Publications (4)3.09 Total impact
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Conference Proceeding: An Overview of Current Achievements and Shortcomings in Developing Maritime Pine Somatic Embryogenesis and Enabling Technologies in France
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ABSTRACT: Pinus pinaster Ait. is a major conifer in France covering over 1 million ha in intensively managed plantation forests. Together with high productivity (11.8 m3/ha/year), mechanization of marketed harvests (70%) resulted in a significant contribution of this species to the national pulpwood (27.1%, 3.3 million m3) and softwood timber production (24.7%, 5.6 million m3). In France 34 000 workers are employed in this industry (16.5% of the forest sector) with an annual turnover of around 2.5 billion Euros (36% as exports). Breeding programs launched in the early sixties by FCBA and INRA were combined in 1995 into a joined initiative called “Maritime Pine for the Future” that involved all other major forest actors (CPFA, CRPF, ONF). Up to 15% genetic gains were achieved for volume and straightness in first and second generation varieties. Lower genetic gains (10%) are however expected by the third round of selection owing to genetic redundancy within breeding populations. Moreover, maritime pine has a low ability for conventional clonal propagation that would facilitate efficient capture and deployment of the best genetic stocks. The need for an efficient mass propagation system has also dramatically increased because of two recent heavy storms that resulted in a complete clearing of 300 000 ha of forest. To face this challenging task, FCBA and INRA are jointly developing somatic embryogenesis (SE) as a critical enabling technology for efficient elite tree selection, clonal propagation of improved varieties and cryopreservation of both natural and newly obtained genetic resources (Klimaszewska et al. 2007). SE is also providing the plant regeneration system for genetic engineering (Trontin et al. 2007) and serves as a research tool to access the reverse genetics towards marker-aided selection of valuable traits. We will briefly review our major achievements pointing out the current limitations for practical use.Park, Y.S., Bonga, J.M, Park, S.Y., and Moon, H.K. (eds. 2011), IUFRO Working Party 2.09.02: “Somatic Embryogenesis of Trees: conference on ”Advances in Somatic Embryogenesis of Trees and Its Application for the Future Forests and Plantations, pp100-102, Suwon, Republic of Korea, August 19-21; 01/2010 -
Article: Recent Progress in Somatic Embryogenesis of Four Pinus spp.
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ABSTRACT: Advances in conifer biotechnology offer new opportunities in the field of vegetative propagation and genetic engineering. Development of clonal propagation methods, especially somatic embryogenesis (SE), has numerous potential applications. Owing to its efficiency in plant regeneration, coupled with the ease of long-term storage in liquid nitrogen, SE became an indispensable tool for accelerating the development of tree varieties and deployment. Early SE protocols were developed for Picea species, however, when applied to Pinus species, especially at the somatic embryo maturation stage, they were unsuccessful. It became apparent that pines required more research and development to bring the SE biotechnology to its potential. This review emphasizes the most recent progress made in SE and cryopreservation in the genus Pinus, focusing on major plantation and forest species in Europe (Pinus pinaster), New Zealand, Australia, and South America (P. radiata), as well as North America (P. taeda and P. strobus). Much consideration is also given to applying SE in pine improvement and deployment strategies in multivarietal forestry.Tree and Forestry Science and Biotechnology. 01/2007; 1:11-25. -
Article: Recent Progress in Genetic Transformation of Four Pinus spp.
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ABSTRACT: Advances in conifer tissue culture and genetic transformation offer new opportunities in the field of genetic engineering. Genetic transformation is an important tool for breeders because it allows them to introduce valuable genes that might otherwise be difficult to integrate into elite genotypes. Combined with mass vegetative propagation, such as somatic embryogenesis, regeneration of transgenic conifers could result in accelerated tree improvement. Genetic transformation requires the development of an efficient gene delivery system. Extensive work has been done in Pinus spp. to improve transformation methods, either by DNA-coated particle bombardment or by co-culture with Agrobacterium tumefaciens. This review describes the most recent progress made in genetic transformation in the genus Pinus, with emphasis on four important forest and plantation species grown in Europe (P. pinaster), New Zealand, Australia and South America (P. radiata), and North America (P. taeda and P. strobus). The biosafety issues associated with potential deployment of transgenic pine varieties in commercial forestry are highlighted.Transgenic Plant Journal. 01/2007; 1:314-319. -
Article: Initiation of somatic embryogenesis in Pinus banksiana , P. strobus , P . pinaster , and P. sylvestris at three laboratories in Canada and France
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ABSTRACT: During 2002–2004, three laboratories in Canada and France collaborated to improve initiation of somatic embryogenesis (SE) in jack pine (Pinus banksiana Lamb.), Eastern white pine (P. strobus L.), maritime pine (P. pinaster Ait.), and Scots pine (P. sylvestris L.), giving particular attention to the effects of (1) N-(2-chloro-4-pyridyl)-N¢-phenylurea (CPPU) versus various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA), (2) differences in basal nutrient media, i.e., macro- and microelements, and (3) gelling agent concentration. The work was carried out separately at each laboratory, but the details of media compositions were shared and tested on their respective species. Results indicate that the developmental stage of the zygotic embryo (ZE) and genotype effects had a large influence on SE initiation, and that genetic effects were consistent over time. Different species responded differently to PGR types and concentration, basal nutrient media, trace elements, and their combinations. Currently, our best initiation rates based on a selected group of genotypes, optimal development stage of ZE, and medium are 3.9% for jack pine, 54.6% for eastern white pine, 76.2% for maritime pine, and 19.7% for Scots pine.Plant Cell Tissue and Organ Culture 01/2006; 86:87-101. · 3.09 Impact Factor
