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H Sugihara,
T Shibasaki,
A Tatsuguchi,
F Okajima,
S Wakita,
Y Nakajima,
K Tanimura,
H Tamura,
S Ishii,
J Kamegai,
H Akasu,
W Kitagawa, K Shimizu,
Y Nakamura,
E Uchida,
T Tajiri,
Z Naito,
H Katakami,
S Oikawa
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ABSTRACT: Cases of acromegaly due to GHRHproducing pancreatic endocrine tumors have been reported. Here we present a case of a 31-yr-old nonacromegalic man with hyperparathyroidism and elevated serum IGF-I with normal serum GH levels. Serum GH was not suppressed below 1 ng/ml by the glucose tolerance test and increased in response to TR H and GHRH administration. Magnetic resonance imaging (MRI) revealed pituitary hyperplasia and an abdominal computed tomography (CT ) scan showed a tumor in the pancreatic tail. Plasma concentration of GHRH was elevated. Based on these clinical data, multiple endocrine neoplasia (MEN) type 1 was suspected. Three enlarged parathyroid glands were removed and a distal pancreatectomy was performed. Pathological examination of the parathyroid glands and pancreatic tumor showed nodular hyperplasia and a well-differentiated endocrine tumor, respectively, both compatible with MEN features. Immunohistochemistry revealed positive immunoreactivity for GHRH, SS , insulin, glucagon, chromogranin A, and pancreatic polypeptide in the pancreatic tumor. After pancreatic surgery, elevated levels of GHRH and IGF-I were normalized and pituitary hyperplasia definitely decreased in size. In cases of pituitary hyperplasia with elevated IGF-I, ectopic GHRH syndrome must be considered even if physical features of acromegaly are absent. It is also important to measure plasma GHRH concentrations in order to give a diagnosis.
Journal of endocrinological investigation 06/2007; 30(5):421-7. · 1.57 Impact Factor
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Journal of Surgical Oncology 08/2001; 77(3):217-20. · 2.10 Impact Factor
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Journal of Nippon Medical School 05/2000; 67(2):134-8.
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ABSTRACT: Most patients with thyroid carcinoma have a good prognosis. Due to the small number of fatal cases, it has not been clarified what conditions result in death for patients with thyroid carcinoma. To provide appropriate management for advanced thyroid carcinoma patients, we analyzed causes of death in 161 fatal cases. Clinical characteristics and immediate (final) causes of death based on pathological conditions were analyzed in 62 anaplastic carcinomas and 99 fatal differentiated carcinomas. Single fatal conditions could not be specified in 55 patients. In the remaining 106 patients, respiratory insufficiency (43%) was the most common specific fatal condition, followed by circulatory failure (15%), hemorrhage (15%), and airway obstruction (13%). Respiratory insufficiency due to remarkable pulmonary metastasis replacing lung tissue, massive hemorrhage and airway obstruction due to uncontrolled local tumors, and circulatory failure resulting from compression of the vena cava by extensive mediastinal or sternal metastases were found to be the most important immediate causes of death. Based on this knowledge, several palliative procedures may be worth considering to improve survival and quality of life in patients with advanced thyroid carcinoma.
Journal of Clinical Endocrinology & Metabolism 12/1999; 84(11):4043-9. · 6.50 Impact Factor
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ABSTRACT: An experimental study was performed to investigate the influence of hypothyroidism on wound healing. A state of severe hypothyroidism was induced initially by performing a total thyroidectomy on rat models; subsequently wounds were made by making long midline abdominal incisions and then suturing them. The parameters used to evaluate the process of wound healing of these incisions were the assay of type-I collagen, type-III collagen (procollagen peptide PIPC and P III P, each being the precursor of collagen), type-IV collagen, and hydroxyproline. The assays were repeated at specific times and compared to assays of similar parameters taken from a control group. In the state of hypothyroidism, a decrease was observed in type-IV collagen and hydroxyproline during the proliferative phase of wound healing. This indicated that the state of hypothyroidism constitutes an important factor in delaying wound healing.
Nippon Ika Daigaku Zasshi 07/1999; 66(3):176-80.
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Journal of the American College of Surgeons 07/1999; 188(6):697-703. · 4.55 Impact Factor
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Journal of Surgical Oncology 12/1998; 69(3):178-80. · 2.10 Impact Factor
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Nippon Ika Daigaku Zasshi 09/1998; 65(4):320-3.
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ABSTRACT: Germ-like and somatic mutations in the RET proto-oncogene are associated with inherited and sporadic medullary thyroid carcinoma (MTC). The majority of patients with multiple endocrine neoplasia type 2A (MEN2A) and familial medullary thyroid carcinoma (FMTC) carry germ-line point mutations that result in the substitution of one of five cysteine residues. We investigated exons 10, 11, 13, 14 and 16 of the RET proto-oncogene in 33 unrelated Japanese patients with MTC. Eleven of the 33 cases (33%) were found to have germ-line mutations. Three previously unreported mutations in exon 10 and 11 were identified: one in codon 620, (TGC-->GGC), resulting in a cysteine to glycine substitution, and two in codon 630, (TGC-->TCC) and (TGC-->TAC), resulting in cysteine to serine and cysteine to tyrosine changes, respectively. The new mutations were present in the germ-line DNA of four unrelated patients for whom a family history of MTC had not been documented. Because the new RET alleles described here involve cysteine residues in a region of protein previously associated with FMTC and MEN2A, it is very likely that they represent mutations that predispose to the development of MTC.
Oncogene 06/1997; 14(25):3103-6. · 6.37 Impact Factor
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Nippon Ika Daigaku Zasshi 07/1996; 63(3):228-32.
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Nippon Ika Daigaku Zasshi 11/1995; 62(5):535-8.
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Nippon Ika Daigaku Zasshi 12/1994; 61(6):638-41.
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Nippon Ika Daigaku Zasshi 11/1994; 61(5):515-8.
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ABSTRACT: A new chemosensitivity test was evaluated by the MTT colorimetric asay with human tumor cell lines encapsulated in alginate microcapsules with semipermeable membranes. The proliferation of KATOIII in the microcapsules rapidly increased on the 4th day after the encapsulation. The change expressed on the proliferation curve of the encapsulated KATOIII was approximately 2 days behind the proliferation of the suspension culture. The encapsulated cell number reversed and further proliferation was recognized after the 12th day. After the incubation for 5 hours of encapsulated KATOIII with the medium supplemented with 0.5% MTT, a blue formazan crystal formation was observed radiating around the cells in the capsules. MTT assay depends on the cellular reduction of the absorbance spectra at 540 nm (OD540nm), for complete solubilization of the formazan by DMSO. The formazan formation was observed more significantly in serum medium culture than in serum free medium. In MIT assay when 0.1 mol succinic acid was added, OD540nm of encapsulated KATOIII increased by approximately 50% and its sensitivity also increased greatly. In comparison the results of MTT assay for encapsulated KATOIII and MKN28 with suspended cells under the same conditions (0.1, 1, 10 micrograms/ml of MMC and ADR, 0.5, 5, 50 micrograms/ml of 5FU, 10, 30, 50 micrograms/ml of CDDP), the calculated inhibition index (%) with encapsulated cells were similar to the percentages obtained in the former MTT assay. In this study with microcapsules, the formazan formation in the capsules and the absorbance were macroscopically inhibited when the drug concentration was increased. The encapsulated KATOIII, which was implanted intraperitoneally into rat with a 16-gauge needle, was recovered at a rate of 70.8% on the 8th day and at a rate of 54.5% on the 16th day. The recovered encapsulated KATOIII proliferated remarkably forming cell clots on the 8th day after implantation. Incubation with MTT promoted formazan formation and sufficient cell viability was recognized. The Tegafur concentration in the intraperitoneal microcapsules and the microcapsules containing KATOIII after the intravenous administration of Tegafur was similar to the intrahepatic level. The 5FU level in the microcapsules containing KATOIII was higher than that in the capsules alone. In an attempt to conduct an in vivo chemosensitivity test, encapsulated KATOIII and MKN28 were intraperitoneally implanted, 4 mg/kg of MMC, ADR and CDDP, and 75mg/kg of 5FU were intravenously administered on the 2nd and 4th days after the implantation. On the 6th day, MTT assay was performed on the recovered microcapsules containing cells and the inhibition index was calculated.(ABSTRACT TRUNCATED AT 400 WORDS)
Nippon Ika Daigaku Zasshi 11/1994; 61(5):422-34.
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ABSTRACT: We have studied the cryopreservation of thyroid pieces for the purpose of autotransplantation. In this paper, the optimal freezing condition was investigated by comparing the survival cells of the cryopreserved thyroid pieces under various freezing conditions, including cooling rate, freezing medium, cryoprotectant, size of pieces and prefreezing incubation. The recovery regarded as survival rate was also evaluated. Based on the results, the optimal freezing condition was that 1 mm 3 of the thyroid pieces in the freezing medium, consisted of culture medium supplemented with 10% of fetal bovine serum and 10% of dimethylsulfoxide, were cooled slowly until -80 degrees C with prefreezing incubation at 4 degrees C for 1 hour and kept in liquid nitrogen for preservation. The number of survival cells under the optimal condition was 1.24-2.03 (1.71 +/- 0.40) x 10(6)/0.1 g tissue, and recovery was 25.2-58.0 (45.5 +/- 14.6)%. This high survival rate suggests the possibility that the cryopreserved thyroid pieces are utilized as the autografts maintaining the intact follicles.
Nippon Geka Gakkai zasshi 02/1994; 95(1):14-20.
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Nippon Ika Daigaku Zasshi 01/1994; 60(6):411-4.
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Nippon Ika Daigaku Zasshi 11/1993; 60(5):360-1.
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Nippon Ika Daigaku Zasshi 09/1992; 59(4):355-6.
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K Shimizu
Nippon Ika Daigaku Zasshi 03/1992; 59(1):66-9.
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Nippon Geka Gakkai zasshi 01/1992; 92(12):1728.
