ABSTRACT: Radioimmunoassay of lipomodulin has been developed using a monoclonal anti-lipomodulin antibody and 125I-labelled lipomodulin. Lipomodulin activity was measured in peritoneal lavage fluids obtained from rats injected with dexamethasone by radioimmunoassay and by enzymatic assay with phospholipase A2. Three species of immunoreactive substances with Mr= 40,000, 30,000 and 16,000 were found. While two species of Mr= 40,000 and 30,000 had phospholipase inhibitory activities, the species of Mr= 16,000 could inhibit phospholipase A2 only after dephosphorylation by alkaline phosphatase treatment.
Biochemical and Biophysical Research Communications 12/1982; 109(1):223-30. · 2.48 Impact Factor