Li Zhang

Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Hua-yang, Sichuan, China

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Publications (503)862.6 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Abstract To overcome multidrug resistance (MDR) in cancer chemotherapy with high efficiency and safety, a reduction-sensitive liposome (CL-R8-LP), which was co-modified with reduction-sensitive cleavable PEG and octaarginine (R8) to increase the tumor accumulation, cellular uptake and lysosome escape, was applied to co-encapsulate doxorubicin (DOX) and a P-glycoprotein (P-gp) inhibitor of verapamil (VER) in this study. The encapsulation efficiency (EE) of DOX and VER in the binary-drug loaded CL-R8-LP (DOX + VER) was about 95 and 70% (w/w), respectively. The uptake efficiencies, the cytotoxicity, and the apoptosis and necrosis-inducing efficiency of CL-R8-LP (DOX + VER) were much higher than those of DOX and the other control liposomes in MCF-7/ADR cells or tumor spheroids. Besides, CL-R8-LP (DOX + VER) was proven to be uptaken into MCF-7/ADR cells by clathrin-mediated and macropinocytosis-mediated endocytosis, followed by efficient lysosomal escape. In vivo, CL-R8-LP (DOX + VER) effectively inhibited the growth of MCF-7/ADR tumor and reduce the toxicity of DOX and VER, which could be ascribed to increased accumulation of drugs in drug-resistant tumor cells and reduced distribution in normal tissues. In summary, the co-delivery of chemotherapeutics and P-gp inhibitors by our reduction-sensitive liposome was a promising approach to overcome MDR, improve anti-tumor effect and reduce the toxicity of chemotherapy.
    Drug delivery. 12/2014;
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    ABSTRACT: Autophagy is a lysosomal degradation pathway that protects cancer cells from multiple endogenous and extraneous stresses, particularly during the pathogenesis of cancer. An autophagic balance exists in the tumour microenvironment. Appropriate disturbance to this balance may have therapeutic potential. Here, we report a novel antitumour strategy based on an autophagic catastrophic vacuolisation effect in tumour cells. We achieved this effect via initiative induction and the terminal restraint of autophagic flux. The TAT-Beclin 1 peptide (T-B) was constructed for the initiative induction of autophagic flux, whereas hydroxychloroquine (HCQ)-loaded liposomes (HCQ-Lip) were constructed for terminal restraint. We demonstrate that T-B, a new CPP tandem autophagy inducing peptide, effectively activates the autophagy signal at the early stage of the autophagy pathway. HCQ deacidified the lysosome during the final stage of autophagy. We combined T-B and HCQ-Lip to induce autophagic catastrophic vacuolisation and death in several tumour cell lines based on the idea of "broadening sources of income and reducing expenditure". The co-treated group exhibited at least a 1.86-fold greater and up to 5.66-fold greater cytotoxic effect in vitro. In addition, this strategy showed at least a 2.0-fold tumour inhibitory effect compared to the other groups in vivo. Therefore, this bidirectional accumulation of autophagic vesicles exhibited potential efficacy for tumour treatment. Copyright © 2014. Published by Elsevier B.V.
    Journal of controlled release : official journal of the Controlled Release Society. 12/2014;
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    ABSTRACT: A global-chemical-profiling-based quality evaluation approach by using ultra performance liquid chromatography with tandem quadrupole time-of-flight mass spectrometry was developed for the quality evaluation of three rhubarb species, including Rheum palmatum L., R. tanguticum Maxim. ex Balf. and R. officinale Baill. Considering that comprehensive detection of chemical component is crucial for the global profile, a systemic column performance evaluation method was developed. Based on this, Cortecs column was used to acquire the chemical profile, and Chempattern software was employed to conduct Similarity evaluation and Hierarchical cluster analysis. The results showed R. tanguticum could be differentiated from R. palmatum and R. officinale at the similarity value 0.65, but R. palmatum and R. officinale could not be distinguished effectively. Therefore, the common pattern based on three rhubarb species was developed to conduct the quality evaluation, and the similarity value 0.50 was set as an appropriate threshold to control the quality of rhubarb. A total of 88 common peaks were identified by their accurate mass and fragmentations, and partially verified by reference standards. Through the verification, the newly developed method could be successfully used for evaluating the holistic quality of rhubarb. It would provide a reference for the quality control of other herbal medicines. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
    Journal of Separation Science 12/2014; · 2.59 Impact Factor
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    ABSTRACT: RuO2 modified TiO2 nanotube arrays, growing on Ti foams, are used as carbon and binder free cathodes for Li–O2 batteries. The micrometer pores in Ti foams and nanometer pores in TiO2 nanotubes supply facilitated transport channels for oxygen diffusing into/out of the catalysts in discharge and charge processes. The RuO2 catalyst exhibits outstanding catalytic active toward oxygen evolution reaction (OER), which leads the charge voltage maintaining around 3.7 V all through the battery cycling. The stability of TiO2/Ti support, abundant oxygen transport path and favorable catalytic activity of RuO2 toward OER enable the Li–O2 batteries exhibiting 130 cycle discharge/charge.
    Journal of Power Sources 12/2014; 270:386–390. · 5.26 Impact Factor
  • 14 AIChE Annual Meeting; 11/2014
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    ABSTRACT: Disease-specific humoral immune response-related protein complexes in blood are associated with disease progression. Thirty-one patients with stage IIIB and IV non-small-cell lung cancer (NSCLC) were administered with oral dose of icotinib hydrochloride (150mg twice daily or 125mg 3 times daily) for a 28-continuous-day cycle until diseases progressed or unacceptable toxicity occurred. The levels of immunoinflammation-related protein complexes (IIRPCs) in a series of plasma samples from 31 NSCLC patients treated with icotinib hydrochloride were determined by an optimized native polyacrylamide gel electrophoresis. Three characteristic patterns of the IIRPCs, named as patterns a, b, and c, respectively, were detected in plasma samples from 31 patients. Prior to the treatment, there were 18 patients in pattern a consisting of 5 IIRPCs, 9 in pattern b consisting of six IIRPCs, and 4 in pattern c without the IIRPCs. The levels of the IIRPCs in 27 patients were quantified. Our results indicate that the time length of humoral immune and inflammation response (TLHIIR) was closely associated with disease progression, and the median TLHIIR was 22.0weeks, 95% confidence interval: 16.2 to 33.0weeks, with a lead time of median 11weeks relative to clinical imaging evidence confirmed by computed tomography or magnetic resonance imaging (the median progression-free survival, 34.0weeks, 95% confidence interval: 27.9 to 49.0weeks). The complex relationships between humoral immune response, acquired resistance, and disease progression existed. Personalized IIRPCs could be indicators to monitor the disease progression. Copyright © 2014 Elsevier B.V. All rights reserved.
    11/2014; 440C:44-48.
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    ABSTRACT: The roles of microRNAs (miRNAs) in the regulation of metastasis have been widely recognized in the recent years. Mir-10b antagomir (antagomir-10b) was shown to impede metastasis through the down-regulation of mir-10b; however, it could not stunt the growth of primary tumors. In this study we showed that the co-delivery of antagomir-10b with paclitaxel (PTX) by a novel liposomal delivery system modified with an anti-microbial peptide [D]-H6L9 (D-Lip) could significantly both hinder the migration of 4T1 cells and induce evident cellular apoptosis and cell death in the meantime. The histidines in the sequence of [D]-H6L9 allowed the peptide to get protonated under pH5.0 (mimicking the lysosome/endosome environment), and strong membrane lytic effect could thus be activated, leading to the escape of liposomes from the lysosomes and the decrease of of mir-10b expression. The in vivo and ex vivo fluorescence imaging showed that D-Lip could reach 4T1 tumors efficaciously. Incorporation of PTX did not influence the antagomir-10b delivery effect of D-Lip; for the in vivo tumor inhibition assay, compared with all the other groups, the combination of antagomir-10b and PTX delivered by D-Lip could prominently delay the growth of 4T1 tumors and reduce the lung metastases at the same time, and the expression of Hoxd10 in tumors was also significantly up-regulated. Taken together, these results demonstrated that D-Lip could act as a sufficient tool in co-delivering antagomir-10b and PTX. Copyright © 2014. Published by Elsevier B.V.
    Journal of controlled release : official journal of the Controlled Release Society. 11/2014;
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    ABSTRACT: To combine the advantage of poly(ethylene gylcol) (PEG) for longer circulation and cell-penetrating peptides (CPPs) for efficient cellular uptake, paclitaxel (PTX)-loaded liposomes functionalized with TAT, the most frequently used CPP, and cleavable PEG via a redox-responsive disulfide linker (PTX-C-TAT-LP) were successfully developed here. Under physiological conditions, TAT was shielded by PEG layer and liposomes exhibited a long blood circulation. At tumor site, PEG could be detached in the presence of exogenous reducing agent [glutathione (GSH)] and TAT was exposed to facilitate cell internalization. In the presence of GSH, the liposomal vesicle C-TAT-LP showed increased cellular uptake and improved three-dimensional tumor spheroids penetration in vitro compared with analogous stable shielded liposomes. C-TAT-LP achieved enhanced tumor distribution and demonstrated superior delivery efficiency in vivo. PTX-C-TAT-LP with GSH strongly inhibited the proliferation of murine melanoma B16F1 tumor cells in vitro and in vivo with the tumor inhibition rate being 69.4% on B16F1-bearing mice. In addition, the serum aspartate transaminase level, alanine transaminase level, and creatine kinase level were almost completely within normal range in the PTX-C-TAT-LP with GSH group, revealing PTX-C-TAT-LP with GSH had no obvious drug-related adverse events for liver and heart. Taken together, C-TAT-LP is a promising tumor-targeting drug carrier. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci
    Journal of Pharmaceutical Sciences 11/2014; · 3.13 Impact Factor
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    ABSTRACT: Glioma is still hard to be treated due to their complex microenvironment. In this study, a gold nanoparticle-based delivery system was developed. The system, An-PEG-DOX-AuNPs, was loaded with doxorubicin (DOX) through hydrazone, an acid-responsive linker, and was functionalized with angiopep-2, a specific ligand of low density lipoprotein receptor-related protein-1 (LRP1), which could mediate the system to penetrate blood brain barrier and target to glioma cells. The particle size of An-PEG-DOX-AuNPs was 39.9 nm with a zeta potential of -19.3 mV, while the DOX loading capacity was 9.7%. In vitro, the release of DOX from DOX-AuNPs was pH-dependent. At lower pH values, especially 5.0 and 6.0, release of DOX was much quicker than that at pH 6.8 and 7.4. After coating with PEG, the acid-responsive release of DOX from PEG-DOX-AuNPs was almost the same as that from DOX-AuNPs. Cellular uptake study showed obviously higher intensity of intracellular An-PEG-DOX-AuNPs compared with PEG-DOX-AuNPs. In vivo, An-PEG-DOX-AuNPs could distribute into glioma at a higher intensity than that of PEG-DOX-AuNPs and free DOX. Correspondingly, glioma-bearing mice treated with An-PEG-DOX-AuNPs displayed the longest median survival time, which was 2.89-fold longer than that of saline. In conclusion, An-PEG-DOX-AuNPs could specifically deliver and release DOX in glioma and significantly expand the median survival time of glioma-bearing mice. Copyright © 2014 Elsevier Ltd. All rights reserved.
    Biomaterials 10/2014; 37C:425-435. · 8.31 Impact Factor
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    ABSTRACT: Signal transducer and activator of transcription 3 (STAT3) is implicated breast cancer metastasis and represents a potential target for developing new anti-tumor metastasis drugs. The purpose of this study is to investigate whether the natural agent 1'-acetoxychavicol acetate (ACA), derived from the rhizomes and seeds of Languas galanga, could suppress breast cancer metastasis by targeting STAT3 signaling pathway. ACA was examined for its effects on breast cancer migration/invasion and metastasis using Transwell assays in vitro and breast cancer skeletal metastasis mouse model in vivo (n = 10 mice per group). The inhibitory effect of ACA on cellular STAT3 signaling pathway was investigated by series of biochemistry analysis. The chavicol preferentially suppressed cancer cell migration and invasion, and this activity was superior to its cytotoxic effects. ACA suppressed both constitutive and interleukin-6-inducible STAT3 activation and diminished the accumulation of STAT3 in the nucleus and its DNA-binding activity. More importantly, ACA treatment led to significant up-regulation of Src homology region 2 domain-containing phosphatase 1 (SHP-1), and the ACA-induced depression of cancer cell migration and STAT3 signaling could be apparently reversed by blockade of SHP-1. Matrix metalloproteinase (MMP)-2 and -9, gene products of STAT3 that regulate cell invasion, were specifically suppressed by ACA. In tumor metastasis model, ACA potently inhibited the human breast cancer cell-induced osteolysis, and had little apparent in vivo toxicity at the test concentrations. ACA is a novel drug candidate for the inhibition of tumor metastasis through interference with the SHP-1/STAT3/MMPs signaling pathway.
    Breast Cancer Research and Treatment 10/2014; · 4.47 Impact Factor
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    ABSTRACT: Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of metabolic syndrome and is one of the most prevalent liver disorders worldwide. NAFLD can gradually progress to liver inflammation, fibrosis, cirrhosis and even hepatocellular carcinoma. However, the pathogenesis of NAFLD is complex, and no efficient pharmaceutic treatments have yet been established for NAFLD. Accumulating data have shown that the farnesoid X receptor (FXR) plays important roles not only in bile acid metabolism, but also in lipid and carbohydrate homeostasis, inflammatory responses, among others. In this review, we aim to highlight the role of FXR in the pathogenesis and treatment of NAFLD.
    World journal of gastroenterology : WJG. 10/2014; 20(37):13493-13500.
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    ABSTRACT: Please see the attached file.
    J. Mater. Chem. B. 10/2014;
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    ABSTRACT: Silicon offers extremely high theoretical specific capacity, but suffers from dramatic volume change during lithiation/delithiation processes, which typically leads to rapid anode degradation. Here we designed a facile and self-assembly strategy to construct three-dimensional (3D) polymeric network as a promising binder for high-performance silicon submicro-particle (SiSMPs) anodes through in-situ interconnecting alginate chains by additive divalent cations. The highly cross-linked alginate network allows superb mechanical property and strong interactions with SiSMPs, which can tolerate the volume change of SiSMPs and restrict the volume expansion of the laminates to a large degree, thus effectively maintaining the mechanical and electrical integrity of the electrode and significantly improving the electrochemical performance. As a result, SiSMPs with 3D binder network exhibit high reversible capacity, superior rate capability and much prolonged cycle life. Additionally, the 3D alginate binder was also successfully applied for the industrial submicro-silicon waste from solar cell production. With all of the advantages, the innovative way to tolerate the severe volume change by using high-strength polymeric network may open a new approach to realize the industrial application of Si-based anode into lithium-ion batteries.
    J. Mater. Chem. A. 09/2014;
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    ABSTRACT: To examine the changes in expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) protein, p-PTEN protein and Bim (Bcl-2 interacting mediator of cell death) mRNA in the cortex of neonate rat brains with hypoxic-ischemic brain damage (HIBD) and to explore the mechanisms of neuroprotective effects of PTEN inhibition.
    09/2014; 45(5):767-71.
  • Applied Catalysis B Environmental 09/2014; s 156–157:371–377. · 5.83 Impact Factor
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    ABSTRACT: Background Salvia-Nelumbinis naturalis (SNN), initially called Jiangzhi Granula as a formulae of Chinese medicinal decoction, has been used clinically to treat non-alcoholic fatty liver disease (NAFLD) and related syndromes. The mechanism of SNN action is unknown.Methods HepG2 cells were cultured in lipid-rich media supplemented with chemical components of SNN. Male Wistar rats (6 weeks of age) were fed a high calorie diet (15% fat, 15% sucrose, and 2% cholesterol) for eight weeks, and then treated with SNN for four weeks. Body and liver weight, lipids profiles, insulin and glucose levels, glucose and insulin tolerance were evaluated, the mRNA and protein expression of insulin receptor (InsR), insulin receptor substrate (IRS) 1/2, protein kinase B (PKB/Akt), protein expression of suppressor of cytokine signaling 3 (SOCS3), protein kinase C epsilon (PKC ¿) in liver tissue were analysed.ResultsTreatment with SNN components in lipid-laden HepG2 cells decreased lipid accumulation. Rats fed with a HC diet developed hepatosteatosis and accompanied hyperglycemia, hyperinsulinemia, hyperleptinemia, and diabetic dyslipidemia. Prolonged HC diet feeding resulted in parabolic response in plasma triglyceride (TG) concentrations, indicative of compromised hepatic production of TG-rich lipoproteins. HC diet feeding also resulted in impaired insulin sensitivity and hepatic insulin signalling. Administration of SNN extracts alleviated hepatosteatosis and conferred to a normolipoproteinemia profile in the HC diet-fed rats. The efficacy of SNN extract in improving liver function and insulin sensitivity was comparable to that of simvastatin or pioglitazone. The improved insulin signaling by SNN treatment was associated with increased IRS and Akt phosphorylation and decreased SOCS3 expression. However, SNN failed to inhibit the PKC ¿ expression in the liver.ConclusionsSNN is effective in reducing lipid accumulation in HepG2 cells and attenuating hepatosteatosis in HC diet-fed rats. Reduced hepatic lipid content in the rat liver was associated with improved insulin signalling.
    Journal of translational medicine. 08/2014; 12(1):236.
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    ABSTRACT: Ultra-high loading Co3O4 nano-particles anchoring onto the acid-sonication pretreated graphene oxide with induced in-plane vacancies were applied as the high-performance lithium-ion anodes.
    Electrochimica Acta 08/2014; 136:330–339. · 4.09 Impact Factor
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    ABSTRACT: The Chinese medicinal formula, Qinggan (QG) Huoxue (HX) Recipe (R) exerts a range of pharmacological effects, including reversible steatosis, decreased levels of inflammatory cytokines and lipid peroxidation resistance. The aim of the present study was to determine the specific mechanisms of QGHXR hepatoprotection through the lipopolysaccharide-Kupffer cell (LPS-KC) signal conduction pathway in rats with alcoholic liver disease (ALD). ALD rats were exposed to the compound factors, QGR and HXR. Hematoxylin and eosin staining was conducted to evaluate the pathological changes in the liver following QGHXR treatment and an enzyme-linked immunosorbent assay was performed to measure the content of tumor necrosis factor (TNF)-α in the plasma. Immunohistochemical staining was conducted to examine the expression of cell differentiation antigen (CD) 68 and 14. In addition, western blot analysis and reverse transcription-polymerase chain reaction were used to measure the expression of Toll-like receptor 4 (TLR4), phosphorylated-extracellular regulated protein kinases (p-ERK), nuclear factor (NF)-κB, CD14 and TNF-α. Following stimulation with the compound factors, the rats exhibited increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as marked pathological changes. Furthermore, the related molecules in the LPS-KC pathway were upregulated and QGHXR was identified to be effective in the LPS-KC signal conduction pathway in the ALD rats. QGHXR was superior to QGR and HXR in reducing the serum ALT and AST levels, regulating CD14, TLR4, NF-κB, ERK and TNF-α as well as improving the pathological changes. The results indicated that QGHXR therapy may provide a novel strategy for treating ALD via regulation of the related molecules in the LPS-KC signaling pathway.
    Experimental and therapeutic medicine 08/2014; 8(2):363-370. · 0.34 Impact Factor
  • Catalysis Today 08/2014; 231:64–74. · 3.31 Impact Factor
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    ABSTRACT: Mesenchymal stem cells (MSCs) have the potential to differentiate into several cell types and provide an attractive source of autologous cells for regenerative medicine. However, their cellular biology is not fully understood. Similar to Ca(2+), extracellular Mg(2+) plays an important role in the functions of the skeletal system. Here, we examined the effects of extracellular Mg(2+) on the deposition of calcium phosphate matrix and Ca(2+) signaling with or without ATP stimulation in human bone marrow-derived mesenchymal stem cells (hBMSCs). We found that high extracellular Mg(2+) concentration ([Mg(2+)]e) inhibited extracellular matrix mineralization in hBMSCs in vitro. hBMSCs also produced a dose-dependent decrease in the frequency of calcium oscillations during [Mg(2+)]e elevation with a slight suppression on oscillation amplitude. In addition, spontaneous ATP release was inhibited under high [Mg(2+)]e levels and exogenous ATP addition stimulated oscillation reappear. Taken together, our results indicate that high [Mg(2+)]e modulates calcium oscillations via suppression of spontaneous ATP release and inactivates purinergic receptors, resulting in decreased extracellular mineralized matrix deposition in hBMSCs. Therefore, the high magnesium environment created by the rapid corrosion of Mg alloys may result in the dysfunction of calcium-dependent physiology processes and be disadvantageous to hBMSCs physiology.
    Biochemical and Biophysical Research Communications 07/2014; · 2.28 Impact Factor

Publication Stats

2k Citations
862.60 Total Impact Points

Institutions

  • 2014
    • Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
      Hua-yang, Sichuan, China
    • Southwest University
      Kenner, Louisiana, United States
    • Beijing University of Chinese Medicine and Pharmacology
      • School of Chinese Materia Medica
      Peping, Beijing, China
    • Nanjing Medical University
      Nan-ching, Jiangsu Sheng, China
    • Jiangsu Provincial Center for Disease Control and Prevention
      Chiang-tu, Jiangsu Sheng, China
  • 2013–2014
    • University of Houston
      • Department of Chemical & Biomolecular Engineering
      Houston, Texas, United States
    • Jilin University
      • The First Clinical Hospital
      Yung-chi, Jilin Sheng, China
    • Harbin Normal University
      Charbin, Heilongjiang Sheng, China
  • 2012–2014
    • Soochow University (PRC)
      • Department of Materials Science and Engineering
      Wu-hsien, Jiangsu Sheng, China
    • Harbin Institute of Technology
      • Academy of Fundamental and Interdisciplinary Science
      Charbin, Heilongjiang Sheng, China
    • Beijing Information Science and Technology University
      Peping, Beijing, China
    • Wuhan Institute Of Virology
      Wu-han-shih, Hubei, China
  • 2011–2014
    • Capital Medical University
      • • Beijing Center for Disease Prevention and Control (CDC)
      • • School of Public Health and Family Medicine
      Peping, Beijing, China
    • Peking Union Medical College Hospital
      Peping, Beijing, China
    • East China Normal University
      • Institute of Biomedical Sciences and School of Life Sciences
      Shanghai, Shanghai Shi, China
    • Tianjin University of Traditional Chinese Medicine
      T’ien-ching-shih, Tianjin Shi, China
  • 2010–2014
    • Shanghai University of Traditional Chinese Medicine
      • Institute of Chinese Materia Medica
      Shanghai, Shanghai Shi, China
    • Beijing Centers for Disease Control and Prevention
      Peping, Beijing, China
    • Hefei University of Technology
      Luchow, Anhui Sheng, China
    • Lanzhou University
      Kao-lan-hsien, Gansu Sheng, China
  • 2008–2014
    • Nantong University
      Tungchow, Jiangsu Sheng, China
  • 2004–2014
    • Tsinghua University
      • Department of Engineering Physics
      Peping, Beijing, China
  • 2002–2014
    • Sichuan University
      • • Key Laboratory of Drug Targeting and Novel Drug Delivery System
      • • Analytical Center
      • • Department of Urology
      • • Department of Cardiology
      • • Department of Pediatrics
      • • Laboratory of Transplant Engineering and Immunology
      Hua-yang, Sichuan, China
  • 2012–2013
    • Anhui Medical University
      • Department of Epidemiology and Biostatistics
      Hefei, Anhui Sheng, China
  • 2010–2013
    • University of Science and Technology of China
      • Department of Chemistry
      Luchow, Anhui Sheng, China
  • 2008–2013
    • Southwest University in Chongqing
      • • School of Psychology
      • • School of Chemistry and Chemical Engineering
      Chongqing, Chongqing Shi, China
  • 2009–2012
    • Wuhan University
      • • State Key Laboratory of Virology
      • • Department of Immunology
      Wuhan, Hubei, China
    • Nantong Medical College
      • • Department of Microbiology and Immunology
      • • Department of Pathology
      Tungchow, Jiangsu Sheng, China
    • Zhejiang Medical University
      Hang-hsien, Zhejiang Sheng, China
    • U.S. Food and Drug Administration
      • Center for Drug Evaluation and Research
      Washington, D. C., DC, United States
    • Shanghai Institute of Measurement and Testing Technology
      Shanghai, Shanghai Shi, China
  • 2008–2012
    • China Agricultural University
      • Department of Applied Chemistry
      Beijing, Beijing Shi, China
  • 2009–2011
    • Fourth Military Medical University
      Xi’an, Liaoning, China
  • 2008–2011
    • IBM
      Armonk, New York, United States
  • 2007–2011
    • University of Florida
      • • Department of Medicine
      • • Division of Nephrology, Hypertension & Renal Transplantation
      Gainesville, Florida, United States
    • Beijing Normal University
      Peping, Beijing, China
  • 2006–2011
    • Beijing Jiaotong University
      Peping, Beijing, China
    • Hospital Universitario de Maracaibo
      Maracaibo, Estado Zulia, Venezuela
  • 2000–2009
    • Baylor College of Medicine
      • Program in Developmental Biology
      Houston, Texas, United States