Li Zhang

Beijing University of Chinese Medicine and Pharmacology, Peping, Beijing, China

Are you Li Zhang?

Claim your profile

Publications (434)676.69 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: An ideal substrate for surface-enhanced Raman scattering (SERS) detection should induce a high signal enhancement and be easy to synthesize. Here, we showed that gold nanoparticles (Au NPs) were self-assembled onto the DNA strands through electrostatic interactions and formed a well-defined DNA-Au hybrid structure with an interparticle gap of ca. 2-3 nm between two adjacent Au NPs, which could be used as active SERS substrates. Four different types of molecules, i.e. Rhodamine 6G, 4-aminothiophenol, pyridine and 2, 4, 6-trinitrotoluene were studied on these substrates. All the detection limits for each analyte on the DNA-Au hybrid substrate were at least one order of magnitude higher than that on the Au NPs solely without the self-assembly on DNA. This phenomenon of assembly-induced signal enhancement had been experimentally and theoretically demonstrated in this study.
    RSC Advances 09/2014; · 3.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Background Salvia-Nelumbinis naturalis (SNN), initially called Jiangzhi Granula as a formulae of Chinese medicinal decoction, has been used clinically to treat non-alcoholic fatty liver disease (NAFLD) and related syndromes. The mechanism of SNN action is unknown.Methods HepG2 cells were cultured in lipid-rich media supplemented with chemical components of SNN. Male Wistar rats (6 weeks of age) were fed a high calorie diet (15% fat, 15% sucrose, and 2% cholesterol) for eight weeks, and then treated with SNN for four weeks. Body and liver weight, lipids profiles, insulin and glucose levels, glucose and insulin tolerance were evaluated, the mRNA and protein expression of insulin receptor (InsR), insulin receptor substrate (IRS) 1/2, protein kinase B (PKB/Akt), protein expression of suppressor of cytokine signaling 3 (SOCS3), protein kinase C epsilon (PKC ¿) in liver tissue were analysed.ResultsTreatment with SNN components in lipid-laden HepG2 cells decreased lipid accumulation. Rats fed with a HC diet developed hepatosteatosis and accompanied hyperglycemia, hyperinsulinemia, hyperleptinemia, and diabetic dyslipidemia. Prolonged HC diet feeding resulted in parabolic response in plasma triglyceride (TG) concentrations, indicative of compromised hepatic production of TG-rich lipoproteins. HC diet feeding also resulted in impaired insulin sensitivity and hepatic insulin signalling. Administration of SNN extracts alleviated hepatosteatosis and conferred to a normolipoproteinemia profile in the HC diet-fed rats. The efficacy of SNN extract in improving liver function and insulin sensitivity was comparable to that of simvastatin or pioglitazone. The improved insulin signaling by SNN treatment was associated with increased IRS and Akt phosphorylation and decreased SOCS3 expression. However, SNN failed to inhibit the PKC ¿ expression in the liver.ConclusionsSNN is effective in reducing lipid accumulation in HepG2 cells and attenuating hepatosteatosis in HC diet-fed rats. Reduced hepatic lipid content in the rat liver was associated with improved insulin signalling.
    Journal of translational medicine. 08/2014; 12(1):236.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Long afterglow Sr4Al14O25:Eu,Dy phosphors were introduced into the TiO2 photoanode of CdS quantum dot-sensitized solar cells (QDSSCs) as both a scattering and down converting layer, and the photovoltaic performances of the cells were investigated. The results show that the cell with Sr4Al14O25:Eu,Dy achieves a power conversion efficiency of 1.40%, which is an increase of 38% compared to the cell without Sr4Al14O25:Eu,Dy (1.02%). The performance improvement is attributed to enhanced light harvesting via improved light absorption and scattering processes. After a single sun illumination for 1 min and subsequent removal of the light source, the cell with Sr4Al14O25:Eu,Dy could be driven even in the dark by the long persistent light from Sr4Al14O25:Eu,Dy.
    Dalton Transactions 08/2014; · 3.81 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The Chinese medicinal formula, Qinggan (QG) Huoxue (HX) Recipe (R) exerts a range of pharmacological effects, including reversible steatosis, decreased levels of inflammatory cytokines and lipid peroxidation resistance. The aim of the present study was to determine the specific mechanisms of QGHXR hepatoprotection through the lipopolysaccharide-Kupffer cell (LPS-KC) signal conduction pathway in rats with alcoholic liver disease (ALD). ALD rats were exposed to the compound factors, QGR and HXR. Hematoxylin and eosin staining was conducted to evaluate the pathological changes in the liver following QGHXR treatment and an enzyme-linked immunosorbent assay was performed to measure the content of tumor necrosis factor (TNF)-α in the plasma. Immunohistochemical staining was conducted to examine the expression of cell differentiation antigen (CD) 68 and 14. In addition, western blot analysis and reverse transcription-polymerase chain reaction were used to measure the expression of Toll-like receptor 4 (TLR4), phosphorylated-extracellular regulated protein kinases (p-ERK), nuclear factor (NF)-κB, CD14 and TNF-α. Following stimulation with the compound factors, the rats exhibited increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as marked pathological changes. Furthermore, the related molecules in the LPS-KC pathway were upregulated and QGHXR was identified to be effective in the LPS-KC signal conduction pathway in the ALD rats. QGHXR was superior to QGR and HXR in reducing the serum ALT and AST levels, regulating CD14, TLR4, NF-κB, ERK and TNF-α as well as improving the pathological changes. The results indicated that QGHXR therapy may provide a novel strategy for treating ALD via regulation of the related molecules in the LPS-KC signaling pathway.
    Experimental and therapeutic medicine 08/2014; 8(2):363-370. · 0.34 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Mesenchymal stem cells (MSCs) have the potential to differentiate into several cell types and provide an attractive source of autologous cells for regenerative medicine. However, their cellular biology is not fully understood. Similar to Ca(2+), extracellular Mg(2+) plays an important role in the functions of the skeletal system. Here, we examined the effects of extracellular Mg(2+) on the deposition of calcium phosphate matrix and Ca(2+) signaling with or without ATP stimulation in human bone marrow-derived mesenchymal stem cells (hBMSCs). We found that high extracellular Mg(2+) concentration ([Mg(2+)]e) inhibited extracellular matrix mineralization in hBMSCs in vitro. hBMSCs also produced a dose-dependent decrease in the frequency of calcium oscillations during [Mg(2+)]e elevation with a slight suppression on oscillation amplitude. In addition, spontaneous ATP release was inhibited under high [Mg(2+)]e levels and exogenous ATP addition stimulated oscillation reappear. Taken together, our results indicate that high [Mg(2+)]e modulates calcium oscillations via suppression of spontaneous ATP release and inactivates purinergic receptors, resulting in decreased extracellular mineralized matrix deposition in hBMSCs. Therefore, the high magnesium environment created by the rapid corrosion of Mg alloys may result in the dysfunction of calcium-dependent physiology processes and be disadvantageous to hBMSCs physiology.
    Biochemical and Biophysical Research Communications 07/2014; · 2.41 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Xenobiotic metabolome identification of Chinese herbal formula in biological systems is a very challenging task. Qingkailing injection is a typical Chinese herbal injection which is wildly used in clinic in China. However, the holistic metabolic fate of the ingredient from Qingkailing injection remains unclear. In this work, a metabolomic strategy for comprehensively elucidating Qingkailing injection derived prototype components and metabolites in rat urine conducted by hybrid linear ion trap-high resolution mass spectrometry was developed. High-performance liquid chromatography coupled with hybrid linear ion trap-high resolution mass spectrometry was developed to obtain the urine profiling between the control group and the Qingkailing injection treated group. Orthogonal partial least squares discriminate analysis was applied to distinguish the exogenous and the endogenous. In the S-plot, 37 xenobiotics derived from Qingkailing injection were found in urine, including 18 prototype compounds and 19 metabolites. The characterization of the prototype components and metabolites in rat's urine provided essential data for further pharmacological studies of Qingkailing injection. Our results indicated that the metabolomic approach was an effective tool to discover, screen and analyze the multiple prototype components and their metabolites from complicated traditional Chinese preparations in vivo.This article is protected by copyright. All rights reserved
    Journal of Separation Science 07/2014; · 2.59 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To determine the extent to which Chinese medical (CM) journals incorporate Consolidated Standards for Reporting of Trials (CONSORT) into their "instruction to authors".
    Chinese journal of integrative medicine. 07/2014; 20(7):510-5.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: AIM: Osteoblasts are key functional cells in the process of bone metabolic balance. Phytoestrogens have an important influence on the proliferation and differentiation of osteoblasts. Puerarin, a plant estrogen, has a wide range concentration in vitro on the function of osteoblasts. The current study investigates the effect of the phytoestrogen puerarin on the proliferation, differentiation, and mineralization of osteoblasts in vitro. METHODS: The calvaria bone of eight-ten Wistar rats which were born within 24 h were obtained in aseptic condition. After enzyme digestion, isolation, purified osteoblasts of rats were cultured for further study. The cells of the first to third generation were divided into a control group and a puerarin-treated group with 103–1010 mol•L1 puerarin. The cells were exposed to the medium containing a low level of carbohydrates, 10% (v/v) FBS for 24 h. After 1 to 4 days of culture, the OD values on the proliferation of osteoblasts in each group were determined by microplate reader. The cells were cultured in the medium containing 50 μg•mL1 vitamin C, 102 mol•L1 sodium glycerophosphate, 10% FBS and the medium was changed every 3 to 4 days. After 2 to 8 days of culture, expression of alkaline phosphatase were tested and compared by microplate reader. The mineral nodes of osteoblasts were dyed using alizarin red or improved Von Kossa way after four weeks. RESULTS: Compared with those in the 105–109 mol•L1 puerarin, the proliferation of osteoblasts, the expression of alkaline phosphatase, and the number of mineral nodes of osteoblasts were significantly decreased in the control group. The increase was the fastest in the third day, while on the fourth day it was decreased, and arrived at statistical significance compared with the alkaline phosphatase activities and control group. The 106 mol•L1 group was the most distinct, and formed the most mineralized nodule. Compared with the 103 mol•L1 puerarin group, those changes were markedly increased in the control group. CONCLUSIONS: Puerarin has proliferation, differentiation, and mineralization effects on osteoblasts in a dose-dependent manner, and has a double-way effect on the osteoblasts in vitro. A low-dose showed positive effects on the development of osteoblasts, and high-dose puerarin could inhibit the formation of bone.
    Chinese Journal of Natural Medicines 07/2014; 12(6):436-442.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Nanomaterials as enzyme mimics have received considerable attention as they can overcome some serious disadvantages associated with the natural enzymes. In recently developed Co3O4 nanoparticles as peroxidase mimics, the influence of the crystal plane on the catalytic performance has not been demonstrated. In order to better understand their crystal plane-dependent catalysis, the present study was initiated using three different Co3O4 nanomaterials, nanoplates, nanorods and nanocubes, as model systems. According to HRTEM, the predominantly exposed planes of nanoplates, nanorods and nanocubes are {112}, {110} and {100} planes, respectively. The catalytic activities were explored by using H2O2 and different organic substrates as the substrates of peroxidase mimics, and were investigated in-depth by steady-state kinetics and electrochemistry methods in depth. The results show that the peroxidase-like activity increases from nanocubes to nanoplates, via nanorods. The effect of external conditions such as pH and temperature on the three nanomaterials is the same, which indicates that the difference in their catalytic activities originates from their different shapes. The peroxidase-like catalytic activities of Co3O4 nanomaterials are crystal plane-dependent and follow the order: {112} ≫ {110} > {100}. The three crystal planes have different arrangements of surface atoms, thus exhibiting different abilities of electron transfer, which induce their different peroxidase-like catalytic activities. This investigation clarifies that the peroxidase-like activity of Co3O4 nanomaterials can be enhanced by shape control. These findings show that Co3O4 nanomaterials can serve as catalyst models for designing other catalysts.
    Physical Chemistry Chemical Physics 06/2014; · 3.83 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Silver nanowires with uniform diameters have been synthesized via the rapid sulfide mediated polyol method. The morphology, structure, and properties of as-prepared samples are characterized by UV-Visible spectroscopy, field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD), respectively. The mechanism of as-prepared Ag nanowires is provided and discussed. Moreover, as-prepared Ag nanowires are used as a Surface-Enhanced Raman Scattering (SERS) substrate to detect thiram pesticide. The results show that this substrate based on Ag nanowires exhibits high sensitivity and reproducibility for the thiram detection.
    Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy 06/2014; 133C:411-416. · 1.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Aim:Lapatinib is a dual inhibitor of EGFR and human epidermal growth factor receptor 2 (HER2), and used to treat advanced breast cancer. To overcome its poor water solubility, we constructed lapatinib-incorporated lipoprotein-like nanoparticles (LTNPs), and evaluated the particle characteristics and possible anti-breast cancer mechanisms.Methods:LTNPs (lapatinib bound to albumin as a core, and egg yolk lecithin forming a lipid corona) were prepared. The particle characteristics were investigated using transmission electron microscopy (TEM) and atomic force microscopy (AFM). The uptake and subcellular localization of LTNPs, as well as the effects of LTNPs on cell cycle were examined in BT-474 human breast cancer cells in vitro. Mice bearing BT-474 subcutaneous xenograft were intravenously injected with coumarin-6 loaded LTNPs (30 mg/kg) to study the targeting mechanisms in vivo.Results:The LTNPs particles were generally spherical but flexible under TEM and AFM, and approximately 62.1 nm in size with a zeta potential of 22.80 mV. In BT-474 cells, uptake of LTNPs was mediated by endosomes through energy-dependent endocytosis involving clathrin-dependent pinocytosis and macropinocytosis, and they could effectively escape from endosomes to the cytoplasm. Treatment of BT-474 cells with LTNPs (20 μg/mL) induced a significant cell arrest at G0/G1 phase compared with the same concentration of lapatinib suspension. In mice bearing BT-474 xenograft, intravenously injected LTNPs was found to target and accumulate in tumors, and colocalized with HER2 and SPRAC (secreted protein, acidic and rich in cysteine).Conclusion:LTNPs can be taken up into breast cancer cells through specific pathways in vitro, and targeted to breast cancer xenograft in vivo via enhanced permeability and retention effect and SPARC.
    Acta pharmacologica Sinica. 06/2014; 35(6):846-52.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective To establish a rapid eukaryotic expression system of hemagglutinin (HA) gene of novel avian influenza H7N9 using lentiviral vector, express the recombinant protein and study its functions in human embryonic kidney HEK293T cells. Methods The full-length HA gene was amplified from H7N9 genomic RNA by reverse transcription PCR (RT-PCR) and linked with pMD18-T vector to generate pMD18-T-HA plasmid. Blunt-end HA gene with Kozak sequence was amplified from pMD18-T-HA vector, and then pLenti-HA-V5 expression vector was constructed by Topo cloning for transient expression in HEK293T cells. Expression of HA-V5 recombinant protein was confirmed by immunofluorescence assay (IFA) and Western blotting. Hemagglutination test was performed to evaluate the biological activity of the recombinant protein. Results The full-length HA gene (1 683 bp) was obtained and eukaryotic expression plasmid was constructed successfully. A recombinant protein with relative molecular mass (Mr) 70 000 was expressed and the antigenicity and binding specificity to positive serum were demonstrated by IFA and Western blotting. The hemagglutination activity was proved by hemagglutination test. IFA and Western blotting showed that the Mr 70 000 recombinant protein had an immuoreactivity to positive serum. The hemagglutination activity was confirmed by hemagglutination test. Conclusion The rapid eukaryotic expression system of HA gene was successfully constructed, which laid a solid foundation for further research on subunit vaccine development, neutralizing epitope mapping and packaging pseudovirus.
    06/2014; 30(6):630-4.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The applications of inorganic nanomaterials as biomimetic catalysts are receiving much attention, because of their high stability and low cost. In this work, Co3O4 nanomaterials including nanoplates, nanorods and nanocubes were synthesized. The morphologies and compositions of the products were characterized by scanning electron microscopy, transmission electron microscopy and X-ray diffraction. The catalytic properties of Co3O4 nanomaterials as catalase mimics were studied. The Co3O4 materials with different morphology exhibited different catalytic activities in the order of nanoplates > nanorods > nanocubes. The difference of the catalytic activities originated from their different abilities of electron transfer. Their catalytic activities increased significantly in presence of calcium ion. Based on the stimulation by calcium ion, a biosensor was constructed by Co3O4 nanoplates for the determination of calcium ion. The biosensor had a linear relation to calcium concentrations and good measurement correlation between 0.1 and 1 mM with a detection limit of 4 µM (S/N=3). It showed high selectivity against other metal ions and good reproducibility. The proposed method was successfully applied for the determination of calcium in milk sample.
    ACS Applied Materials & Interfaces 05/2014; · 5.01 Impact Factor
  • Zemin Yao, Li Zhang, Guang Ji
    [Show abstract] [Hide abstract]
    ABSTRACT: There is an increasing interest and popularity of Chinese herbal medicine worldwide, which is accompanied by increasing concerns about its effectiveness and potential toxicity. Several ingredients, such as polyphenolic compounds berberine, flavonoids, and curcumin, have been studied extensively by using various animal models. Effectiveness of treatment and amelioration of metabolic syndromes, including insulin resistance and dyslipidemia, has been demonstrated. This review summarizes the major checkpoints and contributing factors in regulation of exogenous and endogenous lipid metabolism, with particular emphasis centered on triglyceride-rich and cholesterol-rich lipoproteins. Available experimental evidence demonstrating the lipid-lowering effect of berberine, flavonoids and curcumin in cell culture and animal models is compiled, and the strengths and shortcomings of experimental designs in these studies are discussed.
    Journal of integrative medicine. 05/2014; 12(3):135-146.
  • [Show abstract] [Hide abstract]
    ABSTRACT: The decomposition of carbon materials and organic binders in Li–air batteries has been reported repeatedly in recent literature. The decomposition of carbon can harm the batteries’ cyclability further by catalyzing electrolyte degrading. Therefore, there is a critical need to exploit a new catalyst support substituting carbon and develop a binder free cathode preparation strategy for Li–air batteries. Herein, TiO2 nanotube arrays growing on Ti foam are used as the catalyst support to construct carbon and binder free oxygen diffusion electrodes. After being coated with Pt nanoparticles by a cool sputtering approach, the TiO2 nanotube arrays are used as cathodes of Li–O2 batteries. Benefiting from the stability of TiO2 in the discharge/charge processes, the Li–O2 batteries realize enhanced cyclability at high current densities (for instance, more than 140 cycles at 1 or 5 A g–1), within wide discharge/charge voltage windows (for instance, 1.5–4.5 V). X-ray photoelectron spectra and a scanning electron microscope image of the cathodes after cycling at 5 A g–1 150 times indicate that the TiO2 nanotubes can remain stable in the long term cycle test. 1H nuclear magnetic resonance analysis reveals that the tetraethylene glycol dimethyl ether electrolyte has no degradation, showing enhanced stability compared with that in the carbon containing batteries.
    Chemistry of Materials. 04/2014; 26(8):2551–2556.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives. Published association studies of killer cell immunoglobulin-like receptors (KIRs) and ankylosing spondylitis (AS) in populations are inconsistent. The aim of this study is to determine whether the KIR polymorphisms confer susceptibility to AS in populations by conducting a meta-analysis. Methods. A computer search was carried out up to August 2013 for literature pertaining to AS and KIR polymorphisms. Publications addressing the association between the KIR polymorphisms and susceptibility to AS in populations were selected from the Pubmed, Elsevier Science Direct, China National Knowledge Infrastructure (CNKI) and Chinese Biomedical Literature Database (CBM) databases. The odds ratio (OR) with 95% confidence interval (95%CI) was calculated. Results. A total of 13 case-control studies in 9 articles were included in this meta-analysis. Meta-analysis results identified two positive associations of 2DS4 and 3DS1 with susceptibility to AS in populations. In subgroup analysis, there was a positive association between 2DS4 and susceptibility to AS in Asians, but not in Caucasians. And there were associations between 3DL1, 3DS1 and susceptibility to AS in Caucasians, but not in Asians. Results of subgroup analysis also showed that there were associations between 2DL5, 2DS4, 2DS5, 3DL1, 3DS1 and susceptibility to AS in HLA-B*27-positive patients and HLA-B*27-positive healthy controls. Conclusions. This meta-analysis confirms that 2DS4 and 3DS1 might be potential risk factors for AS in populations.
    Modern Rheumatology 03/2014; · 1.72 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Src-suppressed C kinase substrate (SSeCKS), an in vivo and in vitro protein kinase C substrate, is a major lipopolysaccharide (LPS) response protein which markedly upregulated in several organs, including brain, lung, heart, kidney etc., indicating a possible role of SSeCKS in inflammatory process. However, the expression and biological function of SSeCKS during neuronal inflammation remains to be elucidated, so we established an inflammatory model injected with LPS to investigate the gene expression patterns of SSeCKS in neural tissues by using TaqMan quantitative real-time PCR and immunohistochemistry in rat. Real-time PCR showed that LPS stimulated the expression of SSeCKS mRNA in a dose- and time-dependent manner in sciatic nerves, spinal cords and dorsal root ganglions. Immunohistochemistry showed that SSeCKS colocalized with nerve fibers in sciatic nerve after LPS administration, but there was no colocalization between SSeCKS and Schwann cells. In addition, SSeCKS colocalized with neurons which existed in dorsal root ganglions and spinal cords. These findings indicated that SSeCKS might play some important roles in sciatic nerve fibers and neurons in spinal cords and dorsal root ganglions after LPS injection.
    Neurochemical Research 03/2014; · 2.13 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Grating-based x-ray imaging systems are of two categories: interferometric and non-interferometric. This work focuses on the angular sensitivity of the phase-stepping-based non-interferometric setup. First, a numerical model of the system is developed and verified with experimental results. Then, an existing system is optimized and verified by comparison with biological sample experiments. The results are also compared with those of a Talbot-Lau interferometer. Finally, an analytical formula of the system sensitivity is derived and limitations of the setup are discussed.
    Physics in Medicine and Biology 03/2014; 59(7):1573-1588. · 2.70 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Fever is a prominent feature of diseases and is an ongoing process that is always accompanied by metabolic changes in the body system. Despite the success of temperature regulation theory, the underlying biological process remains unclear. To truly understand the nature of the febrile response, it is crucial to confirm the biomarkers during the entire biological process. In the current study, a 73-h metabolic footprint analysis of the urine from yeast-induced pyrexia rats was performed using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Potential biomarkers were selected using orthogonal partial least squares-discriminate analysis (OPLS-DA), the rational biomarkers were verified by Pearson correlation analysis, and the predictive power was evaluated using receiver operator characteristic (ROC) curves. A metabolic network constructed using traditional Chinese medicine (TCM) grammar systems was used to validate the rationality of the verified biomarkers. Finally, five biomarkers, including indoleacrylic acid, 3-methyluridine, tryptophan, nicotinuric acid and PI (37:3), were confirmed as rational biomarkers because their correlation coefficients were all greater than 0.87 and because all of the correlation coefficients between any pair of these biomarkers were higher than 0.75. The areas under the ROC curves were all greater than 0.84, and their combined predictive power was considered reliable because the greatest area under the ROC curve was 0.968. A metabolic network also demonstrated the rationality of these five biomarkers. Therefore, these five metabolites can be adopted as rational biomarkers to reflect the process of the febrile response in inflammation-induced pyrexia.
    Journal of pharmaceutical and biomedical analysis 02/2014; 95C:68-75. · 2.45 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Mesenchymal stem cells (MSCs) have both multi-lineage differentiation potential and immunosuppressive properties, making them ideal candidates for regenerative medicine. However, their immunosuppressive properties potentially increase the risk of cancer progression and opportunistic infections. In this study, MSCs isolated from human umbilical cord blood (UCMSCs) and adult bone marrow (BMMSCs) were infected with human cytomegalovirus (HCMV). Cytopathic changes were observed 10 days post infection. PCR products amplified from genomic DNA and cDNA were used to confirm the HCMV infection of the UCMSCs and BMMSCs. Real-time PCR was conducted to quantify the expression of immunomodulatory molecules, including cytokines, chemokines, growth factors, adhesion molecules and cancer-related genes. Our results indicate high upregulation of the majority of these molecules, including many growth factors, tumor necrosis factor alpha, interleukin-8, interleukin-6 and interferon gamma. Adhesion molecules (VCAM-1, TCAM-1 and selectin-E) were downregulated in the infected UCMSCs and BMMSCs. Antibody chip array evaluation of cell culture media indicated that the growth factor secretion by UCMSCs and BMMSCs was greatly influenced (p < 0.001) by HCMV. The stimulation of MSCs with HCMV led to the activation of downstream signaling pathways, including pSTAT3 and Wnt2. Our results show that HCMV can significantly alter the functions of both UCMSCs and BMMSCs, although not in the same way or to the same extent. In both cases, there was an increase in the expression of proangiogenic factors in the microenvironment following HMCV infection. The discrepancy between the two cell types may be explained by their different developmental origin, although further analysis is necessary. Future studies should decipher the underlying mechanism by which HCMV controls MSCs, which may lead to the development of new therapeutic treatments.
    Cellular & Molecular Biology Letters 02/2014; · 1.95 Impact Factor

Publication Stats

2k Citations
676.69 Total Impact Points

Institutions

  • 2014
    • Beijing University of Chinese Medicine and Pharmacology
      • School of Chinese Materia Medica
      Peping, Beijing, China
    • Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
      Hua-yang, Sichuan, China
    • Jiangsu Provincial Center for Disease Control and Prevention
      Chiang-tu, Jiangsu Sheng, China
    • Nanjing Medical University
      Nan-ching, Jiangsu Sheng, China
  • 2012–2014
    • Soochow University (PRC)
      • Department of Materials Science and Engineering
      Wu-hsien, Jiangsu Sheng, China
    • Harbin Institute of Technology
      • Academy of Fundamental and Interdisciplinary Science
      Charbin, Heilongjiang Sheng, China
    • Capital Medical University
      Peping, Beijing, China
  • 2011–2014
    • Peking Union Medical College Hospital
      Peping, Beijing, China
    • East China Normal University
      • Institute of Biomedical Sciences and School of Life Sciences
      Shanghai, Shanghai Shi, China
    • Tianjin University of Traditional Chinese Medicine
      T’ien-ching-shih, Tianjin Shi, China
  • 2010–2014
    • Shanghai University of Traditional Chinese Medicine
      • Institute of Chinese Materia Medica
      Shanghai, Shanghai Shi, China
    • Lanzhou University
      Kao-lan-hsien, Gansu Sheng, China
  • 2009–2014
    • Suzhou University
      • Department of Chemistry
      Suchow, Anhui Sheng, China
    • Shanghai Institute of Measurement and Testing Technology
      Shanghai, Shanghai Shi, China
    • U.S. Food and Drug Administration
      • Center for Drug Evaluation and Research
      Washington, D. C., DC, United States
    • Zhejiang Medical University
      Hang-hsien, Zhejiang Sheng, China
  • 2008–2014
    • Nantong University
      Tungchow, Jiangsu Sheng, China
  • 2004–2014
    • Tsinghua University
      • Department of Engineering Physics
      Peping, Beijing, China
  • 2002–2014
    • Sichuan University
      • • Key Laboratory of Drug Targeting and Novel Drug Delivery System
      • • Analytical Center
      • • Department of Urology
      • • Department of Cardiology
      • • Department of Pediatrics
      • • Laboratory of Transplant Engineering and Immunology
      Hua-yang, Sichuan, China
  • 2013
    • Harbin Normal University
      Charbin, Heilongjiang Sheng, China
  • 2012–2013
    • Anhui Medical University
      • Department of Epidemiology and Biostatistics
      Hefei, Anhui Sheng, China
  • 2006–2013
    • Anhui University
      • • School of Life Sciences
      • • School of Chemistry and Chemical Engineering
      Luchow, Anhui Sheng, China
    • Hospital Universitario de Maracaibo
      Maracaibo, Estado Zulia, Venezuela
  • 2009–2012
    • Nantong Medical College
      • • Department of Microbiology and Immunology
      • • Department of Pathology
      Tungchow, Jiangsu Sheng, China
  • 2008–2012
    • China Agricultural University
      • Department of Applied Chemistry
      Beijing, Beijing Shi, China
  • 2009–2011
    • Fourth Military Medical University
      Xi’an, Liaoning, China
  • 2008–2011
    • IBM
      Armonk, New York, United States
  • 2007–2011
    • University of Florida
      • • Department of Medicine
      • • Division of Nephrology, Hypertension & Renal Transplantation
      Gainesville, Florida, United States
  • 2003–2009
    • Baylor College of Medicine
      • Program in Developmental Biology
      Houston, Texas, United States