Lei Wang

China National Offshore Oil Corporation, Peping, Beijing, China

Are you Lei Wang?

Claim your profile

Publications (800)2083.2 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: In this work, a series of new metal phosphonates were hydrothermally synthesized and structurally characterized based on m-xylylenediphosphonic acids (H4L), including [M(H2L)(bpy)] (M = Mn 1, Co 2), [MH2L)(phen)] (M = Co 3, Cu 4), and [Cu2(H2L)2(bpy″)2] 5 (bpy = 2,2′-bipyridine, phen = 1,10-phenanthroline, bpy″ = 4,4′-dimethyl-2,2′-bipyridine). X-ray diffraction indicated that compounds 1 and 2 were isomorphic. Complexes 3 and 4 were shown to crystallize in different space groups but had similar crystallographic units. In the complexes, H4L was seen to partially deprotonate to afford H2L2−. The H2L2− ligands in 1–4 functioned as tetradentate ligands with each phosphonate group adopting bidentate coordination mode with two M centers to generate a 2D layer. In addition, the H2L2− anions in 5 functioned as tridentate ligands with one phosphonate group adopting the bidentate mode and another phosphonate group adopti ng the monodentate mode, thus linking three Cu atoms to provide a 1D chain. The IR and thermal stabilities of these compounds were subsequently examined.
    Chinese Science Bulletin 12/2014; 59(34). · 1.37 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Gastric cancer (GC) is one of the most threatening diseases. The symptoms of GC are complex and hard to detect, which also contribute to the poor prognosis of GC. Besides, the current diagnosis for GC is expensive and invasive. Thus, a fast, noninvasive biomarker is urgently needed for GC screening. MicroRNAs (miRNAs) are small noncoding RNAs, which are involved in a great variety of pathological processes, particularly carcinogenesis. MiRNAs are stable in gastric juice, plasma as well as serum, which facilitate it to be a promising biomarker for cancer. In this study, we selected three novel miRNAs, i.e., miR-233, miR-16, and miR-100, to investigate their potential diagnostic value in GC screening. A total of 50 GC patients and 47 healthy controls were involved in this study. Blood serum samples were collected; RNAs were extracted and normalized with U6 snRNA as the internal control; qRT-PCR was performed for relative expression of target miRNAs. Levels of miRNAs expression were compared by Student's t test for the comparison between two groups, and one-way ANOVA was used for multiple comparisons. The expression of miR-223, miR-16, and miR-100 was all significantly higher in GC patients than controls (all P < 0.001). All the tested miRNAs were manifested to be valuable biomarkers for GC. Relative expression of these miRNAs was significantly correlated with clinical characteristics of GC patients, such as TNM stage (P = 0.036 for miR-223; P < 0.001 for miR-100), metastatic status (P = 0.045 for miR-223; P = 0.031 for miR-16; P = 0.006 for miR-100), tumor size (P = 0.042 for miR-223; P = 0.031 for miR-16; P < 0.001 for miR-100), and differentiation grade (P = 0.036 for miR-223; P = 0.030 for miR-16; P = 0.034 for miR-100). However, in T classification, which considered both tumor size and direct extent of primary tumor, the difference in target miRNAs expression was not significant. In summary, we confirmed the diagnostic value of serum miR-223, miR-16, and miR-100 in GC. Significantly elevated expression of the three miRNAs was also observed in advanced GC patients, which suggested their availability in cancer staging.
    Medical oncology (Northwood, London, England). 12/2014; 31(12):298.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Our results showed that 70.6% and 65.7% of the colorectal cancer tissues had positive HLA-G or HLA-E expression.•Cox multivariate analysis showed that only HLA-G expression can serve as independent factor for OS.•Our results also showed that the expression of HLA-E was significantly correlated with tumor metastasis.
    Cellular Immunology. 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: Solvothermal reactions of tetrabromoterephthalic (H2tbtpa) acid and different bis-imidazoles with cadmium nitrate provided four new Cd(II) coordination polymers (CPs), namely, {[Cd(bip)(tbtpa)]•H2O}n (1), {[Cd2(bibp)(tbtpa)2(H2O)4]•H2O}n (2), {[Cd(1,2-mbix)(tbtpa)]•H2O}n (3) [Cd(1,2-mbix)(tbtpa)]n (4), (bip = 1,4-di(1H-imidazol-1-yl)benzene, bibp = 1,1'-(2,5-dimethyl-1,4-phenylene)bis(1H-imidazole), 1,2-mbix = 1,2-bis((2-methyl-1H-imidazol-1-yl)methyl)benzene, H2tbtpa = tetrabromoterephthalic acid). All of the compounds have been structurally characterized by single-crystal X-ray diffraction analyses and further characterized by elemental analyses, IR spectroscopy, powder X-ray diffraction (PXRD), and thermogravimetric analyses (TGA). Single crystal X-ray diffraction analysis reveals that compound 1 exhibits a 3D diamond-type (dia, 66) framework of 3-fold interpenetration. Compound 2 displays a noninterpenetrated 3D network with the classical pcu topology. Compound 3 exhibits a polyrotaxane-like 2D + 2D → 2D layer with 44-sql topology and the 2D sheets were further formed a 3D framework by π•••π interaction. Compound 4 is a 2D 44-sql network and the adjacent 2D networks are packed parallel in a •••ABAB••• fashion. Moreover, the thermal stability and photoluminescence properties of the compounds were investigated.
    RSC Advances 10/2014; · 3.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: IntroductionThe onset of distal metastasis, which underlies the high mortality of breast cancers, warrants substantial studies to depict its molecular basis. Nuclear factor of activated T cells 5 (NFAT5) is upregulated in various malignancies and is critically involved in migration and invasion of neoplastic cells. Nevertheless, the metastasis-related events potentiated by this transcriptional factor and the mechanism responsible for NFAT5 elevation in carcinoma cells remain to be fully elucidated.Methods The correlation of NFAT5 with breast cancer invasiveness was investigated in vitro and clinically. The genes transcriptionally activated by NFAT5 were probed and their roles in breast cancer progression were dissected. The upstream regulators of NFAT5 were studied with particular attempt to explore the involvement of non-coding RNAs, and the mechanism underlying the maintenance of NFAT5 expression was deciphered.ResultsIn metastatic breast cancers, NFAT5 promotes epithelial-mesenchymal transition (EMT) and invasion of cells by switching on the expression of the calcium binding protein S100A4, and facilitates the angiogenesis of breast epithelial cells and thus the development of metastases by transcriptionally activating vascular endothelial growth factor C (VEGF-C). NFAT5 is directly targeted by miR-568, which is in turn suppressed by the long non-coding RNA, Hotair, via a documented in trans gene silencing pattern, that is recruitment of the polycomb complex (Polycomb Repressive Complex 2; PRC2) and LSD1, and consequently methylation of histone H3K27 and demethylation of H3K4 on the miR-568 loci.Conclusion This study unravels a detailed role of NFAT5 in mediating metastatic signaling, and provides broad insights into the involvement of Hotair, in particular, by transcriptionally regulating the expression of microRNA(s), in the metastasis of breast cancers.
    Breast cancer research: BCR 10/2014; 16(5):454. · 5.87 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The determination of trace analytes based on membrane filtration–enrichment and diffuse reflectance spectroscopic technique has gained increasing concern in the past decade due to its simplicity, rapidity and high sensitivity. However, poor repeatability primarily attributed to the distinction between membrane filters limits the development of this technique. In the current study, a simple and effective multichannel device is specially designed for the membrane filtration–enrichment process. The device is able to enrich six samples simultaneously on different positions of a membrane filter and allows to fulfil the spectroscopic measurement of six samples with only one membrane filter. The proposed approach avoided the effects caused by the nonuniform membrane filters on the performance of the enrichment process. Accuracy and repeatability have been improved significantly for the subsequent on-line spectroscopic detection. A case study was carried out to assess this method utilizing the carcinogenic dye rhodamine B (RhB) as a model analyte. Under the optimal conditions, linearity of calibration curve based on the Kubelka-Munk function was achieved in the concentration range of 2 – 30 μg L-1 with the correlation coefficient (R2) of 0.9924. Good repeatability was achieved with three average relative standard deviation (RSD) values of 3.6%, 3.8% and 3.8% corresponding to the solutions of 30, 10 and 5 μg L-1 RhB, respectively. The presented method was successfully employed to quantify RhB in soft drink and river water samples.
    RSC Advances 10/2014; · 3.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new protocol for Ru-catalyzed decarboxylative cyclization of mandelic acids with acrylates was firstly established that allows the efficient construction of phthalide skeleton. Interestingly, this reaction underwent a decarboxylative process, in which only divinylation was observed and the subsequent cyclization led to the formation of phthalides.
    Chemical Communications 10/2014; · 6.38 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Ubiquitin ligase Nrdp1 (neuregulin receptor degradation protein 1) plays important roles in multiple physiological process because it can ubiquitinate various substrates such as ErbB3, BRUCE, MyD88, C/EBPβ, and Parkin, and so forth. In addition to the physiological function, it was also found to be involved in tumor progression. It has been shown that loss of Nrdp1 enhances breast cancer cell growth. Up to now, the role of Nrdp1 in glioma has not been elucidated. Here, we reported that Nrdp1 as well as cleaved caspase 3 was lower expressed in human glioma tissues comparing with the nontumorous. And then we found that the expression of Nrdp1 and cleaved caspase 3 was increased in the treatment of Temozolomide (TMZ), a drug for glioma chemotherapy. Further investigation indicated that transient transfection of Nrdp1 significantly promoted cell apoptosis by aggravating the degradation of BRUCE and activation of caspase 3. In addition, overexpression of Nrdp1 augmented TMZ induced apoptosis by evaluating the degradation of BRUCE and the activation of caspase 3, while silencing of Nrdp1 reduced the sensitivity to the TMZ by inhibiting the degradation of BRUCE and the activation of caspase 3 in human glioma cells. These observations show that Nrdp1 is a pro-apoptotic protein in human glioma and lower expression of Nrdp1 in human glioma may promote tumor progression by reducing apoptosis, suggesting that Nrdp1 may be an important regulator in the development of human glioma. © 2014 IUBMB Life, 2014
    International Union of Biochemistry and Molecular Biology Life 10/2014; · 2.79 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Left-sided malignant colonic obstruction is one of the most difficult clinical problems; however, no studies compared the two most common used surgical approach laparoscopic and open colorectomy till now. The purpose of this study was to investigate the short- and long-term outcomes of laparoscopy and open colorectomy for left-sided malignant colonic obstruction. A total of 193 colorectal carcinoma patients (55 patients who underwent laparoscopic colorectomy and 138 who underwent open colorectomy) with left-sided colonic obstruction and surgical therapy, between May 2007 and March 2012, are included in the study. The short-term and long-term outcomes including curative resection rate, hospital stay time, complications, 1-, 3- and 5-year survival rates and recurrence rate, as well as recurrence-free survival rate were analyzed retrospectively. No significant difference was found between the laparoscopic and open groups about the short-term outcomes, such as the curative resection rate (81.82 vs. 78.99 %, P = 0.658), hospital stay time (24.22 ± 17.09 vs. 24.19 ± 14.76 day, P = 0.990), the overall and respective complications (32.73 vs. 39.63 %, P = 0.674). Long-term outcomes, including 1-, 3- and 5-year survival rates (P = 0.518), recurrence rates (P = 0.320), and recurrence-free survival rates (P = 0.988), were also indicated no significant differences between the two patient groups. Laparoscopy might not have advantages on left-sided malignant colonic obstruction compared with open colorectal resection in both short-term and long-term outcomes.
    Medical oncology (Northwood, London, England). 10/2014; 31(10):213.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Spermatogonial stem cells (SSCs) are undifferentiated cells that are required to maintain spermatogenesis throughout the reproductive life of mammals. Although SSC transplantation and culture provide a powerful tool to identify the mechanisms regulating SSC function, the precise signalling mechanisms governing SSC self-renewal and specific surface markers for purifying SSCs remain to be clearly determined. In the present study, we established a steady SSC culture according to the method described by Shinohara's lab. Fertile progeny was produced after transplantation of cultured SSCs into infertile mouse testis, and the red fluorescence exhibited by the culture cell membranes was stably and continuously transmitted to the offspring. Next, via advanced mass spectrometry and an optimized proteomics platform, we constructed the proteome profile, with 682 proteins expressed in SSCs. Furthermore bioinformatics analysis showed that the list contained several known molecules that are regulated in SSCs. Several nucleoproteins and membrane proteins were chosen for further exploration using immunofluorescence and RT-PCR. The results showed that SALL1, EZH2, and RCOR2 are possibly involved in the self-renewal mechanism of SSCs. Furthermore, the results of tissue-specific expression analysis showed that Gpat2 and Pld6 were uniquely and highly expressed in mouse testes and cultured SSCs. The cellular localization of PLD6 was further explored and the results showed it was primarily expressed in the spermatogonial membrane of mouse testes and cultured SSCs. The proteins identified in this study form the basis for further exploring the molecular mechanism of self-renewal in SSCs and for identifying specific surface markers of SSCs.
    Journal of Cellular and Molecular Medicine 10/2014; · 4.75 Impact Factor
  • ChemInform 09/2014; 45(40).
  • [Show abstract] [Hide abstract]
    ABSTRACT: The nerve growth factor receptor (NGFR/p75) is a potential tumor suppressor, but its role in colorectal cancer (CRC) is unknown. Here, the hypermethylation status, biological function, and clinical relevance were determined for p75NGFR in CRC. The methylation status and expression of p75NGFR were assessed in CRC cell lines and clinical tissues by bisulfate genomic sequencing (BGS), qRT-PCR and immunoblot assay. Methylation of p75NGFR was frequently found in CRC, leading to its silencing or down-regulation, and it was effectively restored by a demethylation agent. The overexpression of p75NGFR in multiple CRC cell model systems significantly inhibited cell proliferation (concomitant with G1-phase arrest), invasion, and colony formation and induced cell apoptosis. In contrast, p75NGFR knockdown significantly promoted proliferative and invasive phenotypes. Importantly, p75NGFR methylation was observed in the majority of primary CRC specimens and was associated with histological grade and preoperative serum CA19-9 levels. Multivariate analysis indicated that patients who lack p75NGFR have reduced overall survival (OS; 64% vs. 75%, p=0.028) and disease-free survival (DFS; 61% vs. 72%, p=0.034) compared with p75NGFR-positive patients. In conclusion, p75NGFR is predominantly silenced or down-regulated in CRC, and its biological activities are consistent with it being a relevant tumor suppressor. Implications: p75NGFR is a candidate tumor suppressor and has independent prognostic potential in CRC.
    Molecular cancer research : MCR. 09/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: The supramolecular reactions of tetrabromoterephthalic acid (H2-TBTA) with a series of N-heterocycles afford eight new complexes, namely, [(H2-BTAH)2·(TBTA)·(H2-TBTA)] (1), [(H2-Bim)2·(TBTA)·(H2-TBTA)·2H2O] (2), [(H-8-HQ)2·(TBTA)·3H2O] (3), [(5-NO2-phen)2·(H2-TBTA)] (4), [(4,6-DHP)2·(H2-TBTA)·2H2O] (5), [(H2-2,4-DMI)2·(TBTA)·(H2-TBTA)2] (6), [(H2-3,5-DMP)2·(TBTA)] (7), and [(H-4-CNpy)2·(TBTA)·(H2-TBTA)] (8) (H-BTAH = 1H-Benzotriazole, H-Bim = 1H-Benzimidazole, 8-HQ = 8-Hydroxyquinoline, 5-NO2-phen = 5-Nitro-1,10-phenanthroline, 4,6-DHP = 4,6-Dihydroxypyrimidine, H-2,4-DMI = 2,4-Dimethylimidazole, H-3,5-DMP = 3,5-Dimethylpyrazole, and 4-CNpy = 4-Cyanopyridine), which have been prepared under mild and identical reaction conditions in a mixture of distilled water and ethanol. All the complexes were fully characterized by single crystal X-ray diffraction analysis, elemental analysis, infrared spectroscopy (IR), and thermogravimetric analysis (TGA). Combining the various N-containing ligands and the diversity of the hydrogen bonds, the eight crystals display amusing structural characteristics. Of this, complex 3 forms three-dimensional (3D) network through the C–H···Br, whilst the O–H···Br consist in the 3D construction of compound 2. Complexes 4-8 generate a 3D supramolecular structure by large amounts of hydrogen bonds. In crystal 1, the π–π stacking interactions play an important part in the 3D network. The thermal stability of crystals 1-8 has been investigated by thermogravimetric analysis (TGA) of mass loss.
    RSC Advances 09/2014; · 3.71 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Epidermal growth factor receptor (EGFR), which is overexpressed in psoriatic lesions, has been proven to contribute to the hyperproliferation of keratinocytes in psoriasis. Single nucleotide polymorphisms (SNPs) involved in miRNAs that can regulate the expression of EGFR could potentially influence the development of psoriasis. The present study investigated the association between a functional SNP of rs2910164 in miR-146a and the risk of psoriasis in the Chinese Han population. A total of 521 Han Chinese patients with psoriasis and 582 healthy controls were recruited in this study. The miR-146a rs2910164 SNP was genotyped by polymerase chain reaction-restriction fragment length polymorphism. Overall, a significantly increased risk of psoriasis was associated with the rs2910164 miR-146a CG and GG genotypes (adjusted OR, 1.38; 95% CI, 1.06-1.80). Furthermore, the rs2910164G allele in miR-146a attenuated its inhibitory regulation on the expression of EGFR as well as the proliferation of human keratinocytes, and lowered the level of miR-146a in the psoriatic lesions. These findings indicate that the rs2910164G allele in miR-146a weakens its suppression on the proliferation of keratinocytes probably through the decreased inhibition of the target gene, EGFR, which may account for the increased risk of psoriasis in this study population.
    Journal of Cellular and Molecular Medicine 09/2014; · 4.75 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Although conscientiousness was commonly viewed as a type of personal resource to help individuals reduce strain or mitigate the impacts of stressors, empirical research demonstrated mixed results. Based on the personal resource allocation perspective, we posited that rather than functioning as personal resource per se, conscientiousness may act as a key factor influencing how individuals allocate their personal resources. The current study examined the moderating roles of conscientiousness in the relationships that work stressors (i.e., challenge stressors and hindrance stressors) have with employee psychological strain and job performance by using multi-source, time-lagged data collected from 250 employees working at two companies. The results showed that both challenge stressors and hindrance stressors were positively related to psychological strain. Conscientiousness moderated the relationships between both stressors and psychological strain, such that the positive relationships were stronger for individuals with high conscientiousness. Conscientiousness also moderated the relationship between challenge stressors and performance, such that the relationship was positive for individuals with high conscientiousness but negative for those with low conscientiousness. Altogether, the findings suggest that conscientiousness acts as a double-edged sword that both promotes performance and exacerbates the stress reaction of employees when they are confronted with stressful situations. Copyright © 2014 John Wiley & Sons, Ltd.
    Journal of Organizational Behavior 09/2014; · 3.85 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Big data benchmark suites must include a diversity of data and workloads to be useful in fairly evaluating big data systems and architectures. However, using truly comprehensive benchmarks poses great challenges for the architecture community. First, we need to thoroughly understand the behaviors of a variety of workloads. Second, our usual simulation-based research methods become prohibitively expensive for big data. As big data is an emerging field, more and more software stacks are being proposed to facilitate the development of big data applications, which aggravates hese challenges. In this paper, we first use Principle Component Analysis (PCA) to identify the most important characteristics from 45 metrics to characterize big data workloads from BigDataBench, a comprehensive big data benchmark suite. Second, we apply a clustering technique to the principle components obtained from the PCA to investigate the similarity among big data workloads, and we verify the importance of including different software stacks for big data benchmarking. Third, we select seven representative big data workloads by removing redundant ones and release the BigDataBench simulation version, which is publicly available from http://prof.ict.ac.cn/BigDataBench/simulatorversion/.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cyclic dinucleotides are a newly expanded class of second messengers that contribute to the regulation of multiple different pathways in bacterial, eukaryotic, and archaeal cells. The recently identified Vibrio cholerae dinucleotide cyclase (DncV, the gene product of VC0179) can generate three different cyclic dinucleotides and preferentially synthesize a hybrid cyclic-GMP-AMP. Here, we report the crystal structural and functional studies of DncV. We unexpectedly observed a 5-methyltetrahydrofolate diglutamate (5MTHFGLU2) molecule bound in a surface pocket opposite the nucleotide substrate-binding groove of DncV. Subsequent mutagenesis and functional studies showed that the enzymatic activity of DncV is regulated by folate-like molecules, suggesting the existence of a signaling pathway that links folate-like metabolism cofactors to the regulation of cyclic dinucleotide second messenger synthesis. Sequence analysis showed that the residues involved in 5MTHFGLU2 binding are highly conserved in DncV orthologs, implying the presence of this regulation mechanism in a wide variety of bacteria.
    Molecular Cell 09/2014; · 15.28 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: As a ligand-activated transcriptional factor, FXR has a variety of biological functions, such as biosynthesis of bile acids, metabolism of lipid and glucose homeostasis, and thus is related to multiple diseases, especially metabolic syndrome. In this study, to discover new FXR modulators, we have designed a strategy by combining 3D shape similarity search and structure-based docking methods. Taking two FXR ligands that we previously reported as the reference molecules, virtual screening was performed against the Enamine database and finally 59 compounds were selected for bioassay. Among them, four compounds exhibited agonistic or antagonistic activities against FXR in HTRF assay. Two of them were found to be new, potent FXR antagonists in cell-based assay with IC50 values of 8.39 and 6.53 μM, respectively.This article is protected by copyright. All rights reserved.
    Chemical Biology &amp Drug Design 09/2014; · 2.47 Impact Factor
  • Lei Wang, Tianwen Gao, Gang Wang
    [Show abstract] [Hide abstract]
    ABSTRACT: Background Verrucous hemangioma is a rare form of vascular malformation. Previous studies have reported positive expression of Wilms tumor 1 (WT-1) and Glut-1 and negative expression of lymphatic markers such as D2-40 and Prox1 in verrucous hemangioma cases. However, the sample sizes of these studies were usually small.Methods We analyzed 74 cases of verrucous hemangioma diagnosed in a single dermatology department and performed immunohistochemical analysis of vascular and lymphatic markers in all cases.ResultsVerrucous hemangioma was usually located on the extremities. Most lesions presented as solitary or multiple hyperkeratotic plaques or nodules with various diameters. Histopathologically, the lesions showed proliferation of small- to medium-sized vessels from the papillary dermis to subcutaneous tissue. The density of the proliferated vessels varied between cases. The vessels were positive for CD31 in 74 cases, focally positive for Prox1 in 63 cases, focally positive for D2-40 in 10 cases, negative for lymphatic vessel endothelial hyaluronan receptor-1 in 74 cases, negative for WT-1 in 60 cases, and positive for Glut-1 in 49 cases.Conclusion Verrucous hemangioma is a vascular malformation with an incomplete lymphatic immunophenotype, as indicated by positive staining for Prox1 and negative staining for WT-1 in the majority of instances.
    Journal of Cutaneous Pathology 09/2014; · 1.77 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the present study was to investigate the abnormal calcium re-uptake function of myocardium sarcoplasmic reticulum (SR) in rabbits with heart failure, as well as potential mechanisms. Heart failure model was established in rabbits through aortic insufficiency and constriction of abdominal aorta. The SR Ca(2+) re-uptake function was measured with a calcium imaging device. The activity of myocardium SR calcium adenodine triphosphatase 2a (SERCA2a) was measured by inorganic phosphate. The protein expressions of SERCA2a, CaMKII, PKA, PP1α, phospholamban (PLB), PLB-Ser(16) and PLB-Thr(17) were evaluated by Western blot. The activities of PKA and CaMKII were detected by γ-(32)P substrate incorporation. The results showed that, compared with the sham operation group, the heart failure group exhibited reduced Ca(2+) re-uptake amount (P < 0.01) and the expression and activity of SERCA2a (P < 0.05 or P < 0.01), decreased expression of PLB and its phosphorylation status in sites of Ser(16) and Thr(17) (P < 0.05), increased expressions and activities of PKA and CaMKII (P < 0.05 or P < 0.01), and increased expression of PP1α (P < 0.05). These results suggest that the abnormal Ca(2+) re-uptake function in heart failure is related with reduced expression and activities of SERCA2a, as well as reduced expression of PLB and its phosphorylation status. Both PLB-Ser(16) and -Thr(17) may be involved in the regulation of myocardium SR calcium pump activity in heart failure.
    Sheng li xue bao: [Acta physiologica Sinica] 08/2014; 66(4):483-8.

Publication Stats

6k Citations
2,083.20 Total Impact Points


  • 2014
    • China National Offshore Oil Corporation
      Peping, Beijing, China
    • University of Science and Technology of China
      • School of Life Sciences
      Luchow, Anhui Sheng, China
  • 2013–2014
    • Hebei Medical University
      Chentow, Hebei, China
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China
    • Southeast University (China)
      Nan-ching-hsü, Jiangxi Sheng, China
    • Zhengzhou University
      Cheng, Henan Sheng, China
    • 307 Hospital of the Chinese People's Liberation Army
      Peping, Beijing, China
    • Zhengzhou Tobacco Institute
      Chen-chu-shan, Jiangxi Sheng, China
    • Anhui Agricultural University (AHAU)
      Luchow, Anhui Sheng, China
    • Tianjin Medical University
      T’ien-ching-shih, Tianjin Shi, China
    • Liaoning Universtity of Traditional Chinese Medicine
      Feng-t’ien, Liaoning, China
    • Southern Medical University
      Shengcheng, Guangdong, China
    • Shenzhen Second People's Hospital
      Shen-ch’üan-shih, Zhejiang Sheng, China
  • 2011–2014
    • Dalian Institute of Chemical Physics
      Lü-ta-shih, Liaoning, China
    • Yangzhou University
      Chiang-tu, Jiangsu Sheng, China
    • Shenyang Ligong University
      Feng-t’ien, Liaoning, China
    • University Town of Shenzhen
      Shen-ch’üan-shih, Zhejiang Sheng, China
    • National Institute for the Control of Pharmaceutical and Biological Products
      Peping, Beijing, China
    • University of New South Wales
      • School of Biotechnology and Biomolecular Sciences (BABS)
      Kensington, New South Wales, Australia
    • Beijing Institute of Microbiology and Epidemiology
      Peping, Beijing, China
    • Tongji Medical University
      Shanghai, Shanghai Shi, China
    • Guangzhou University of Traditional Chinese Medicine
      Shengcheng, Guangdong, China
    • Anhui Medical University
      Luchow, Anhui Sheng, China
    • Northeastern University (Shenyang, China)
      • Key Laboratory for Anisotropy and Texture of Materials
      Feng-t’ien, Liaoning, China
  • 2010–2014
    • Shanghai Jiao Tong University
      • • Bio-X Institute
      • • Antai College of Economics and Management
      • • School of Agriculture and Biology
      Shanghai, Shanghai Shi, China
    • Huaibei Normal University
      Hua-pei-ts’un, Shanxi Sheng, China
    • Tohoku University
      • Institute for Materials Research
      Sendai-shi, Miyagi-ken, Japan
    • Jiangsu Academy of Agricultural Sciences
      Nan-ching-hsü, Jiangxi Sheng, China
  • 2008–2014
    • Qingdao University of Science and Technology
      Tsingtao, Shandong Sheng, China
    • Hawaii Agriculture Research Center
      Honolulu, Hawaii, United States
    • GNS Science
      Lower Hutt City, Wellington, New Zealand
    • Imperial College London
      Londinium, England, United Kingdom
    • University of Hawai'i System
      Honolulu, Hawaii, United States
    • University of Hawaiʻi at Hilo
      • Department of Natural Science
      Hilo, HI, United States
  • 2007–2014
    • Chinese Academy of Sciences
      • • Institute of Process Engineering
      • • Institute of Biophysics
      • • Key Laboratory of Computer System and Architecture
      Peping, Beijing, China
    • Queen's University Belfast
      • School of Pharmacy
      Béal Feirste, N Ireland, United Kingdom
    • University of Victoria
      • Department of Psychology
      Victoria, British Columbia, Canada
  • 2006–2014
    • Sun Yat-Sen University
      • Proteomics Lab
      Shengcheng, Guangdong, China
    • Peking University
      • Department of Psychology
      Peping, Beijing, China
  • 2005–2014
    • Nanjing Medical University
      • Key Laboratory of Reproductive Medicine
      Nan-ching, Jiangsu Sheng, China
    • Fourth Military Medical University
      • • Department of Dermatology
      • • Department of Biochemistry and Molecular Biology
      Xi’an, Liaoning, China
    • Robert Koch Institut
      • ZBS 3: Microbial Toxins
      Berlín, Berlin, Germany
    • Freie Universität Berlin
      Berlín, Berlin, Germany
  • 2004–2014
    • Tsinghua University
      • • Department of Basic Medical Sciences
      • • Department of Electronic Engineering
      • • Department of Chemical Engineering
      Peping, Beijing, China
    • Nankai University
      • • Department of Genetics and Cell Biology
      • • TEDA School of Biological Science and Biotechnology
      • • College of Life Sciences
      T’ien-ching-shih, Tianjin Shi, China
  • 1999–2014
    • Sun Yat-Sen University of Medical Sciences
      Shengcheng, Guangdong, China
    • University of Melbourne
      • Department of Microbiology and Immunology
      Melbourne, Victoria, Australia
  • 2010–2013
    • Shandong University
      • • Key Laboratory for Colloid and Interface Chemistry
      • • Department of Physics
      Chi-nan-shih, Shandong Sheng, China
  • 2009–2013
    • Liaocheng Teachers University
      Tungchangfu, Shandong Sheng, China
    • Tongji University
      • Department of Material Science and Engineering
      Shanghai, Shanghai Shi, China
    • National Institute on Alcohol Abuse and Alcoholism
      Maryland, United States
    • Indian Institute of Technology Bombay
      • Department of Biosciences & Bioengineering
      Mumbai, State of Maharashtra, India
    • Capital Medical University
      • Department of Otorhinolaryngology Head and Neck Surgery
      Peping, Beijing, China
  • 2007–2013
    • Technical Institute of Physics and Chemistry
      Peping, Beijing, China
  • 2006–2013
    • Northeast Institute of Geography and Agroecology
      • • Institute of Biophysics
      • • Institute of Computing Technology
      Beijing, Beijing Shi, China
  • 2012
    • General Hospital of Jinan Military Region
      Chi-nan-shih, Shandong Sheng, China
    • Instituto de Salud Carlos III
      Madrid, Madrid, Spain
    • Nanjing University of Information Science & Technology
      Nan-ching, Jiangsu Sheng, China
    • Beijing Institute Of Technology
      Peping, Beijing, China
    • Shanghai Municipal Center for Disease Control and Prevention
      Shanghai, Shanghai Shi, China
    • Carl von Ossietzky Universität Oldenburg
      Oldenburg, Lower Saxony, Germany
    • Fred Hutchinson Cancer Research Center
      • Division of Clinical Research
      Seattle, Washington, United States
    • Huazhong University of Science and Technology
      • Key Laboratory of Organ Transplantation , MOE
      Wu-han-shih, Hubei, China
  • 2011–2012
    • Jiangnan University
      • • School of Biotechnology
      • • School of Food Science and Technology
      Wu-hsi, Jiangsu Sheng, China
    • Chinese Center For Disease Control And Prevention
      Peping, Beijing, China
  • 2008–2012
    • Queen's University
      • • Division of Rheumatology
      • • Department of Medicine
      Kingston, Ontario, Canada
  • 2007–2012
    • Fudan University
      • Institutes of Biomedical Sciences
      Shanghai, Shanghai Shi, China
  • 2005–2012
    • Russian Academy of Sciences
      • Zelinsky Institute of Organic Chemistry
      Moscow, Moscow, Russia
  • 2002–2012
    • China Agricultural University
      • • Department of Microbiology and Immunology
      • • College of Resources and Environmental Sciences
      • • College of Biological Sciences
      Peping, Beijing, China
  • 2008–2011
    • Shanghai University of Traditional Chinese Medicine
      • Institute of Liver Diseases
      Shanghai, Shanghai Shi, China
  • 2005–2011
    • National Institutes of Health
      • • Laboratory of Liver Diseases
      • • Laboratory of Physiologic Studies
      Bethesda, MD, United States
  • 1998–2011
    • University of Sydney
      • School of Molecular Bioscience
      Sydney, New South Wales, Australia
  • 2008–2010
    • Salk Institute
      • Structural Biology Laboratory
      La Jolla, California, United States
  • 2006–2010
    • Tianjin University of Science and Technology
      • Faculty of Food Engineering and Biotechnology
      T’ien-ching-shih, Tianjin Shi, China
  • 2004–2007
    • Jilin University
      • State Key Laboratory of Inorganic Synthesis and Preparative
      Jilin, Jilin Sheng, China