[Show abstract][Hide abstract] ABSTRACT: Blood Samples were kept at room temperature for a period of 3-6 months at room temperature to know the amount of quantitative DeoxyriboNucleic Acid [DNA] recovery from these samples. We are able to recover good amount of DNA for about first 3-6 weeks after which the DNA is decreased drastically and after two months there hardly any chance of intact DNA recovery from these samples. It has been concluded that blood samples recovered from scene of crime after about 1-2 months is a waste. The samples must be recovered as early as possible to recover intact DNA from them. The samples must be collected within 1-2 months from scene of crime until and unless the climate is cold enough to increases decay time. This study is very useful for the investigating authorities which can make errors while collecting blood samples for DNA analysis.
[Show abstract][Hide abstract] ABSTRACT: Blood Samples were kept at different temperature for a period of 3 months to know the amount of
quantitative DNA recovery from these samples in different conditions of temperature and ageing
[Time]. The DNA isolation was done by standard phenol-chloroform method and optical density of
the isolated DNA was measured by spectrophotometer. Recoverable DNA was found in all samples
even after 3 months of study however the amount of DNA recovery decreased with time which is
more marked in the initial phase followed by a slow and uniform decrease in DNA yield. Mean
DNA yield at zero time at room temperature from fresh blood samples was found to be 1027± 361
μg/ml. Minimum and maximum yield was found to be 432.7 μg/ ml and 1655 μg/ml respectively.