Lin Zhang

Xi'an Jiaotong University, Xi’an, Shaanxi Sheng, China

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Publications (3)7 Total impact

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    ABSTRACT: This paper quantificationally probes into time-varying migration processes of moving neutralization boundary (MNB) on immobilized pH gradient (IPG) strip in ammonia-rehydration buffers. The time-varying migration processes are determined by both time-varying dissociation equilibria of ammonia and position-varying pH environments formed by immobilized carrier ampholytes (CAs) on the IPG strip. Thus, the local dissociation equilibria of ammonia and the position-varying pH are introduced into the recursion equation of position of MNB migrations. The theoretical position-time curves and the velocity-time curves of MNB migrations obtained by the recursion approach were satisfactorily validated by a series of images of boundary migrations from the IPG-MNB experiments by using rehydration buffers with different ammonia concentrations on pH 3-6 IPG strips. The results achieved herein have significant evidence to a quantificational understanding of the mechanism of MNB and IEF.
    Journal of Separation Science 05/2011; 34(10):1212-9. · 2.59 Impact Factor
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    ABSTRACT: Moving neutralization boundary (MNB) is an important foundation to understand and improve IEF. However, there are obstacles in theoretical predictions of MNB on IPG strips due to the unknown local concentrations of carrier ampholytes on commercial IPG strips and the time-varying boundary velocities. We introduce a recursion approach to extend the current MNB theories into the space-time varying MNB system. The recursion approach emphasizes the localizability of physicochemical parameters in the discrete time intervals and local positions in Lagrangian coordinates, such as local concentrations of carrier ampholytes, local OH concentrations, local boundary velocities, local judgments, etc. The boundary-position recursion equation in a complete time sequence was presented to quantitatively predict the MNB position-time curves by distinguishing three kinds of titration cases according to NaOH concentrations in rehydration buffers. The theoretical position-time curves and local relative judgments of boundaries were satisfactorily validated by corresponding images of boundary migrations achieved from the IPG-MNB experiments with the some typical NaOH concentrations-bromophenol blue-rehydration buffers on pH 4-7 IPG strips. The results achieved herein have evident significances to the development of moving reaction boundary and IEF.
    Electrophoresis 09/2009; 30(18):3134-43. · 3.26 Impact Factor
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    ABSTRACT: To better understand the natural killer (NK) cell cytotoxicity mechanism at the proteome level, we comparatively analyzed the proteome of the human NK-92 cells which participate in NK cell-mediated cytotoxicity assay and that of control cells. Soluble proteins were separated by two-dimensional gel electrophoresis (2-DE), 75 protein spots were found to be reproducibly differentially expressed between control and cytotoxic human NK-92 cells. A total of 60 different proteins were unequivocally identified by MALDI-TOF MS coupled with database interrogation; 37 proteins were up-regulated, whereas 23 proteins were down-regulated. Western blotting analysis of heat shock protein 60 (HSP60) and cathepsin W verified their proteome results. Some of identified proteins are involved in NK-92 cytotoxicity, which is consistent with the literature. In addition, we modeled the pathway networks between differentially expressed proteins and cellular processes of secretion and exocytosis through PathwayStudio software. The results of this study help to provide insight into the molecular mechanism of NK cell cytotoxicity.
    Biochemistry (Moscow) 08/2007; 72(7):716-27. · 1.15 Impact Factor

Publication Stats

9 Citations
7.00 Total Impact Points

Institutions

  • 2009
    • Xi'an Jiaotong University
      • Key Laboratory of Biomedical Information Engineering of Ministry of Education
      Xi’an, Shaanxi Sheng, China