Jun Yang

Wuhan Children's Hospital, Wu-han-shih, Hubei, China

Are you Jun Yang?

Claim your profile

Publications (11)28.41 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: To report a laparoscopic approach for pediatric inguinal hernia repair using a hybrid single-incision laparoscopic (H-SIL) technique and its clinical outcomes. A retrospective study was carried out in inguinal hernia cases treated with the new H-SIL approach using intracorporeal jumping purse-string sutures. The operative time, length of postoperative hospital stay, efficiency of the operation, and complications were analyzed. In total, 157 inguinal high ligations were performed in 106 children (89 boys, 17 girls). The median age was 1.5 years (range, 25 days-11.6 years). The mean operative time was 15.8±3.4 minutes for the single-side procedure and 20.3±2.5 minutes for bilateral procedures. The mean postoperative hospital stay was 0.99±0.52 (range, 0.25-3 days). No postoperative bleeding, abdominal wall emphysema, abdominal viscera injury, or scrotal edema was found, and there were no known cases of postoperative testicular atrophy or hypotrophy. Ninety-three percent of the patients became fully mobile on the first postsurgical day. The median follow-up period was 17 months (range, 9-21 months), with no recurrence, no visible scars on the abdominal wall, and no foreign body felt in the inguinal region. This H-SIL approach is a safe and efficient method for pediatric inguinal hernia repair. The maneuverability is the same as that in the triport laparoscopic technique, and the cosmetic results are similar to those of single-port laparoscopic surgery.
    Journal of Laparoendoscopic & Advanced Surgical Techniques 06/2015; 25(8). DOI:10.1089/lap.2014.0474 · 1.34 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to investigate the role of Th17 and Treg cells in biliary atresia (BA) and to assess the liver cytokine environment in BA patients. The percentages of Th17 and Treg cells in peripheral blood mononuclear cells (PBMCs) of BA patients and healthy controls (HC) were evaluated. The serum concentrations of IL-17a and IL-23 as well as Foxp3, IL-17a, ROR-γt, IL-6, IL-1β and TGF-β1 m-RNA and protein expressions in liver tissues and the number of Foxp3, IL-17a, ROR-γt, CD4 expressing cells which infiltrated the hepatic tissues were determined. The Th17/Treg cell ratio (P < 0.001) and blood concentrations of IL-17a and IL-23 (P < 0.05) were increased in the BA as compared to the HC group. Expressions of Foxp3, ROR-γt, IL-17a, IL-1β, IL-6 as well as TGF-β1 mRNA and proteins were significantly increased in BA as compared to HC livers (P < 0.01, P < 0.05). High levels of IL-17a/ROR-γt-positive and moderate levels of Foxp3-positive cells infiltrated damaged BA bile ducts and the ratio of FoxP3(+) T to CD4(+) T cells was significantly lower in BA than in HC samples (P < 0.01). Cytokine-induced imbalance between Th17 and Treg cells in BA livers may be involved in bile duct damage.
    Pediatric Surgery International 10/2013; 29(12). DOI:10.1007/s00383-013-3421-6 · 1.00 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Aquaporin-4 (AQP4) is the predominant water channel protein in the mammalian brain, and is mainly expressed in astrocytes. Besides its important role in water transport across the blood-brain barrier, our present study demonstrated that AQP4 deficiency impaired hippocampal long-term potentiation (LTP) and hippocampus-dependent memory formation, accompanied by the increase in extracellular glutamate concentration and N-methyl-D-aspartate (NMDA) receptor-mediated currents in hippocampal dentate gyrus (DG) region. The impairment of LTP and memory formation of AQP4 knockout (KO) mice was mediated by the downregulation of glutamate transporter-1 (GLT-1) expression/function, since it can be rescued by β-lactam antibiotic ceftriaxone (Cef), a potent GLT-1 stimulator. These results suggest that AQP4 functions as the modulator of synaptic plasticity and memory, and chronic Cef treatment rescues hippocampal memory deficit induced by AQP4 knockout.
    Neuropharmacology 08/2013; 75. DOI:10.1016/j.neuropharm.2013.08.009 · 5.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: For the double u-tube ground heat transfer process of the ground source heat pump, a three-dimensional heat transfer model was built based on the pipe thermal resistance among u-tube circuits. With different spacing of the double U-tube borehole and different operation modes, this study presents a numerical simulation analysis for the soil temperature around the double u-tube pipe. Results indicate that the soil temperature increases within the allowable range, with 6.5m tube well spacing and the system to run 130 days in winter. When the ground source heat pump system runs 140 days in winter, the soil temperature increasing lies the allowable range with different tube well spacing what is mentioned in this paper. With the same operation parameters, the soil temperature variation amplitude of the double u-tube mode is higher than the single u-tube mode’s, but the former’s deteriorating speed of soil temperature field is faster than the latter’s. Keywords: vertical double u-tube ground heat exchanger, temperature field, numerical simulation, different operation mode.
    International Conference on Civil, Transportation and Environmental Engineering (CTEE 12); 04/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Hirschsprung's disease (HSCR) is a complex developmental defect characterized by the absence of enteric ganglia in the gastrointestinal tract. Although the genetic defect of enteric nervous system (ENS) was identified to play a critical role in the progress of HSCR, the systemic genetic dissection of HSCR still needs to be clarified. In this study, we firstly performed exome sequencing of two HSCR patients from a Han Chinese family, including the affected mother and son. After the initial quality filtering (coverage ≥ 5X and SNP quality score ≥ 40) of the raw data, we identified 13,948 and 13,856 single nucleotide variants (SNVs), respectively. We subsequently compared the SNVs against public databases (dbSNP130, HapMap, and 1000 Genome Project) and obtained a total of 15 novel nonsynonymous SNVs in 15 genes, which were shared between these two patients. Follow-up Sanger sequencing and bioinformatics analysis highlighted variant c.853G>A (p.E285K) in NRG3, a gene involved in the development of ENS. In the validation phase, we sequenced all nine exons of NRG3 in 96 additional sporadic HSCR cases and 110 healthy individuals and identified another nonsynonymous variant c.1329G>A (p.M443I) and two synonymous variants c.828G>A (p.T276T) and c.1365T>A (p.P455P) only in the cases. Our results indicated that NRG3 may be a susceptibility gene for HSCR in a Chinese population.
    Molecular Neurobiology 01/2013; 47(3). DOI:10.1007/s12035-012-8392-4 · 5.14 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Oxidative stress has recently been considered as a pivotal player in the pathogenesis of diabetic gastrointestinal dysfunction. We therefore investigated the role of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-beta-D-glucoside (THSG) that has a strong anti-oxidant property, in diabetic gastrointestinal dysmotility as well as the underlying protective mechanisms. THSG restored the delayed gastric emptying and the increased intestinal transit in streptozotocin (STZ)-induced diabetic mice. Loss of neuronal nitric oxide synthase (nNOS) expression and impaired nonadrenergic, noncholinergic (NANC) relaxations in diabetic mice were relieved by long-term preventive treatment with THSG. Meanwhile, THSG (10(-7)∼10(-4) mol/L) enhanced concentration-dependently NANC relaxations of isolated colons in diabetic mice. Diabetic mice displayed a significant increase in Malondialdehyde (MDA) level and decrease in the activity of glutathione peroxidase (GSH-Px), which were ameliorated by THSG. Inhibition of caspase-3 and activation of ERK phosphorylation related MAPK pathway were involved in prevention of enhanced apoptosis in diabetes afforded by THSG. Moreover, THSG prevented the significant decrease in PPAR-γ and SIRT1 expression in diabetic ileum. Our study indicates that THSG improves diabetic gastrointestinal disorders through activation of MAPK pathway and upregulation of PPAR-γ and SIRT1.
    PLoS ONE 12/2012; 7(12):e50291. DOI:10.1371/journal.pone.0050291 · 3.23 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To report early and late results of laparoscopic-assisted endorectal Soave pull-through (LAEPT) with a short and V-shaped partial resection muscular cuff for Hirschsprung's disease (HD) over a 10-year period. The clinical courses of 218 patients who underwent modified LAEPT for HD were reviewed. LAEPT was described by the Georgeson technique. The main modifications included less dissection of the bottom of the pelvis, rectal mucosa dissection with a long cuff, coloanal anastomosis with a short cuff, and a V-shaped partial resection in the posterior wall of the muscular cuff. From September 1999 to July 2009, 218 patients were operated on by the same surgeon. Ages ranged from 15 days to 12 years old. The aganglionic segment was located in the rectum or sigmoid colon in 176 patients, in the left colon in 38 children, and in the transverse colon in 4 patients. The median operating time was 176 minutes. Conversion to open surgery was required in 2 patients. Bleeding of the left iliac vein occurred in 1 patient, and a 180° twist of the neorectum occurred in 2 cases. Median first bowel movement time was 23 hours, and median postoperative hospital stay was 10.4 days. Median daily defecation frequency was 4.6 within 2 weeks and 2.3 at 3 months postoperatively. The immediate postoperative complications included intestine herniation from the trocar site in 2 patients, perianal excoriation in 32 patients, and anastomotic leakage in 3 patients. Follow-up ranging from 6 to 120 months was obtained for 182 patients. Late postoperative complications included postoperative adhesive bowel obstruction (1.1%), enterocolitis (7.7%), anasomostic stenosis (2.2%), constipation (1.6%), and soiling (3.6%). Eighty-seven percent had excellent and good bowel function. LAEPT with a short muscular cuff with a V-shaped partial resection in the posterior wall is a safe and effective procedure for HD.
    Journal of Laparoendoscopic & Advanced Surgical Techniques 04/2012; 22(3):280-4. DOI:10.1089/lap.2011.0081 · 1.34 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND AND PURPOSE Ginsenoside Rg1 (Rg1) is one of the major bioactive ingredients of Panax ginseng with little toxicity and has been shown to have neuroprotective effects. In this study, we investigated the antidepressant-like effect of Rg1 in models of depression in mice. EXPERIMENTAL APPROACH The effects of Rg1 were assessed in the forced swimming test (FST) and tail suspension test (TST) in mice. Rg1 was also investigated in the chronic mild stress (CMS) mouse model of depression with imipramine as the positive control. Changes in hippocampal neurogenesis and spine density, the brain-derived neurotrophic factor (BDNF) signalling pathway, and serum corticosterone level after chronic stress and Rg1 treatment were then investigated. The tryptophan hydroxylase inhibitor and the tyrosine kinase B inhibitor were also used to explore the antidepressive mechanisms of Rg1. KEY RESULTS Ginsenoside Rg1 exhibited antidepressant-like activity in the FST and TST in mice without affecting locomotor activity. It was also effective in the CMS model of depression. Furthermore, Rg1 up-regulated the BDNF signalling pathway in the hippocampus and down-regulated serum corticosterone level during the CMS procedure. In addition, Rg1 was able to reverse the decrease in dendritic spine density and hippocampal neurogenesis caused by CMS. However, Rg1 had no discernable effect on the monoaminergic system. CONCLUSIONS AND IMPLICATIONS Our results provide the first evidence that Rg1 has antidepressant activity via activation of the BDNF signalling pathway and up-regulation of hippocampal neurogenesis.
    British Journal of Pharmacology 02/2012; 166(6):1872-87. DOI:10.1111/j.1476-5381.2012.01902.x · 4.84 Impact Factor
  • Jun Yang · Zhuang-Li Hu · Bo Jiang · Lan Ni · You Jin · Jian-Guo Chen · Fang Wang ·
    [Show abstract] [Hide abstract]
    ABSTRACT: Reactive oxygen species (ROS), including superoxide, are generally considered as neurotoxic molecules whose effects can be alleviated by antioxidant enzymes. However, ROS also are known to be necessary components of the signal transduction cascades underlying normal synaptic plasticity. The oxidant chloramine-T (Ch-T), a specific oxidant to sulphur-containing residues, can oxidize methionine (Met) residues in proteins to alter protein function. To investigate the effect of Ch-T on the induction of hippocampal long-term potentiation (LTP) in dentate gyrus (DG), in vivo electrophysiological recording was employed. It was found that intracerebroventricular (ICV) injection of 0.1 μM Ch-T in 5 μL enhanced hippocampal LTP of rats slightly, whereas, 20 mM Ch-T in 5 μL greatly attenuated LTP. These effects can be reversed by pretreatment with 0.1 mM dithiothretol (DTT), a special thiol reductant. In addition, 0.1 μM Ch-T elevated LTP-induced increase in phosphorylation of Ca²+/calmodulin (CaM)-dependent protein kinase (CaMKII) and neurogranin (Ng), whereas 2 μM and 20 mM Ch-T reduced LTP-induced increase in phosphorylation status of the two key proteins, especially for 20 mM Ch-T. Pretreatment with DTT significantly prevented these effects. Taken together, these findings demonstrated that Ch-T has concentration-dependent effects on the induction of hippocampal LTP in vivo. In brief, low concentration of Ch-T facilitated hippocampal LTP by enhancing LTP-induced increase in p-CaMKII and p-Ng compared to controls, whereas high concentration of Ch-T obviously attenuated LTP accompanied by a decrease in the phosphorylated proteins, and both of these effects can be prevented by DTT. These results indicate that Ch-T modulates hippocampal LTP through regulating phosphorylation status of CaMKII and Ng.
    NeuroToxicology 03/2011; 32(2):199-205. DOI:10.1016/j.neuro.2011.01.006 · 3.38 Impact Factor
  • Wei Jiang · Veng-cheong Lo · Bao-Dong Bai · Jun Yang ·
    [Show abstract] [Hide abstract]
    ABSTRACT: A molecular-based magnetic material AFeIIFeIII(C2O4)3 (A = organic cation) with a honeycomb structure is studied. The molecular-based magnet system consists of mixed spin-2 and spin- 5/2 honeycomb lattices with ferrimagnetic interlayer coupling. The magnetization, hysteresis loops and initial susceptibility have been calculated using a numerical method which includes both the longitudinal and transverse fields. We investigated the magnetic reversal of the system and found the existence of triple hysteresis loop patterns, affected by the anisotropy, longitudinal and transverse fields, and interlayer and intralayer exchange.
    Physica A: Statistical Mechanics and its Applications 06/2010; 389(11):2227–2233. DOI:10.1016/j.physa.2010.01.050 · 1.73 Impact Factor
  • Cheng-Bo Zhu · Wei Jiang · Veng-Cheong Lo · Jun Yang · Wei Wang ·
    [Show abstract] [Hide abstract]
    ABSTRACT: The magnetic properties of a three-layer Heisenberg model with spins at six sublattices are investigated by using retarded Green's function method and spin-wave theory. The spin-wave spectra of the system and the energy gap are calculated numerically, which are related to the magnetic symmetrical structure of the model. The quantum fluctuation at zero temperature, magnetization, internal energy and specific heat at low temperature are compared under different sets of intra- and interplane coupling interactions. The results show that the ferrimagnetic coupling plays an important role in various physical properties of the system.
    Physica B Condensed Matter 04/2010; 405(7):1677-1685. DOI:10.1016/j.physb.2009.12.066 · 1.32 Impact Factor

Publication Stats

105 Citations
28.41 Total Impact Points


  • 2013-2015
    • Wuhan Children's Hospital
      Wu-han-shih, Hubei, China
    • PLA University of Science and Technology
      Peping, Beijing, China
  • 2011-2013
    • Huazhong University of Science and Technology
      • • Department of Pediatric Surgery
      • • School of Basic Medicine
      Wu-han-shih, Hubei, China
  • 2010
    • Shenyang University of Technology
      Feng-t’ien, Liaoning, China