-
[show abstract]
[hide abstract]
ABSTRACT: PURPOSE: As a newly added member of the UNC5H receptors, the function of UNC5D/H4 in tumorigenesis remains poorly defined. The aim of this study was to examine the expression of UNC5D in primary renal cell carcinomas (RCC), analyze the mechanisms responsible for its down-regulation in RCC and assess its functional relevance to tumor growth and migration. EXPERIMENTAL DESIGN: 44 paired primary RCC and corresponding adjacent non-cancerous tissues were collected. The mRNA and protein expression level of UNC5D was assessed by RT-PCR, real-time PCR or immunohistochemistry. Epigenetic alterations in UNC5D promoter and loss of heterozygosity (LOH) in the UNC5D locus were also analyzed. Ectopic expression of UNC5D in renal cancer cells with silenced expression of UNC5D was used for analysis of the biological functions of UNC5D. RESULTS: UNC5D expression was attenuated in multiple carcinoma cell lines including renal cancer cells. Similar reduction was also observed in primary RCC tissues as compared to paired adjacent non-cancerous tissues. Methylation specific PCR showed hypermethylation in UNC5D promoter in a significant proportion (18 out of 44) of tumor tissue (40.9%). LOH of UNC5D was observed in 13 out of 44 RCC patients (29.5%). Restoration of UNC5D expression in renal cancer cells significantly inhibited cell proliferation, anchorage dependent and independent growth as well as migration and invasion. While knockdown of UNC5D promoted cell growth. Furthermore, ectopic expression of UNC5D induced G2/M cell cycle arrest. CONCLUSIONS: UNC5D is a functional tumor suppressor that is frequently down-regulated in RCC due to promoter hypermethylation and loss of heterozygosity.
Clinical Cancer Research 04/2013; · 7.74 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Along with the improvement of diagnostic techniques, hepatitis E has attracted increasing awareness in recent years. Hepatitis E virus infection leads to high mortality in pregnant women and patients with underlying liver disease. Several hepatitis E vaccine candidates have been designed and have proved their efficacy in animal models; two candidates have successfully undergone clinical trials. Having proved safe and effective in a large phase III trials, an Escherichia coli expressed particulate protein, HEV 239, has been registered in China and is now available for use in China.
Seminars in Liver Disease 02/2013; 33(1):79-88. · 7.05 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A Gram-negative, non-spore-forming, rod-shaped Flavobacterium-like bacterial strain, hgT, was isolated from soil, and subjected to a polyphasic taxonomic study. Strain hgT grew optimally at pH 7.0 and 30°C in the presence of 1 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C15:0 and iso-C17:0 3-OH as the major fatty acids. The DNA G+C content was 34 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain hgT belonged to the genus Flavobacterium. Levels of 16S rRNA gene sequence similarity between strain hgT and the type strains of Flavobacterium species were below 94.7 %. Strain hgT differed from phylogenetically related Flavobacterium species in several phenotypic characteristics. On the basis of phenotypic and phylogenetic distinctiveness, hgT (=CCTCC AB 2012099T = KACC 16855T) was classified in the genus of Flavobacterium as the type strain of a novel species, for which the name Flavobacterium yanchengense sp. nov. is proposed.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 01/2013; · 2.11 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Mature HIV-1 viral particles assemble as a fullerene configuration comprising p24 capsid hexamers, pentamers and dimers. In this paper, we report the X-ray crystal structures of the p24 protein from natural HIV-1 strain (BMJ4) in complex with Fab A10F9, which recognizes a conserved epitope in the C-terminal domain of the BMJ4 p24 protein. Our structures reveal a novel shoulder-to-shoulder p24 dimerization mode that is mediated by an S-S bridge at C177. Consistent with these structures, the shoulder-to-shoulder dimer that was obtained from the BMJ4 strain was also observed in p24 proteins from other strains by the introduction of a cysteine residue at position 177. The potential biological significance was further validated by the introduction of a C177A mutation in the BMJ4 strain, which then displays a low infectivity. Our data suggest that this novel shoulder-to-shoulder dimer interface trapped by this unique S-S bridge could represent a physiologically relevant mode of HIV-1 capsid assembly during virus maturation, although Cys residue itself may not be critical for HIV-I replication.
PLoS ONE 01/2013; 8(4):e61314. · 4.09 Impact Factor
-
Wei Wang,
Tong Cheng,
Ke Ma,
Dezhen Xia,
Yongmei Wang,
Jian Liu,
Hailian Du,
James Wai Kuo Shih, Jun Zhang,
Qinjian Zhao,
Ningshao Xia
[show abstract]
[hide abstract]
ABSTRACT: The baculovirus expression vector system (BEVS) is one of the most powerful methods for production of recombinant proteins for research or commercial purposes. Titration of viable virus in insect cell culture is often required when BEVS is used for basic research or bioprocessing. An enzyme-linked immunosorbent spot (ELISPOT) assay using monoclonal antibodies against the major capsid protein VP39 of both Autographa californica nuclear polyhedrosis virus (AcMNPV) and Bombyx mori nuclear polyhedrosis virus (BmNPV) was developed for baculovirus quantitation at 48h post-infection. The titer was determined by visualizing infected insect cells as blue spots and automated spot counting was achieved with ELISPOT hardware and software. Log-scale comparison of the results between the ELISPOT assay and a conventional end point dilution assay using a fluorescent marker showed a good correlation for both AcMNPV (R(2)=0.9980, p<0.05) and BmNPV (R(2)=0.9834, p<0.05). In conclusion, a novel, rapid and semi-automated procedure for titrating baculovirus was developed based on the specific immunostaining of infected cells followed by automated spot counting.
Journal of virological methods 12/2012; · 2.13 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The hepatitis E virus (HEV) capsid protein has been demonstrated to be able to assemble into particles in vitro. However, this process and the mechanism of protein-protein interactions during particle assembly remain unclear. In this study we investigated the assembly mechanism of HEV structural protein subunits, the capsid protein p239 (aa368-606), using analytical ultracentrifugation. It was the first to observe that the p239 can form particles in 4 M urea as a result of supplementation with salt, including ammonium sulfate [(NH(4) )(2) SO(4) ], sodium sulfate (Na(2) SO(4) ), sodium chloride (NaCl), and ammonium chloride (NH(4) Cl). Interestingly, it is the ionic strength that determines the efficiency of promoting particle assembly. The assembly rate was affected by temperature and salt concentration. When (NH(4) )(2) SO(4) was used, assembling intermediates of p239 with sedimentation coefficient values of approximately 5 S, which were mostly dodecamers, were identified for the first time. A highly conserved 28-aa region (aa368-395) of p239 was found to be critical for particle assembly, and the hydrophobic residues Leu(372) , Leu(375) , and Leu(395) of p239 was found to be critical for particle assembly, which was revealed by site-directed mutagenesis. This study provides new insights into the assembly mechanism of native HEV, and contributes a valuable basis for further investigations of protein assembly by hydrophobic interactions under denaturing conditions.
Protein Science 12/2012; · 2.80 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Heterogeneous nuclear ribonucleoproteins (hnRNPs), including hnRNP H, are RNA-binding proteins that function as splicing factors and are involved in downstream gene regulation. hnRNP H, which binds to G triplet regions in RNA, has been shown to play an important role in regulating the staged expression of late proteins in viral systems. Here, we report that the specific association between hnRNP H and a late viral capsid protein, human papillomavirus (HPV) L1 protein, leads to the suppressed function of hnRNP H in the presence of the L1 protein. The direct interaction between the L1 protein and hnRNP H was demonstrated by complex formation in solution and intracellularly using a variety of biochemical and immunochemical methods, including peptide mapping, specific co-immunoprecipitation and confocal fluorescence microscopy. These results support a working hypothesis that a late viral protein HPV16 L1, which is down regulated by hnRNP H early in the viral life cycle may provide an auto-regulatory positive feedback loop that allows the rapid production of HPV capsid proteins through suppression of the function of hnRNP H at the late stage of the viral life cycle. In this positive feedback loop, the late viral gene products that were down regulated earlier themselves disable their suppressors, and this feedback mechanism could facilitate the rapid production of capsid proteins, allowing staged and efficient viral capsid assembly.
Biochemical and Biophysical Research Communications 12/2012; · 2.48 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The hexadecyltrimethylammonium bromide (HDTMAB) immobilized hollow mesoporous silica spheres were prepared for the efficient removal of perfluorooctane sulfonate (PFOS) from aqueous solution. Besides the traditional sorption behavior including sorption kinetics as well as effect of solution pH and temperature, the effect of increasing volume which simulated the natural river where the rate of solute and solvent was relatively constant and solution volume was always changing was investigated. The result indicated that the residual PFOS concentrations in aqueous phase decreased with increasing solution pH and ionic strength, whereas they increased with increasing temperature. The HDTMAB immobilized material still maintained high efficiency after increasing volume, that is, the removal kept more than 99% after the treatment when the initial PFOS concentration was 1mgL(-1). The uptake behavior and morphology of spheres which was characterized by transmission electron microscopy (TEM) revealed that the additional HDTMAB and mesoporous shell were responsible for the enhanced sorption of PFOS. It was concluded that electrostatic interaction and Ca-bridge role played an important role in the sorption of PFOS on the mesoporous SiO(2) hollow spheres, whereas, hydrophobic interaction contributed to the nice sorption performance of PFOS on the HDTMAB immobilized sorbent.
Chemosphere 12/2012; · 3.21 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: OBJECTIVE: To characterize complications of pregnancy, labor and delivery associated with maternal asthma in a contemporary US cohort. STUDY DESIGN: A retrospective cohort based on electronic medical record data from 223,512 singleton deliveries from 12 clinical centers across the United States between 2002-2008. RESULTS: Women with asthma had higher odds of preeclampsia (adjusted odds ratio (aOR)=1.14; 95% confidence interval (95%CI)=1.06-1.22), superimposed preeclampsia (aOR=1.34; 95%CI=1.15-1.56), gestational diabetes (aOR=1.11; 95%CI=1.03-1.19), placental abruption (aOR=1.22; 95%CI=1.09-1.36), and placenta previa (aOR=1.30; 95%CI=1.08-1.56). Asthmatic women had a higher odds of preterm birth overall (aOR=1.17; 95%CI=1.12-1.23) and of medically-indicated preterm delivery (aOR=1.14; 95%CI=1.01-1.29). Asthmatics were less likely to have spontaneous labor (aOR=0.87; 95%CI=0.84-0.90) and vaginal delivery (aOR=0.84; 95%CI=0.80-0.87). Risks were higher for breech presentation (aOR=1.13; 95%CI=1.05-1.22), hemorrhage (aOR=1.09; 95%CI=1.03-1.16), pulmonary embolism (aOR=1.71; 95%CI=1.05-2.79), and maternal ICU admission (aOR=1.34; 95%CI=1.04-1.72). CONCLUSION: Maternal asthma increased risk for nearly all outcomes studied in a general obstetric population.
American journal of obstetrics and gynecology 11/2012; · 3.28 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: There is emerging evidence for an under-recognized hepatitis E virus (HEV) as a human pathogen. Among different reasons for this neglect are the unsatisfactory performance and under-utilization of commercial HEV diagnostic kits; for instance, the number of anti-HEV IgM kits marketed in China is about one-fifth of that of hepatitis A kits. Over the last two decades, substantial progress has been achieved in furthering our knowledge on the HEV-specific immune responses, antigenic features of HEV virions, and development of serological assays and more recently prophylactic vaccines. This review will focus on presenting the evidence of the importance of HEV infection for certain cohorts such as pregnant women, the key antigenic determinants of the virus, and immunogenicity and clinical efficacy conferred by a newly developed prophylactic vaccine. Robust immunogenicity, greater than 195-fold and approximately 50-fold increase of anti-HEV IgG level in seronegative and seropositive vaccinees, respectively, as well as impressive clinical efficacy of this vaccine was demonstrated. The protection rate against the hepatitis E disease and the virus infection was shown to be 100 % (95 % CI 75-100) and 78 % (95 % CI 66-86), respectively.
Journal of Gastroenterology 11/2012; · 4.16 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: The taxonomic status of a bacterium strain DC-8T, isolated from activated sludge, was determined using a polyphasic taxonomic approach. The cells of strain DC-8T were Gram-negative, non-motile, non-spore-forming and rod-shaped. The isolate grew at temperature range of 10-40°C (optimum 30-35 °C), pH range of 5.0-10.0 (optimum 6.5-8.0) and NaCl 0-5 % (optimum 0-1 %). The predominant menaquinone of strain DC-8T was MK-7 and major fatty acids were summed features 3 (C16:1 ω6c and/or C16:1 ω7c; 39.7 %), iso-C15:0 (33.7 %) and C16:0 (5.2 %). The DNA G+C content was 39.8 mol %. Phylogenetic analysis based on 16S rRNA gene sequences comparison revealed that strain DC-8T was a member of the genus Sphingobacterium. Strain DC-8T shared the highest similarity with S. siyangense SY1T (98.4 %), S. multivorum IAM14316T (98.3 %), S. canadense CR11T (98.0 %) and S. detergens 6.2ST (97.9 %), and shared less than 97 % similarities with other Sphingobacterium species. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain DC-8T and its closest phylogenetic neighbours were below 70 %. Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain DC-8T was clearly distinguished from all recognized Sphingobacterium species and should be classified as a novel species of the genus Sphingobacterium, for which the name Sphingobacterium caeni sp. nov. is proposed. The type strain is DC-8T (=CCTCC AB 2012020T =KACC 16850T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2012; · 2.11 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A novel biosurfactant-producing strain, designated as YW1T, was isolated from agricultural soil. Its taxonomic position was investigated using a polyphasic approach. The cells were short rods, Gram-negative, non-sporulating and motile. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YW1T was a member of the genus Comamonas, and showed highest sequence similarities to C. aquatica LMG 2370T (98.5 %), C. kerstersii LMG 3475T (97.7 %), and C. terrigena LMG 1253T (97.7 %). Furthermore, the results of DNA-DNA hybridization experiments against these three strains were clearly lower than 70% DNA-DNA relatedness, and consequently confirmed that this new strain does not belong to a previously described species of the genus Comamonas. The major respiratory quinone was ubiquinone-8. The major fatty acids (> 5 %) were C16:0 (30.1 %), summed feature 3 (C16:1ω6c and/or C16:1ω7c; 25.4 %), summed feature 8 (C18:1ω6c and/or C18:1ω7c; 15.3 %), C17:0 cyclo (7.4 %) and C14:0 (5.8 %). The major polar lipids are DPG (diphosphatidylglycerol); PG (phosphatidylglycerol); PE (phosphatidylethanolamine); PL (unknown phospholipids); L (unknown lipids). Based on the phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics, strain YW1T was clearly distinguished from all recognized Comamonas species and should be classified as a novel species of the genus Comamonas, for which the name Comamonas jiangduensis sp. nov. is proposed. The type strain is YW1T (=CCTCC AB 2012033T =KACC 16697T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2012; · 2.11 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Metarhizium anisopliae is one of the most common species of entopathogenic fungi. It has economic and social benefits in many countries where used in agriculture as an important biological control agent of insect pests. M. anisopliae can exist as multiple cell types, which suggests that this fungus has a complex way of gene regulation. MicroRNAs (miRNAs) are endogenous small noncoding RNAs. They play a crucial role in the regulation of gene expression and cell function in plants, animals, and in fungi where they were termed miRNA-like RNAs (milRNAs). In this study, we aimed to identify potential milRNAs in M. anisopliae that may regulate the processes of mycelium growth and conidiogenesis (CO). Two small RNA (sRNA) libraries were constructed and submitted to Solexa sequencing. Fifteen milRNAs were identified using deep-sequencing and computational analysis; most of these milRNAs originated from single genes. Database searches revealed that these novel milRNAs had no homologues in other organisms and were, therefore, M. anisopliae-specific. Many of the milRNAs had differential expression profiles for either mycelium growth or CO. The expression of the selected milRNAs was validated by quantitative reverse transcription polymerase chain reaction. Seventy-eight potential target mRNAs for 14 of the milRNAs were identified successfully by computational analysis. These milRNAs may play an important role in the regulation of mycelial growth and conidiation in M. anisopliae. To our knowledge, this study is the first report of milRNA profiles of organisms in the order Hypocreales. This information could be used to study the regulation of genes and their networks in M. anisopliae.
Fungal Biology 11/2012; 116(11):1156-62. · 1.43 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this paper we study robust pulse design for electron shuttling in solid
state devices. This is crucial for many practical applications of coherent
quantum mechanical systems. Our objective is to design control pulses that can
transport an electron along a chain of donors, and also make this process
robust to parameter uncertainties. We formulate it as a set of optimal control
problems on the special unitary group SU(n), and derive explicit expressions
for the gradients of the aggregate transfer fidelity. Numerical results for a
donor chain of ionized phosphorus atoms in bulk silicon demonstrate the
efficacy of our algorithm.
10/2012;
-
[show abstract]
[hide abstract]
ABSTRACT: In recent year, Enterovirus 71 (EV71)-associated hand, foot and mouth disease (HFMD) has become an important public health issue in China. EV71 has been classified into genotypes A, B1-B5 and C1-C5. With such genetic diversity, whether the convalescent or recovery antibody responses can cross-protect infections from other genotypes remains a question. Understanding of the antigenicity of such diverse genetic EV71 isolates is crucial for the EV71 vaccine development. Here, a total of 186 clones anti-EV71 MAbs was generated and characterized with Western blot and cell-based neutralization assay. Forty neutralizing anti-EV71 MAbs were further used to analyze the antigenic properties of 18 recent EV71 isolates representing seven genotypes in neutralization assay. We found that most neutralizing anti-EV71 MAbs are specific to conformational epitopes. We also classified the 40 neutralizing anti-EV71 MAbs into two classes according to their reactivity patterns with 18 EV71 isolates. Class I MAb can neutralize all isolates, suggesting conserved epitopes are present among EV71. Class II MAb includes four subclasses (IIa-IId) and neutralizes only subgroups of EV71 strains. Conversely, 18 EV71 strains were grouped into antigenic types 1 and four antigenic subtypes (2.1-2.4). These results suggest that the current genotyping of EV71 does not reflect their antigenicity which may be important in the selection of EV71 vaccine strains. This panel of neutralizing anti-EV71 MAbs may be useful for the recognition of emerging antigenic variants of EV71 and vaccine development.
Vaccine 10/2012; · 3.77 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: Lipid rafts have been shown to play significant roles in thymocyte development. However, the exact role of lipid rafts in single positive (SP) thymocyte differentiation is poorly characterized. We previously defined a developmental program (SP1→SP4) for CD4SP thymocytes. In this study, we found that lipid raft components were up-regulated during CD4SP maturation. Qa-2, a unique marker for the most mature SP4 subset, was localized to lipid rafts and heterogeneously expressed in SP4 cells. Functional assays showed that the proliferation capacity of SP4 cells correlated with the expression of Qa-2. Raft-disruption on both CD4SP and epithelial cells by cholesterol extraction or cholesterol oxidation in a medullary thymic epithelial cell (mTEC)-supported co-culture system impaired the transition from SP3 to SP4. This result was further confirmed in fetal thymic organ culture system. Collectively, these studies suggest that raft-associated signaling between mTECs and thymocytes drives the differentiation of CD4SP thymocytes and lipid rafts are involved in the final maturation of SP4 thymocytes.
Immunology letters 10/2012; · 2.91 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: In this paper we apply optimal control technique to derive the control fields
that transfer electron from one end of a chain of donors or quantum dots to the
other. We formulate it as an optimal steering problem, and then derive the
dynamics of the optimal control. A numerical algorithm is developed to
efficiently generate control pulses. We apply this technique to transfer an
electron between sites of a triple quantum dot and an ionized chain of
phosphorus dopants in silicon, in which we show that a 500 G magnetic field
will transfer the electron and two of the four hyperfine states of the
electron-nuclear spin pair.
08/2012;
-
[show abstract]
[hide abstract]
ABSTRACT: A porous NiO/RGO hybrid film is prepared by the combination of electrophoretic deposition and chemical-bath deposition. The porous hybrid film exhibits a noticeable electrochromism with reversible color changes from transparent to dark brown, and shows high coloration efficiency (76 cm(2) C(-1)), fast switching speed (7.2 s and 6.7 s) and better cycling performance compared with the porous NiO thin film. The enhancement of electrochromic performances are attributed to the reinforcement of the electrochemical activity of the RGO sheets and the greater amount of open space in the porous hybrid film which allows the electrolyte to penetrate and shorten the proton diffusion paths within the bulk of NiO.
Nanoscale 08/2012; 4(18):5724-30. · 5.91 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: A Gram-negative, aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated DC-3T, was isolated from activated sludge of a wastewater treatment plant in China, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain DC-3T belongs to the family Xanthomonadaceae and formed a subclade with the genus Dokdonella. Strain DC-3T shared the highest similarity with Dokdonella soli KACC 12741T (97.1 %) and Dokdonella fugitiva KACC 13124T (97.1 %). The G + C content of the genomic DNA was 71.5 mol %. The major respiratory quinone was Q-8 and the major fatty acids were iso-C17 : 1ω9c (31.6 %), iso-C15 : 0 (12.6 %), iso-C16 : 0 (21.3 %), iso-C17 : 0 (13.1 %) and isoC11:0 3-OH (6.5 %) supporting the affiliation of strain DC-3T to the genus Dokdonella. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness values between strain DC-3T and its closest phylogenetic neighbours were below 30 %. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain DC-3T from recognized species of the genus Dokdonella. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain DC-3T represents a novel species of the genus Dokdonella, for which the name Dokdonella kunshanensis sp. nov. is proposed. The type strain is DC-3T (=CCTCC AB2011179T= KACC 16511T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2012; · 2.11 Impact Factor
-
[show abstract]
[hide abstract]
ABSTRACT: We sought to study neonatal outcomes in early preterm births by delivery route.
Delivery precursors were analyzed in 4352 singleton deliveries, 24 0/7 to 31 6/7 weeks' gestation. In a subset (n = 2906) eligible for a trial of labor, neonatal mortality in attempted vaginal delivery (VD) was compared to planned cesarean delivery stratified by presentation.
Delivery precursors were classified as maternal or fetal conditions (45.7%), preterm premature rupture of membranes (37.7%), and preterm labor (16.6%). For vertex presentation, 79% attempted VD and 84% were successful. There was no difference in neonatal mortality. For breech presentation, at 24 0/7 to 27 6/7 weeks' gestation, 31.7% attempted VD and 27.6% were successful; neonatal mortality was increased (25.2% vs 13.2%, P = .003). At 28 0/7 to 31 6/7 weeks' gestation, 30.5% attempted VD and 17.2% were successful; neonatal mortality was increased (6.0% vs 1.5%, P = .016).
Attempted VD for vertex presentation has a high success rate with no difference in neonatal mortality unlike breech presentation.
American journal of obstetrics and gynecology 08/2012; 207(2):117.e1-8. · 3.28 Impact Factor
