Li Zhang

Fourth Military Medical University, Xi’an, Liaoning, China

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Publications (288)1045.58 Total impact

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    ABSTRACT: An ultrasensitive protocol for surface plasma resonance (SPR) detection of adenosine is designed with the aptamer-based target-triggering cascade multiple cycle amplification, and streptavidin-coated Au-NPs (Au NPs-SA) enhancement to enhance the SPR signals. The cascade amplification process consists of the aptamer-based target-triggering nicking enzyme signaling amplification (T-NESA), the nicking enzyme signaling amplification (NESA) and the hybridization chain reaction (HCR), the whole circle amplification process is triggered by the target recognition of adenosine. Upon recognition of the aptamer to target adenosine, DNA s1 is released from the aptamer and then hybridizes with hairpin DNA (HP1). The DNA s1 can be dissociated from HP1 under the reaction of nicking endonuclease to initiate the next hybridization and cleavage process. Moreover, the products of the upstream cycle (T-NESA) (DNA s2 and s3) could act as the "DNA trigger" of the downstream cycle (NESA and HCR) to generate further signal amplification, resulting in the immobilization of abundant Au NPs-SA on the gold substrate, and thus significant SPR enhancement is achieved due to the electronic coupling interaction between the localized surface plasma of Au NPs and the surface plasma wave. This detection method exhibits excellent specificity and sensitivity towards adenosine with a detection limit of 4 fM. The high sensitivity and specificity make this method a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine.
    Analytical Chemistry 12/2014; · 5.83 Impact Factor
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    ABSTRACT: Autoimmune pancreatitis (AIP) is a chronic inflammatory disease of pancreas. We evaluated the clinical manifestations, imaging, and histological presentations of AIP in Chinese patients, and investigated the roles of immunoglobulin E (IgE) and allergic diseases in the diagnosis and pathogenesis of AIP. The clinical records of 22 patients diagnosed with AIP were reviewed and analyzed. All patients with AIP fulfilled the 2006 revised diagnostic criteria proposed by Japan Pancreas Society or the Korean Criteria for AIP. Half (11/22) of AIP patients had allergic diseases. Twenty-one patients had elevated serum IgE levels, and 14 patients had IgE levels more than 3 times that of normal. There were no significant differences between the patients with higher or lower IgE, with or without allergic disease, in clinical features, laboratory tests, diffuse or focal lesions, or the choice of treatment methods; however, more complaints of body weight loss were observed in patients with higher IgE levels. Patients with higher IgE levels and with allergic diseases were more likely to have onset in March, April, May, August, September, or October. IgE levels decreased after therapy, but increased again during recurrence. Increased number of mast cells was found in the pancreatic tissue in AIP. IgE maybe a useful marker for monitoring therapeutic response and recurrence of AIP. Allergic processes may play an important role in the pathogenesis of AIP.
    Chinese medical journal. 12/2014; 127(23):4104-9.
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    ABSTRACT: To assess the 24-month efficacy after booster vaccination with 3 doses of hepatitis B vaccine among low-response adults in Zhangqiu county of Shandong province. A total of 24 237 adults aged 18-49 years old, never received HepB vaccination, without HBV infection history, and had been living at 3 towns of Zhangqiu county in Shandong province for more than half a year in september, 2009, were collected blood samples of 3-5 ml. A total of 11 590 adults who were negative for hepatitis B virus (HBV) surface antigen (HBsAg) , antibody to HBsAg (Anti-HBs) and antibody to HBV core antigen (Anti-HBc), were divided into four groups randomly and were vaccinated following the schedule of 0-1-6 with 20 µg hepatitis B vaccine made by recombinant deoxyribonucleic acid techniques in Saccharomyces cerevisiae (HepB-SC), 20 µg hepatitis B vaccine made by Chinese hamster ovary cell (HepB-CHO), 10 µg HepB-SC and 10 µg hepatitis B vaccine made by recombinant deoxyribonucleic acid techniques in Hansenula Polymorpha (HepB-HP), respectively. The adults who were low-response to the primary hepatitis B vaccination (10 mU/ml ≤ anti-HBs<100 mU/ml) were divided into four groups by cluster random sampling. These groups were revaccinated with 3-dose of above-mentioned four kinds of HepB respectively. Blood samples were drawn from 1month (T1) and 24 month(T24) after the 3 dose revaccination, respectively. Anti-HBs and anti-HBc was detected by Chemiluminescence Microparticle Imunoassay (CMIA). Out of the 8 592 adults who have accepted the primary vaccination of hepatitis B and been collected the blood samples, 1 306 subjects showed low-response. A total of 718 low-response subjects were collected blood samples after T1 and T24 following 3 doses of booster vaccination. The proportion of the four groups was 32.3% (232/718), 25.8% (185/718) , 19.3% (139/718) , 22.6% (162/718) , respectively. The average proportion of anti-HBs ≥ 100 mIU/ml were decreased from 77.58% after T1 to 35.63% after T24 (χ(2) = 256.87, P < 0.01). The proportion of anti-HBs ≥ 100 mIU/ml in T24 were 38.8% (90/177), 39.5% (73/185), 25.2% (35/139) and 35.8% (58/162) in four groups, respectively. The proportion of anti-HBs>100 mIU/ml in T24 was significantly different among groups (χ(2) = 8.81, P = 0.032). The average geometric mean concentration (GMC) was significantly reduced from 443.53 mIU/ml after T1 to 48.98 mIU/ml after T24 (F = 439.41, P < 0.01). The GMC was 60.26 (45.71-77.62), 1.29 (38.90-69.18) , 35.48 (25.70-48.98) and 46.77 (33.88-6.07) mIU/ml in four groups, respectively (F = 1.97, P = 0.117) . Compared with vaccinated 20 µg HepB-SC, the proportion of anti-HBs ≥ 100 mIU/ml and GMC was 0.56(0.35-0.91) and -0.20 (-0.39--0.02) times. The positive of HBsAg was not found and the positive rate of anti-HBc was 2.6% (18/692)in T24. Protective antibody following booster vaccination with three doses of hepatitis B vaccines among low-response adults after 2 years fade faster. Antibody level of anti-HBs in T24 was corrected with the booster vaccine type and age. 20 µgHepB-SC seemed better than 10 µg HepB-SC.
    Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 12/2014; 48(12):1043-1047.
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    ABSTRACT: An exonuclease III-assisted recycling amplification (ERA) coupled with fluorescent DNA-scaffolded silver nanoclusters (Ag NCs) for sensitive determination of hepatitis B virus (HBV) DNA is proposed. The sensing mechanism is based on the employment of two different molecular beacons (MBs), MB1 and MB2. In the absence of targets, the MBs self-hybridize into stable stem-loop structures with exonuclease III (Exo III) cleaving resistance, and the MB2 containing a C-rich loop can be utilized to synthesize Ag NCs with high fluorescence signal. In the presence of targets, the targets can bind with MB1 to form partly duplex structure. With the cleaving of Exo III, the liberated target DNA can initiate the cycle of MB1-target hybridization, while the generated trigger DNA can hybridize with MB2, inducing a conformational change of MB2 from hairpin to single-stranded structure. The structural change of MB2 would influence the formation of Ag NCs, thus producing weak fluorescence signal, and the released trigger DNA could activate the cycle of MB2-trigger hybridization. The present method for HBV DNA analysis exhibits a detection limit of 0.97 nM (S/N = 3) with high specificity, and demonstrates its applicability for the HBV detection in human serum.
    Sensors and Actuators B Chemical 12/2014; 205:219–226. · 3.84 Impact Factor
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    ABSTRACT: Objective: The aim of the study was to estimate long-term cost effectiveness of a hepatitis B vaccination catch-up program among children born between 1994 and 2001 (when they were 8 15 years old) in Shandong province, China, to provide information for nationwide evaluation and future policy making. Methods: We determined the cost-effectiveness of the catch-up program compared with the status quo (no catch-up program). We combined a Decision Tree model and a Markov model to simulate vaccination and clinical progression after hepatitis B virus (HBV) infection. Parameters in the models were from the literature, a field survey, program files, and the National Notifiable Disease Reporting System (NNDRS). The incremental cost effectiveness ratio (ICER) was used to compare the two alternative strategies. One-way sensitivity analysis, two-way sensitivity analysis, and probability sensitivity analysis were used to assess parameter uncertainties. Results: The catch-up program was dominant compared with the status quo. Using a total of 5.53 million doses of vaccines, the catch-up program could prevent 21,865 cases of symptomatic acute hepatitis B, 3,088 carrier states with positive hepatitis B surface antigen (HBsAg), and 812 deaths due to HBV infection. The catch-up program could add 28,888 quality-adjusted life years (QALYs) and save $192.01 million in the targeted population in the future. The models were robust, considering parameter uncertainties. Conclusion: The catch-up program in Shandong province among children born between 1994 and 2002 2001 was 'very cost-saving.' It could save life years and reduce total future costs. Our study supported the desirability and impact of such a catch-up program throughout China.
    Human Vaccines and Therapeutics 11/2014; · 3.64 Impact Factor
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    ABSTRACT: A facile and quick route for the chemical reduction of graphene oxide (GO) using In powder as a reductant has been established. The reduction of GO by In powder is traced by UV-visible absorption spectroscopy, and the obtained reduced graphene oxide (rGO) is analyzed. The In3+ ions produced during the reaction between the GO and the In powder are chemically transformed to In2O3 and then form In2O3/rGO hybrids. The In2O3/rGO hybrids are used as electrode materials and their electrochemical performance are studied using cyclic voltammetry and galvanostatic charge/discharge. The In2O3/rGO hybrids demonstrate excellent electrochemical performance and their highest specific capacitance is 178.8 F g-1 which is much higher than that of either In2O3 or rGO. In addition, the In2O3/rGO hybrids are also very stable.
    Journal of Power Sources 10/2014; 266. · 5.21 Impact Factor
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    ABSTRACT: Curcumin, traditionally used as food and medicinal purposes, has recently been reported to have protective efficacy against hypoxia. Hypoxia is one of the important reactive factors in tumor metastasis, which is a key problem in clinical thyroid cancer therapy. In present study, we investigate the anti-metastatic effect of curcumin on the K1 papillary thyroid cancer cells as well as its potential mechanisms. The results show that curcumin effectively inhibits hypoxia-induced reactive oxygen species (ROS) upregulation and significantly decreases the mRNA and protein expression levels of hypoxia-inducible factor-1α (HIF-1α) in K1 cells. Curcumin also decreases the DNA binding ability of HIF-1α to hypoxia response element (HRE). Furthermore, curcumin enhances E-cadherin expression, inhibits metalloproteinase-9 (MMP-9) enzyme activity, and weakens K1 cells migration under hypoxic conditions. In summary, these results indicate that curcumin possesses a potent anti-metastatic effect and might be an effective tumoristatic agent for the treatment of aggressive papillary thyroid cancers.
    Experimental Biology and Medicine 10/2014; · 2.23 Impact Factor
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    ABSTRACT: Prostaglandin E2 (PGE2) has been implicated in cell invasion in hepatocellular carcinoma (HCC), via increased β1-integrin expression and cell migration; however, the mechanism remains unclear. PGE2 exerts its effects via four subtypes of the E prostanoid receptor (EP receptor 1-4). The present study investigated the effect of EP1 receptor activation on β1-integrin expression and cell migration in HCC. Cell migration increased by 60% in cells treated with 17-PT-PGE2 (EP1 agonist), which was suppressed by pretreatment with a β1-integrin polyclonal antibody. PGE2 increased β1-integrin expression by approximately 2-fold. EP1 receptor transfection or treatment with 17-PT-PGE2 mimicked the effect of PGE2 treatment. EP1 siRNA blocked PGE2-mediated β1-integrin expression. 17-PT-PGE2 treatment induced PKC and NF-κB activation; PKC and NF-κB inhibitors suppressed 17-PT-PGE2-mediated β1-integrin expression. FoxC2, a β1-integrin transcription factor, was also upregulated by 17-PT-PGE2. NF-κB inhibitor suppressed 17-PT-PGE2-mediated FoxC2 upregulation. Immunohistochemistry showed p65, FoxC2, EP1 receptor and β1-integrin were all highly expressed in the HCC cases. This study suggested that PGE2 upregulates β1-integrin expression and cell migration in HCC cells by activating the PKC/NF-κB signaling pathway. Targeting PGE2/EP1/PKC/NF-κB/FoxC2/β1-integrin pathway may represent a new therapeutic strategy for the prevention and treatment of this cancer.
    Scientific Reports 10/2014; 4:6538. · 5.08 Impact Factor
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    ABSTRACT: The relationship between hydrophobicity and the protective effect of whey protein hydrolysates (WPHs) against oxidative stress was studied. Whey protein was first hydrolysed by pepsin and trypsin to obtain WPHs. After absorbed by macroporous adsorption resin DA201-C, three fractions named as M20, M40, and M60 were eluted by various concentrations of ethanol. The hydrophobicity showed a trend of increase from M20 to M60. Antioxidant ability test in vitro indicated that all the three components of WPHs displayed reasonably good antioxidant ability. Moreover, with the increase of hydrophobicity, antioxidant ability of WPHs improved significantly. Then rat pheochromocytoma line 12 (PC12) cells oxidative model was built to evaluate the suppression of oxidative stress of three components on PC12 cells induced by H2O2. Morphological alterations, cell viability, apoptosis rate, and intracellular antioxidase system tests all indicated that WPHs exert significant protection on PC cells against H2O2-induced damage. Among them, M60 had the highest protective effect by increasing 19·3% cell survival and reducing 28·6% cell apoptosis. These results suggested hydrophobicity of WPHs was contributing to the antioxidant ability and the protective effect against oxidative damage.
    Journal of Dairy Research 10/2014; · 1.39 Impact Factor
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    ABSTRACT: Bimetallic palladium–silver nanoparticles (NPs) supported on reduced oxide graphene (RGO) with different Pd/Ag ratios (Pd–Ag/RGO) were prepared by an easy green method which did not use any additional reducing agents or a dispersing agent. During the process, simultaneous redox reactions between AgNO3, K2PdCl4 and graphene oxide (GO) led to bimetallic Pd–Ag NPs. The morphology and composition of the Pd–Ag/RGO were characterized by transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermogravimetric analysis and Raman spectroscopy. Cyclic voltammetry and chronoamperometry were used to investigate the electrochemical activities and stabilities of these Pd–Ag/RGO catalysts for the electro-oxidation of methanol and ethanol in alkaline media. Among the different Pd/Ag ratios, the Pd–Ag (1:1)/RGO had the best catalytic activities and stability. So it is a promising catalyst for direct alcohol fuel cell applications.
    Journal of Power Sources 10/2014; 263:13–21. · 5.21 Impact Factor
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    ABSTRACT: To compare the antibody response between adults with hepatitis B virus (HBV) core antibody (anti-HBc) single positivity and healthy adults after primary immunization and revaccination of hepatitis B vaccine(HepB). Adults aged from 18 to 49 who were both negative for HBV surface antigen (HBsAg) and antibody to HBsAg (anti-HBs), but positive for anti-HBc and narrated no history of HepB immunization by themselves, were selected as single anti-HBc positive group ('anti-HBc alone'). Adults who were negative for HBsAg, anti-HBs and anti-HBc, with age differences within 2 years, and same gender under the 1 : 1 matching program, were selected to form the control group. Both groups were vaccinated on 0-1-6 schedule with the same HepB. Those who were non-response to HepB at primary immunization were revaccination on 0-1-6 schedule. Response rates and geometric mean concentrations (GMC) between the two groups were compared. In total, the number of anticipants were 228 pairs. Rates on non-response, low-response, normal-response and high-response after the primary immunization were 8.77% , 11.84%, 31.14% and 48.25% in the control group respectively. The corresponding rates were 8.33%, 30.70%, 35.96% and 25.00% in the 'anti-HBc alone'. The rate of low-response in the control group was lower than that in the 'anti-HBc alone' (χ(2) = 22.28, P < 0.01), while the rate of high-response was higher than that in the control group (χ(2) = 24.43, P < 0.01). GMC of anti-HBs in the control group (534.07 mIU/ml) was higher than that in the 'anti-HBc alone' (183.99 mIU/ml) (u = 4.42, P < 0.01). The anti-HBs conversion rates were 82.35% and 41.18% in the control group and in the 'anti-HBc alone' respectively after the first-dose revaccination, but increased to 90.00% and 82.35% after the third-dose revaccination. The anti-HBs conversion rates in the control group were higher than that in the 'anti-HBc alone' after the first-dose revaccination (P < 0.05), while there was no difference seen between the two groups after the third-dose revaccination (P > 0.05). Immune response in the anti-HBc positive adults after primary immunization was weaker than that in common adults. However, immune response induced by HepB was enough to prevent them from infecting HBV. The rates of response showed an obvious increase after revaccination, hence the same HepB immunization strategy could be used.
    10/2014; 35(10):1091-4.
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    ABSTRACT: The fuzzy c-means (FCM) algorithm is a widely applied clustering technique, but the implicit assumption that each attribute of the object data has equal importance affects the clustering performance. At present, attribute weighted fuzzy clustering has became a very active area of research, and numerous approaches that develop numerical weights have been combined into fuzzy clustering. In this paper, interval number is introduced for attribute weighting in the weighted fuzzy c-means (WFCM) clustering, and it is illustrated that interval weighting can obtain appropriate weights more easily from the viewpoint of geometric probability. Moreover, a genetic heuristic strategy for attribute weight searching is proposed to guide the alternating optimization (AO) of WFCM, and improved attribute weights in interval-constrained ranges and reasonable data partition can be obtained simultaneously. The experimental results demonstrate that the proposed algorithm is superior in clustering performance. It reveals that the interval weighted clustering can act as an optimization operator on the basis of the traditional numerical weighted clustering, and the effects of interval weight perturbation on clustering performance can be decreased.
    Expert Systems with Applications 10/2014; 41(13):5960–5971. · 1.97 Impact Factor
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    ABSTRACT: A label-free, sensitive and simple method to detect protein kinase based on the selective aggregation of phosphorylated peptide-gold nanoclusters (peptide-AuNCs) triggered by Zr(4+) ion coordination is developed. The AuNCs were synthesized by peptide without any strong reducing agents, which prevent peptides from being disrupted. Under optimal conditions, a linear relationship between the decreased PL intensity of peptide-AuNCs and the concentration of casein kinase II (CK2) in the range of 0.08-2.0unitmL(-1) with a detection limit of 0.027unitmL(-1) (3σ) was obtained. The feasibility of this AuNCs-based sensor was further demonstrated by the assessment of kinase inhibition by ellagic acid, 5,6-dichlorobenzimidazole-1-β-d-ribofuranoside, emodin, and quercetin in human serum. As expected, the PL intensity increased with increasing inhibitor efficiency in the presence of inhibitors. The IC50 value (inhibitor concentration producing 50% inhibition) for ellagic acid was estimated to be 0.045μM. With more sophisticated design of the peptide substrate sequences, the detection of other enzymes will be realized. With characteristics of homogeneous, facile, universal, label-free, and applicable for kinase assay, the proposed sensor provides potential application in kinase-related biochemical fundamental research and inhibitor screening.
    Biosensors & Bioelectronics 09/2014; 64C:234-240. · 6.45 Impact Factor
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    ABSTRACT: Podocyte damage and loss together have an important role in the pathogenesis and progression of glomerulonephritis. Glomerulonephritis patients and healthy controls were enrolled in this study. Biochemical, clinical and experimental procedures included measurement of total urinary protein, renal biopsy and gene expression analysis of the receptor activator of NF-kappaB (RANK). The urinary mRNA levels of RANK were significantly higher in the glomerulonephritis group compared to the controls. The urinary RANK level of glomerular subtypes was correlated significantly with proteinuria. The calculated area of RANK mRNA levels under the curve was 0.61 for minimal change disease (MCD), 0.97 for membranous nephropathy (MN), 0.65 for IgA nephropathy (IgAN), 0.70 for lupus nephritis (LN) and 0.70 for focal segmental glomerulosclerosis (FSGS). The urinary mRNA of RANK might be used to differentiate histologic subtypes of glomerulonephritis, particularly between MCD and MN.
    Biomarkers 08/2014; · 2.52 Impact Factor
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    ABSTRACT: A series of lanthanide thiogermanates [Ln(dien)3]2[Ge2S6]Cl2 [Ln = Pr (Ia), Sm (Ib), Gd (Ic), Dy (Id); dien = diethylenetriamine], [Er2(dien)4(μ-OH)2][Ge2S6] (II) and [Ho(trien)(en)GeS3(SH)] (III, trien = triethylenetetramine, en = ethylenediamine) have been hydrothermally synthesized and structurally characterized. The structures of Ia–d consist of isolated [Ln(dien)3]3+ cations, [Ge2S6]4− anions built up from the connection of two [GeS4] tetrahedra sharing a common edge and Cl− ions. II contains binuclear [Er2(dien)4(μ-OH)2]4+ cations constructed by the linkage of [Er(dien)2]3+ ions and –OH bridging groups, and [Ge2S6]4− anions. III contains neutral holmium-centred complexes, where the unusual protonated tetrahedral anion [GeS3(SH)]3− acts as a chelating ligand to complex the [Ho(en)(trien)]3+ cation. A systematic investigation of six lanthanide thiogermanates and four reported compounds revealed that both the well-known lanthanide contraction and different chelating organic amines have a significant influence on the formation of lanthanide thiogermanates under solvothermal conditions. Density functional theory calculation for III has also been performed and the absorption edges of all compounds have been investigated by UV-vis spectroscopy.
    RSC Advances 08/2014; 4(73). · 3.71 Impact Factor
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    ABSTRACT: Post-exposure prophylaxis with hepatitis B vaccine (HepB) alone is highly effective in preventing perinatal hepatitis B virus (HBV) transmission and the World Health Organization recommends administering HepB to all infants within 24 h after delivery. Maternal screening for HBsAg and administration of hepatitis B immune globulin (HBIG) in addition to HepB for infants born to HBsAg-positive pregnant women can increase the effectiveness of post-exposure prophylaxis for perinatal HBV transmission. In Shangdong Province, China which has a high prevalence of chronic HBV infection, HepB birth dose and HBIG were integrated into the routine childhood immunization program in 2002 and July 2011 respectively. We assessed progress toward implementation of these measures. Hospital-based reporting demonstrated an increase in maternal screening from 70.7% to 96.9% from 2004-2012; HepB birth dose coverage (within 24 h) remained high (96.3-97.1%) during this period. For infants with known HBsAg-positive mothers, the coverage of HBIG increased from 85.0% (before July 2011) to 92.1% (after July 2011). However, HBIG coverage in western areas of Shandong Province remained at 81.1% among infants with known HBsAg-positive mothers. Preterm/low-birth-weight and illness after birth were the most commonly reported reasons for delay in the first dose of HepB to >24 h of birth. Additional education on the safety and immune protection from HepB and HBIG might help to correct delays in administering the HepB birth dose and low HBIG coverage in the western areas of the Shandong Province.
    Human Vaccines and Therapeutics 08/2014; · 3.64 Impact Factor
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    ABSTRACT: Hepatocellular carcinoma (HCC) represents a major health problem worldwide. Prostaglandin E2 (PGE2), the predominant product of cyclooxygenase-2, has been implicated in hepatocarcinogenesis. However, the underlying molecular mechanisms remain to be further elucidated. c-myc, a cellular proto-oncogene, is activated or overexpressed in many types of human cancer, including HCC. The present study was designed to investigate the internal relationship and molecular mechanisms between PGE2 and c-Myc in HCC, and to define its role in HCC cell growth and invasion. Our results showed that PGE2 significantly upregulated c-Myc expression at both the mRNA and protein levels, and knockdown of c-Myc blocked PGE2-induced HCC cell growth and invasive ability in human HCC Huh-7 cells. The effect of PGE2 on c-Myc expression was mainly through the EP4 receptor, and EP4 receptor-mediated c-Myc protein upregulation largely depended on de novo biosynthesis of c-Myc mRNA and its protein. EP4 receptor signaling activated GS/AC and increased the intracellular cAMP level in Huh-7 cells. The adenylate cyclase (AC) activator forskolin mimicked the effects of the EP4 receptor agonist on c-Myc expression, while the AC inhibitor SQ22536 reduced EP4 receptor-mediated c-Myc upregulation. These data confirm the involvement of the GS/AC/cAMP pathway in EP4 receptor-mediated c-Myc upregulation. Moreover, the phosphorylation levels of CREB protein were markedly elevated by EP4 receptor signaling, and by using specific inhibitor and siRNA interference, we demonstrated that PKA/CREB was also involved in the EP4 receptor-mediated c-Myc upregulation. In summary, the present study revealed that PGE2 significantly upregulates c-Myc expression at both mRNA and protein levels through the EP4R/GS/AC/cAMP/PKA/CREB signaling pathway, thus promoting cell growth and invasion in HCC cells. Targeting of the PGE2/EP4R/c-Myc pathway may be a new therapeutic strategy to prevent and cure human HCC.
    Oncology reports. 08/2014;
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    ABSTRACT: Topographical modification at micro- and nanoscale is widely applied to enhance the tissue integration properties of biomaterials, but the underlying molecular mechanism is poorly understood. The biomaterial topography modulates cell functions via mechanotransduction of direct and indirect. We propose that N-cadherin may play a role in the topographically induced indirect mechanotransduction by regulating the β-catenin signaling. For confirmation, the cell functions, N-cadherin expression and β-catenin signaling activation of osteoblasts on titanium (Ti) surfaces with micro- or/and nanotopography are systemically compared with naive and N-cadherin down-regulating MC3T3-E1 cells. We find that the N-cadherin expression is reversely related to the intracellular β-catenin signaling and the N-cadherin/β-catenin signaling is modulated differentially by the micro- and nanotopography. The nanotopography significantly up-regulates the N-cadherin expression leading to lower β-catenin signaling activity and consequently depressed differentiation, whereas the microtopography down-regulates the N-cadherin expression resulting in enhanced β-catenin signaling and thus osteoblast differentiation. Artificial down-regulation of the N-cadherin expression can significantly up-regulate the β-catenin signaling and consequently enhance the osteoblast differentiation on all the Ti surfaces. The study for the first time clarifies the involvement of the N-cadherin/β-catenin interaction in the micro/nanotopography induced indirect mechanotransduction and provides a potentially new approach for biomaterial modification and biofunctionalization by down-regulating the cell N-cadherin expression to achieve improved clinical performance.
    Biomaterials 08/2014; 35(24):6206–6218. · 8.31 Impact Factor
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    ABSTRACT: A horseradish peroxidase (HRP)-mimicking DNAzyme sequence is first blocked by the triplex-based molecular beacon (tMB). Upon hybridization with single-stranded DNA inputs, triplex–helix molecular switch occurs, and the released product strand self-assembles into the hemin/G-quadruplex-HRP-mimicking DNAzyme that biocatalyzes the formation of a colored product and provides an output signal for the different logic gates. On the basis of this principle, a series of logic gates (OR, XOR, INHIBIT, and AND) have been developed. Moreover, a multilevel circuit (MC) that enforces an overall OR Boolean behavior is developed by connecting the AND and XOR logic gates. The logic output signals can be recognized by naked eyes, thus providing a flexible, secure, economic, and simple method for designing a complex DNA-based logic device.
    The Journal of Physical Chemistry C 06/2014; 118(26):14410–14417. · 4.84 Impact Factor
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    ABSTRACT: A simple method to fabricate Bi nanoparticles by using redox reactions between sodium borohydride and ammonium bismuth citrate in the presence of soluble starch in water phase was developed. The results show that soluble starch is better than PVP in stabilizing Bi nanoparticles. The as-prepared Bi nanoparticles were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray, and powder X-ray diffraction. The catalytic performance of the Bi nanoparticles for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of sodium borohydride was studied. The effects of sodium borohydride concentration, initial 4-NP concentration, catalyst dose, and reduction temperature were also investigated.
    Industrial & Engineering Chemistry Research 06/2014; 53(26):10576–10582. · 2.24 Impact Factor

Publication Stats

5k Citations
1,045.58 Total Impact Points


  • 2014
    • Fourth Military Medical University
      • School of Stomatology
      Xi’an, Liaoning, China
    • Chongqing Normal University
      Ch’ung-ch’ing-shih, Chongqing Shi, China
    • Guangdong Center for Disease Control and Prevention
      Shengcheng, Guangdong, China
  • 2013–2014
    • Tianjin University
      • • Department of Chemistry
      • • School of Science
      T’ien-ching-shih, Tianjin Shi, China
    • Guangdong Academy of Medical Sciences and General Hospital
      Shengcheng, Guangdong, China
    • Shanghai Jiao Tong University
      • Center of Instrumental Analysis
      Shanghai, Shanghai Shi, China
    • Southern Medical University
      Shengcheng, Guangdong, China
    • Shandong University
      • Center for Health Management & Policy
      Chi-nan-shih, Shandong Sheng, China
    • Peking Union Medical College Hospital
      Peping, Beijing, China
  • 2011–2014
    • Nanchang University
      Nan-ch’ang-shih, Jiangxi Sheng, China
    • Liaoning University
      Feng-t’ien, Liaoning, China
    • Loyola University Maryland
      Baltimore, Maryland, United States
    • Peking University Third Hospital
      Peping, Beijing, China
    • Logistical College of Chinese People's Armed Police Force
      T’ien-ching-shih, Tianjin Shi, China
  • 2007–2014
    • Jiangsu Institute of Nuclear Medicine
      Jiangqun, Qinghai Sheng, China
    • Chinese Academy of Sciences
      • State Key Laboratory of Drug Research
      Peping, Beijing, China
    • Tsinghua University
      • Department of Environmental Engineering
      Peping, Beijing, China
  • 2006–2014
    • Nanjing Medical University
      • • Department of Pathology
      • • Key Laboratory of Reproductive Medicine
      Nan-ching, Jiangsu Sheng, China
  • 2002–2014
    • Henry Ford Hospital
      • Department of Neurology
      Detroit, Michigan, United States
    • Polish Academy of Sciences
      Warszawa, Masovian Voivodeship, Poland
  • 2012–2013
    • Sun Yat-Sen University
      Shengcheng, Guangdong, China
  • 2001–2013
    • Henry Ford Health System
      • Department of Neurology
      Detroit, Michigan, United States
  • 2010–2012
    • Chinese Center For Disease Control And Prevention
      Peping, Beijing, China
    • Oakland University
      • Department of Physics
      Rochester, MI, United States
  • 2006–2012
    • Northeast Institute of Geography and Agroecology
      • State Key Laboratory of Drug Research
      Beijing, Beijing Shi, China
  • 2006–2011
    • University of Maryland, Baltimore
      • • Center for Vascular and Inflammatory Diseases
      • • Department of Physiology
      • • Department of Medicine
      Baltimore, MD, United States
  • 2009
    • Jiangsu Provincial Center for Disease Control and Prevention
      Chiang-tu, Jiangsu Sheng, China
  • 2007–2009
    • University of Science and Technology, Beijing
      Peping, Beijing, China
  • 2006–2009
    • Dalian University of Technology
      • School of Electronic and Information Engineering
      Lü-ta-shih, Liaoning, China
    • Shanghai Institutes for Biological Sciences
      Shanghai, Shanghai Shi, China
  • 2008
    • University of Notre Dame
      • Department of Chemistry and Biochemistry
      South Bend, Indiana, United States
    • University of Kentucky
      • Department of Biology
      Lexington, KY, United States
  • 2005–2006
    • Nanjing University
      • State Key Laboratory of Pharmaceutical Biotechnology
      Nan-ching, Jiangsu Sheng, China
  • 2001–2006
    • University of Tsukuba
      • Institute of Applied Biochemistry
      Tsukuba, Ibaraki, Japan
  • 2003
    • The University of Tokyo
      • Faculty & Graduate School of Medicine
      Tokyo, Tokyo-to, Japan