ABSTRACT: To investigate the effects of anti-HPV16-ribozyme on the cell proliferation and invasiveness of cultured cervical cancer cell line CaSKi.
CaSKi cells were transfected with anti-HPV16 E6-ribozyme and empty eukaryotic expression plasmids via lipofectin and designated as CaSKi-R and CaSKi-P cells respectively. The growth rate, cell colony-forming ability on soft agar, invasiveness and tumorigenicity of CaSKi-R, CaSKi-P, and CaSKi cells were studied using corresponding methods. The expressions of cox-2 and vascular endothelial growth factor (VEGF) mRNA of the 3 cell strains were determined with one-step reverse transcriptional PCR (RT-PCR), and immunocytochemistry was employed for detecting the expressions of COX-2 and VEGF antigens.
No distinct differences in the growth rate, colony-forming ability on soft agar, cell invasiveness and tumorigenicity were observed between CaSKi and CaSKi-P cells, whereas by comparison, CaSKi-R cells exhibited decreased growth rate, colony-forming ability, the cell invasiveness and tumorgenicity, with also lowered expression levels of cox-2 and VEGF mRNA as shown by RT-PCR analysis. Expressions of COX-2 and VEGF antigens were detected in all the 3 cell strains immunocytochemically, but compared with CaSKi and CaSKi-P cells, the antigen expressions in CaSKi-R cells were significantly weaker.
Anti-HPV16 E6-ribozyme may partially inhibit the proliferation and reduce the invasiveness of CaSKi cells possibly through decreasing cox-2 and VEGF expressions, which are the important agents for tumor invasion.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 01/2005; 24(12):1367-72.
ABSTRACT: To investigate the changes in the invasiveness of cervical cancer cell line CaSKi and the expression of vascular endothelial growth factor (VEGF) in response to treatment with anti-HPV16 E6-ribozyme, which plays a important role in the malignant phenotype and conversion of cervical cancer cells.
By means of lipofectin transfection, anti- HPV16 E6-ribozyme and empty eukaryotic expression plasmids were respectively transfected into CaSKi cell line (designated as CaSKi-R and CaSKi-P respectively). CaSKi, CaSKi-R and CaSKi-P cells were observed for their cell growth curves, clone forming ability on soft agar and tumorigenicity in nude mice. One-step reverse transcriptional PCR (RT-PCR) was employed to examine the expression of VEGF.
No significant differences were found in the growth rate, clone forming ability and tumorigenicity between CaSKi and CaSKi-P cells. In contrast, CaSKi-R exhibited obviously decreased growth rate, clone forming ability and tumorigenicity (P<0.05). RT-PCR analysis showed that the expression levels of VEGF mRNA in CaSKi-R cells were lower than those in CaSKi-P and CaSKi cells.
Anti-HPV16 E6-ribozyme may reduce the proliferative ability and invasiveness of cervical cancer cell line CaSKi, possibly through decreasing VEGF expression in CaSKi cells.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 06/2004; 24(5):529-32.
ABSTRACT: This study was designed to observe the effects of trocar puncture in the treatment of Bartholin gland cyst or abscess. The cure rate of these 22 cases was 100%, and no relapse of the disease occurred during the follow-up lasting for 5 to 12 months. Trocar puncture proves to be effective in the treatment of bartholin gland cyst or abscess, especially when the size is less than 3.0 cm.
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA 03/2003; 23(2):189.