Kyung Tae Kim

Chungbuk National University, Chinsen, Chungcheongbuk-do, South Korea

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Publications (148)279.45 Total impact

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    ABSTRACT: Intercellular bridges are a conserved feature of spermatogenesis in mammalian germ cells and derive from arresting cell abscission at the final stage of cytokinesis. However, it remains to be fully understood how germ cell abscission is arrested in the presence of general cytokinesis components. The TEX14 (testis-expressed gene 14) protein is recruited to the midbody and plays a key role in the inactivation of germ cell abscission. To gain insights into the structural organization of TEX14 at the midbody, we have determined the crystal structures of the EABR [endosomal sorting complex required for transport (ESCRT) and ALIX-binding region] of CEP55 bound to the TEX14 peptide (or its chimeric peptides) and performed functional characterization of the CEP55-TEX14 interaction by multiexperiment analyses. We show that TEX14 interacts with CEP55-EABR via its AxGPPx3Y (Ala793, Gly795, Pro796, Pro797, and Tyr801) and PP (Pro803 and Pro804) sequences, which together form the AxGPPx3YxPP motif. TEX14 competitively binds to CEP55-EABR to prevent the recruitment of ALIX, which is a component of the ESCRT machinery with the AxGPPx3Y motif. We also demonstrate that a high affinity and a low dissociation rate of TEX14 to CEP55, and an increase in the local concentration of TEX14, cooperatively prevent ALIX from recruiting ESCRT complexes to the midbody. The action mechanism of TEX14 suggests a scheme of how to inactivate the abscission of abnormal cells, including cancer cells.
    Proceedings of the National Academy of Sciences 09/2015; 112(40):201418606. DOI:10.1073/pnas.1418606112 · 9.67 Impact Factor
  • Kyung Tae Kim · Jae-hyung Kim ·

    Albrecht von Graæes Archiv für Ophthalmologie 08/2015; DOI:10.1007/s00417-015-3139-3 · 1.91 Impact Factor
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    ABSTRACT: An effective and rapid method was developed for the simultaneous identification of aloe, catechu and gambir by high performance liquid chromatography-diode array detector (HPLC-DAD). Identification of three maker compounds presented in three medicinal materials was performed on high performance liquid chromatography-mass spectrometry (HPLC-MS). Under the optimal HPLC chromatographic conditions, sixty-two samples were processed on an Optimapak C18 column using a solvent system of acetonitrile (from 10% to 35%) and 0.1% phosphoric acid solution (from 90% to 65%) at a total flow rate of 1.0mL/min and detected at 270nm. All calibration curves exhibited good linear relationship (r(2)>0.9992). The relative standard deviation values of intra-day and inter-day precision were less than 1% and 2%, respectively. The recoveries of three analytes ranged from 99.48 to 100.97% with low RSDs (<2%). For the first time, this study demonstrates that the processed aloe, catechu and gambir are sold in local material markets in China and Korea without their correct identification. It indicates the existent of high potential medicinal risk by misuse of three medicinal materials. The developed HPLC method can be applied to prevent unexpected biological activity due to misapplication of medicinal materials. Copyright © 2015 Elsevier B.V. All rights reserved.
    Journal of pharmaceutical and biomedical analysis 08/2015; 117:73-78. DOI:10.1016/j.jpba.2015.08.027 · 2.98 Impact Factor

  • Biotechnology & Biotechnological Equipment 07/2015; DOI:10.1080/13102818.2015.1063452 · 0.30 Impact Factor
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    ABSTRACT: Tear breakup time (TBUT) is a useful technique for diagnosing dry eye disease (DED). The conventional method of measuring TBUT using fluorescein strips adds supplemental normal saline (NS) to the tear film (wet tear breakup time, WBUT) but does not represent the actual state of the tear film. We introduced a new TBUT method-dry tear breakup time (DBUT)-and investigated its reliability. We included DED patients with mild symptoms and a Schirmer test of 6 mm or more. Patients using tear substitutes and/or suffering from Sjogren's syndrome were excluded. For measuring DBUT, 1 μl of 5 % fluorescein solution was instilled on the end of an applicator using a micropipette. Once the fluorescein solution was dry, the applicator was sterilized using ethylene oxide gas, and the dried fluorescein was applied to the lower palpebral conjunctiva without supplemental NS. Agreement between the two tests and their sensitivity and specificity were analyzed. The average age of the patients was 32.1 ± 9.7 years (n = 124). The DBUT was lower than the WBUT, with a mean difference of -0.63 s. Both tests had a low statistically significant correlation with the Ocular Surface Disease Index. When the basis of definite diagnosis for DED was over grade 1 on the Oxford schema of staining, the cutoff value for the WBUT was 4.48 s and the sensitivity and specificity were 0.790 and 0.548 respectively. The area under the receiver operating characteristic (AUROC) curve was 0.609 (95 % confidence interval (CI): 0.517-0.695). When the cutoff value was 3.5 s for the DBUT, the sensitivity and specificity were 0.726 and 0.694 respectively, and the AUROC curve was 0.724 (95 % CI: 0.637-0.801). DBUT had a higher correlation with symptoms than the conventional WBUT. When DED was diagnosed with corneal or conjunctival staining based on the Oxford schema of staining, DBUT had a better accuracy than the conventional WBUT. DBUT may replace WBUT after confirming studies, especially when surface damage is to be detected as opposed to tear flow or symptoms.
    Albrecht von Graæes Archiv für Ophthalmologie 06/2015; 253(8). DOI:10.1007/s00417-015-3080-5 · 1.91 Impact Factor
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    ABSTRACT: Despite intensive research on photochemical activation of sol–gel metal oxide materials, the relatively long processing time and lack of deep understanding of the underlying chemical courses have limited their broader impact on diverse materials and applications such as thin-film electronics, photovoltaics, and catalysts. Here, in-depth studies on the rapid photochemical activation of diverse sol–gel oxide films using various spectroscopic and electrical investigations for the underlying physicochemical mechanism are reported. Based on the exhaustive chemical and physical analysis, it is noted that deep ultraviolet-promoted rapid film formation such as densification, polycondensation, and impurity decomposition is possible within 5 min via in situ radical-mediated reactions. Finally, the rapid fabrication of all-solution metal oxide thin-film-transistor circuitry, which exhibits stable and reliable electrical performance with a mobility of >12 cm2 V−1 s−1 and an oscillation frequency of >650 kHz in 7-stage ring oscillator even after bending at a radius of <1 mm is demonstrated.
    Advanced Functional Materials 03/2015; 25(19). DOI:10.1002/adfm.201500545 · 11.81 Impact Factor
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    ABSTRACT: Incorporation of Zr into AlOx matrix generates an intrinsically activated ZAO surface enabling formation of a stable semiconducting IGZO film and good interfacial property. Photochemically annealed metal-oxide devices and circuits with the optimized sol-gel ZAO dielectric and IGZO semiconductor layers demonstrate the high performance and electrically/mechanically stable operation of flexible electronics fabricate via low-temperature solution process. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Advanced Materials 01/2015; 27(7). DOI:10.1002/adma.201404296 · 17.49 Impact Factor
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    Nayoung Park · Byungjun Lee · Kyung Tae Kim · Hee Yong Youn ·

    01/2015; 3(3):167. DOI:10.2991/ijndc.2015.3.3.4
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    Kyung Tae Kim · Mi Young Choi ·
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    ABSTRACT: We intended to investigate the clinical features and success rates of surgery in premature children with exotropia.
    Journal of the Korean Ophthalmological Society 01/2015; 56(2):241. DOI:10.3341/jkos.2015.56.2.241
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    Kyung Tae Kim · Yeong Seong Eom · Injoon Son ·
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    ABSTRACT: Carbon nanotube/bismuth-selenium-tellurium composites were fabricated by consolidating CNT/Bi2(Se,Te)3 composite powders prepared from a polyol-reduction process. The synthesized composite powders exhibit CNTs homogeneously dispersed among Bi2(Se,Te)3 matrix nanopowders of 300 nm in size. The powders were densified into a CNT/Bi2(Se,Te)3 composite in which CNTs were randomly dispersed in the matrix through spark plasma sintering process. The effect of an addition of Se on the dimensionless figure-of-merit (ZT) of the composite was clearly shown in 3 vol.% CNT/Bi2(Se,Te)3 composite as compared to CNT/Bi2Te3 composite throughout the temperature range of 298 to 473 K. These results imply that matrix modifications such as an addition of Se as well as the incorporation of CNTs into bismuth telluride thermoelectric materials is a promising means of achieving synergistic enhancement of the thermoelectric performance levels of these materials.
    Journal of Nanomaterials 01/2015; 2015:1-6. DOI:10.1155/2015/202415 · 1.64 Impact Factor
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    ABSTRACT: Rotavirus is a major cause of acute gastroenteritis in young children in developed and developing countries. The use of probiotics for the treatment of gastrointestinal diseases is both safe and easily accessible. In this study, we evaluated the anti-rotaviral activities of probiotic mixtures in a Sprague-Dawley rat. 24 litters with their dams were randomly assigned to four groups; placebo, phosphate buffered saline (PBS), and two probiotic mixture (PRO-1 and PRO-2) groups. All rats were inoculated with rotavirus at dose of 8 log plaque forming units per rat at 5 days old. Animals in the PRO-1 and PRO-2 groups were orally administered probiotic mixtures 1 or 2, respectively, at a dose of 8 log colony forming units daily during 4 days. For control purposes, placebo and PBS groups were orally administered the same amount of placebo (containing maltose and polydextrose) or PBS once daily for 4 days, respectively. Antiviral analysis was performed by real-time quantitative PCR (RT-qPCR) and observing intestinal villi. As a result, weights of small intestines were greater in the PRO-1, PRO-2 groups than in control groups. Villi were short and villous epithelial necrosis was exhibited in control groups, but these morphological changes were not observed in PRO-1, PRO-2 treated rats. RT-qPCR analysis showed that VP7 gene level of rotavirus in fecal samples and small intestinal epithelial cells were lower in the PRO-1 and PRO-2 groups. These findings suggest that probiotic mixtures may be useful probiotics for the treatment of or as alternative therapies for rotaviral gastroenteritis.
    Korean Journal of Microbiology 12/2014; 50(4):296-301. DOI:10.7845/kjm.2014.4064

  • 12/2014; 10(4):177-182. DOI:10.11629/jpaar.2014.10.4.177
  • Gwang-Joo Jang · Kyung Tae Kim · Sangsun Yang · Yong-Jin Kim · Yong-Ho Park ·

    12/2014; 21(6):460-466. DOI:10.4150/KPMI.2014.21.6.460
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    ABSTRACT: The underwater applications of nanoenergetic materials (nEMs) could be extended by developing more convenient and reliable ignition methods. However, the underwater ignition of nEMs is a significant challenge because water perturbs the reactants prior to ignition and also quenches the subsequent combustion reaction of nEMs upon ignition. In this study, we developed flash-ignitable nEMs for underwater explosion. This was achieved by adding sea urchin-like carbon nanotubes (SUCNTs) as the optical igniter into an nEM matrix, composed of Al/CuO nanoparticles. The SUCNTs absorb the irradiated flash energy and rapidly convert it into thermal energy, and then the thermal energy is concentrated to ignite the core catalysts and neighboring nEMs. The maximum burn rate was achieved by adding 1 wt% SUCNTs into the nEM matrix. The burn rate significantly decreased with increasing amount of SUCNTs (⩾2 wt%), indicating that the remote flash ignition and controlled-explosion reactivity of nEMs are possible by incorporating an appropriate amount of SUCNTs.
    Combustion and Flame 12/2014; 162(4). DOI:10.1016/j.combustflame.2014.11.011 · 3.08 Impact Factor
  • Ho Jung · Jae Hong Park · Tae Hyun Kim · Hee Yeoun Kim · Kyung Tae Kim ·
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    ABSTRACT: The fabrication and evaluation of a wide-range vacuum gauge for monitoring the pressure level inside an infrared focal plane array and a wafer-level packaging (WLP) are reported. The proposed vacuum gauge has a microbolometer structure produced using the conventional surface micromachining process to achieve thermal isolation and high sensitivity. This structure has other advantages such as fast response time, a wider measurement range and easier integration to a Si substrate compared with other pressure sensors. The evaluation results show that the fabricated vacuum gauge has a linear dynamic range and a sensitivity of 10(-3) to 10(5) K/W/torr for vacuum pressures ranging from 10(-6) to 760 torr. Also, the response time to vacuum change is reduced from 0.11 s at 10(-5) torr to 15 ms at 100 torr. Therefore, the microbolometer-based vacuum gauge has good potential for application in WLP, and it is possible to hermetically seal it with various read-out integrated circuit substrates.
    Micro & Nano Letters 12/2014; 9(12):835-840. DOI:10.1049/mnl.2014.0378 · 0.85 Impact Factor
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    ABSTRACT: Capillary electrochromatography was employed for enantioseparation of α-amino acids and their derivatives. (-)-18-Crown-6-2,3,11,12-tetracarboxylic acid bonded on the silica was used as the chiral stationary phase and methanol/Tris-citric acid (20 mM, pH 3.0-4.5) (20:80, v/v) was used as mobile phase. The enantioseparation performance was discussed and structure-chiral separation relationship were tried to be explained. The enantiomeric resolution was increased when the pH of the mobile phase decreased or hydrogen of amino acid was substituted with halogen. The resolution of 4-bromophenylalanine was 2.37 at pH 4.5, however, this value was increased to 3.35 at pH 3.0. Bromo- or chloro-substituted phenylalanine tended to show higher resolution than fluoro-substituted one. For fluoro-substituted phenylalanine the resolution was increased in order of 4-, 3- and 2-substituted one. α-Methyltryptamine did not show reasonable separation. As the thermodynamic study is a useful tool to understand the chiral recognition, the temperature effect on the enantioseparation was studied and the thermodynamic parameters were calculated. The most important mechanism of chiral recognition for the analytes tested could be barrier effects based on the thermodynamic calculations. The coefficient of determination between hydrophobicity and separation factor was found to be 0.87, indicating favorable separation with higher hydrophobicity of amino acids.
    Archives of Pharmacal Research 11/2014; 38(8). DOI:10.1007/s12272-014-0507-1 · 2.05 Impact Factor
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    Bin Lee · Min Young Koo · Sung Hwan Jin · Kyung Tae Kim · Soon H. Hong ·
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    ABSTRACT: Reduced graphene oxide/alumina composite powders were prepared by mixing of graphene oxides and aluminum ions at the molecular-level. It was found that the composite consolidated from the powders showed that reduced graphene oxide were homogeneously dispersed and strongly bonded with the alumina matrix by oxygen atoms presenting at reduced graphene oxide/alumina interfaces. Both the hardness and the toughness of the composites were enhanced simultaneously by the addition of reduced graphene oxide, which act as bridges to restrain the propagation of cracks in the alumina matrix. It is clarified that graphenes can be utilized as promising reinforcements for enhancement in mechanical properties of ceramic materials when the molecular-level mixing process is applied.
    Carbon 11/2014; 78:212–219. DOI:10.1016/j.carbon.2014.06.074 · 6.20 Impact Factor
  • Byungha Choi · Ho-Sang Sohn · Kyung Tae Kim · Injoon Son ·

    10/2014; 47(5):215-220. DOI:10.5695/JKISE.2014.47.5.215
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    ABSTRACT: RNA interference (RNAi), mediated by small non-coding RNAs (e.g., miRNAs, siRNAs), influences diverse cellular functions. Highly complementary miRNA-target RNA (or siRNA-target RNA) duplexes are recognized by an Argonaute family protein (Ago2), and recent observations indicate that the concentration of Mg2+ ions influences miRNA targeting of specific mRNAs, thereby modulating miRNA-mRNA networks. In the present report, we studied the thermodynamic effects of differential [Mg2+] on slicing (RNA silencing cycle) through molecular dynamics simulation analysis, and its subsequent statistical analysis. Those analyses revealed different structural conformations of the RNA duplex in Ago2, depending on Mg2+ concentration. We also demonstrate that cation effects on Ago2 structural flexibility are critical to its catalytic/functional activity, with low [Mg2+] favoring greater Ago2 flexibility (e.g., greater entropy) and less miRNA/mRNA duplex stability, thus favoring slicing. The latter finding was supported by a negative correlation between expression of an Mg2+ influx channel, TRPM7, and one miRNA's (miR-378) ability to downregulate its mRNA target, TMEM245. These results imply that thermodynamics could be applied to siRNA-based therapeutic strategies, using highly complementary binding targets, because Ago2 is also involved in RNAi slicing by exogenous siRNAs. However, the efficacy of a siRNA-based approach will differ, to some extent, based on the Mg2+ concentration even within the same disease type; therefore, different siRNA-based approaches might be considered for patient-to-patient needs.
    PLoS ONE 10/2014; 9(10). DOI:10.1371/journal.pone.0109745 · 3.23 Impact Factor

Publication Stats

1k Citations
279.45 Total Impact Points


  • 2013-2015
    • Chungbuk National University
      • Department of Ophthalmology
      Chinsen, Chungcheongbuk-do, South Korea
    • National Institute of Environmental Research
      Sŏul, Seoul, South Korea
  • 2012-2015
    • Chung-Ang University
      • School of Electrical and Electronics Engineering
      Sŏul, Seoul, South Korea
  • 2007-2015
    • National Cancer Center Korea
      • • Molecular Epidemiology Branch
      • • Division of Cancer Biology
      QYK, Gyeonggi-do, South Korea
  • 2014
    • Sahmyook University
      Sŏul, Seoul, South Korea
  • 2013-2014
    • Korea Advanced Nano Fab Center
      화성시, Gyeonggi-do, South Korea
    • Pusan National University
      Busan, Busan, South Korea
  • 2012-2014
    • Chungnam National University
      • College of Pharmacy
      Daiden, Daejeon, South Korea
  • 2010-2014
    • Korea Institute of Materials Science
      Sŏngnam, Gyeonggi Province, South Korea
  • 2005-2014
    • Sungkyunkwan University
      • College of Information and Communication Engineering (SoICE)
      Sŏul, Seoul, South Korea
  • 2007-2013
    • Inje University Paik Hospital
      Sŏul, Seoul, South Korea
  • 1998-2013
    • Seoul National University
      • • Department of Materials Science and Engineering
      • • College of Medicine
      • • Department of Food and Nutrition
      Sŏul, Seoul, South Korea
  • 2006-2011
    • Korea Advanced Institute of Science and Technology
      • Department of Materials Science and Engineering
      Sŏul, Seoul, South Korea
  • 2005-2011
    • Yeungnam University
      Daikyū, Daegu, South Korea
  • 2008-2010
    • Konkuk University
      Sŏul, Seoul, South Korea
    • Hanyang University
      • Major in Obstetrics and Gynecology
      Sŏul, Seoul, South Korea
    • Inje University
      • College of Medicine
      Kŭmhae, Gyeongsangnam-do, South Korea
  • 2009
    • Chonnam National University
      • Division of Applied Bioscience and Biotechnology
      Gwangju, Gwangju, South Korea
    • Korea Institute of Science and Technology
      • Imaging Media Research Center
      Sŏul, Seoul, South Korea
    • Cheil General Hospital
      Sŏul, Seoul, South Korea
    • Catholic Kwandong University
      Gangneung, Gangwon-do, South Korea
  • 2004-2007
    • Gachon University
      • Department of Electrical Engineering
      Sŏngnam, Gyeonggi Province, South Korea
    • Seoul National University Hospital
      Sŏul, Seoul, South Korea
  • 2003
    • Dankook University
      Eidō, Chungcheongbuk-do, South Korea
  • 2001
    • Pohang University of Science and Technology
      • Department of Electrical and Computer Engineering
      Geijitsu, North Gyeongsang, South Korea
    • Yonsei University
      • Department of Electrical and Electronic Engineering
      Seoul, Seoul, South Korea