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Publications (4)2.74 Total impact

  • K. Shanmugapriya, D. Nityanandi, P.S. Saravana
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    ABSTRACT: Export Date: 18 October 2014
    International Journal of Drug Development & Research 01/2013; 5(3):155-168. · 0.18 Impact Factor
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    ABSTRACT: The cellulase producing bacteria was isolated from cow dung and characterized by morphological and biochemical analysis. Further partial purification of cellulase was carried out by dialysis and ammonium phosphate precipitation and also to determine molecular weight by SDS-PAGE. Bacterial isolated were grown on Carboxy Methyl Cellulose (CMC) agar at various optimum conditions such as parameters like pH, temperature, incubation period, carbon and nitrogen sources prior to examined and identified primarily as genus, Bacillus species for cellulase production by morphological and biochemical analysis. Optimized conditions of CMC and Coir waste as substrates were Glucose, Yeast extract of pH-6 at 40ºC for 48 hour but saw dust as substrate has optimized pH-7 with Xylose, Yeast extract at 50ºC for 48 hours. Among three substrates used, Carboxy Methyl Cellulose was found to be the best substrate for cellulase production, when compared to coir waste and saw dust as substrates. This is due to the less complexity and hence easy assimilation of Carboxy Methyl Cellulose by the isolated microbe. Ammonium sulphate precipitation followed by dialysis was performed to partially purify the cellulase enzyme. The molecular weight was found to be 32.5 kilodaltons by SDS-PAGE method. On performing zymogram staining, the cellulolytic property was confirmed. The use of microorganisms for the production of enzymes offers a promising approach for its large scale production and as a possible food supplement or in pharmaceutical industry. [I] INTRODUCTION Cellulose is the most abundant biomass on the earth. Cellulases are inducible enzymes which are synthesized by large number of microorganisms either cell-bound or extracellular during their growth on cellulosic materials [1]. Cellulose, acrystalline polymer of D-glucose residues connected by β-1, 4 glucosidic linkages, being the primary structural material of plant cell wall, is the most abundant carbohydrate in nature [2]. Therefore, it has become considerable economic interest to develop processes for effective treatment and utilization of cellulosic wastes as inexpensive carbon sources. Complete enzymatic hydrolysis of enzyme requires synergistic action of 3 types of enzymes, namely cellobiohydrolase, endoglucanase or carboxy methyl cellulose (CMCase) and α-glucosidases [3]. Cellulose is
    International Journal of Advanced Biotechnology and Research. 05/2012; 3(1):976-2612.
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    ABSTRACT: The present study was undertaken to describe the comparative evaluation of phytochemical constituents and antimicrobial activity of Artocarpus heterophyllus and Manilkara zapota seeds in four different solvents. A preliminary phytochemical analysis was carried out and concluded that the presence of alkaloids, tannins, phenolic compounds, flavonoids and saponins in both extract. The antimicrobial activity was tested using agar well diffusion method against six bacterial and four fungal strains and whereas, gentamycin and chloramphenical as antibiotic standards. The results showed that the antibacterial and antifungal activities were effective in ethanol, acetone and aqueous fractions of seed extract than ethyl acetate fractions in both samples. The MIC was determined in four different solvents of both extracts and reported that ethanol, acetone and aqueous extract possess minimum inhibition in both extract than ethylacetate. From the present study, it can be concluded that Artocarpus heterophyllus and Manilkara zapota seed extracts has effective antimicrobial activity and potent phytochemical constituents.
    Journal of Pharmacy Research. 01/2011; 4:2587-2589.
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    ABSTRACT: Consumption of diets rich in flavonoids is associated with reduced risk of cardiovascular diseases such as myocardial infarction. Cardiotoxicity was induced in rats by subcutaneous injection of isoproterenol at an interval of 24 h for 2 days. Isoproterenol-induced rats showed a significant increase in the levels of heart mitochondrial lipids, lipid peroxidation products, calcium and a significant decrease in the activities/levels of mitochondrial antioxidants, enzymes and adenosine triphosphate. Isoproterenol-induced rats also showed an increase in the intensities of serum lactate dehydrogenase-1 and 2 isoenzyme bands. Pretreatment with rutin at the dose of 80 mg/kg daily for 42 days to isoproterenol-induced rats prevented all the biochemical alterations. Transmission electron microscopic study also confirmed the protective effects of rutin on the structure of heart mitochondria. Thus, rutin reduced the extent of mitochondrial damage induced by isoproterenol and prevented cardiac mitochondrial dysfunction. The possible mechanisms for the observed effects of rutin could be due to scavenging free radicals, lowering lipid peroxides, lipids and calcium, improving multienzyme activities, glutathione levels, adenosine triphosphate levels, thereby improving cardiac mitochondrial structure and function. This study may have a significant impact on myocardial infarcted patients.
    Cardiovascular toxicology 09/2010; 10(3):181-9. · 2.56 Impact Factor