L Zhang

Publications (1)2.18 Total impact

• Article: Effect of heat stress on the expression levels of receptor activator of NF-κB ligand and osteoprotegerin in human periodontal ligament cells.

  L Zhang, X Zhou, Q Wang, Y Wang, L Tang, D Huang
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  ABSTRACT:   To investigate the expression levels of the receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) in human periodontal ligament fibroblasts (PDLs) when stimulated with heat.   Periodontal ligament fibroblasts were subjected to various temperature increases for 5 min and then maintained at 37 °C. After that, cell viability was determined using an MTT assay. The expression levels of RANKL and OPG were investigated using real-time RT-PCR and ELISA. As a control, the cells were cultured at 37 °C. Data were analysed using one-way anova at a significant level of P = 0.05. Results  Cell viability was reduced significantly in the heated groups (P < 0.05). Heat stress downregulated the mRNA expression levels of RANKL and OPG (P < 0.05). When the cells were heated at 39 °C, the protein release of OPG was increased (P < 0.05). There were no significant differences between the heated groups and the control in the release of soluble RANKL (P > 0.05). The relative RANKL/OPG expression ratios were decreased at 39, 43 and 50 °C (P < 0.05), but increased at 47 °C (P < 0.05).   Heat influenced the balance between RANKL and OPG in PDLs. Low heat downregulated their relative ratio, whilst high heat upregulated it.
  International Endodontic Journal 09/2011; 45(1):68-75. · 2.18 Impact Factor