Li Wang

Sun Yat-Sen University Cancer Center, Shengcheng, Guangdong, China

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Publications (228)406.77 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Glioma stem cells (GSCs) are believed to contribute to glioblastoma multiforme (GBM) propagation and treatment resistance. Tanshinone IIA possesses anticancer and anti-inflammatory activities. This study aimed to determine the inhibitory effect of tanshinone IIA on human GSCs in vitro and in vivo and to explore the underlying mechanisms. In the present study, human GBM neurospheres (GBMS) were isolated from adherent GBM cells in serum-free medium, and the cells from the GBMS displayed characteristics of GSCs. Results from the MTT, neurosphere formation and in vivo inhibition assays revealed that tanshinone IIA had a significant inhibitory effect on human GSCs in vitro and in vivo. Furthermore, tanshinone IIA increased the expression of differentiation and neural lineage markers including GFAP and β-tubulin, decreased expression of GSC markers including CD133 and nestin, and induced GSC apoptosis in vitro and in vivo in a dose‑dependent manner. Inflammatory cytokines and signaling pathways are believed to play key roles in maintaining the stem-like properties in human glioma cells. In the present study, inflammatory cytokine interleukin 6 (IL6) and its downstream activated signal transducer and activator of transcription 3 [phospho-STAT3(tyrosine705) and phospho-STAT3(serine727)] were downregulated after tanshinone IIA treatment in vitro and in vivo. This result indicated that disturbance of the IL6/STAT3 signaling axis by tanshinone IIA is closely related to the growth inhibition of GSCs. Taken together, our results indicate that tanshinone IIA has the potential to target and kill GSCs through suppression of proliferation, attenuation of stemness and induction of apoptosis. Its mechanism of activity may be associated with attenuation of the IL6/STAT3 signaling pathway.
    Oncology reports. 06/2014;
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    ABSTRACT: Knowledge is limited about the relationship between chemotherapy and prognosis among the subtypes of axillary node-negative breast cancer (ANNBC). In this study, a population including 2,236 primary and operable ANNBC patients, with a median age of 53, were included. All breast tumors were classified into five immunohistochemically defined subtypes-luminal A, luminal B, luminal human epidermal growth factor receptor 2 (HER2), HER2 overexpression, and triple negative. With a median follow-up of 73.6 months, the rate of relapse was lowest in luminal A (6.5 %) and highest in HER2 overexpression subtype (16.4 %). Multivariate analysis indicated that the risks of relapse and death were enhanced in HER2 overexpression and triple-negative (TN) subtypes, and these two subtypes were independent predictors of relapse and death. Luminal A patients with risk factors could benefit from chemotherapy in terms of relapse-free survival (RFS). The relapse rate of TN patients after chemotherapy with taxanes was lower compared with that after chemotherapy without taxanes. In conclusion, women with ANNBC were at higher risks of relapse and death if suffering from HER2 overexpression or TN diseases. Chemotherapy could reduce the recurrence rate of luminal A patients with risk factors. TN patients may benefit from adjuvant chemotherapy containing taxanes.
    Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 05/2014;
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    ABSTRACT: Abstract Objective: To first describe laparoscopic nephrectomy (LN) for patients with acute blunt Grade 4 renal injuries using a retroperitoneal approach. Patients and Methods: Three patients (2 males and 1 female) with acute blunt renal trauma underwent retroperitoneal LN successfully. The revised American Association for Surgery of Trauma grading system was used to grade renal injuries. All three patients with Grade 4 renal injuries required blood transfusions preoperatively and angiographic embolization because of hemodynamic instability. Given the severity of the renal injuries, failure of angiographic embolization, and persistent blood loss, surgical intervention was used. We performed retroperitoneal LN using four trocars within 24 hours after trauma for the patients. Results: Pure retroperitoneal LN was successfully performed in all 3 patients without requiring hand-assisted or open surgery. The renal hematoma dimension for the patients was 7.5, 8.4, and 9.2 cm, respectively. Operative time was 80, 110, and 130 minutes, respectively. Estimated blood loss was 100, 140, and 300 mL, respectively. The incision size was 4.2, 4.2, and 4.5 cm, respectively. The average hospital stay was 6 days. Pathology showed renal injuries without incidental renal tumors. Conclusions: Despite the technical challenges, LN for patients with acute blunt Grade 4 renal injuries using a retroperitoneal approach is safe and feasible in carefully selected patients if conservative measures and angiographic embolization fail. However, it is important to note that one should keep a low threshold for open conversion or the hand-assisted approach whenever necessary.
    Journal of Laparoendoscopic & Advanced Surgical Techniques 05/2014; · 1.07 Impact Factor
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    ABSTRACT: Objective To evaluate the production and utilization of Cochrane systematic reviews(CSRs) and to analyze its influential factors, so as to improve the capacity of translating CSRs into practice.Methods All CSRs and protocols were retrieved from the Cochrane Library ISSUE 2, 2011 and citation data were retrieved from SCI database. Citation analysis was used to analyze the situation of CSRs production and utilization.ResultsCSR publication had grown from an annual average of 32 to 718 documents. Only one developing country was among the ten countries with the largest amount of publications. High income countries accounted for 83% of CSR publications and 90.8% of cited counts. 34.7% of CSRs had a cited count of 0, while only 0.9% had been cited more than 50 times. Highly cited CSRs were published in England, Australia, Canada, USA and other high income countries. The countries with a Cochrane center or a Cochrane methodology group had a greater capability of CSRs production and citing than others. The CSRs addressing the topics of diseases were more than those targeted at public health issues. There was a big gap in citations of different interventions even for the same topic.Conclusion The capability of CSR production and translation grew rapidly, but varied among countries and institutions, which was affected by several factors such as the capability of research, the resourcesand the applicability of the evidence. It is important to improve evidence translation through educating, training and prioritizing the problems based on real demands of end user.This article is protected by copyright. All rights reserved.
    Journal of Evidence-Based Medicine 05/2014;
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    ABSTRACT: Recent genome-wide association studies have identified 14 genetic variants associated with bladder cancer in Caucasians. The effects of these risk variants and their cumulative effects in Asian populations are unknown. We genotyped these newly identified variants in a case-control study of 1,050 patients diagnosed with bladder cancer and 1,404 controls in the Chinese population. Odds rations (ORs) and 95% confidence intervals (CIs) were computed by logistic regression, and cumulative effect of risk alleles were evaluated. Overall, seven of the 14 variants were significantly associated with bladder cancer risk (P = 9.763 × 10-3 for rs9642880 at 8q24.21, P = 3.004 × 10-3 for rs2294008 at 8q24.3, P = 0.012 for rs798766 at 4p16.3, P = 0.034 for rs1495741 at 8p22, P = 2.306 × 10-4 for GSTM1, P = 8.507 × 10-8 for rs17674580 at 18q12.3, P = 7.179 × 10-4 for rs10936599 at 3q26.2), and the Odds Ratios (ORs) ranged from 1.13 to 1.65. Moreover, there were a significant increased risk for bladder cancer positively correlated numbers of risk alleles and smoking status (Ptrend = 7.060 × 10-16). However, no allelic interaction effects on bladder cancer risk were observed between cumulative effects of variants and clinical characteristics. These findings suggest that seven bladder cancer risk-associated variants (rs9642880, rs2294008, rs798766, rs1495741, GSTM1 null, rs17674580, and rs10936599) may be used, collectively, to effectively measure inherited risk for bladder cancer. © 2014 Wiley Periodicals, Inc.
    International Journal of Cancer 04/2014; · 6.20 Impact Factor
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    ABSTRACT: Lithocarpus polystachyus leaves have been used as tea beverage and folk medicine for healthy care in the Southwest of China. The purpose of this study is to investigate the anticancer activity of Lithocarpus polystachyus Rehd leaf aqueous extract (LPAE) and to explore the possible mechanism of its activity. Growth inhibition effects of LPAE breast cancer were tested in vitro and in vivo. The possible mechanism of its activity was analyzed with cell biological and molecular biological assays. After LPAE treatment, the proliferation and colony formation of cancer cells decreased; apoptotic cells increased; DNA fragmentations were evident; mRNA and protein expressions of PPARγ, Bax, and caspase-3 genes increased and expressions of cyclin D1 and Bcl-2 genes decreased; in vivo experiment, LPAE inhibited human beast cancer growth. The findings in this experimental study suggested that LPAE has potential cytotoxic and apoptotic effects on human breast cancer cells in vitro and inhibits the cancer growth in vivo, and its mechanism of activity might be associated with apoptosis induction of cancer cells through upregulation of the mRNA and protein expressions of PPARγ, Bax, and capase-3 genes and downregulation of the expressions of cyclin D1 and Bcl-2 genes.
    Nutrition and Cancer 03/2014; · 2.70 Impact Factor
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    ABSTRACT: Cancer stem cells (CSCs) are proposed to be responsible for tumor recurrence, metastasis and the high mortality rate of cancer patients. Isolation and identification of CSCs is crucial for basic and preclinical studies. However, as there are currently no universal markers for the isolation and identification of CSCs in any type of cancer, the method for isolating CSCs from primary cancer tissues or cell lines is costly and ineffective. In order to establish a reliable model of cervical cancer stem cells for basic and preclinical studies, the present study was designed to enrich cervical cancer CSCs using a nonadhesive culture system and to characterize their partial stemness phenotypes. Human cervical cancer cells (HeLa) were cultured using a nonadhesive culture system to generate tumor spheres. Their stemness characteristics were investigated through colony formation, tumor sphere formation, self‑renewal, toluidine blue staining, chemoresistance, invasion assays, reverse transcription‑polymerase chain reaction, immunofluorescence staining of putative stem cell markers, including octamer‑binding transcription factor 4, SRY‑box 2 and aldehyde dehydrogenase 1 family, member A1, and adipogenic differentiation induction. Typical tumor spheres were formed within 5‑7 days under this nonadhesive culture system. Compared with the adherent parental HeLa cells, the colony formation capacity, self‑renewal potential, light cell population, cell invasion, chemoresistance and expression of putative stem cell markers of the tumor sphere cells increased significantly, and a subpopulation of tumor sphere cells were induced into adipogenic differentiation. Using the nonadhesive culture system, a reliable model of cervical cancer stem cells was established, which is inexpensive, effective and simple compared with the ultra‑low attachment serum free culture method. The stemness characteristics of the tumor sphere HeLa cells mirrored the CSC phenotypes. This CSC model may be useful for basic and preclinical studies of cervical cancer and other types of cancer.
    Molecular Medicine Reports 03/2014; · 1.17 Impact Factor
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    ABSTRACT: OBJECTIVES To describe our pure retroperitoneal laparoscopic radical nephrectomy (LRN) with thrombectomy for right renal masses with renal vein (RV) and inferior vena cava (IVC) thrombus. METHODS Five right renal masses with RV and IVC thrombus underwent pure retroperitoneal LRN. Three patients had a history of abdominal surgery. In one patient with a RV thrombus, the RV was ligated and dissected with Hem-o-lok clips; in 4 patients with IVC thrombus, the IVC was partially occluded with a laparoscopic vascular clamp and incised distal to its junction with the right RV, and the thrombus was delivered intact. The IVC was closed with a running 3-zero polypropylene suture. RESULTS Pure retroperitoneal LRN with thrombectomy was successfully performed for all the patients without hand-assisted or open conversion. The mean tumor size was 6.2cm and mean thrombus length was 2.8cm; Four thrombus extended 0.6-1.0cm into the IVC and the mean operative time was 127 minutes with the average estimated blood loss at 148 ml. The mean hospital stay was 5 days. Histology revealed 2 renal cell carcinomas (RCC), 1 angiomylipoma (AML), 1 renal pelvic transitional cell carcinoma (TCC) and 1 renal infarction. All the surgical margins were negative. With a mean follow-up of 35 months, no patients developed metastatic diseases. CONCLUSIONS Despite the technical challenges, pure retroperitoneal LRN for right renal masses with a RV and IVC thrombus is safe and feasible in appropriately selected patients using a retroperitoneal approach. In patients with minimal caval involvement, our surgical approach provided an alternative treatment option, especially when the patients had a history of abdominal surgery.
    Journal of endourology / Endourological Society 03/2014; · 1.75 Impact Factor
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    ABSTRACT: Employee turnover is costly in terms of direct costs and indirect costs faced by organizations. Thus, researchers have investigated turnover for more than two decades. Different methods have been proposed to identify employee turnover risk. However, reducing and preventing employee turnover is still one of the biggest challenges to organizations. Quality function deployment (QFD) method is a systems approach to quality engineering. Employee turnover risk identification mainly involves employee turnover risk factors modelling, identifying risk factors' index, and determining index weights. In this paper, we propose a multi-level employee turnover risk identification model using modified QFD method. This study contributes to the QFD and employee turnover research literature and provides practical guidance to business practitioners. Copyright © 2014 John Wiley & Sons, Ltd.
    Behavioral Science 03/2014;
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    ABSTRACT: Consumer product safety closely relates to consumer health. In this paper, a knowledge engineering framework is proposed for data mining to identify key safety factors from a large number of consumer product safety cases. Data mining in the framework is performed in three steps. The first step is to collect consumer product safety cases, a case can be semistructured or unstructured, and cases can be collected either manually or automatically by a web spider crawling certain websites. The second step is to extract all safety factors from a number of consumer product safety cases. A new method based on linear chain conditional random field is developed to extract safety factors. The effectiveness of the method has been validated on product cases. The third step is to identify a set of key factors from all safety factors by knowledge reasoning. To illustrate the process of knowledge reasoning, a set of 3192 safety cases of electric products with electric shock accidents is chosen as the case study; a Bayesian network based model is developed to retrieve key safety factors relating to electric shock accidents. The performance of the reasoning model has been verified by a combination of experts' evaluation and experiments, and it has shown the proposed reasoning model can help identify key safety factors of electric shock accidents successfully. Overall, the proposed framework is capable of identifying key safety factors from a large number of consumer product safety cases. Copyright © 2014 John Wiley & Sons, Ltd.
    Behavioral Science 03/2014;
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    ABSTRACT: Finding a way to improve students' learning performance is a crucial concern in education reform. Many researchers have investigated possible predictors of student learning performance, including personality traits, learning styles, self-efficacy, and so on. Nevertheless, there is limited research that examines the effectiveness of the interaction between students and teachers, achievement sharing, and the classroom environment. This study investigates the impact of effective communication between students and teachers, achievement sharing, and a positive classroom environment on learning performance through an empirical analysis. We found that when teachers create a sense of community, respond to students and foster positive relationships, students are more engaged and enthusiastic about learning and tend to perform better academically. Copyright © 2014 John Wiley & Sons, Ltd.
    Behavioral Science 03/2014;
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    ABSTRACT: Objective: Efforts have been made by tissue engineers to create a permissive environment for neural regeneration, and to enhance the efficiency of neural stem cell (NSC) transplantation. However, to acquire sufficient number of seed cells on the material appears to be the main obstacle to constructing functional transplantable NSC-biomaterial complexes. A culture system has been optimized in the current study to maintain the specific characteristics of NSCs/neural progenitor cells (NPCs) on the material and achieve sustaining increased multipotent seed cells. Methods: The PHBHHx film was selected as biomaterial and the surface was firstly modified with NaOH treatment. The NSCs/NPCs isolated from the cerebral cortex of rat embryos were cultured on the treated PHBHHx films in growth medium containing 1%, 5%, and 10% fetal bovine serum (FBS). Then the attachment, survival, proliferation, and differentiation of NSCs/NPCs were assessed. Results: NaOH treatment significantly increased the hydrophilicity of PHBHHx and enhanced NSCs/NPCs attachment. On the treated PHBHHx film, NSCs/NPCs survived well and actively proliferated in the medium containing 1% FBS. After 7-14 days in culture, approximately two-thirds of cells remained as nestin and Sox2 positive NSCs/NPCs. However, in the medium containing 5% and 10% FBS, NSCs/NPCs proliferation was reduced and differentiation, particularly glial differentiation was significantly promoted. Conclusion: Growth medium containing low concentration of FBS is favorable for maintaining the characteristics, in terms of self-renewal and multiple differentiation, of NSCs/NPCs on NaOH-treated PHBHHx films. This could be a useful method to construct functional transplantable NSCs/NPCs-biomaterial complex.
    Neurological Research 03/2014; 36(3):207-14. · 1.18 Impact Factor
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    ABSTRACT: A sensitive and selective electrochemical method was developed for the detection of DNA methylation, determination of DNA methyltransferase (MTase) activity and screening of MTase inhibitor. Methylene blue (MB) was employed as electrochemical indicator and DNA-modified gold nanoparticles (AuNPs) were used as signal amplification unit because the DNA strands in this composite have strong adsorption ability for MB. First, the thiolated single-stranded DNA S1 was self-assembled on gold electrode, hybridization between the lower portion of DNA S1 and its complementary DNA S2 formed an identical double-stranded tetranucleotide target sequence for both DNA adenine methylation (Dam) MTase and methylation-resistant endonuclease Mbo I, then the upper portion of DNA S1 was hybridized with its complementary DNA S3 modified on AuNPs to bring the DNA S3-AuNPs amplification units onto the electrode. The DNA S1/S2/S3-AuNPs bioconjugate has lots of DNA strands, and they can adsorb abundant MB. Mbo I endounuclease could not cleave the identical target sequence after it was methylated by Dam MTase. On the contrary, the sequence without methylation could be cleaved, which would decrease the amount of adsorbed MB. The presence of redox-active MB was detected electrochemically by differential pulse voltammetry (DPV). Thus, the activity of Dam MTase and methylation status were sensitively converted to the DNA S3-AuNPs amplified DPV signals. The DPV signal demonstrated a linear relationship with logarithm of Dam concentration ranging from 0.075 to 30U/mL, achieving a detection limit of 0.02U/mL (S/N=3). Also, screening of Dam MTase inhibitor 5-fluorouracil was successfully investigated using this fabricated sensor.
    Biosensors & bioelectronics 02/2014; 58C:40-47. · 5.43 Impact Factor
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    ABSTRACT: The role of [-2]proPSA (p2PSA) based diagnostic tests for the detection of aggressive prostate cancer (PCa) has not been fully evaluated. We conducted a meta-analysis to evaluate the diagnostic performance of p2PSA/free PSA (%p2PSA) and prostate health index (Phi) tests for PCa and to evaluate their ability in discriminating between aggressive and non-aggressive PCa. A total of 16 articles were included in this meta-analysis. For the detection of PCa, the pooled sensitivity, specificity, and AUC were 0.86 (95% CI, 0.84-0.87), 0.40 (95% CI, 0.39-042) and 0.72 (95% CI, 0.67-0.77) for %p2PSA respectively, and were 0.85 (95% CI, 0.83-0.86), 0.45 (95% CI, 0.44-0.47) and 0.70 (95% CI = 0.65-0.74) for Phi, respectively. In addition, the sensitivity for discriminating PCa between higher Gleason score (≥7) and lower Gleason score (<7) was 0.96 (95% CI, 0.93-0.98) and 0.90 (95% CI, 0.87-0.92) for %p2PSA and Phi respectively, and the specificity was low, only 0.09 (95% CI, 0.06-0.12) and 0.17 (95% CI, 0.14-0.19) for %p2PSA and Phi, respectively. Phi and %p2PSA have a high diagnostic accuracy rates and can be used in PCa diagnosis. Phi and %p2PSA may be useful as tumor markers in predicating patients harboring more aggressive disease and guiding biopsy decisions.
    Scientific reports. 01/2014; 4:5012.
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    ABSTRACT: Carbonic anhydrase IX (CA IX), a hypoxia-inducible protein in tumors, has been shown to be valuable for the prognosis of nasopharyngeal carcinoma (NPC). However, the function and mechanism of CA IX has been not explored in NPC. Here, we found that CA IX was detected at higher levels in NPC cells and tissues than their corresponding partners. Furthermore, the cell growth, migration and invasion in vitro were altered with shRNA or overexpression of CA IX in NPC cells. More importantly, the metastatic ability of NPC cells stably expressing CA IX was significantly enhanced using the hepatic metastasis model of nude mice in vivo. Finally, the mTOR pathway was indicated to be involved in such effects of CA IX on NPC. This is the first evidence that CA IX may promote the NPC metastasis to potentially be a therapeutic target for NPC, and that the inhibitory molecules of CA IX and/or the mTOR pathway alone or combination with both may be worth to have a clinical trial for the patients with NPC.
    International journal of clinical and experimental pathology. 01/2014; 7(6):2942-9.
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    ABSTRACT: Automated information retrieval is critical for enterprise information systems to acquire knowledge from the vast amount of data sets. One challenge in information retrieval is text classification. Current practices rely heavily on the classical naïve Bayes algorithm due to its simplicity and robustness. However, results from this algorithm are not always satisfactory. In this article, the limitations of the naïve Bayes algorithm are discussed, and it is found that the assumption on the independence of terms is the main reason for an unsatisfactory classification in many real-world applications. To overcome the limitations, the dependent factors are considered by integrating a term frequency–inverse document frequency TF-IDF weighting algorithm in the naïve Bayes classification. Moreover, the TF-IDF algorithm itself is improved so that both frequencies and distribution information are taken into consideration. To illustrate the effectiveness of the proposed method, two simulation experiments were conducted, and the comparisons with other classification methods have shown that the proposed method has outperformed other existing algorithms in terms of precision and index recall rate.
    Enterprise Information Systems 01/2014; 8(1):107-120. · 9.26 Impact Factor
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    ABSTRACT: A novel electrochemical aptasensor was developed for ultrasensitive detecting staphylococcal enterotoxin B (SEB) by combining signal amplification and target-induced aptamer release strategy. Target SEB could efficiently induce the release of aptamers from the eletrode surface, thus leading to substantial enzyme-amplified electrochemical signal in the presence of H2O2.
    Colloids and surfaces B: Biointerfaces 01/2014; · 4.28 Impact Factor
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    ABSTRACT: The growth regulator mepiquat chloride (MC) is globally used in cotton (Gossypium hirsutum L.) canopy manipulation to avoid excess growth and yield loss. However, little information is available as to whether the modification of plant architecture by MC is related to alterations in gibberellic acid (GA) metabolism and signaling. Here, the role of GA metabolism and signaling was investigated in cotton seedlings treated with MC. The MC significantly decreased endogenous GA3 and GA4 levels in the elongating internode, which inhibited cell elongation by downregulating GhEXP and GhXTH2, and then reducing plant height. Biosynthetic and metabolic genes of GA were markedly suppressed within 2 to 10 d of MC treatment, which also downregulated the expression of DELLA-like genes. A remarkable feedback regulation was observed at the early stage of MC treatment when GA biosynthetic and metabolic genes expression was evidently upregulated. Mepiquat chloride action was controlled by temporal translocation and spatial accumulation which regulated GA biosynthesis and signal expression for maintaining GA homeostasis. The results suggested that MC application could reduce endogenous GA levels in cotton through controlled GA biosynthetic and metabolic genes expression, which might inhibit cell elongation, thereby shortening the internode and reducing plant height.
    Plant Science. 01/2014;
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    ABSTRACT: Microspheres assembled using cryptomelane-type KMn8O16 nanorods are synthesized via a facile template-free, single-step hydrothermal technique. The synthesized KMn8O16 generates nanorods 10-20 nm in diameter and approximately 300-1000 nm long. The rods self-assemble to form microspheres of 2-6 μm in diameters. The electron transfer number for KMn8O16 during the ORR is approximately 3.98 at 0.5 V vs. Hg/HgO, and the H2O2 percentage is 0.66%. Moreover, a direct methanol fuel cell (DMFC) is built using KMn8O16 as cathodic catalyst, PtRu/C alloy as the anodic catalyst and a polymer fiber membrane (PFM) instead of a conventional polymer electrolyte membrane (PEM). The peak power densities (43.3 mW cm-2 and 153.9 mW cm-2) have been achieved at 25 °C and 70 °C, respectively. KMn8O16 shows good electrocatalytic activity and stability during oxygen reduction in alkaline solutions and demonstrates tolerance toward methanol poisoning.
    Journal of Power Sources 01/2014; 267:33–38. · 5.26 Impact Factor
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    ABSTRACT: An ultrasensitive and specific electrochemical biosensor for the determination of mecA gene, which is associated with methicillin resistant strains, was successfully developed. Capture probes were immobilized on the surface of a gold nanoparticle modified glassy carbon electrode. After target probes were hybridized with capture probes, dual labeled gold nanoparticles with alkaline phosphatase and detection probes were hybridized with target probes for sensitive detection of mecA gene fragments. Dual labeled gold nanoparticles, which acted as a high-efficiency signal amplification strategy, were successfully prepared. The gold nanoparticles and dual labeled gold nanoparticles were characterized by transmission electron microscopy. Different concentrations of gene fragments were detected to inspect the specificity and sensitivity of the proposed biosensor. This biosensor displayed outstanding selectivity and a remarkable sensitivity (LOD = 23 pM, S/N = 3). Good linearity over the range of 50 pM to 250 pM was demonstrated. Moreover, the measurement process required less than 2 hours. This device was employed for the successful determination of mecA gene from methicillin resistant Staphylococcus aureus.
    Analytical Letters 01/2014; 47(4). · 0.97 Impact Factor

Publication Stats

622 Citations
406.77 Total Impact Points


  • 2014
    • Sun Yat-Sen University Cancer Center
      Shengcheng, Guangdong, China
    • China Agricultural University
      • College of Agronomy and Biotechnology
      Peping, Beijing, China
    • Tianjin Medical University Cancer Institute and Hospital
      T’ien-ching-shih, Tianjin Shi, China
    • Beijing Jiaotong University
      • School of Traffic and Transportation
      Peping, Beijing, China
    • Xi'an Jiaotong University
      • State Key Laboratory for Mechanical Behavior of Materials
      Ch’ang-an, Shaanxi, China
  • 2013–2014
    • Wake Forest School of Medicine
      • Center for Cancer Genomics
      Winston-Salem, North Carolina, United States
    • Wake Forest University
      Winston-Salem, North Carolina, United States
    • Fourth Military Medical University
      Xi’an, Liaoning, China
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China
    • Changhai Hospital, Shanghai
      Shanghai, Shanghai Shi, China
  • 2012–2014
    • Chongqing Medical University
      Ch’ung-ch’ing-shih, Chongqing Shi, China
    • Beijing Medical University
      • Department of Pathophysiology
      Peping, Beijing, China
  • 2010–2014
    • Beijing University of Aeronautics and Astronautics (Beihang University)
      • School of Economics and Management
      Peping, Beijing, China
  • 2008–2014
    • Sichuan University
      • • Analytical Center
      • • Department of Chemistry
      Hua-yang, Sichuan, China
  • 2011–2012
    • Chengdu University Of Traditional Chinese Medicine
      Hua-yang, Sichuan, China
    • McMaster University
      Hamilton, Ontario, Canada
  • 2007–2011
    • West China School of Medicine
      Hua-yang, Sichuan, China
    • Shanghai Jiao Tong University
      • Department of Cardiology (Renji)
      Shanghai, Shanghai Shi, China
  • 2009
    • University of Queensland 
      • School of Biomedical Sciences
      Brisbane, Queensland, Australia
    • Prince Henry's Institute
      Melbourne, Victoria, Australia
    • Northeast Institute of Geography and Agroecology
      • Shanghai Institutes for Biological Sciences
      Peping, Beijing, China
    • Agricultural University Of Hebei
      Pao-ting-shih, Hebei, China
    • Shanghai Institute of Measurement and Testing Technology
      Shanghai, Shanghai Shi, China
    • China Pharmaceutical University
      • Department of Pharmaceutical Analysis
      Nanjing, Jiangxi Sheng, China
  • 2004–2009
    • Huazhong Agricultural University
      • College of Life Science and Technology
      Wuhan, Hubei, China