Lin Li

Publications (15)57.47 Total impact

• Article: Recognize three different human telomeric G-quadruplex conformations by quinacrine.

  Hongxia Sun, Junfeng Xiang, Qian Li, Yan Liu, Lin Li, Qian Shang, Guangzhi Xu, Yalin Tang
  [show abstract] [hide abstract]
  ABSTRACT: Recognition of different human telomeric G-quadruplex structures has been a very important task for developing anti-cancer drug design. However, it also is a very challenging question since multiple conformational isomers of telomeric G-quadruplexes coexist under some conditions. Here, three different conformations including parallel, antiparallel, and mixed-type telomeric G-quadruplex structures have been well recognized by quinacrine (QNA) through monitoring its absorption, fluorescence, and fluorescence lifetime spectra. The multiple structures of H22 G-quadruplexes under physiological K(+) conditions could also be easily determined to coexist as mixed-type and antiparallel G-quadruplexes. The recognition mechanism based on the different binding affinity and binding sites has been further elucidated by association with the nuclear magnetic resonance (NMR) results.
  The Analyst 02/2012; 137(4):862-7. · 4.23 Impact Factor
• Article: Screen anti-influenza lead compounds that target the PA(C) subunit of H5N1 viral RNA polymerase.

  Lin Li, Shenghai Chang, Junfeng Xiang, Qian Li, Huanhuan Liang, Yalin Tang, Yingfang Liu
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  ABSTRACT: The avian influenza (H5N1) viral RNA polymerase protein PA(C) was used as a target to screen nine chlorogenic acid derivatives for their polymerase inhibitor activity. Among them, seven compounds were PA(C) ligands, and four inhibited influenza RNA polymerase activity. These results aid in the design of anti-influenza agents based on caffeoylquinic acid.
  PLoS ONE 01/2012; 7(8):e35234. · 4.09 Impact Factor
• Article: Correction to "Effects of Loops and Nucleotides in G-Quadruplexes on Their Interaction with an Azacalixarene, Methylazacalix[6]pyridine"

  Ai-Jiao Guan, En-Xuan Zhang, Jun-Feng Xiang, Qian Li, Qian-Fan Yang, Lin Li, Ya-Lin Tang, Mei-Xiang Wang
  The Journal of Physical Chemistry B 12/2011; · 3.70 Impact Factor
• Source

  Available from: InTech

  Chapter: Metabolomics and Transcriptional Responses in Estrogen Receptor Positive Breast Cancer Cells

  Soma Mandal, Protiti Khan, Lin Li, James R. Davie
  11/2011; , ISBN: 978-953-307-714-7
• Article: Effects of loops and nucleotides in G-quadruplexes on their interaction with an azacalixarene, methylazacalix[6]pyridine.

  Ai-Jiao Guan, En-Xuan Zhang, Jun-Feng Xiang, Qian Li, Qian-Fan Yang, Lin Li, Ya-Lin Tang, Mei-Xiang Wang
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  ABSTRACT: A novel trend in G-quadruplex ligand design is to build a binder that is able to not only discriminate G-quadruplex from duplex-DNA, but also among various G-quadruplex structures. Methylazacalix[6]pyridine (MACP6), a new type of azacalixarene with flexible conformation, exhibits induced circular dichroism signals when interacted with most of G-quadruplexes. The intensities of the induced signals are strongly dependent on the topology of G-quadruplexes. Further evidence has shown that these signals can be ascribed to the preferred binding of MACP6 to the loops of G-quadruplexes, which rely on the nature of nucleotides in the loops.
  The Journal of Physical Chemistry B 09/2011; 115(43):12584-90. · 3.70 Impact Factor
• Article: Fishing potential antitumor agents from natural plant extracts pool by dialysis and G-quadruplex recognition.

  Qian Shang, Jun-Feng Xiang, Xiu-Feng Zhang, Hong-Xia Sun, Lin Li, Ya-Lin Tang
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  ABSTRACT: Screening G-quadruplex ligands from natural plants is important because the ligands may be potential antitumor drugs. A new screening strategy is proposed based on the combination of dialysis and G-quadruplex recognition technique which could separates G-quadruplex ligand from natural extracts and elucidate the structure of this ligand. This result offers a novel approach to obtain active antitumor compounds.
  Talanta 07/2011; 85(1):820-3. · 3.79 Impact Factor
• Article: A stabilizing and denaturing dual-effect for natural polyamines interacting with G-quadruplexes depending on concentration.

  Hongxia Sun, Junfeng Xiang, Yan Liu, Lin Li, Qian Li, Guangzhi Xu, Yalin Tang
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  ABSTRACT: Both G-quadruplexes and natural polyamines are intimately associated with tumor growth and proliferation. The effect of the natural polyamines on telomeric and some oncogenic G-quadruplexes including bcl-2, c-kit, and c-myc G-quadruplexes has been studied by using absorption, fluorescence, CD, and NMR methods. The results exhibited an interesting dual-effect depending on polyamineś concentration. Polyamines promote and stabilize G-quadruplexes under a lower concentration (less than 1mM) but denature G-quadruplexes under a higher concentration (more than 1mM). Probably the electrostatic and hydrophobic effect of polyamines and the hydrogen-bonding interaction between guanines and polyamines were respectively responsible for the stabilizing and denaturing effect.
  Biochimie 06/2011; 93(8):1351-6. · 3.02 Impact Factor
• Article: Verification of intramolecular hybrid/parallel g-quadruplex structure under physiological conditions using novel cyanine dye H-aggregates: both in solution and on Au film.

  Qianfan Yang, Jun-Feng Xiang, Shu Yang, Qian Li, Qiuju Zhou, Aijiao Guan, Lin Li, Yanxia Zhang, Xiufeng Zhang, Hong Zhang, Yalin Tang, Guangzhi Xu
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  ABSTRACT: The H-aggregates of a novel cyanine dye, 3,3'-di(3-sulfopropyl)-4,5,4',5'-dibenzo-9-methyl-thiacarbocyanine triethylammonium salt (MTC), have been fabricated to verify hybrid/parallel intramolecular G-quadruplexes from linear duplex and single-strand DNAs under physiological conditions. The recognition is found to be successful both in solution and on Au film. These results have shown MTC H-aggregates, as a supramolecular system, may be used as a potential excellent probe for DNA structure, both in vitro and in vivo.
  Analytical Chemistry 10/2010; 82(22):9135-7. · 5.86 Impact Factor
• Article: Stabilizing parallel G-quadruplex DNA by a new class of ligands: two non-planar alkaloids through interaction in lateral grooves.

  Qian Li, Junfeng Xiang, Xudong Li, Lirong Chen, Xiaojie Xu, Yalin Tang, Qiuju Zhou, Lin Li, Hong Zhang, Hongxia Sun, Aijiao Guan, Qianfan Yang, Shu Yang, Guangzhi Xu
  [show abstract] [hide abstract]
  ABSTRACT: Human DNA sequences consisting of tandem guanine (G) nucleotides can fold into a four-stranded structure named G-quadruplex via Hoogsteen hydrogen bonding. As the sequences forming G-quadruplex exist in essential regions of eukaryotic chromosomes and are involved in many important biological processes, the study of their biological functions has currently become a hotspot. Compounds selectively binding and stabilizing G-quadruplex structures have the potential to inhibit telomerase activity or alter oncogene expression levels and thus may act as antitumor agents. Most of reported G-quadruplex ligands generally have planar structures which stabilize G-quadruplex by pi-pi stacking. However, based on a pharmacophore-based virtual screening two non-planar G-quadruplex ligands were found. These two ligands exhibit good capability for G-quadruplex stabilization and prefer binding to paralleled G-quadruplex rather than to duplex DNA. The binding of these ligands to G-quadruplex may result from groove binding at a 2:1 stoichiometry. These results have shown that planar structures are not essential for G-quadruplex stabilizers, which may represent a new class of G-quadruplex-targeted agents as potential antitumor drugs.
  Biochimie 04/2009; 91(7):811-9. · 3.02 Impact Factor
• Article: Fast screening and structural elucidation of G-quadruplex ligands from a mixture via G-quadruplex recognition and NMR methods.

  Qiuju Zhou, Lin Li, Junfeng Xiang, Hongxia Sun, Yalin Tang
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  ABSTRACT: Currently, there is a considerable interest in discovering G-quadruplex ligands. Plant-derived agents, because of their diversity in structure and bioactivity and low toxicity, may be a very diverse source of G-quadruplex ligands. However, up to now, the screening of G-quadruplex ligands from natural plant extract has not been reported. Herein, in order to develop a simple method for fast identifying G-quadruplex ligands from plant extract, we intended to substitute the spectral shift in the imino region (delta 10-12) in (1)H NMR spectra of G-quadruplex for in vitro bioassay to judge the existence/nonexistence of G-quadruplex ligand(s) in plant extract, and then couple G-quadruplex recognition with NMR based structure elucidation to identify the structure of the ligand(s) without the need of prior separation. In this paper, we successfully screened a G-quadruplex ligand from a simulated plant extract using this approach. This research work provides a promising tactic to find new leading compounds from nature plant extract.
  Biochimie 11/2008; 91(2):304-8. · 3.02 Impact Factor
• Article: Screening Potential Antitumor Agents from Natural Plant Extracts by G‐Quadruplex Recognition and NMR Methods

  Qiuju Zhou, Lin Li, Junfeng Xiang Dr, Yalin Tang Prof, Hong Zhang, Shu Yang, Qian Li, Qianfan Yang, Guangzhi Xu Prof
  Angewandte Chemie 06/2008; 120(30):5672 - 5674.
• Article: Screening potential antitumor agents from natural plant extracts by G-quadruplex recognition and NMR methods.

  Qiuju Zhou, Lin Li, Junfeng Xiang, Yalin Tang, Hong Zhang, Shu Yang, Qian Li, Qianfan Yang, Guangzhi Xu
  Angewandte Chemie International Edition 02/2008; 47(30):5590-2. · 13.45 Impact Factor
• Article: Investigation on the interaction between a heterocyclic aminal derivative, SBDC, and human serum albumin.

  Qiuju Zhou, Junfeng Xiang, Yalin Tang, Jiangpeng Liao, Chuyi Yu, Hong Zhang, Lin Li, Yueyang Yang, Guangzhi Xu
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  ABSTRACT: The interaction between a novel promising drug (spiro[(2R,3R,4S)-4-benzyloxy-2,3-isopropylidene-dioxy-1-oxa-cyclopentane-5,5'-(2-benzoylmethylene-1,3-diaza-cyclohexane)] (SBDC)) and human serum albumin (HSA) under physiological conditions has been investigated by using fluorescence, absorption, and circular dichroism (CD) spectroscopic techniques in combination with protein-ligand docking study. It was observed that SBDC has a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The association constants of SBDC with HSA were determined at different temperatures based on fluorescence quenching results. The negative DeltaH and positive DeltaS values in case of SBDC-HSA complex showed that apart from an initial hydrophobic association, both van der Waals interactions and hydrogen bonding play a vital role in the binding of SBDC to HSA. The quantitative analysis data of CD spectra showed that the binding of SBDC to HSA induced conformational changes in HSA and the alpha-helix of 52.1% in free HSA increased to 55.7% in HSA-SBDC complex. The distance between donor (HSA) and acceptor (SBDC) was obtained according to the Förster's theory of non-radiation energy transfer. Data obtained by spectroscopic techniques and protein-ligand docking study suggested that SBDC binds to residues located in subdomain IIA of HSA.
  Colloids and Surfaces B Biointerfaces 02/2008; 61(1):75-80. · 3.46 Impact Factor
• Article: Metabonomics analysis of the urine of rats with Qi deficiency and blood stasis syndrome based on NMR techniques

  Lin Li, JianNong Wang, JianXun Ren, JunFeng Xiang, YaLin Tang, JianXun Liu, Ding Han
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  ABSTRACT: Metabonomics analysis of the urine of rats with Qi deficiency and blood stasis syndrome has been performed by comparison with those of normal rats based on NMR techniques. The relative contents of formate, creatinine, 2-oxoglutarate (2-OG), citrate, taurine, trimethylamine-N-oxide(TMAO), succinate and hippurate in the urine of the rats with Qi deficiency and blood stasis syndrome have been changed. These results have provided evidence for understanding the mechanism and the therapy of Qi deficiency and blood stasis syndrome.
  Chinese Science Bulletin 10/2007; 52(22):3068-3073. · 1.32 Impact Factor
• Article: Protein conformation changes induced by novel organophosphate-containing water-soluble derivative of C60 fullerene nanoparticle

  Xiufeng Zhang, Chunying Shu, Ling Xie, Chunru Wang, Yazhou Zhang, Junfeng Xiang, Lin Li, Yalin Tang
  The Journal of Physical Chemistry C 01/2007; 111:14327-14333. · 4.80 Impact Factor