the PQRSj can be used to assess recovery after surgery, although it may be better to revise some of the questions to improve the validity of the PQRSj.
    Journal of Anesthesia 11/2014; 29(3). DOI:10.1007/s00540-014-1945-0 · 1.18 Impact Factor
  • Mitsuru Ida · Masahiko Kawaguchi
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    ABSTRACT: Postoperative cognitive dysfunction (POCD) is seen not only after cardiac surgery but also after non-cardiac surgery. POCD leads to decreased quality of life and increased mortality. Various reports describe the occurrence and risk factor of POCD in adopting different neuropsychological tests and different follow-up periods. POCD is highly associated with age or inflammation in the central nerve system. Anti-inflammatory strategies including dexmedetomidine, statin or minocycline in the perioperative period may reduce incidence of POCD.
    Masui. The Japanese journal of anesthesiology 11/2014; 63(11):1228-34.
  • Masahiko Kawaguchi · Yuu Tanaka · Hitoshi Furuya
    Journal of Anesthesia 10/2014; 29(4). DOI:10.1007/s00540-014-1930-7 · 1.18 Impact Factor
  • Junko Ushiroda · Satoki Inoue · Yu Tanaka · Masahiko Kawaguchi
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    ABSTRACT: General anesthesia in patients with comorbid conditions may affect their intermediate or long-term outcomes. In this study, we evaluated the effects of provision of anesthesia on mortality in critical patients with comorbid conditions by retrospectively investigating one-year mortality in patients with ASA physical status more than III who underwent minor surgery for relative indications and nonfatal reasons. Data were collected during the period between January 2006 and December 2011. Eligible patients were those with ASA physical status more than III who underwent minor surgery under general anesthesia for relative indications and nonfatal reasons. Preoperative clinical information was collected from the patient's clinical charts. Comorbidity was quantified using the Charlson comorbidity index. All the patients were evaluated for in-hospital mortality and were followed-up for mortality at one-year. During the study period, 14, 979 patients underwent general anesthesia. Thirty six patients satisfied the eligibility for enrollment. Charlson comorbidity index of the patients ranged from one to five. No patients died during their hospital-stay; however, 4 patients were lost to follow up. Therefore, one-year mortality rates for each Charlson index category were 0%. The postoperative one-year mortality in patients with ASA physical status more than III undergoing minor surgery under general anesthesia for relative indications and nonfatal reasons was expected to be considerably small regardless of the Charlson index category.
    Middle East journal of anaesthesiology 10/2014; 22(6):597-602.
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    ABSTRACT: Background Intraocular pressure(IOP) has been shown to increase during prone position spine surgery. The present study was conducted to evaluate IOP changes and predictive factors associated with increased IOP during prone position spine surgery. Methods After institutional approval and written informed consent, 56 patients undergoing prone position spine surgery were studied. Anesthesia was maintained with propofol or sevoflurane, remifentanil, and fentanyl. IOP was measured using a Tono-Pen XL hand-held tonometer 10 min after induction of anesthesia, every 60 min after prone positioning, and 10 min after returning to the supine position. According to maximum IOP, patients were divided into group H with a maximum IOP value of ≥30 mmHg or group L with a maximum IOP value of
    Journal of Anesthesia 09/2014; 29(2). DOI:10.1007/s00540-014-1921-8 · 1.18 Impact Factor
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    ABSTRACT: Background Ki-67, cytokeratin 13, and/or cytokeratin 17 detection by immunohistochemistry has been reported to be useful for the diagnosis of oral precancerous lesions. However, the use of these markers remains controversial because of the lack of appropriately designed statistical studies. We assessed the hypothesis that Ki-67, cytokeratin 13, or cytokeratin 17 immunohistochemistry could facilitate the diagnosis of oral precancerous lesions and/or predict prognosis.Methods Epithelial dysplasia was classified as low grade (none or mild dysplasia) or high grade (moderate dysplasia, severe dysplasia, or carcinoma in situ). This study included 58 low-grade and 36 high-grade dysplasia cases. We used logistic regression to assess the diagnostic values of Ki-67, cytokeratin 13, and cytokeratin 17 for high-grade dysplasia. Correlations between these markers and the prognosis of oral atypical epithelium were assessed using the Cox proportional hazards model.ResultsKi-67 overexpression and cytokeratin 13 loss were independent diagnostic markers for high-grade dysplasia (odds ratios, 1.92 and 2.53; 95% confidence intervals, 1.03–3.58, and 1.19–5.38, respectively). The area under the curve of Ki-67 was 0.73 and that of cytokeratin 13 was 0.72. However, the combination of Ki-67 and cytokeratin 13 yielded the area under the curve of 0.78. Ki-67 overexpression was significantly associated with recurrence and/or malignant transformation of oral atypical epithelium (hazard ratio, 7.25; 95% confidence interval, 1.07–48.92).Conclusions Ki-67 overexpression and cytokeratin 13 loss may be useful for distinguishing oral precancerous lesions from reactive atypical epithelium. Moreover, Ki-67 overexpression may be a risk factor for recurrence and/or malignant transformation of oral atypical epithelium.
    Journal of Oral Pathology and Medicine 09/2014; 44(7). DOI:10.1111/jop.12262 · 1.93 Impact Factor
  • J. Ushiroda · S. Inoue · T. Kirita · M. Kawaguchi
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    ABSTRACT: Changes in airway dimensions can occur during general anaesthesia and surgery for a variety of reasons. This study explored factors associated with postoperative changes in airway dimensions. Patient airway volume was measured by acoustic reflectometory and neck muscle diameter by ultrasound echography in the pre- and post-anaesthetic periods in a total of 281 patients. Neck circumference was also assessed during these periods. A significant decrease in median (IQR [range]) total airway volume (from 63.8 (51.8–75.7 [14.7–103]) ml to 45.9 (33.5–57.2 [6.4–96.3]) ml, p < 0.0001), and a significant increase in muscle diameter (from 4.3 (3.3–5.6 [2.2–9.0]) mm to 5.8 (4.7–7.3 [2.8–1.3]) mm, p < 0.0001) and neck circumference (from 34.0 (32.5–37.0 [29.5–49.0]) cm to 35.0 (33.5–38.0 [30.5–50.5]) cm, p < 0.0001) were observed. It may be possible that changes in airway volume and neck circumference were influenced by surgical duration or peri-operative fluid management (ρ) = −0.31 (95% CI −0.24 to −0.01), p = 0.0301, −0.17 (−0.23 to −0.06), p = 0.0038, 0.23 (0.12–0.34), p < 0.0001, and 0.16 (0.05–0.27), p = 0.0062, respectively). The intra-oral space can significantly decrease and neck thickness increase after general anaesthesia, and might increase the risk of difficult laryngoscopy and intubation if airway management is required after extubation following general anaesthesia.
    Anaesthesia 08/2014; 69(12). DOI:10.1111/anae.12825 · 3.38 Impact Factor
  • Satoki Inoue · Ryuichi Abe · Yu Tanaka · Masahiko Kawaguchi
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    ABSTRACT: An urban legend that "you will get hurt if you go to hospital at the beginning of the fiscal year" is in circulation, because people in general suppose that inexperienced newcomers start to work at clinical practice during that time period. We tried to determine whether this urban legend was true or not by using data from our operation management system. We retrospectively conducted a study to investigate whether the number of cannulation failures, which was used as an index of patient disadvantages at clinical practice, could be affected by the volume of residents in clinical participation. The number of insertion trials per case was not prominent in the first month of the fiscal year. However, the number of insertion trials per case increased in proportion to the average number of residents per day. It seems that there was no evidence to support the urban legend that "you will get hurt if you go to hospital at the beginning of the fiscal year." However, our results suggest that rather than an urban legend, we are now confronting the fact that patients may suffer from medical disadvantages in the teaching hospitals.
    Journal of Anesthesia 07/2014; 29(1). DOI:10.1007/s00540-014-1867-x · 1.18 Impact Factor
  • The FASEB Journal 04/2014; 28(1). · 5.04 Impact Factor
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    ABSTRACT: Postoperative sore throat should be recognized seriously as an avoidable complication because of high incidence and dissatisfaction with anesthesia. The aim of the study was to identify the risk factors of postoperative sore throats in patients undergoing general anesthesia. We retrospectively studied 1,733 patients (above 18 years of age) who had undergone general anesthesia from January to December 2010 and who had visited Department of Anesthesia, postoperative anesthesia clinic. Age, ASA classification, position, airway device, methods of postoperative pain management and site of surgery were significantly identified as the associated factors for postoperative sore throat using univariate analysis. Under 65 year of age (odds ratio 2.3), operation of head and neck and pharynx (odds ratio 1.6), spine surgeries (odds ratio 0.33), laryngeal mask (odds ratio 0.47), and postoperative intravenous patient-controlled analgesia (IV-PCA, odds ratio 0.4) were significantly identified as the factors with multivariate studies. Six associated factors for postoperative sore throat were identified in this study.
    Masui. The Japanese journal of anesthesiology 04/2014; 63(4):401-5.
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    ABSTRACT: It has been reported that multiple puncture more than or equal to 3 times is a factor associated with the development of CVC-related complications. The present retrospective study was undertaken to evaluate the predictors and risks of multiple puncture during the insertion of CVC. After the IRB approval, 1296 patients who underwent CVC in the operating room were enrolled. Data were collected using CVC report and the medical charts. Multiple puncture was defined as the puncture performed more than or equal to three times during the insertion of CVC. Univariate and multivariate logistic regression analysis were performed to assess the predictors of multiple puncture during the CVC insertion. Univariate analysis revealed that age less than 6 years, complications during insertion and the way to use ultrasound echo were associated with multiple puncture. Multivariate analysis also revealed that age less than 6 years was a significant predictor for multiple puncture (odds ratio 2.08, 95% CI 1.01-4.29). The results of the study indicate that the age less than 6 years is a significant predictor for multiple puncture during the CVC insertion.
    Masui. The Japanese journal of anesthesiology 02/2014; 63(1):62-7.
  • Yuu Tanaka · Atushi Yoshimura · Kyoko Tagawa · Dai Shida · Masahiko Kawaguchi
    Journal of Anesthesia 01/2014; 28(1). DOI:10.1007/s00540-013-1781-7 · 1.18 Impact Factor
  • THE JOURNAL OF JAPAN SOCIETY FOR CLINICAL ANESTHESIA 01/2014; 34(4):531-537. DOI:10.2199/jjsca.34.531
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    ABSTRACT: The purpose of the present study was to examine the effect of Daikenchuto (DKT) on postoperative intestinal function and nutritional status of patients.The subjects were 40 patients who had undergone immediate reconstruction with free tissue graft for the defect after radical surgery for advanced oral cancer from March 2008 to May 2013. Twenty patients were administered 15g DKT a day from postoperative day 1, while the other 20 patients served as controls without DKT administration. Intestinal function such as peristaltic sound, passage of flatus and bowel movement, start of enteral nutrition (EN), and amount and rate of body weight loss 2 weeks after surgery were evaluated. Peristaltic sound (p < 0.001), passage of flatus (p < 0.005), bowel movement (p < 0.005), and start of EN (p < 0.01) were observed earlier in the DKT group. The amount (p < 0.05) and rate (p < 0.05) of body weight loss were also smaller in the DKT group. Preoperative chemoradiotherapy or surgical stress did not affect these indexes. These results suggest that DKT is effective for the improvement of intestinal function and weight maintenance after reconstruction with free tissue graft in patients with advanced oral cancer.
    01/2014; 26(2):37-44. DOI:10.5843/jsot.26.37
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    ABSTRACT: Airway changes generally occur in normal gravidas; however, these changes could cause critical situations in specific populations. This article presents the case of a difficult airway patient that went into shock because of atonic bleeding after vaginal delivery for stillbirth. A 32-yr-old woman with atonic bleeding after vaginal delivery for stillbirth was transferred to our hospital. She manifested shock, and her respiratory condition was progressively deteriorating. Airway obstruction caused by neck swelling and pharyngolaryngeal edema was apparent. We tried tracheal intubation using direct and indirect laryngoscopes. However, it turned out that insertion of the laryngoscopic devices to the oral cavity was impossible. After several attempts using the Trachlight™, successful intubation was finally made. After hysterectomy, she was admitted to the intensive care unit (ICU) and treated for five days. At discharge from the ICU, her Mallampati score was I-II. Her body weight decreased 60kg to 51kg during ICU stay. We believe that concomitant attacks of labor and delivery and fluid resuscitation probably worsened upper airway and neck edema enough to cause acute airway obstruction and difficult laryngoscopy.
    12/2013; 63(6):508-10. DOI:10.1016/j.bjane.2012.09.005
  • Satoki Inoue · Yasumitsu Nomura · Masahiko Kawaguchi
    Journal of Anesthesia 11/2013; 28(3). DOI:10.1007/s00540-013-1742-1 · 1.18 Impact Factor
  • Junko Ushiroda · Satoki Inoue · Hitoshi Furuya · Masahiko Kawaguchi
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    ABSTRACT: We report a case of ventilation failure due to supraglottic air leakage with the use of uncuffed tracheotomy tube. A 4-year-old girl with 22q11.2 deletion syndrome after tracheotomy due to tracheomalacia developed left caudate bleeding and was admitted urgently. She required mechanical ventilation but suffered from a supraglottic air leakage which prevented adequate ventilation via a tracheostomy site. To stop the supraglottic air leakage, a size 1.5 laryngeal mask airway (LMA) was inserted into the oral pharynx and the cuff was inflated. The supraglottic air leakage was not detected under mandatory mechanical ventilation following seal of the connector of the LMA with a piece of tape. The respiratory condition of the patient improved gradually. The use of an LMA may be useful to stop or significantly decrease the air leak.
    Masui. The Japanese journal of anesthesiology 11/2013; 62(11):1360-1.
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    ABSTRACT: De manera general, las alteraciones en las vías aéreas se dan en las embrazadas normales; sin embargo, esas alteraciones pueden generan situaciones críticas en poblaciones específicas.
    11/2013; 63(6):508-510. DOI:10.1016/j.bjanes.2012.09.005

Publication Stats

2k Citations
518.27 Total Impact Points


  • 1996–2014
    • Nara Medical University
      • • Department of Anesthesiology
      • • Department of Orthopaedic Surgery
      • • Department of Pathology
      Kashihara, Nara, Japan
  • 2008–2009
    • National Defense Medical College
      • Department of Anesthesiology
      Tokorozawa, Saitama, Japan
  • 2006
    • Teikyo University
      Edo, Tōkyō, Japan
  • 2004
    • Mayo Clinic - Scottsdale
      Scottsdale, Arizona, United States
  • 1993–1994
    • National Cerebral and Cardiovascular Center
      • Department of Cardiovascular Medicine
      Ōsaka, Ōsaka, Japan